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Monitoring of Pesticide Residues in Green Tea produced in Korea (국내산 녹차의 잔류농약 모니터링)

  • Kim, Hee-Yun;Jeon, Jong-Sup;Kim, Yong-Hoon;Choi, Hee-Ju;Cheong, So-Young;Lee, Hwa-Jeong;Kim, Jae-In;Kim, Young-Seon;Choi, Gye-Sun;Choi, Jae-Chun
    • Korean Journal of Food Science and Technology
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    • v.41 no.5
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    • pp.483-489
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    • 2009
  • Herein, we present the results of our investigation of 61 pesticide residues in 100 samples of green teas obtained from Korean markets. Bifenthrin, chlorpyrifos, dicofol, EPN, chlorfenapyr, tebuconazole, cyhalothrin, difenoconazole, and tebufenpyrad were detected in 22 of the 100 green tea samples. The quantity of pesticide residues for bifenthrin was 0.12 ppm (maximum residue limits (MRLs): 0.3 ppm) in one sample, chlorpyrifos was 0.24-0.78 ppm (MRLs: 2.0 ppm) in three samples, dicofol was 1.64-4.19 ppm (MRLs: 50.0 ppm) in two samples, EPN was 0.13 ppm (MRLs: 0.05 ppm) in one sample, chlorfenapyr was 0.01-1.23 ppm (MRLs: 3.0 ppm) in 16 samples, tebuconazole was 0.71 ppm (MRLs: 5.0 ppm) in one sample, cyhalothrin was 0.05-0.3 ppm (MRLs: 2.0 ppm) in five samples, difenoconazole was 0.23 ppm (MRLs: 2.0 ppm) in one sample, and tebufenpyrad was 0.06-0.07 ppm (MRLs: 2.0 ppm) in two samples. More than two pesticide residues were detected in seven samples. All detected pesticide residues (with the exception of EPN) were within the MRLs.

Antrum Formation and Growth of Mouse Pre-antral Follicles Cultured in Two Different Culture Media without Hormones

  • Kim, Ju-Hwan;Kim, Hwan-Tae;Park, Kee-Sang;Song, Hai-Bum;Chun, Sang-Sik
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.8-8
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    • 2001
  • Mouse follicles require the addition of gonadotropins (Gns) to complete maturation and ovulation of oocyte and antrum formation of follicles in vitro. However, we tried examination of in vitro growth of mouse pre-antral follicles in medium without Gns and physiological factors. And also, pre-antral follicles were isolated from ovaries by mechanical method. Our present studies were conducted to evaluate on the growth of follicles and intra-follicular oocytes and antrum formation in vitro of mouse pre-antral follicles in two different media. Pre-antral follicles (91-120${\mu}{\textrm}{m}$) were isolated mechanically by fine 30G needles not using enzymes from ovary of 3-6 weeks old female ICR mice. Isolated pre-antral follicles were cultured in 20 ${mu}ell$ droplets of TCM (n=17; follicles: 107.8 $\pm$ 1.58 ${\mu}{\textrm}{m}$; oocytes: 59.9$\pm$1.2 ${\mu}{\textrm}{m}$) or MEM (n=12; follicles: 109.3$\pm$2.53 ${\mu}{\textrm}{m}$; oocytes: 55.4 $\pm$1.6${\mu}{\textrm}{m}$) under mineral oil on the 60mm culture dish. All experimental media was supplemented with 10% FBS but without Gns and/or physiological factors. Pre-antral follicles were individually cultured in drops for 8 days. Antrum formation and growth of pre-antral follicles and intra-follicular oocytes were evaluated using a precalibrated ocular micrometer at $\times$200 magnifications during in vitro culture. Results between different groups were analyzed using combination of Student's t-test and Chi-square, and considered statistically significant when P<0.05. Antrum formation of pre-antral follicles had started in two culture media on day-2. On day-8, antrum formation had occurred in 58.3%(7/12) of pre-antral follicles cultured in MEM, but only in 23.5% (4/17) of those cultured in TCM (P=0.0364). Growth of pre-antral follicles and intra-follicular oocytes were observed on day-4 and -8. On day-4, follicular diameters was similar (P=0.1338) in TCM (119.4$\pm$2.58 ${\mu}{\textrm}{m}$) and MEM (125.4$\pm$4.52 ${\mu}{\textrm}{m}$). However, on day-8, diameters of pre-antral follicles cultured in MEM (168.9$\pm$17.29 ${\mu}{\textrm}{m}$) was significantly (P=0.0248) bigger than that in TCM (126.7$\pm$4.28 ${\mu}{\textrm}{m}$). On day-4 and -8, diameters of intra-follicular oocytes were similar TCM (67.1$\pm$1.3 and 72.4$\pm$0.9${\mu}{\textrm}{m}$) and MEM (65.2$\pm$1.7 and 73.3$\pm$1.5 ${\mu}{\textrm}{m}$), respectively. We can conform that medium not supplemented with Gns and/or physiological factors can be used for in vitro antrum formation and growth of mouse pre-antral follicles and intra-follicular oocytes. In conclusion, MEM supplemented with FBS can be used for growth in vitro of mouse pre-antral follicles isolated mechanically.

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A Study on The Performance and Fuel Economy of Diesel Vehicles According to Change in Fuel Properties (연료물성에 따른 경유 차량의 성능 및 에너지소비효율 연구)

  • Noh, Kyeong-Ha;Lee, Min-Ho;Kim, Ki-Ho;Lee, Jung-Min
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.3
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    • pp.667-675
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    • 2018
  • Increasing emissions regulations and demand of high-efficiency cars that travels a lot of distance with less fuel, there is growing interest in Energy Consumption Efficiency. Korean energy consumption efficiency compute combined Fuel Economy by driven city & highway driving mode and present final Energy Consumption Efficiency as using 5-cycle correction formula. Energy consumption efficiency is computed Carbon-balance-method, when used burning fuel play a key role in vehicle performance & Energy Consumption Efficiency. In Korea, vehicle fuel is circulate by Petroleum and Petroleum Alternative Business Act, there is property difference in quality standard because petroleum sector's refine method or type of crude oil. It does not appear a big difference according to fuel, because it sets steady quality standard, it may affect the performance of automobile. Thus, in research We purchase a few diesel fuel which circulated in the market in summer season though directly-managed-gas station by petroleum sector, resolve property each of fuel, we compute Fuel Economy each of them. We analyze into change depend on applying for property as nowadays utilizing Energy Consumption Efficiency calculating formula of gasoline and diesel fuel. As result, Density each of sample fuel has a maximum difference roughly 0.9%, net heat value each of sample fuel has difference 1.6%, result of current Energy Consumption Efficiency each of sample fuel has a difference roughly 1% at city drive mode, 1.4% at highway drive mode. Result of use gasoline calculator formula shows less 6% result than nowadays utilizing Energy Consumption Efficiency calculating formula, each of sample's Energy Consumption Efficiency shows maximum roughly 1.4% result in city & highway drive mode.

Risk Assessment of Agricultural Worker's Exposure to Fungicide Thiophanate-methyl during Treatment in Green Pepper, Cucumber and Apple Fields (고추, 오이 및 사과 재배 중 살균제 Thiophanate-methyl 살포 시 농작업자의 노출 및 위해 평가)

  • Choi, Hoon;Kim, Jeong-Han
    • Journal of Applied Biological Chemistry
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    • v.57 no.1
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    • pp.73-81
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    • 2014
  • The present study was carried out to assess exposure and risk to thiophanate-methyl wettable powder for agricultural worker during mixing/loading and application with power sprayer in green pepper, cucumber and apple fields. Dermal exposure was measured with patches, gloves, socks and masks, while inhalation exposure was evaluated with personal air pump and solid sorbent. Those methods were full validated before experiment. During mixing/loading, dermal exposure amount in green pepper, cucumber and apple fields was $24.0{\pm}6.7$, $4.5{\pm}1.5$ and $18.5{\pm}0.6mg$, corresponding to mean 0.007, 0.001 and 0.005% of prepared active ingredient, respectively. The major exposed part for mixer/loader was hands (78-92%). Dermal exposure amount for applicator in green pepper, cucumber and apple fields was $84.9{\pm}14.0$, $34.0{\pm}20.8$ and $30.7{\pm}9.1mg$, corresponding to mean 0.024, 0.016 and 0.013% of applied active ingredient, respectively. The main body parts of exposure in apple field were hands, while thighs and shins in other fields. Inhalation exposure amount in green pepper, cucumber and apple fields was $1.5{\pm}2.2$, $52.7{\pm}48.9$ and $4.0{\pm}4.9{\mu}g$ during mixing/loading and $0.2{\pm}0.1$, $23.2{\pm}12.4$ and $0.4{\pm}0.6{\mu}g$ for applicator, respectively. These results were suggested that main factors affecting dermal exposure were contact frequency to the plants, foliage density, hygienic behavior, work type, and working environment, while inhalation exposure was affected mainly by working environment, especially wind. In risk assessment, margin of safety for thiophanate-methyl in all cases was over 1. However, during application in green pepper field, margin of safety was close to 1.

Effect of Small Dose of Radiation on Induction of Apoptosis in Murine Tumors (마우스 종양에서 저선량 방사선이 Apoptosis의 유도에 미치는 영향)

  • Seong, Jin-Sil;Pyo, Hong-Ryull;Chung, Eun-Ji;Kim, Sung-Hee;Suh, Chang-Ok
    • Radiation Oncology Journal
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    • v.17 no.4
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    • pp.307-313
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    • 1999
  • Purpose : To investigate the Presence of adaptive response by low dose radiation in murine tumors in relation to radiation induced apoptosis as well as related mechanism. Materials and Methods : Syngeneic murine tumors, OCa-I and HCa-l, were given 0.05 Gy pretreatment followed by therapeutic dose of 25 Gy radiation. Induction of apoptosis was analyzed for each treatment group. Regulating molecules of apoptosis, p53, Bcl-2, Bax, Bcl-X, were also analyzed by Western blotting. Results : In 0.05 Gy pretreatment group of OCa-I, 25 Gy-induced apoptosis per 1000 cells was 229, which was estimated at $30\%$ lower level than the expected (p<0.05). In contrast, this reduction in radiation induced apoptosis was not seen in HCa-l. In the expression of apoptosis regulating molecules, p53 increased in both tumors in response to radiation. Bcl-2 and Bax did not show significant change in both tumors however, the expression of Bcl-2 surpassed that of Bax in 0.05 Gy pretreatment group of OCa-l. Bcl-X was not expressed in OCa-l. In HCa-l, Bcl-X showed increased expression even with 0.05 Gy. Conclusion : Adaptive response by low dose radiation Is shown in one murine tumor, OCa-l, in relation to radiation induced apoptosis. Apoptosis regulating molecules including Bcl-2/Bax and Bcl-X, appear to related. This study shows an evidence that adaptive response is present, but not a generalized phenomenon in vivo.

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Effect of Dietary Fish Oil on Lipid Peroxidation and Antiperoxidative System in Rat Liver and Brain -Sex-related Differences- (어유(魚油)섭취가 흰쥐의 간과 뇌조직의 지질과산화물 형성과 항산화계에 미치는 영향 -성(性)의 차이를 중심으로-)

  • Choi, Kyung-Won;Park, Myungg-Hee;Chang, Kyung-Sook;Cho, Sung-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.16 no.2
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    • pp.147-155
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    • 1987
  • In order to examine the effect of dietary fish oil on lipid peroxide formation and antiperoxidative efficiency in liver and brain, a group of male and female rats weighing about 70 grams were fed for three months, diet containing mackerel oil(MO) at the level of 10% (w/w). Results were compared, according to sex and source of dietary fat, i.e., in addition to MO, perilla oil(PO), soybean oil(SO), rapeseed oil(RO) or beef tallow(BT). Liver lipid peroxide level was significantly higher and levels of ${\alpha}-tocopherol$ and reduced glutathione(GSH) were lower in MO group than in other groups. This phenomenon was less clear in male than in female. Liver GSH level was lower in male, compared to female, but oxidized glutathione (GSSG) level did not vary, depending on either sex or dietary fat source. Brain lipid peroxide and ${\alpha}-tocopherol$ levels were not different among five experimental groups. Activities of liver and brain glutathione peroxidase and superoxide dismutase were not changed by dietary fat source, but glutathione peroxidase activity was higher in female than in male. The present study shows (a) that there is sex-related difference in antiperoxidatiye activity and (b) that fish oil containing $C_{20-22}({\omega}3)$ fatty acids, increases body lipid peroxide level and consumes more of cellular antioxidant, although it has lower total PUFA content than perilla or soybean oils.

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Physicochemical Properties of Fish-meat Gels Prepared from Farmed-fish (해수어를 활용한 연제품의 제조 및 물리화학적 특성)

  • Kim, Hyung Kwang;Kim, Se Jong;Karadeniz, Fatih;Kwon, Myeong Sook;Bae, Min-Joo;Gao, Ya;Lee, Seul-Gi;Jang, Byeong Guen;Jung, Jun Mo;Kim, Seo yeon;Kong, Chang-Suk
    • Journal of Life Science
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    • v.25 no.11
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    • pp.1280-1289
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    • 2015
  • Fish-meat gel is being produced mostly relying on surimi and raw materials imported from Southeast Asia and North America and present in small amount in local markets. In this study, common farmed local fishes were examined as stable and reliable sources of surimi for fish-meat gel production. For testing, five main farmed-fish of Korea, namely; Bastard halibut (Paralichthys olivaceus), Red sea bream (Pagrus major), Korean rockfish (Sebastes schlegeli), Common mulle (Mugil cephalus), and Finespotted flounder (Pleuronichthys cornutus) were used following a traditional washing process. The quality of the surimi was determined by the values of water content, whiteness index, gel strength and impurity. Accordingly, fish-meat gel and surimi quality experiments were carried out by measuring compressive and texture properties, expressible moisture content, Hunter color scale values and SDS-page protein patterns. Also gel characteristics were compared with that of FA and RA grade surimi (Alaska Pollock). Fish-meat gels were prepared by salt mincing the farmed-fish surimi with NaCl (2% w/w) and moisture adjustment to 84% by ice water adding. Prepared fish-meat paste was filled into 20-25 cm long polyvinylidene chloride casings and heated at 90℃ for 20 min. The whiteness values of fish-meat gels produced from surimi were increased by using farmed-fish and became comparable to that of FA Alaska Pollock gel. Among all tested farmed-fish, P. olivaceus and P. major exhibited better properties than RA Alaska Pollock and similar properties to FA Alaska Pollock. Therefore, current data suggests that fish farming can be an efficient and sustainable fish-meat source for fish-meat gel production in Korea.

A Study on the Influence of Information Security on Consumer's Preference of Android and iOS based Smartphone (정보보안이 안드로이드와 iOS 기반 스마트폰 소비자 선호에 미치는 영향)

  • Park, Jong-jin;Choi, Min-kyong;Ahn, Jong-chang
    • Journal of Internet Computing and Services
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    • v.18 no.1
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    • pp.105-119
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    • 2017
  • Smartphone users hit over eighty-five percentage of Korean populations and personal private items and various information are stored in each user's smartphone. There are so many cases to propagate malicious codes or spywares for the purpose of catching illegally these kinds of information and earning pecuniary gains. Thus, need of information security is outstanding for using smartphone but also user's security perception is important. In this paper, we investigate about how information security affects smartphone operating system choices by users. For statistical analysis, the online survey with questionnaires for users of smartphones is conducted and effective 218 subjects are collected. We test hypotheses via communalities analysis using factor analysis, reliability analysis, independent sample t-test, and linear regression analysis by IBM SPSS statistical package. As a result, it is found that hardware environment influences on perceived ease of use. Brand power affects both perceived usefulness and perceived ease of use and degree of personal risk-accepting influences on perception of smartphone spy-ware risk. In addition, it is found that perceived usefulness, perceived ease of use, degree of personal risk-accepting, and spy-ware risk of smartphone influence significantly on intention to purchase smartphone. However, results of independent sample t-test for each operating system users of Android or iOS do not present statistically significant differences among two OS user groups. In addition, each result of OS user group testing for hypotheses is different from the results of total sample testing. These results can give important suggestions to organizations and managers related to smartphone ecology and contribute to the sphere of information systems (IS) study through a new perspective.

Influences of Feeding Seleniferous Whole Crop Barley Silage on Growth Performance and Blood Characteristics in Growing Hanwoo Steers (셀레늄함유 청보리 사일리지급여가 육성기 거세한우의 생산성 및 혈액성상에 미치는 영향)

  • Kim, Guk-Won;Jo, Ik-Hwan;HwangBo, Soon;Lee, Sung-Hoon;Han, Ouk-Kyu;Park, Tae-Il;Choi, In-Bae
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.2
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    • pp.139-148
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    • 2012
  • This study was conducted to investigate effects of feeding seleniferous whole crop barley silage (WCBS) on growth performance and blood characteristics in growing Hanwoo steers. A total of 20 growing Hanwoo steers, initially weighing on average 208.8 kg of body weight, were used and treatments were consisted of 1) controls : 0.1 mg/kg Se, 2) T1 : 0.3 mg/kg Se, 3) T2 : 0.9 mg/kg Se by combining seleniferous and/or non-seleniferous WCBS, and 4) T3 : 0.9 mg/kg Se of inorganic Se treatment. Five steers were allocated to each treatment, and the trial was lasted for 90 days. All experimental diets were included to 30% of combination of seleniferous and/or non-seleniferous WCBS, and in T3 diet, sodium selenite that corresponds to 0.9 mg/kg Se was added to control diets. Also, the diets were isonitrogenous and isocaloric among treatments. Dietary level and type of selenium did not affect feed intakes and daily gain, and blood glucose concentration was significantly (p<0.05) lower for controls than T1 and T2 treatments. Blood total lipid concentration was significantly (p<0.05) decreased with increasing levels of dietary selenium, and also that of T2 and T3 was significantly (p<0.05) lower than controls. LDL-cholesterol concentration was significantly (p<0.05) lower for treatments including dietary selenium than controls, and also blood triglyceride concentration was significantly (p<0.05) lower for T2 than controls. Overall, it was tended that feeding seleniferous WCBS or inorganic Se increased blood IgG concentration, and in the same dietary selenium levels, T2 treatment was higher for IgG than T3 group (p>0.05). Blood selenium concentration was significantly (p<0.05) increased by feeding increasing levels of seleniferous WCBS, but there was no significant difference between controls and T3 group. These results showed that feeding seleniferous WCBS to growing Hanwoo steers was responsible for saccharide and lipid metabolism, and in particular, it reduced their total lipid and blood LDL-cholesterol concentrations. Furthermore, selenium present in seleniferous WCBS rather than inorganic selenium was better available in intestinal absorption, and it might help to enhance immunity in growing and younger stages of Hanwoo steers.

Study on the Estimation of Selection Index in Broiler Breeder I. Estimation of Genetic Parameters in Broiler (육용종계의 선발지수 추정에 관한 연구 I. 육용종계 부계통과 모계통의 유전적 모교추정)

  • 김기경;손시환;오봉국
    • Korean Journal of Poultry Science
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    • v.11 no.2
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    • pp.86-92
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    • 1984
  • Present study was carried out to estimate phenotypic and genetic parameters influencing body weight (BW) at 4 weeks of age egg breadth (EB), egg length(EL), egg shape index (SI) and egg weight (EW) at 32 weeks of age and egg numbers (EN) up to 38 weeks of age in broiler male and female lines. The data were collected from closed White Plymouth Rock (female line; G) and Cornish (male line; C) flocks involving 1193 pullets from 211 dams and 48 sires in 1982. The results obtained are summarized as follow: 1. General performance for various trails of lines C and G. The means and standard deviations of BW, EB, EL, SI, EW and EN were 668.34${\pm}$47.18, 4.23${\pm}$0,11, 5.49 ${\pm}$0.19, 77.06${\pm}$2.98, 55.73${\pm}$3.54 and 59.72${\pm}$13.39 in line C, respectively and 487.89${\pm}$ 41.43, 4.22${\pm}$0.11, 5.51${\pm}$0.19, 76.72${\pm}$3.20, 55.43${\pm}$3.26 and 76.93${\pm}$12.17 in line G, respectively. 2. Heritability Heritabilities were estimated from sire, dam and combined components. Estimates for BW, EB, EL, SI, EW and EN from combined components were 0.30, 0.29, 0,40, 0.22, 0.45 and 0.60 in line C, respectively and 0.33, 0.23, 0.28, 0.13, 0.49 and. 0.33 in line G, respectively. 3. Correlation Genetic and phenotypic correlations showed similar trend in line C and G. Genetic correlations, estimated EW with EB and EL, were high and positive (line C; 0.99, 0.75, respectively and line G; 0.94, 0.82, respectively), also correlation of EB with EL was 0.58 (both lines; 0.58). High and negative genetic correlations were shown between SI and EL in line C and G (-0.70, -0.65, respectively). Genetic correlations between SI and EW were relatively low and negative in line C and G (-0.11, -0.19, respectively) and between SI and EN were relatively low and positive in line C and G (0.25, 0.17, respectively). Between other traits, low genetic correlations were shown in both lines, High and positive correlation was estimated between hatchability and egg shape index and polynomial regression of egg shape index on hatchability was estimated; Y=-216.77+7.6216X-0.0146939X$^2$.

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