• 한국콘텐츠학회논문지
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• 제9권5호
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• pp.206-213
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• 2009

본 연구는 배변조영촬영 시 피폭을 받는 생식선(난소, 고환)에 대한 선량과 필터사용에 따른 선량의 차이를 측정하여 유용성을 분석한 것으로 그 결과는 다음과 같다. 난소와 고환이 받는 선량은 필터를 사용하지 않은 경우에 왼쪽 난소(Left Ovary) 23.4mGY, 오른쪽 난소(Right Ovary) 7.5mGy, 고환(Testis) 10.3mGy이었고, 부가필터만 사용한 경우 왼쪽 난소 22.4mGy, 오른쪽 난소 7.0mGy, 고환 9.5mGy이었다. 또한, 배변조영용 필터만 사용한 경우 왼쪽 난소 26.7mGY, 오른쪽 난소 8.4mGY, 고환 11.5mGy로 나타났으며, 두 가지 필터를 모두 사용한 경우 왼쪽 난소 20.5mGy, 오른쪽 난소 5.2mGy, 고환 7.5mGy이었다. 양측 난소와 고환 모두 필터의 사용에 따라 선량의 차이가 유의성이 있는 것으로 나타났다. 이는 필터의 사용 조합에 따라 필터를 사용하지 않았을 때를 기준으로 부가필터만 사용했을 때 왼쪽 난소는 선량이 10% 감소되었고, 오른쪽 난소 5%, 고환 8% 감소하였다. 배변조영용 필터만을 사용했을 때는 오히려 왼쪽 난소가 33% 선량이 증가하였고, 오른쪽 난소 9%, 고환은 12% 증가하였다. 두 가지 필터를 모두 사용하였을 때에는 왼쪽 난소는 2994 감소되었고, 오른쪽 난소 13%, 고환은 28% 감소하였다. 이와 같은 결과는 배변조명용 필터만을 사용했을 때는 오히려 선량이 증가하였으며 부가필터만 사용하거나 두 가지 필터 모두 사용하였을 때 현저히 선량이 감소하는 것으로 나타났다.

• 한국동물학회지
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• 제29권3호
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• pp.190-200
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• 1986

논우렁이의 組織別 蛋白質 패턴에 있어서 筋肉은 雌雄에 關係없이 16個의 主要蛋白質 bands는 거의 同一하였으며, 그 중에서 bands E (Mr. 41,500), H (Mr. 52,100), L (Mr. 71,700), N(Mr. 98,500), O (Mr. 107,900) 와 P (Mr. 112,900)는 筋肉과 精巢 卵巢에서 나타나는 蛋白質이었다. 그러나 精巢나 卵巢의 蛋白質 패턴은 그들 固有의 蛋白質 패턴을 가지고 있었으며 組織에서 全部 나타나는 band外에 精巢와 卵巢에서 共同으로 나타나는 主要한 bands는 약 5個[bands d (Mr. 15,600), k (Mr. 37,100), p (Mr. 57,000), s (Mr. 80,300), v (Mr. 105,400)]로 나타남을 알 수 있었다. 그리고 主要蛋白質 bands를 elution 시켜서 아미노산을 分析한 結果는 단백질 패턴의 양상과 같이, 아미노산의 量과 組成이 많은 순서는 卵巢, 筋肉, 精巢의 順으로 되었다. 이러한 結果를 볼 때 卵巢에서는 全個體에서 가질수 있는 豊富한 蛋白質이 發見되는 同時에 精巢에서는 核酸物質의 濃縮으로 情蟲이 되기 위한 morphogenesis의 現象으로 말미 암아 蛋白質의 量이 매우 적게 나타나는 것으로 生覺되었다.

• Journal of Nutrition and Health
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• 제3권2호
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• pp.101-106
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• 1970

Radioactive Phosphorus $(^{32}P)$ was administered intramuscularlly to the newly hatched chicken in the purpose of determination of the uptake and the distribution, as related to sex and hour differences of the several organs of the bodies. $2\;{\mu}\;of\;^{32}P$ was administered to each chick, and the distribution of 32P was observed in 1 hour and 24 hours after administration. In this experiment 80 heads of chicken were used(40 chicken were one day and 40 chicken were 7 days old) and the results obtained as follows: 1. The tissue showed an uptake rate of $^{32}P$ dose per 100 milligram of tissue in one day old chicken, with the following sequence: Males (1 hour): Femur. Liver. G., Muscle. Testis. Brain (24 hour): Femur, Testis, Gastrocnemius Muscle, Liver, Brain. Female(1 hour): Femur, Liver, Gastronemius Muscle, Ovary, Brain. (24 hour): Femur, Liver, Gastrocnemius Muscle, Ovary, Brain. 2. In 1 hour, the uptake rate of $^{32}P$ of the tissues showed significant difference between the male and the female except the gastrocnemius muscle and the brain in one day old group, but they were no significance except the testis and ovary after 24 hours. 3. The distribution of $^{32}P$ of the tissues exhibited higher in 1 hour than in 24 hours except the femur, the brain of the male and female, the brain and gastrocnemius muscle of the female in one day old group. 4. The tissue showed an uptake rate of $^{32}P$ dose per 100 miligram of tissue in 7days old chicken, with the following sequence: Male (1 hour): femur, liver, gastrocmenius muscle, testis, brain. (24 hour): femur, testis, gastrocmenius muscle, liver, brain. Female(1 hour): femur, liver, gastrocmenius muscle, ovary, brain. (24 hour): femur, ovary, liver, gastrocmenius muscle, brain. 5. The distribution of $^{32}P$ of the tissues showed no significant difference between the male and the female except the testis and ovary after 24 hours in 7 days old chicken group. 6. The distribution of $^{32}P$ the tissues exhibited higher in 1 hour in 24 hours except the femur, the brain of the male and the female, the brain and the ovary of the female in 7 days old chicken group.

• Genomics & Informatics
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• 제19권1호
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• pp.8.1-8.12
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• 2021

Cytorace-3 is a laboratory evolved hybrid lineage of Drosophila nasuta nasuta males and Drosophila nasuta albomicans females currently passing ~850 generations. To assess interracial hybridization effects on gene expression in Cytorace-3 we profiled the transcriptomes of mature ovaries and testes by employing Illumina sequencing technology and de novo transcriptome assembling strategies. We found 26% of the ovarian, and 14% of testis genes to be differentially expressed in Cytorace-3 relative to the expressed genes in the parental gonadal transcriptomes. About 5% of genes exhibited additive gene expression pattern in the ovary and 3% in the testis, while the remaining genes were misexpressed in Cytorace-3. Nearly 772 of these misexpressed genes in the ovary and 413 in the testis were either over-or under-dominant. Genes following D. n. nasuta dominance was twice (270 genes) than D. n. albomicans dominance (133 genes) in the ovary. In contrast, only 105 genes showed D. n. nasuta dominance and 207 showed D. n. albomicans dominance in testis transcriptome. Of the six expression inheritance patterns, conserved inheritance pattern was predominant for both ovary (73%) and testis (85%) in Cytorace-3. This study is the first to provide an overview of the expression divergence and inheritance patterns of the transcriptomes in an independently evolving distinct hybrid lineage of Drosophila. This recorded expression divergence in Cytorace-3 surpasses that between parental lineages illustrating the strong impact of hybridization driving rapid gene expression changes.

• 한국임상수의학회지
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• 제25권3호
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• pp.221-223
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• 2008

SRY gene is normally responsible for testis induction, yet testis development can occur in the absence of SRY. In here, we analyzed the SRY-negative sex reversal in cocker spaniel, at 1.5 year-old. The attacked dog was suffered from enlarged clitoris, and resulted in disorder of urination. By surgically approach, enlarged clitoris and one testis, which are apparently seen, are removed. Additionally, thorough the abdomen surgery, uterus and ovary-like mass were removed. The dog had XX, chromosome, showed negative for SRY-gene, and the mass had the ovary-testis structure. In other words, based on the macroscopic, cytogenic, and histological study, we can diagnose the cocker spaniel as SRY-negative sex reversal.

• Animal cells and systems
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• 제13권4호
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• pp.439-445
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• 2009

Cytochrome P450 aromatase (P450arom) catalyzes the conversion of androgens to estrogens and plays an important role in reproduction and development in vertebrates. We investigated the expression patterns of ovarian P450arom (P450aromA) and brain P450arom (P450aromB) mRNA during sex change in black porgy. Maturity was divided into seven stages from male to female (immature testis, mature testis, testicular portion of mostly testis, ovarian portion of mostly testis, testicular portion of mostly ovary, ovarian portion of mostly ovary, and mature ovary). P450aromA expression was significantly higher in the ovarian portion of mostly-ovarian stage fish, and P450aromB expression was highest in the brain of black porgy with mostly-ovarian gonads. Histology showed that testicular tissues were disintegrated with the development of ovarian tissue associated with an increase in the expression of the two P450arom mRNAs during sex change. Interestingly, among various tissues, P450aromA was only expressed in the ovary, and P450aromB was only expressed in the brain. To understand the role of gonadotropin-releasing hormone (GnRH) and estradiol ($E_2$), we injected exogenous hormone (GnRH analogue [GnRHa] and $E_2$) into immature black porgy. In the GnRHa group, expression of the two P450arom genes decreased 12 h after injection, and expression of the two P450arom genes were significantly higher at 6 dafter $E_2$ injection. These results provide useful baseline knowledge on the mechanism of natural sex change in black porgy.

• Asian-Australasian Journal of Animal Sciences
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• 제23권8호
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• pp.1000-1006
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• 2010

Phosducin (PDC) is a photoreceptor cell-specific protein that is phosphorylated by cyclic nucleotide-dependent protein kinase. PDC and PDC-like proteins (PDCL, PDCL2, and PDCL3) are members of a conserved family of small thioredoxin-like proteins that modulate the ${\beta}$- and ${\gamma}$-subunits of G-proteins. In mammals, Pdc, Pdcl, and Pdcl3 genes show ubiquitous expression; however, Pdcl2 gene expression is limited to the testis and ovary. The aim of the present study was to examine the expression patterns of chicken Pdcl2 (cPdcl2) during testicular and ovarian development. Protein sequence comparisons performed using the CLUSTAL X program revealed that the amino acid sequences and potential phosphorylation sites of cPDCL2 and mammalian PDCL2 proteins were highly conserved. Quantitative real-time PCR analysis revealed that cPdcl2 was differentially expressed in the testis and ovary. Specifically, cPdcl2 expression was detected at low levels in the ovary at all time points. In the testis, cPdcl2 expression was detected at low levels until 5 weeks of age. At 8 weeks of age, however, cPdcl2 showed increased expression levels in the testis. Using in situ hybridization, we detected high levels of cPdcl2 expression in the testis, particularly in the spermatocytes and round spermatids. In summary, our data describe expression patterns of germ cell-specific Pdcl2 during testicular and ovarian development in chickens.

• 한국발생생물학회지:발생과생식
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• 제25권4호
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• pp.299-303
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• 2021

The ascidian Halocynthia aurantium (sea peach), a marine invertebrate, belongs to the same genus of the phylum Chordata along with the ascidian Halocynthia roretzi (sea pineapple), which is one of the model animals in the field of developmental biology. The characteristics of development and reproduction of H. aurantium are not yet known in detail. In order to find out the spawning period of H. aurantium, we investigated development of the gonads during the annual reproductive cycle. Testis and ovary were both in the bisexual gonads (ovotestes) of H. aurantium, which is a hermaphrodite like H. roretzi. In H. aurantium, the right gonad was longer and slightly larger than the left gonad throughout the year. In each gonad, the number of the testis gonoducts was slightly higher than that of the ovary gonoducts. These features were similarly observed in H. roretzi. However, the number of the testis gonoducts and the ovary gonoducts in each gonad of H. aurantium was about half that of H. roretzi. The gonads of H. aurantium contracted during the winter and summer seasons. The gonads decreased to the smallest size around February, and then started to increase again in March. The gonads were most developed in September of the year. Therefore, it is estimated that the spawning of H. aurantium begins around this period.

• 대한방사선기술학회지:방사선기술과학
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• 제43권1호
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• pp.29-34
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• 2020

Mitochondria was observed much around the nuclear membrane of liver tissue where the energy metabolism process is active. Testis tissue had a large number of undifferentiated cells, and cristae in Inner membrane of Mitochondria was not observed clearly. Morphological damage occurred first in Inner membrane rather than the outer membrane. The kidney tissue was clearly observed in the form of cristae. Radiation-induced damage occurred at the edges of both ends, and the membrane was observed bursting with the thickness of the outer membrane. Small intestine cells were observed in many mitochondria in the tissues around the villus, where bowel movements were active. Morphological damage occurred with the outer and inner membranes getting tangled. Mitochondria sensitivity to radiation was sensitized in testis and small intestine tissues, and kidney, ovary and liver tissues were found to be resistant.

• Clinical and Experimental Reproductive Medicine
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• 제9권1_2호
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• pp.79-93
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• 1982

We collected a total of 109 patients with intersex during the past 16 years (1966-1982). They were summerized as follows: Klinefelter's syndrome, the most common disease, was found in 76 cases, Turner's syndrome in 3 cases, true hermaphroditism in 5 cases, male pseudohermaphroditism in 5 cases, female pseudohermaphroditism in 13 cases and others in 7 cases in which 2 cases of XX male syndrome, 1 case of agonadism, 1 case of hernia uteri inguinale and 3 cases of unclassified intersex were included. 2 mosaic Klinefelter's syndrome showed 46/47 XX/XXY and 1 mosaic Turner's syndrome showed 45/46 XO/XX. The 5 patients with true hermaphroditism included 2 cases that had an ovary on one side and a testis on the other, 1 case, seperate ovary and testis on each side, 1 case, an ovary on one side and a seperate testis and ovary on the other and 1 case, an ovary on one side and an ovotestis on the other. Sex chromosome study on the true hermaphroditism revealed 46 XX in 2 patients and 46/46 XX/XY mosaicism in 3 patients. In male pseudohermaphroditism, all patients had a short and blind vagina. Of which, familial tendency was found in 1 case. Her sister had operation for sex reversal for female. In female psedohermaphroditism, all the patients were adrenogenital syndrome. Operations for clitoridectomy and vaginoplasty were performed on 10 patients. Hydrocortisone was given to 6 patients. Menstruation started to occur 6 months and 4 months after the medical therapy respectively in 2 cases.