• Title/Summary/Keyword: Two culture

Search Result 5,785, Processing Time 0.035 seconds

Breeding of a new cultivar of White Flammulina velutipes, 'Seolhan' (흰색 팽이 신품종 '설한' 육성)

  • Im, Ji-Hoon;Oh, Minji;Oh, Youn-Lee;Kim, Minseek;Lee, Yun-Sang
    • Journal of Mushroom
    • /
    • v.19 no.4
    • /
    • pp.279-284
    • /
    • 2021
  • Flammulina velutipes is the representative edible mushroom in Korea. It makes up around 18% of the total mushroom production and accounts for about 36% of the total mushroom export. Despite being one of the most industrially important mushroom in Korea, the distribution rate of domestic cultivar was low (~31.6%) in 2020. Therefore, we need a new domestic cultivar with high quality as the main export mushroom. We developed a new white domestic cultivar, 'Seolhan', bred by mating two monokaryotic strains isolated from Baekseung and Woori-1ho. The optimum mycelial growth temperature was 25℃ and Seolhan had higher mycelial growth than the control at 20 and 25℃. In bottle cultivation, the optimum temperature of incubation was from 15℃ to 17℃ and the growth temperature of Seolhan was 7-8℃, which was slightly higher than that of the control. The yield of Seolan was 226.5±8.0 g, an increase of 18.4% compared to the control. The period of primodia formation also was a couple of days ahead. In morphological traits, the pileus of Seolhan was smaller than that of the control, the stipe was thicker, and the color brighter. As the results of replacement culture and genetic diversity test, it was confirmed that Seolhan was genetically different from other white varieties.

Anti-inflammatory Effect of Yukil-san Water Extract on LPS-induced RAW 264.7 Cells (LPS로 활성화된 RAW 264.7 cell에서 NF-𝜅B억제를 통한 육일산(六一散) 물추출물의 염증억제효과)

  • Lee, Chang Wook;Park, Sang Mi;Kim, Eun Ok;Byun, Sung Hui;Kim, Sang Chan
    • Herbal Formula Science
    • /
    • v.30 no.2
    • /
    • pp.45-57
    • /
    • 2022
  • Objectives : Yukil-san (YIS, 六一散; Liu yi san) is composed of Talcum and Glycyrrhizae Radix, the name is said to be derived from the proportion of the two herbal components of the formula. The YIS originated from 'Formulas from the discussion illuminating the Yellow Emperor's Basic Question'(黃帝素問宣明論方; Huang di su wen xuan ming lun fang) written by Liu Wan-Su (劉完素). YIS could clear summerheat, resolve dampness, and augment the qi. This formula may be used to treat the common cold, influenza, acute gastroenteritis, cystitis, urethritis and bacillary dysentery. But, there is insufficient of study about the effects of YIS on the anti-inflammatory activities. The present study evaluated the anti-inflammatory effects of YIS on lipopolysaccharide (LPS)-activated RAW 264.7 cells. Methods : Cell viability was assessed by MTT assay and nitric oxide (NO) was evaluated by measuring the nitrite content in culture medium. Pro-inflammatory cytokines such as tumor necrosis factor-α, interleukin-1β and IL-6 were quantified by ELISA kit. The expression of proteins related with nuclear factor-κB (NF-κB) pathway and inducible NO synthase (iNOS) were assessed by western blot analysis. Results : YIS significantly inhibited the expression of iNOS increased by LPS, and thus significantly inhibited the production of NO. In addition, YIS significantly inhibited pro-inflammatory cytokines. In the regulation of inflammation, NF-κB pathway plays a crucial role. YIS inhibited the expression of p-IκBα and thus inhibited the translocation of NF-κB to the nucleus. Conclusions : These results suggest that YIS ameliorates inflammatory response in LPS-activated RAW 264.7 cells through the inhibition of inflammatory mediators, via suppression of the NF-κB pathway. Therefore, this study provides objective evidence for the anti-inflammatory effect of YIS including the underlying mechanisms.

Variables Affecting Chinese University Students' Selection Factors for Korean Drama (중국 대학생의 한국 드라마 선택요인에 영향을 미치는 변인)

  • Liu, Li;Baek, Jongnam
    • Journal of the Korea Convergence Society
    • /
    • v.12 no.5
    • /
    • pp.267-274
    • /
    • 2021
  • This study was designed to investigate the variables affecting Chinese university students' selection factors for Korean drama. Participants in this study were 379 university students from Hunan Province, China. An online survey program(www.wjx.cn) was used to collect data for this study. The tool of this study was 'a tool for measuring the selection factors of Korean Dramas', and it was composed of two areas: completeness of work and recognition. The results of this study are as follows: First, the completeness of work factor are higher than the recognition of Korean dramas by Chinese university students. Second, there are differences in the factors for selection Korean dramas according to the gender, grade, age, major, and Korean Drama watching frequency of Chinese university students. Third, when Chinese university students select Korean Dramas, their preferred genre influences the selection factors for the level of work completion and recognition. Finally, it was discussed according to the results of this study, and suggested for the qualitative re-leap of Korean Dramas in the global era. In order to expand the Korean Wave market in China, cultural contents must be developed to embrace the sentiment of Chinese university students, a propagation strategy that reflects the latest consumption trends of Chinese youth must be established, and the sophisticated visual beauty of Korean Dramas must be realized.

A Delphi Study for Deriving Directions for Future Elementary School Science Textbook (미래 초등 과학 교과용도서 방향성 도출을 위한 델파이 연구)

  • Chae, Dong-Hyun;Shin, Jung-Yun;Kim, Eun-Ae
    • Journal of the Korean Society of Earth Science Education
    • /
    • v.14 no.1
    • /
    • pp.59-68
    • /
    • 2021
  • The purpose of this study is to provide basic data to derive the direction of future elementary science curriculum books through delphi study of science education experts. To this end, a panel of 18 experts was formed and two delphi investigations were conducted. By analyzing the mean, median, and CVR values for each item in the Delphi survey, the priorities of changes in science education for the future society and the validity of each item's implementation method were verified. In addition, by synthesizing this, the direction of future elementary science textbooks was derived. As a result, the future elementary science textbook can be 'fun and interesting science study', 'exploration performance-oriented learning' and 'science that enjoys and participates even as an adult'. It should be developed to prepare for culture. For this, it is necessary to use materials in real life, and it is necessary to present an experiment that stimulates curiosity and easy access using materials and preparations with high accessibility. In addition, it is necessary to develop a textbook for learning that science is a discipline that is highly connected with real life, and that it is also related to future career paths.

Text Mining Analysis of News Articles Related to 'Space Hazard' ('우주 위험' 관련 뉴스 기사의 텍스트 마이닝 분석 연구)

  • Jo, Hoon;Sohn, Jungjoo
    • Journal of the Korean earth science society
    • /
    • v.43 no.1
    • /
    • pp.224-235
    • /
    • 2022
  • This study aimed to confirm the status of media reports on space hazards using topic modeling analysis of media articles that are related to space hazards for the past 12 years. Therefore, Latent Dirichlet Allocation (LDA) analysis was performed by collecting over 1200 space hazards articles between 2010 and 2021 on solar storm, artificial space objects, and natural space objects from BIGKins news platform. The articles related to solar storm focused on three topics: the effect of solar explosion on satellites; effect of solar explosion on radio communication in Korea, centered on the Korean Space Weather Center; and relationship between aircrew and space radiation. The articles related to artificial space objects focused on three topics: the threat of space garbage to satellite and space stations and the transition of useful objects into space junk; the relationship between space garbage and humanity as shown in movies; and the effort of developed countries for tracking, monitoring, and disposing of space garbage. The articles related to natural space objects focused on two topics: International Space Agency's tracking and monitoring of near-Earth asteroids and the countermeasures of collisions, and the evolution and extinction of dinosaurs and mammals, with a focus on the collisions of asteroids or comets. Therefore, this study confirmed that domestic media play a role in conveying dangers of space hazards and arousing the attention of public using a total of eight themes in various fields such as society and culture, and derived education method and policy on space hazards.

Characterization of exopolysaccharide-producing lactic acid bacteria from Taiwanese ropy fermented milk and their application in low-fat fermented milk

  • Ng, Ker-Sin;Chang, Yu-Chun;Chen, Yen-Po;Lo, Ya-Hsuan;Wang, Sheng-Yao;Chen, Ming-Ju
    • Animal Bioscience
    • /
    • v.35 no.2
    • /
    • pp.281-289
    • /
    • 2022
  • Objective: The aim of this study was to characterize the exopolysaccharides (EPS)-producing lactic acid bacteria from Taiwanese ropy fermented milk (TRFM) for developing a clean label low-fat fermented milk. Methods: Potential isolates from TRFM were selected based on the Gram staining test and observation of turbid suspension in the culture broth. Random amplified polymorphic DNA-polymerase chain reaction, 16S rRNA gene sequencing, and API CHL 50 test were used for strain identification. After evaluation of EPS concentration, target strains were introduced to low-fat milk fermentation for 24 h. Fermentation characters were checked: pH value, acidity, viable count, syneresis, and viscosity. Sensory evaluation of fermented products was carried out by 30 volunteers, while the storage test was performed for 21 days at 4℃. Results: Two EPS-producing strains (APL15 and APL16) were isolated from TRFM and identified as Lactococcus (Lc.) lactis subsp. cremoris. Their EPS concentrations in glucose and lactose media were higher than other published strains of Lc. lactis subsp. cremoris. Low-fat fermented milk separately prepared with APL15 and APL16 reached pH 4.3 and acidity 0.8% with a viable count of 9 log colony-forming units/mL. The physical properties of both products were superior to the control yogurt, showing significant improvements in syneresis and viscosity (p<0.05). Our low-fat products had appropriate sensory scores in appearance and texture according to sensory evaluation. Although decreasing viable cells of strains during the 21-day storage test, low-fat fermented milk made by APL15 exhibited stable physicochemical properties, including pH value, acidity, syneresis and sufficient viable cells throughout the storage period. Conclusion: This study demonstrated that Lc. lactis subsp. cremoris APL15 isolated from TRFM had good fermentation abilities to produce low-fat fermented milk. These data indicate that EPS-producing lactic acid bacteria have great potential to act as natural food stabilizers for low-fat fermented milk.

Positive effect of Saengmaeg-san intake on blood lipid and arteriosclerosis index during high-intensity training (고강도 트레이닝 시 생맥산 섭취가 혈중 지질 및 동맥경화 지수에 미치는 긍정적인 영향)

  • Kwak, Jae-Jun;Lee, Jae-Hoon;Yook, Jang Soo;Lee, Sang-Ho;Ha, Min-Seong
    • Journal of the Korean Applied Science and Technology
    • /
    • v.38 no.6
    • /
    • pp.1512-1523
    • /
    • 2021
  • The purpose of this study was to investigate the effect of Saengmaeg-san intake on blood lipids and arteriosclerosis index in male tennis players. Seventeen male tennis players enrolled in university were assigned to a Saengmaeg-san intake group (n=9) and a placebo control group (n=8) according to whether they took Saengmaeg-san during 4-week high-intensity tennis training. Tennis training was conducted 5 times a week for 4 weeks, and the exercise intensity was performed at 70-90% of the spare heart rate. The intake of Saengmaeg-san was 110ml at a time before breakfast, before, during, during, and after exercise, and after dinner, a total of 7 times, 770ml per day. For all data, mean and standard deviation were used. Two-way repeated measures ANOVA was used to examine the effect of time and treatment, and Pearson's Correlation was used to examine the relevance of differences in blood lipid responses after treatment. As a result of this study, the Saengmaeg-san intake group significantly improved blood lipids (triglyceride, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol) and arteriosclerosis index, and there was a significant correlation appeared between ∆TG, ∆LDL-C, and ∆TC. As a result, the intake of Saengmaeg-san during high-intensity training of male college student tennis players can have a positive effect on blood lipids and arteriosclerosis index, suggesting that it can be an effective sports drink as an exercise supplement.

A Comparison of Chunhyang's Character in the 30th Edition of and Theatre Play (경판30장본<춘향전>과 연극<성춘향>에 나타난 춘향의 인물 성격 비교)

  • Pyo, Won-Soub;Kim, Jung-Hyun
    • Journal of Korea Entertainment Industry Association
    • /
    • v.14 no.7
    • /
    • pp.227-237
    • /
    • 2020
  • In the 30th edition of , Chunhyang is conventionally presented as a woman who tries to protect her chastity and it eventually bears the fruits of her love. On the other hand in , Chunhyang is reinterpreted as a woman who strives to protect feminist self-consciousness and beliefs, even in unreasonable social situations. By comparing the two different works, we can see differences in the behavior of the characters in how to overcome various obstacles, such as irregularity, gender discrimination, and identity conflict. By reinterpreting the emotions of the characters in the classic novel into the emotions of the modern characters, we can see the how the characters grow when trying to overcome the situation and find a solution, even though there is no change yet. This can be seen as the emotions of the characters become different as time goes by. As the times go by, the world's values and inclinations also change, and the irrationalities of the world will also go in various directions. Through the fusion of culture into history, even though it's irrational but we become familiar with it and will clearly understand the absurdities, and we can look forward to changes in the process.

A Critical Study of Local Cultural Policy by Village Mural (마을벽화로 본 지역문화정책 비판적 고찰)

  • Jeong, Yoonsoo
    • 지역과문화
    • /
    • v.7 no.3
    • /
    • pp.81-108
    • /
    • 2020
  • This study aims to critically consider the local cultural policies of the government and local governments by village mural. Village murals, which began to be painted in the late 1990s, have been a nationwide cultural phenomenon for decades. In particular, it was consistently used as an important element of various local cultural policies implemented by the government and local governments. The government and local governments have been working on the project for two purposes: 'improving the residential environment' and 'activating tourism' but it has largely focused on 'activating tourism'. This study grasped the concrete status of major village murals across the country through field surveys, one of the case study methods. Also, through the analysis of official documents, the government and local governments operating in the village mural project were analyzed for the policy basis and administrative management plan. As a result of on-site surveys, most of the village murals across the country had problems such as uniform subject matter, low level representation, and poor follow-up management. This is partly based on the experiences and abilities of organizations and artists who participated in the village mural project, but fundamentally, it is due to the projects of the government and local governments that focused on short-term visual tourism rather than long-term improvement of the residential environment. It was confirmed that the projects of the government and local governments were carried out through vertical integration and that there were problems in the actual work, such as requiring specific topics, subject matter, designs and colors. As a result, the village murals 'objectify' the residents and 'romanticized' the place, resulting in a unified visual representation. Therefore, this study suggests that the administration and accounting should be strictly managed, but in actual work, the vertical integration structure should be eased by simplifying various documents and procedures, as well as rich cultural understanding of the target place, selection of various topics, and original subject matter and expressions.

A Study on the Designing by the Personification Technique (의인화 기법으로 소구하는 디자인에 관한 연구)

  • Lee, Se Jung
    • Journal of the Korean Society of Floral Art and Design
    • /
    • no.42
    • /
    • pp.133-144
    • /
    • 2020
  • Anthropomorphism is one of the commonly used appeal methods in the field of communication design. Almost all visual images that humans encounter are exposed to this anthropomorphism, and we are consciously or unconsciously utilizing or being used. In addition, anthropomorphism can be found in almost all cultures and arts rather than in a specific field of study. Therefore, in this paper, the personification is redefined based on the relationship formation structure based on the anthropomorphic cases observed in culture and art and the results of previous studies. In addition, the personification form is defined as two kinds of personification and inverse personification according to the subject of relationship formation based on the personification type and gesture list derived from the previous study on the personification technique. Through the application cases of anthropomorphic techniques, which are appealed across the design domain, the effective anthropomorphic application system was defined. The definition of anthropomorphic relationship formation and anthropomorphic application system provided a framework for anthropomorphic techniques that could lead to effective audience satisfaction in various media. In addition, through the personification application system that synchronizes the characteristics of the conceptual traits of the medium with the gesture list and the personification type classification, it was confirmed that a device for communicating with the owner can be provided with a powerful and effective personification technique.