• Molecules and Cells
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• v.45 no.4
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• pp.257-272
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• 2022

In addition to inducing apoptosis, caspase inhibition contributes to necroptosis and/or autophagy depending on the cell type and cellular context. In macrophages, necroptosis can be induced by co-treatment with Toll-like receptor (TLR) ligands (lipopolysaccharide [LPS] for TLR4 and polyinosinic-polycytidylic acid [poly I:C] for TLR3) and a cell-permeable pan-caspase inhibitor zVAD. Here, we elucidated the signaling pathways and molecular mechanisms of cell death. We showed that LPS/zVAD- and poly I:C/zVAD-induced cell death in bone marrow-derived macrophages (BMDMs) was inhibited by receptor-interacting protein kinase 1 (RIP1) inhibitor necrostatin-1 and autophagy inhibitor 3-methyladenine. Electron microscopic images displayed autophagosome/autolysosomes, and immunoblotting data revealed increased LC3II expression. Although zVAD did not affect LPS- or poly I:C-induced activation of IKK, JNK, and p38, it enhanced IRF3 and STAT1 activation as well as type I interferon (IFN) expression. In addition, zVAD inhibited ERK and Akt phosphorylation induced by LPS and poly I:C. Of note, zVAD-induced enhancement of the IRF3/IFN/STAT1 axis was abolished by necrostatin-1, while zVAD-induced inhibition of ERK and Akt was not. Our data further support the involvement of autocrine IFNs action in reactive oxygen species (ROS)-dependent necroptosis, LPS/zVAD-elicited ROS production was inhibited by necrostatin-1, neutralizing antibody of IFN receptor (IFNR) and JAK inhibitor AZD1480. Accordingly, both cell death and ROS production induced by TLR ligands plus zVAD were abrogated in STAT1 knockout macrophages. We conclude that enhanced TRIF-RIP1-dependent autocrine action of IFNβ, rather than inhibition of ERK or Akt, is involved in TLRs/zVAD-induced autophagic and necroptotic cell death via the JAK/STAT1/ROS pathway.

• Journal of Biomedical Engineering Research
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• v.27 no.2
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• pp.78-82
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• 2006

The ventricular assist device(VAD) helps to reduce the overload against the patient's native heart(NH). The pulsatile VAD pumps out the ventricular blood to the aorta with pulsatile flow. If the VAD pulsates simultaneously with the NH, the ventricle of the NH could confronts abnormally elevated aortic pressure, and this could deteriorate the ventricle rather than assist to recover it. Thus counterpulsation algorithms to avoid copulsation have been adopted by many VADs, but these methods utilize electrocardiography or arterial pressure signals, which may have difficulties to acquire consistently for a long period. In this study, the copulsation estimation algorithm for the counterpulsation is developed using the VAD outlet pressure signal. The VAD outlet pressure signal is good to maintain for a long time and the sensor part could be integrated to the VAD as a built-in module. From the VAD outlet pressure signal and its pump rate information calculated with Fast Fourier Transform, pulse peaks by the VAD and the NH were extracted and the next copulsation time at which the VAD and the NH would pulsate simultaneously was estimated. This estimation algorithm was implemented by using PC MATLAB software and tested for various pump rate conditions with mock circulation system. For each condition, the copulsation time was estimated successfully. Consequently, the results showed the possibility to use the outlet cannula pressure signal in the copulsation estimation.

• Journal of Chest Surgery
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• v.32 no.12
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• pp.1065-1077
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• 1999

Background : Ventricular assist devices(VADs) are being used for patients in postcvardiotomy cardiogenic shock status bridge to cardiac transplant settings and in post-myocardial infarction cardiogenic shock. The VAD which was developed at the Deparment of medical engineering in Dankook University College of Medicine was a pneumatically driven device and can maintain pulsatile flow. The goal of this study is to develop animal experimental models using the VAD and to clarify the reliability and hemodynamic property adequacy of end organ perfusion durability and severity of thrombotic-hemolytic tendency of the device. Material and Method : The pneumatic VAD was applied to 8 adult female lambs, We examined some hemodynamic parameters such as arterial blood pressure pulmonary capillary wedge pressure(pcwp) pulmonary artery pressure(PAP) left atrial pressure hour urine output cardiac index VAD flow EKG to determine the reliability of the VAD and hemodynamic compatibility of the experimental animals within 24 hours of experiment. We also observed the end organ perfusion durability of the VAD and thrombotic-hemolytic property of the VAD after 24 hours of VAD insertion. Result: We could monitor all hemodynamic parameters including pcwp PAP cardiac index EKG, adn hour urine as true clinical settings. We observed that the reliability of the VAD was excellent and the hemodynamic property of the experimental animal and end organ perfusion were adequate within 24 hours of experiment. In four lambs surviving 24 hours after insertion the reliability of the VAD and end organ perfusion were excellent and no thrombotic-hemolytic tendency was noted. However after 15 days of experiment the diaphragm of the VAD was torn and it was recommende that the durability of the VAD should be extended. Conclusion : e conclude that the pneumatic VAD developed at Dankook University Biomedical Engineering has good hemodynamic property and low thromboembolic tendency and presents adequate end organ perfusion but we noted that the durability of the device should be expanded further. It will be possible to do more reliable experiment in the future according to the animal experimental method developed in this study especially with the heart failure models.

• The Journal of the Acoustical Society of Korea
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• v.22 no.4E
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• pp.161-166
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• 2003

We present an adaptive wavelet packet based speech enhancement method with robust voice activity detection (VAD) in non-stationary noise environment. The proposed method can be divided into two main procedures. The first procedure is a VAD with adaptive wavelet packet transform. And the other is a speech enhancement procedure based on the proposed VAD method. The proposed VAD method shows remarkable performance even in low SNRs and non-stationary noise environment. And subjective evaluation shows that the performance of the proposed speech enhancement method with wavelet bases is better than that with Fourier basis.

• Proceedings of the Acoustical Society of Korea Conference
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• autumn
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• pp.19-22
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• 2000

ITU-T 국제 표준화 기구에서 인터넷 폰과 화상회의를 목적으로 개발된 G.723.1 음성 부호화기는 잡음 구간에서의 전송률을 낮추기 위한 방법으로 VAD(Voice Activity Detector)와 CNG(Comfortable Noise Generator)를 사용하고 있다. 이중 VAD는 최종적으로 현재 프레임의 에너지 레벨을 비교하여 음성의 활동 유무를 판정하고 있다. 하지만 G.723.1 VAD에서는 보다 안정적인 판정을 위해 음성 활동 구간 사이에 삽입되어 있는 묵음 구간에 대해서는 거의 대부분 음성이 활동하는 영역으로 판정을 하고 있다. 따라서 본 논문에서는 묵음 구간에 대해 보다 정확한 판정을 통하여 기존의 방법에 비해 전송률을 더욱 감소시킬 수 있는 방법을 제안한다. 제안한 방법은 음성신호와 잡음신호의 LSP 파라미터 간격 정보를 이용하여 음성구간을 검출한다. 묵음구간을 길게 조절한 문장을 사용하여 실험한 결과 VAD=1로 판정한 프레임수가 약 $48.98\%$ 감소하였으며 주관적인 음질평가의 경우 음질의 열하는 거의 발생하지 않았다.

• Proceedings of the KSPS conference
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• 2005.11a
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• pp.59-62
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• 2005

Voice activity detection (VAD) is important in many areas of speech processing technology. Speech/nonspeech discrimination in noisy environments is a difficult task because the feature parameters used for the VAD are sensitive to the surrounding environments. Thus the VAD performance is severely degraded at low signal-to-noise ratios (SNRs). In this paper, a new VAD algorithm is proposed based on the degree of voicing and Quantile SNR (QSNR). These two feature parameters are more robust than other features such as energy and spectral entropy in noisy environments. The effectiveness of proposed algorithm is evaluated under the diverse noisy environments in the Aurora2 DB. According to out experiment, the proposed VAD outperforms the ETSI Advanced Frontend VAD.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.12C
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• pp.1262-1267
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• 2005

Elliptical basis function (EBF) neural network works stably under high-level background noise environment and makes the nonlinear processing possible. It can be adapted real time VAD with simple design. This paper introduces VAD implementation using EBF and the experimental results show that EBF VAD outperforms G729 Annex B and RBF neural networks. The best error rates achieved by the EBF networks were improved more than $70\%$ in speech and $50\%$ in silence while that achieved by G.729 Annex B and RBF networks respectively.

• International Journal of Internet, Broadcasting and Communication
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• v.11 no.4
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• pp.26-30
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• 2019

This paper proposes a new voice activity detection (VAD) method which is based on SNR and non-intrusive speech intelligibility estimation. In the conventional SNR-based VAD methods, voice activity probability is obtained by estimating frame-wise SNR at each spectral component. However these methods lack performance in various noisy environments. We devise a hybrid VAD method that uses non-intrusive speech intelligibility estimation as well as SNR estimation, where the speech intelligibility score is estimated based on deep neural network. In order to train model parameters of deep neural network, we use MFCC vector and the intrusive speech intelligibility score, STOI (Short-Time Objective Intelligent Measure), as input and output, respectively. We developed speech presence measure to classify each noisy frame as voice or non-voice by calculating the weighted average of the estimated STOI value and the conventional SNR-based VAD value at each frame. Experimental results show that the proposed method has better performance than the conventional VAD method in various noisy environments, especially when the SNR is very low.

• Proceedings of the Acoustical Society of Korea Conference
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• autumn
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• pp.47-48
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• 2004

최근 음성시장은 VoiceXML 을 이용하여 간단히 시나리오를 작성할 수 있어, 보다 쉽게 다양한 어플리케이션을 개발하여 서비스할 수 있게 되었다. HUVOIS-VAD 는 KT가 자체 개발한 음성인식/합성 플랫폼 시스템인 HUVOIS 에 VXML 을 이용하여 시나리오를 구성한 음성인식/합성 VAD 시스템으로, 사내 및 사외 여러 사이트에 설치 운용 중이다. 특히, Web 을 기반으로 운용시스템을 구축하여 운용자는 어느 곳에서나, 운용 DB를 점검하거나, 인식사전을 구성하고, 운용할 수 있으며, 사용자 또한 개인의 DB 를 직접 관리하도록 하고, 회사의 DB 와 연계하여 개인의 VAD로 동시에 사용할 수 있도록 하고 있다. 본 논문에서는 Web 을 기반으로 구성된 HUVOIS-VAD 시스템에 대하여 기술하고자 한다.

• Journal of Biomedical Engineering Research
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• v.12 no.1
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• pp.29-34
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• 1991

Ventricular assist device(VAD) has been clinically applied as a temporary circulatory sup- porting system in the patients with severe heart failure, but small sized VAD for infant is not available. The purpose of tilis paper is to introdIAce small sized VAD and presents the result of in vitro test. Sejong VAD is diaphragmatic type of pneumatic pump and stroke volume is 11cc. Cardiac outputs of the Sejong VAD were measured by overflow tank under variable conditions of driving parameters. The cardiac output was 1.3 1/min at the heart rate of 120 per minute, left atrial pressure of $15cmH_2O$, percent systole of 43%, driving pressure of 240 mmHg, vacuum pressure of -40 mmHg, and mean aortic pressure of 70 mmHg. No mechanical problem was developed during the continuous in vitro test for 3 months.