• Journal of the Korean Chemical Society
• /
• v.20 no.3
• /
• pp.185-192
• /
• 1976

Equivalent conductances of ammonium salts such as $NH_4Cl,\;CH_3NH_3Cl,\;(CH_3)_2NH_2Cl,\;(CH_3)_3NHCl\;and\;(CH_3)_4NCl$ have been measured in methanol-water mixtures of various composition (0.0${\sim}$0.6-mole frcation: m. f.) in the concentration range from 0.01 to 0.04 M at $25^{\circ}C$, and limiting equivalent conductances (${\Lambda}_0$) are determined by the analysis of Fuoss Onsager's semiempirical equation.The experimental results are discussed in terms of varing the size of ${\Lambda}_0$ due to the salts species and solvent composition. It has been observed in quite common over all salts that ${\Lambda}_0$ decreases with the increase of m. f. methanol, and the maximum decrement in ${\Lambda}_0$ shows between water and 0.1 m. f., while the plot of ${\Lambda}_0$ vs. m. f. goes through a minimum at 0.4 m. f. and then turns slightly upward. On the other hand, the ${\Lambda}_0$ decrease with the increase of the size of ammonium cation and the larger values ${\Lambda}_0$ are showed in comparison with those of values observed for the corresponding $n-RNH_3Cl$ under the same experimental procedure. All these behaviors are considered to be closely correlated with the structures of solvents and solutes and the significant similarities are compared with the behaviors of partial molar volumes for amine salts in same solvent system.

• Journal of Life Science
• /
• v.23 no.6
• /
• pp.751-756
• /
• 2013

This research old age chestnut tree that loss function chestnut leaf renewal efficiency enlargement and development of cosmeceutical product by purpose. Chestnut leaf (CL) exhibits numerous pharmacological effect including anti-allergy and anti-microbial properties. However, the anti-wrinkle effect of CL is not completely understood. In this study, to find a possible explanation for the anti-wrinkle effects of CL, we evaluated the effects of CL on radical scavenging activity, elastase inhibition activity and collagenase inhibition activity. CL was extracted with various solvents including water (CLW), 70% ethanol (CLE) and 70% acetone (CLA). The results showed that CLW, CLE, and CLA have the radical scavenging activity. In addition, we showed that CLW, CLE, and CLA have the elastase inhibition activity and collagenase inhibition activity. Consequently, the CL has a potent anti-wrinkle effect and it could be a useful cosmeceutical product for anti-aging purposes.

• Korean Journal of Medicinal Crop Science
• /
• v.26 no.2
• /
• pp.170-180
• /
• 2018

Background: To obtain useful cosmetic resources, this study aimed to determine the non-saponin fatty acid and inhibitory activities of collagenase and elastase by treatment of Saccharomyces cerevisiae in supercritical fluid extracted oil of the adventitious root culture of wild mountain ginseng. Methods and Results: We performed supercritical fluid extraction at various conditions such as pressure, temperature, time, and use of co-solvents, unlike the n-hexane extraction for the adventitious roots culture of wild mountain ginseng. The non-saponin-fatty acid obtained from the oil of the adventitious roots culture was incresed by treatment with S. cerevisiae. The supercritical fluid extraction was conducted using gas chromatography. Non-saponin-fatty acid content, in the oil of adventitious roots culture of wild mountain ginseng treated with S. cerevisiae for 2 days were three times higher than that in the control. In addition, the oil of the adventitious roots culture treated with S. cerevisiae was investigated for the anti-wrinkle effect by using collagenase and elastase. The oil of adventitious roots culture treated with S. cerevisiae exhibited higher collagenase and elastase inhibitory activities than those in the control. Conclusions: Supercritical fluid extracted oil of the adventitious roots culture of wild mountain ginseng treated with S. cerevisiae was found to have decreased ratio of saturated fatty acids and incresed ratio and content of unsaturated fatty acids increased. Furthermore, it showed anti-wrinkle effects in vitro.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.9
• /
• pp.1323-1331
• /
• 2017

Objective: This study investigated the effect of extraction solvents on antioxidant bio-active compounds as well as potential antioxidant and lipid peroxidation inhibition of Phyllanthus acidus (P. acidus) leaf extract in minced pork. Methods: The effect of various solvent systems of water, 25%, 50%, 75% (v/v) ethanol in water and absolute ethanol on the extraction crude yield, total phenolic content, total flavonoid content and in vitro antioxidant activities of P. acidus leaves was determined. In addition, antioxidant activities of the addition of crude extract from P. aciuds leaves at 2.5 and 5 g/kg in minced pork on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical cation decolorization, reducing power and inhibition of lipid peroxidation (thiobarbituric acid reactive substances; TBARS) were determined. Moreover, sensory evaluation of the samples was undertaken by using a 7-point hedonic scale. Results: The results showed that the highest crude yield (2.8 g/100 g dry weight) was obtained from water which also had the highest recovery yield for total phenolic content, total flavonoid content and the strongest antioxidant activity. The addition of crude water extract from P. acidus leaves was more effective in retarding lipid peroxidation and higher antioxidant activity than control and butylated hydroxytoluene in minced pork. In particular, the samples containing P. acidus extract had no significant effect on the sensory scores of overall appearance, color, odor, texture, flavor, and overall acceptability compared to the control. Conclusion: Water solvent was an optimally appropriate solvent for P. acidus leaf extraction because of its ability to yield the highest amount of bio-active compounds and in vitro antioxidant property. Particularly, P. acidus crude water extract also strongly expressed the capacity to retard lipid oxidation, radical scavenging, radical cation decolorization and reducing power in minced pork. The results of this study indicated that P. acidus leaf extract could be used as natural antioxidant in the pork industry.

• Journal of the Korean Applied Science and Technology
• /
• v.34 no.2
• /
• pp.400-409
• /
• 2017

The purpose of this study was to measure the antioxidant and physiological activities of seed from Gardenia jasminoides Ellis fructus (GJE) in Namhae Korea. We determined proanthocyanidin. GJE seed were extracted by 70% ethanol, chloroform:methanol, 2:1 volume ratio (CM) and n-butanol of three solvents. To investigate by the solvent extract of flavonoid content and value as a functional food ingredient of GJE seed through various antioxidant activities were performed. Solvent extract antioxidant activity of increasing concentrations (0.2, 0.4, 0.6 mg/mL) were significantly increased (p<0.05). The content of proanthocyanidin in GJE seed was $70.035{\pm}0.772mg$ catechin eqivalents/g dry weight. As a result of bioactivity assay, by a solvent of seed were found that the relationship with the increase of flavonoid content increased bioactivities. The antioxidant activity of the extract from the other except for the n-butanol extract on seed was observed at a high level. The results suggest that Gardenia jasminoides Ellis fructus seed can be used as a natural antioxidant.

• Journal of Life Science
• /
• v.25 no.10
• /
• pp.1169-1175
• /
• 2015

Although many studies have described the physiological effects of bee products, such as honey, propolis, pollen, and royal jelly, the health benefits of honeycomb remain incompletely characterized. We performed a comparative study of the antioxidant properties of honey and honeycomb extracts using two different solvents (water and 95% ethanol). The results showed that the total phenolic and flavonoid content of the honeycomb extract was higher than that of the honey extract. They also demonstrated that water was more effective than ethanol in extracting total phenols. The in vitro antioxidant properties of the water honeycomb extract were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO^•) radical scavenging assays and ferrous ion chelating and reducing power assays. The antioxidant activity of the honeycomb extract exhibited was higher than that of the honey extract. The 50% effective concentrations (EC₅₀) of the honeycomb extract were 7.3±0.26 mg/ml for scavenging DPPH radicals, 6.1±0.22 mg/ml for scavenging NO^• radicals, 6.9±0.44 mg/ml for chelating ferrous ions, and 8.2±0.11 mg/ml for reducing power. A correlation analysis revealed that the total phenolics and flavonoids of the honeycomb extract were the major contributors to the radical scavenging activity, ferrous ion chelating, and reducing power. The honeycomb extract was effective in protecting biological systems against various oxidative stresses in vitro. This is the first report on the antioxidant properties of honeycomb.

• Applied Chemistry for Engineering
• /
• v.27 no.4
• /
• pp.444-448
• /
• 2016

Hollow silicon/carbon (H-Si/C) composites as anode materials for lithium ion batteries were investigated to overcome the large volume expansion. H-Si/C composites were prepared as follows; hollow $SiO_2\;(H-SiO_2)$ was prepared by adding $NaBH_4$ to $SiO_2$ synthesized using $st{\ddot{o}}ber$ method followed by magnesiothermic reduction and carbonization of phenolic resin. The H-Si/C composites were analyzed by XRD, SEM, BET and EDX. To improve the capacity and cycle performance, the electrochemical characteristics of H-Si/C composites synthesized with various $NaBH_4$ contents were investigated by charge/discharge, cycle, cyclic voltammetry and impedance tests. The coin cell using H-Si/C composite ($SiO_2:NaBH_4=1:1$ in weight) in the electrolyte of $LiPF_6$ dissolved in organic solvents (EC : DMC : EMC = 1 : 1 : 1 vol%) has better capacity (1459 mAh/g) than those of other composition coin cells. It is found that the coin cell ($SiO_2:NaBH_4=1:1$ in weight) has an excellent capacity retention from 2nd cycle to 40th cycle.

• Food Science and Preservation
• /
• v.11 no.3
• /
• pp.352-357
• /
• 2004

The present study was conducted to investigate extraction characteristics and antioxidative activity of Lycium chinense extracts. Lycium chinense were extracted by reflux extraction(RE) under different extraction conditions including solvent. The solid yield, turbidity, color value, titratable acidity, free sugar contents, electron donating ability(EDA) and superoxide dismutase(SOD)-like ability of Lycium chinense extracts were determined. The highest solid yield value were obtained with water of 10 fold. No significant difference in turbidity and color value were found among the extracts prepared with various extraction solvents, 75$\%$ ethanol, 50$\%$ ethanol and water. The highest titratable acidity were obtained with water of Lycium chinense. The free sugar contents of Lycium chinense extracted with water showed the highest value. Lycium chinense extracts with water included higher contents of free sugar compared with those of the other solvent extracts, 50$\%$ ethanol and 75$\%$ ethanol extracts. The total polyphenol compounds content of Lycium chinense extracted with 50% ethanol showed the highest value. Lycium chinense extracts with 50$\%$ ethanol included higher contents of total polyphenol compound compared with those of the other solvent extracts, water and 75$\%$ ethanol extracts.

• KSBB Journal
• /
• v.23 no.5
• /
• pp.452-459
• /
• 2008

To obtain maximal efficacy with minimal systemic side-effects, many studies have been carried out to achieve the controlled release of 5-fluorouracil (5-FU). In this study, biodegradable poly(L-lactide) (L-PLA) microparticles containing 5-FU were prepared by a process, called aerosol solvent extraction system (ASES), utilizing supercritical carbon dioxide. The effects of various organic solvents, drug/polymer feeding ratio, polymer molecular weight, and blending with the same polymers with different molecular weights on the formation of 5-FU loaded microparticles were investigated under a predetermined operating condition from our previous study. The drug recovery, entrapment efficiency, and in vitro drug release kinetics were determined by HPLC assays. The drug recovery obtained from the ASES process was found to be very high, whereas the drug entrapment efficiency was considerably low in all the experiments due to the poor affinity between L-PLA and 5-FU. These results indicated that the precipitation rate of L-PLA might be quite different from that of 5-FU so that there was little chance to form 5-FU loaded L-PLA microparticles.

• Applied Chemistry for Engineering
• /
• v.29 no.3
• /
• pp.336-341
• /
• 2018

In general, the synthesis of poly(3-hexylthiophene)(P3HT)-based block copolymers requires at least a 4-5 step process. To control the molecular weight, molecular weight distribution, and block ratio, the reaction conversion and time should be monitored. In addition, the reaction scale usually limited to several mg to g was difficult to increase due to the limitations of living radical polymerizations. In this study, we synthesized P3HT-b-poly(4-vinylprydine) (P3HT-b-P4VP) with a final product quantity of > 19 g via a 2-step synthetic method with an anionic polymerization. In this method, the molecular weight and molecular weight distribution of P3HT-b-P4VP can be well controlled without monitoring the reaction conversion. We also studied physical properties of P3HT-b-P4VP depending on different solvent systems, which were investigated by UV-vis spectroscopy, atomic force microscopy, and ultraviolet photoelectron spectroscopy.