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Pathological and molecular comparisons of five distinct species of pepper-infecting Potyviruses (oral)

  • Yoon, H.I.;Chung, H.M.;Ryu, K.H.
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.113.2-114
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    • 2003
  • Five pepper-infecting potyviruses, Pepper mottle virus (PepMoV), Chilli veinal mottle virus (CVMV), Pepper veinal mottle virus (PVMV), Pepper severe mosaic virus (PSMV) and Tobacco each virus (TEV), are known filamentous virus and can be infected pepper crops systemically. To understand pathology and genome information of the five viruses on pepper plants, host reactions and sequences were compared to the 5 viruses. Five potyviruses were inoculated onto some typical cultivars of hot peppers and compared their symptoms, and virus accumulations. A set of degenerate primers for potyviruses were applied to 5 viruses and RT-PCR was performed. RT-PCR products containing partial nuclear inclusion b and coat protein (CP) genes were cloned. Then, oligo dT primer and species-specific primer were redesigned to amplify the C-terminal part of CP and 3' noncoding regions of each viruses. Sequences of the viruses were analyzed and compared to serological relationships among the viruses. The data can be useful for screening of potyviruses in pepper plants and pathogen-derived transgenic pepper plant development.

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Characterization of PVDF/PU fibers prepared by electrospinning

  • Rho, Jeongwon;Lee, Deuk Yong;Lee, Myung-Hyun;Kim, Bae-Yeon;Jeong, Heeseok
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.28 no.1
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    • pp.1-8
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    • 2018
  • The 23 wt% polyvinylidene fluoride (PVDF)/15 wt% polyurethane (PU) fibers were electrospun using the conjugated nozzle at a flow rate of 1.0 mL/h and an electric field of 1 kV/cm. The formation of ${\beta}$ crystal phase in the PVDF and the PVDF/PU fibers was confirmed by Fourier transform infrared spectroscopy. After electrospinning, the asspun fibers were immersed in a boiling water and then dried at $100^{\circ}C$ in a convection oven to make a crimp phenomenon. The crimps with a diameter of $2.0{\pm}0.08{\mu}m$ were observed for the PVDF/PU fibers after hydrothermal treatment without sacrificing the extent of ${\beta}$ crystal phase. All the PU, PVDF and PVDF/PU fibers exhibited average cell viability of more than 98 %. The cell proliferation results suggested that L-929 cells adhered well to the PU, PVDF and PVDF/PU fibers and proliferated continuously with increasing time, indicating that the PVDF/PU fibers are highly applicable to the biomedical applications.

A Study on the Effect of Initial Strain on Cyclic Creep Properties of Steam Turbine Rotor Steel (화력 발전용 로터강의 초기 변형율이 CYCLIC 크리프 특성에 미치는 영향에 관한 연구)

  • 오세규;정순억;한상덕
    • Journal of Ocean Engineering and Technology
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    • v.6 no.1
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    • pp.78-86
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    • 1992
  • The creep behaviors of 1%Cr-Mo-V and 12%Cr steam turbine rotor steels under static or cyclic load were examined at 600 and $700^{\circ}C$. The relationship between these two kinds of phenomena was studied and the experimental results were summarized as follows: 1) It is confirmed that the cyclic creep strain dependent on time is more available for creep, behavior analysis according to frequency change than that dependent on number of cycles, and the static creep, the special case of cyclic creep with stress ratio of 1 can be also more effectively analyzed by time-dependence. 2) The steady cyclic creep rate vs. the steady static creep rate, increases according to the increase of stress ratio, and this phenomena may occur on occasion of the decrease of the internal stress. 3) The initial strain affects on all the creep properties of the transient region, the steady state region and the rupture time in cyclic creep as well as static creep, and the quantitative relationships among them exist.

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Globular Clusters in the Brightest Coma Spiral Galaxy NGC 4921 and the Distance to the Coma Cluster

  • Jang, In Sung;Lee, Myung Gyoon
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.2
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    • pp.34.1-34.1
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    • 2015
  • Deep archival V and I image data taken with Hubble Space Telescope have been used to investigate compact stellar objects in an anemic spiral galaxy NGC 4921 in the Coma cluster. We resolve a significant fraction of globular clusters based on the reconstructed master drizzled image data. The color distribution of globular clusters (GCs) shows a clear bimodal distribution. The blue and red GC populations show significantly different radial number density profiles. We derive the turnover magnitudes of globular cluster luminosity functions (GCLFs) for the blue and red GCs in the bulge and halo of NGC 4921. We also derive the GCLFs of two Coma cD galaxies, NGC 4874 and NGC 4889, and one coma S0 galaxy, NGC 4923. Turnover magnitudes of GCs in four galaxies agree well within uncertainties. A mean distance of four Coma galaxies is derived from turnover magnitudes of GCLFs. A value of the Hubble constant is determined from this distance estimate and radial velocity of the Coma. We discuss implications of our results in relation with the recent determinations of the Hubble constant.

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A 5-GHz Band CCNF VCO Having Phase Noise of -87 dBc/Hz at 10 kHz Offset

  • Lee, Ja-Yol;Lee, Sang-Heung;Kang, Jin-Young;Kim, Bo-Woo;Oh, Seung-Hyeub
    • Journal of electromagnetic engineering and science
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    • v.4 no.3
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    • pp.137-142
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    • 2004
  • In this paper, we present a new current-current negative feedback(CCNF) differential voltage-controlled oscillator (VCO) with 1/f induced low-frequency noise suppressed. By means of the CCNF, the 1/f induced low-frequency noise is removed from the proposed CCNF VCO. Also, high-frequency noise is stopped from being down-converted into phase noise by means of the increased output impedance through the CCNF and the feedback capacitor $C_f. The proposed CCNF VCO represents 11-dB reduction in phase noise at 10 kHz offset, compared with the conventional differential VCO. The phase noise of the proposed CCNF VCO is measured as - 87 dBc/Hz at 10 kHz offset frequency from 5.5-GHz carrier. The proposed CCNF VCO consumes 14.0 mA at 2.0 V supply voltage, and shows single-ended output power of - 12 dBm.

Parametric Study of AC Current Lead for the Termination of HTS Power Cable

  • Kim, D.L;Kim, S.H.;S. Cho;H.S. Yang;Kim, D.H.;H.S. Ryoo;K.C. Seong
    • Progress in Superconductivity and Cryogenics
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    • v.5 no.1
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    • pp.107-110
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    • 2003
  • High Temperature Superconductor (HTS) transmission cable can carry more than 2 to 5 times higher electricity and also obtain substantially lower transmission losses than conventional cables. Liquid nitrogen is to be used to cool the HTS power cable and its cost is much cheaper than the liquid helium used for the cooling of metal superconducting wire. In Korea the HTS power cable development project has been ongoing since July, 2001 with the basic specifications of 22.9kV, 50MVA and told dielectric type as the first 3-year stage. The cryogenic system of the HTS cable is composed of HTS cable cryostat termination and refrigeration system. Termination of HTS cable is a connecting part between copper electrical cable at room temperature and HTS cable at liquid nitrogen temperature. In order to design the termination cryostat, it is required that the conduction heat leak and Joule heating on the current lead be reduced, the cryostat be insulated electrically and good vacuum insulation be maintained during long time operation. Heat loads calculations on the copper current lead have been performed by analytical and numerical method and the feasibility study fer the other candidate materials has also been executed.

The SH3 Domain of Phospholipase C-${\gamma}1$ Associates with Shc

  • Kim, Myung-Jong;Hwang, Jong-Ik;Chang, Jong-Soo;Ryu, Sung-Ho;Suh, Pann-Ghill
    • BMB Reports
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    • v.32 no.2
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    • pp.119-126
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    • 1999
  • The SH3 domain of PLC-${\gamma}1$ has been known to induce DNA synthesis. However, little is known about the putative effector proteins that associate with the domain. In this report, we provide evidence that the SH3 domain of PLC-${\gamma}1$ associates with Shc, which has been implicated in the activation of p21Ras in response to many growth factors. The association between Shc and PLC-${\gamma}1$ is enhanced either by v-Src-induced transformation or EGF-stimulation in vivo and in vitro. Furthermore, from transient expression studies with COS-7 cells, we show that the SH3 domain of PLC-${\gamma}1$ is required for association with Shc in vivo, whereas tyrosyl phosphorylation of PLC-${\gamma}1$ is not. Taken together, we suggest that Shc might be involved in the PLC-${\gamma}1$-mediated signaling pathway.

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Measurement of Glass-Silicon Interfacial fracture Toughness and Experimental Evaluation of Anodic Bonding Process based on the Taguchi Method (다구찌 방법에 의한 유리-실리콘 양극접합 계면의 파괴인성치 측정 및 양극접합공정 조건에 따른 접합강도 분석)

  • Kang, Tae-Goo;Cho, Young-Ho
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.26 no.6
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    • pp.1187-1193
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    • 2002
  • Anodic bonding process has been quantitatively evaluated based on the Taguchi analysis of the interfacial fracture toughness, measured at the interface of anodically bonded silicon-glass bimorphs. A new test specimen with a pre-inserted blade has been devised for interfacial fracture toughness measurement. A set of 81 different anodic bonding conditions has been generated based on the three different conditions for four different process parameters of bonding load, bonding temperature, anodic voltage and voltage supply time. Taguchi method has been used to reduce the number of experiments required for the bonding strength evaluation, thus obtaining nine independent cases out of the 81 possible combinations. The interfacial fracture toughness has been measured for the nine cases in the range of 0.03∼6.12 J/㎡. Among the four process parameters, the bonding temperature causes the most dominant influence to the bonding strength with the influence factor of 67.7%. The influence factors of other process parameters, such as anodic voltage and voltage supply time, bonding load, are evaluated as 18%, 12% and 2.3%, respectively. The maximum bonding strength of 7.23 J/㎡ has been achieved at the bonding temperature of 460$\^{C}$ with the bonding load of 45gf/㎠, the applied voltage of 600v and the voltage supply time of 25minites.

The Surface Modification and Low Cycle Fatigue Behavior of N+ion Implantated 7050Al Alloy (질소 이온 주입시킨 7050Al합금의 표면 미세구조 변화와 저주기 피로거동)

  • Lee, C.W.;Kwun, S.I.
    • Journal of the Korean Society for Heat Treatment
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    • v.7 no.4
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    • pp.307-317
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    • 1994
  • The surf ace microstructure modification by $N^+$ ion implantation into 7050Al alloy and its low cycle fatigue behavior were investigated. Ion implantation method is to physically implant accelerated ions to the surface of a substrate. High dose of nitrogen($5{\times}10^{17}ions/cm^2$) were implanted into 7050Al alloy using current density of accellerating voltage of 100KeV. The implanted layers were characterized by Electron Probe-Micro Analysis(EPMA), Auger Elecron Spectroscopy(AES), X-Ray Diffraction(XRD), X-Ray Photoelectron Spectroscopy(XPS), and Transmission Electron Microscopy(TEM). The experimental results were compared with computer simulation data. It was shown that AlN was formed to 4500 ${\AA}$ deep. The low cycle fatigue life of the $N^4$ion modified material was prolonged by about three times the unimplanted one. The improved low cycle fatigue life was attributed to the formation of AlN and the damaged region on the surface by $N^+$ ion implantation.

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Factors Influencing Cardiopulmonary Resuscitation Skills in Nursing Students (간호대학생의 기본심폐소생술 수행능력 관련 요인)

  • Sim, Hwan-Hui;Tae, Young Sook
    • The Journal of Korean Society for School & Community Health Education
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    • v.16 no.2
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    • pp.1-15
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    • 2015
  • Objectives: This study purposed to identify the factors influencing cardiopulmonary resuscitation(CPR) skills in nursing students. Methods: The research design was descriptive study. Data were collected through structured questionnaires. The participants of this study were 202 nursing students in B & U metropolitan city, and C city. Data were collected from November 15 to November 30, 2013. The data were analyzed using t-test, ANOVA, Scheffe's test, Pearson's correlation coefficients, and Multiple stepwise regression with SPSS WIN v 20.0. Results: CPR knowledge(r=0.51, p<0.001), critical thinking disposition(r=0.43, p<0.001), CPR attitude(r=0.56, p<0.001). self efficacy(r=0.74, p<0.001), and CPR skills were significantly correlated with each other. Predictors of CPR skills were self efficacy, CPR knowledge, critical thinking disposition, grade, and CPR attitude. These variables explained 60% of CPR skills. Multiple regression analysis showed that most powerful factors influencing CPR skills among nursing students was self efficacy. Conclusions: Based on this study, it is required to develop nursing education program to improve self efficacy of nursing students for increasing CPR skills.