• IMMUNE NETWORK
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• v.14 no.2
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• pp.73-80
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• 2014

Because autoimmune diseases (AIDs) result from a complex combination of genetic and epigenetic factors, as well as an altered immune response to endogenous or exogenous antigens, systems biology approaches have been widely applied. The use of multi-omics approaches, including blood transcriptomics, genomics, epigenetics, proteomics, and metabolomics, not only allow for the discovery of a number of biomarkers but also will provide new directions for further translational AIDs applications. Systems biology approaches rely on high-throughput techniques with data analysis platforms that leverage the assessment of genes, proteins, metabolites, and network analysis of complex biologic or pathways implicated in specific AID conditions. To facilitate the discovery of validated and qualified biomarkers, better-coordinated multi-omics approaches and standardized translational research, in combination with the skills of biologists, clinicians, engineers, and bioinformaticians, are required.

• BMB Reports
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• v.37 no.1
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• pp.35-44
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• 2004

Recently produced information on post-translational modifications makes it possible to interpret their biological regulation with new insights. Various protein modifications finely tune the cellular functions of each protein. Understanding the relationship between post-translational modifications and functional changes ("post-translatomics") is another enormous project, not unlike the human genome project. Proteomics, combined with separation technology and mass spectrometry, makes it possible to dissect and characterize the individual parts of post-translational modifications and provide a systemic analysis. Systemic analysis of post-translational modifications in various signaling pathways has been applied to illustrate the kinetics of modifications. Availability will advance new technologies that improve sensitivity and peptide coverage. The progress of "post-translatomics", novel analytical technologies that are rapidly emerging, offer a great potential for determining the details of the modification sites.

• 대한두경부종양학회:학술대회논문집
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• 2004.11a
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• pp.210-210
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• 2004

• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.270-282
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• 2008

The yeast Saccharomyces cerevisiae has defense mechanisms identical to higher eukaryotes. It offers the potential for genome-wide experimental approaches owing to its smaller genome size and the availability of the complete sequence. It therefore represents an ideal eukaryotic model for studying cellular redox control and oxidative stress responses. S. cerevisiae Yap1 is a well-known transcription factor that is required for $H_2O_2$-dependent stress responses. Yap1 is involved in various signaling pathways in an oxidative stress response. The Gpx3 (Orp1/PHGpx3) protein is one of the factors related to these signaling pathways. It plays the role of a transducer that transfers the hydroperoxide signal to Yap1. In this study, using extensive proteomic and bioinformatics analyses, the function of the Gpx3 protein in an adaptive response against oxidative stress was investigated in wild-type, gpx3-deletion mutant, and gpx3-deletion mutant overexpressing Gpx3 protein strains. We identified 30 proteins that are related to the Gpx3-dependent oxidative stress responses and 17 proteins that are changed in a Gpx3-dependent manner regardless of oxidative stress. As expected, $H_2O_2$-responsive Gpx3-dependent proteins include a number of antioxidants related with cell rescue and defense. In addition, they contain a variety of proteins related to energy and carbohydrate metabolism, transcription, and protein fate. Based upon the experimental results, it is suggested that Gpx3-dependent stress adaptive response includes the regulation of genes related to the capacity to detoxify oxidants and repair oxidative stress-induced damages affected by Yap1 as well as metabolism and protein fate independent from Yap1.

• Earthquakes and Structures
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• v.26 no.6
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• pp.433-447
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• 2024

During earthquakes, regular buildings perform better than irregular buildings. In general, seismic design codes define a regular building using estimates of Storey Stiffness and Storey Strength. At present, seismic design codes do not recommend a specific method to estimate these parameters. Consequently, any method described in the literature can be applied to estimate the aforementioned parameters. Nevertheless, research has demonstrated that storey stiffness and storey strength vary depending on the estimation method employed. As a result, the same building can be regular or irregular, depending on the method employed to estimate storey stiffness and storey strength. Hence, there is a need to identify the best method to estimate storey stiffness and storey strength. For this purpose, the study presents a qualitative and quantitative evaluation of nine approaches used to determine storey stiffness. Similarly, the study compares six approaches for estimating storey strength. Subsequently, the study identifies the best method to estimate storey stiffness and storey strength using results of 350 linear time history analyses and 245 nonlinear time history analyses, respectively. Based on the comparison, it is concluded that the Fundamental Lateral Translational Mode Shape Method and Isolated Storey Method - A Particular Case are the best methods to estimate storey stiffness and storey strength of low-to-mid rise buildings, respectively.

• Journal of computational fluids engineering
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• v.8 no.4
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• pp.34-40
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• 2003

The effect of nose radius on aerodynamic heating is investigated by using the Navier-Stokes code extended to thermochemical nonequilibrium airflow, Spherical blunt bodies, whose nose radius varies from 0.O03048 m to 0.6096 m, flying at Mach 25 at an altitude of 53.34 km are considered. Comparison of heat flux at stagnation point with the solution of Viscous Shock Layer and Fay-Riddell are made. Results show that the flow for very small radius is in a nearly frozen state, and therefore the heat flux due to diffusion is smaller than that due to translational energy. As the radius becomes larger, the portion of heat flux by diffusion becomes greater than that of heat flux by translational temperature and approaches to a constant value.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.6
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• pp.1496-1502
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• 1994

The influences of spring position and spring stiffness on the critical force of a cantilevered beam subjected to a follower force are investigated. The spring attatched to the beam is assumed to be a translational one and can be located at arbitrary positions of the beam as it has not been assumed so far. The effects of transeverse shear deformation and rotary intertia of the beam are also included in this analysis. The charateristic equation for the system is derived and a finite element model of the beam using local coordinates is formulated through extended Hamilton's principle. It is found that when the spring is located at position less than that of 0.5L, the flutter type instability only exists. It is shown that the spring position approaches to the free end of the beam from its midpoint, instability type is changed from flutter to divergence through the jump phenomina according to the increase of spring stiffness.

• 한국전산유체공학회:학술대회논문집
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• 2002.05a
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• pp.109-114
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• 2002

The effect of nose radius on aerodynamic heating are investigated by using the Wavier-Stokes code extended to thermochemical nonequilibrium airflow. A spherical blunt body, whose radius varies from 0.003048 m to 0.6096 m, flying at Mach 25 at an altitude of 53.34 km is considered. Comparison of heat flux at stagnation point with the solution of Viscous Shock Layer and Fay-Riddell are made. Obtained result reveals that the flow chemistry for very small radius is nearly frozen, and therefore the contribution of heat flux due to chemical diffusion is smaller than that of translational energy. As the radius becomes larger, the portion of diffusion heat flux becomes greater than translational heat flux and approaches to a constant value.

• Journal of Periodontal and Implant Science
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• v.51 no.2
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• pp.100-113
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• 2021

Purpose: Previous studies have solely focused on fresh extraction sockets, whereas in clinical settings, alveolar sockets are commonly associated with chronic inflammation. Because the extent of tissue destruction varies depending on the origin and the severity of inflammation, infected alveolar sockets may display various configurations of their remaining soft and hard tissues following tooth extraction. The aim of this study was to classify infected alveolar sockets and to provide the appropriate treatment approaches. Methods: A proposed classification of extraction sockets with chronic inflammation was developed based upon the morphology of the bone defect and soft tissue at the time of tooth extraction. The prevalence of each type of the suggested classification was determined retrospectively in a cohort of patients who underwent, between 2011 and 2015, immediate bone grafting procedures (ridge preservation/augmentation) after tooth extractions at Seoul National University Dental Hospital. Results: The extraction sockets were classified into 5 types: type I, type II, type III, type IV (A & B), and type V. In this system, the severity of bone and soft tissue breakdown increases from type I to type V, while the reconstruction potential and treatment predictability decrease according to the same sequence of socket types. The retrospective screening of the included extraction sites revealed that most of the sockets assigned to ridge preservation displayed features of type IV (86.87%). Conclusions: The present article classified different types of commonly observed infected sockets based on diverse levels of ridge destruction. Type IV sockets, featuring an advanced breakdown of alveolar bone, appear to be more frequent than the other socket types.

• BMB Reports
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• v.47 no.8
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• pp.417-423
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• 2014

MicroRNAs (miRNAs) are a large family of post-transcriptional regulators, which are 21-24 nt in length and play a role in a wide variety of biological processes in eukaryotes. The past few years have seen rapid progress in our understanding of miRNA biogenesis and the mechanism of action, which commonly entails a combination of target degradation and translational repression. The target degradation mediated by Argonaute-catalyzed endonucleolytic cleavage exerts a significant repressive effect on target mRNA expression, particularly during rapid developmental transitions. This review outlines the current understanding of the mechanistic aspects of this important process and discusses several different experimental approaches to identify miRNA cleavage targets.