• BMB Reports
• /
• 제35권2호
• /
• pp.228-235
• /
• 2002

Methionine aminopeptidase (MetAP) catalyzes the removal of an amino-terminal methionine from a newly synthesized polypeptide. The enzyme was purified to homogeneity from Bacillus stearothermophilus (KCTC 1752) by a procedure that involves heat precipitation and four sequential chromatographs (including DEAE-Sepharose ion exchange, hydroxylapatite, Ultrogel AcA 54 gel filtration, and Reactive red 120 dye affinity chromatography). The apparent molecular masses of the enzyme were 81,300 Da and 41,000 Da, as determined by gel filtration chromatography and sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), respectively. This indicates that the enzyme is comprised of two identical subunits. The MetAP specifically hydrolyzed the N-terminal residue of Met-Ala-Ser that was used as a substrate, and exhibited a strong preference for Met-Ala-Ser over Leu-Gly-Gly, Leu-Ser-Phe, and Leu-Leu-Tyr. The enzyme has an optimal pH at 8.0, an optimal temperature at $80^{\circ}C$, and pI at 4.1. The enzyme was heat-stable, as its activity remained unaltered when incubated at $80^{\circ}C$ for 45 min. The Km and Vmax values of the enzyme were 3.0mM and 1.7 mmol/min/mg, respectively. The B. stearothernmophilus MetAP was completely inactivated by EDTA and required $Co^{2+}$ ion(s) for activation, suggesting the metal dependence of this enzyme.

• Journal of Acupuncture Research
• /
• 제22권2호
• /
• pp.211-217
• /
• 2005

Objectives : Terminal stage cancer patient from primary hepatocellular carcinoma metastasized into lungs was administered with cultivated wild ginseng herbal acupuncture for 5 months and observed progression. Methods : Cultivated wild ginseng herbal acupuncture was administered 5 times a week at about 150cc dosage per week. Dynamic CT was taken and interpreted at a university hospital. Results : Above patient was diagnosed with hepatocellular carcinoma and received one procedure of lobectomy and three procedures of TACE, but because of metastasis, chance of improvement was very obscure. Intensive treatment of cultivated wild ginseng herbal acupuncture five times a week for five months in association with moxibustion was done on the patient. Near elimination of the cancer cells metastasized into lungs were confirmed in terms of radiological impression through dynamic CT. Conclusion: From the results obtained in this study, cultivated wild ginseng herbal acupuncture can be an effective measure against terminal stage cancer. But this is a single case study and lack of extensive follow-up must be compensated by further researches.

• 대한의용생체공학회:의공학회지
• /
• 제20권1호
• /
• pp.75-80
• /
• 1999

홀터 심전계는 일반 심전계와 달리 활동중의 심전도를 기록하므로서 복잡한 심장질환을 효과적으로 모니터링할 수 있으나 24시간 동안의 심전도를 수집하여 진단하기 때문에 급작스런 심장질환에 대해서는 대처할 수 없다. 따라서 본 논문에서는 홀터 심전계와 같은 이동형 심전도 단말기에 900Mhz 대역을 사용하는 부선 데이터 통신망 인터페이스를 첨가하여 심장 이상으로 인한 급사위험이 있는 환자를 감시${\cdot}$관리할 수 있는 보행형 감시 시스템 모델을 제안하고 이동형 심전도 단말기와 담당 의사를 위한 휴대형 단말기를 구현함으로써 무선 데이터 통신망을 이용한 보행형 감시 시스템이 구현될 수 있음을 검증하였다.

• 한국정보통신학회논문지
• /
• 제11권11호
• /
• pp.2027-2031
• /
• 2007

최근 이동 통신 기술의 발달과 이동 단말의 보급률이 증가하면서, 사용자들은 휴대 및 이동 시에 빠르고 끊김없는 서비스를 요구하게 되었다. 이러한 요구 사항을 충족시키기 위해 IETF에서는 FMIPv6(Fast Handoff for Mobile IPv6)를 제안하였다. FMIPv6의 핸드오버 과정은 크게 이동 감지, 새로운 CoA 설정, 바인딩 갱신 과정으로 나뉜다. 하지만, 각각의 과정에서 지연이 생기고, CoA 설정 과정의 DAD(Duplicate Address Detection) 실행 시 큰 지연이 생긴다. 본 논문에서는 DAD 절차를 생략하고 AR(Access Router)에 이동 단말의 CoA에 관련된 정보를 저장하여 핸드오버 시 지연을 줄이는 방안을 제안한다.

• 한국통신학회논문지
• /
• 제12권1호
• /
• pp.71-81
• /
• 1987

ISDN의 효율적 활용을 위해서는 다양한 가입자 서어비스를 제공할 수 있는 terminal의 개발이 선결되어야 한다. 본 논문의 ISDN가입자-망 간 접속에 관한 2편의 논문중 제2부로서 ISDN가입자 단말장치의 일반적 관련요소에 대한 연구를 수행하였으며 ISDN이 제공할 수 있는 서어비스 중 가장 간단한 예로 전화 서어비스를 위한 stimulus mode type의 digital telephone을 설계, 제작하여 그 성능을 고찰하여 보았다. 본 digital telephone은 ECM(Echo Cancellation Method)방식을 사용하여 network에 접속되며 user-network간 신호방식으로 CCITT의 I.440-441에서 권고하는 LAPD Protocol을 채택하였고 소규모 ISDN교환 emulator와의 연동을 통하여 관련된 S/W 및 H/W개발의 타당성을 검증하였다. 또한 digital telephone으로서의 기본 기능인 음성의 디지틀화, man-machine interface등이 실현되었다.

• Journal of Microbiology and Biotechnology
• /
• 제18권4호
• /
• pp.624-630
• /
• 2008

Terminal restriction fragment length polymorphism (T-RFLP) is a method that has been frequently used to survey the microbial diversity of environmental samples and to monitor changes in microbial communities. T-RFLP is a highly sensitive and reproducible procedure that combines a PCR with a labeled primer, restriction digestion of the amplified DNA, and separation of the terminal restriction fragment (T-RF). The reliable identification of T-RF requires the information of nucleotide sequences as well as the size of T-RF. However, it is difficult to obtain the information of nucleotide sequences because the T-RFs are fragmented and lack a priming site of 3'-end for efficient cloning and sequence analysis. Here, we improved on the T-RFLP method in order to analyze the nucleotide sequences of the distinct T-RFs. The first method is to selectively amplify the portion of T-RF ligated with specific oligonucleotide adapters. In the second method, the termini of T-RFs were tailed with deoxynucleotides using terminal deoxynucleotidyl transferase (TdT) and amplified by a second round of PCR. The major T-RFs generated from reference strains and from T-RFLP profiles of activated sludge samples were efficiently isolated and identified by using two modified T-RFLP methods. These methods are less time consuming and labor-intensive when compared with other methods. The T-RFLP method using TdT has the advantages of being a simple process and having no limit of restriction enzymes. Our results suggest that these methods could be useful tools for the taxonomic interpretation of T-RFs.

• The Korean Journal of Pain
• /
• 제18권1호
• /
• pp.19-22
• /
• 2005

Background: Most terminal cancer patients suffered from intractable pain. For the treatment of these patients, opioids, via various routes, are usually administered. Continuous epidural opioid, especially morphine, administration is a good method for the management of intractable cancer pain. Methods: We retrospectively analyzed 347 terminal cancer patients, who had been treated with continuous epidural morphine infusion, between 1999 and 2004. For the epidural infusion, an epidural catheter was inserted, tunneled subcutaneously and exited from the anterior chest or abdomen. Multiday $Infursor^{(R)}$ (Baxter, 0.5 ml/h) was used for the continuous infusion. Results: Of the 347 patients studied, there were 211 males and 136 females. The mean treatment time was 54.7 days, ranging from 5 to 481 days. The mean starting and termination doses of morphine were 32.4 (for 5 days) and 100.0 mg, respectively. The doubling time of the morphine dose was 26.3 days, corresponded to a 3.8 percent increase per day. Incidental catheter removal was the most common side effect, which occurred 130 times in 61 cases. Conclusions: The procedure of epidural catheterization, with subcutaneous tunneling, was simple and inexpensive. Despite the disadvantages, such as incidental catheter removal, it is a useful method for the control of terminal cancer pain.

• 디지털융복합연구
• /
• 제12권11호
• /
• pp.451-459
• /
• 2014

본 연구는 정상 성인의 팔 흔들기 종류에 따른 보행 시 운동역학적인 변화를 알아보기 위해서 실시하였다. 정상 성인 대학생 45명을 대상으로 정상 팔 흔들기, 한 팔 흔들기, 두 팔 움직임 없음 보행, 인위적인 팔 흔들기의 4가지 팔 흔들기 종류에 따라 실험을 진행하였다. 이들의 운동역학적인 변화를 알아보기 위해 3차원적 관절 운동역학적 변화 검사인 6-camera Vicon MX motion analysis system을 통해 보행 변수를 측정하였다. 팔 흔들기에 따른 운동역학적인 매개 변수들인 각 관절에서 보행속도, 엉덩관절 일률, 말기 디딤기의 수직 지면반발력에서 유의한 차이를 보였다(p<.05). 이러한 연구결과는 팔 흔들기 종류에 따라서 정상인과 환자들의 치료와 평가를 위한 지침으로서 임상에서 사용할 수 있을 것으로 생각된다.

• Journal of Hospice and Palliative Care
• /
• 제8권2호
• /
• pp.200-208
• /
• 2005

목적: 악성 위장관 폐색 환자에서 완화적 시술이나 수술은 폐색의 다양한 증상을 조절할 뿐만 아니라 삶의 질을 향상시킨다. 본 연구는 완화적인 시술을 받았던 악성 위장관 폐색 말기암 환자의 임상적 특징과 예후인자를 알아보고자 하였다. 방법: 2002년 5월부터 2005년 5월까지 본원에서 악성 위장관 폐색으로 진단받아 완화적인 시술을 받았던 48명의 말기암 환자를 대상으로 후향적으로 조사하였다. 완화적인 암절제 환자는 제외하였다. 임상적 특성과 시술내용을 조사하였고 예후인자는 log-rank test를 이용한 단변량 분석을 하고 통계적으로 의미 있는 인자는 Cox's proportional hazard model을 사용하여 다변량 분석을 하였다. 결과: 연령의 중앙값은 65세이고 남자가 25명(52%), 여자가 23명(48%)이었다. 가장 많은 암은 대장직장암으로 26명(55%)이고 다음으로 10명(21%)의 위암이었다. 치료를 전혀 받지 않았던 환자는 25명(58%)이었고 20명(42%)은 치료를 받았으며 이 중 18명은 항암 치료를 받은 과거력이 있었다. 가장 흔한 증상은 통증으로 15명(31%)이었다. 활동도 1점 혹은 2점이 23명(48%), 3점 혹은 4점이 25명(52%)이었다. 가장 많은 완화적인 시술은 대장루술로 19명이 받았다. 완화적 시술로 인한 사망은 없었다. 단변량과 다변량 분석에 의해서 전체 생존기간과 무증상 생존기간에 대하여 활동도 만이 의미있는 독립 예후인자였다. 전체 중간 생존기간은 150일이었으며 무증상 중간 생존기간은 90일이었다. 결론: 완화적 시술을 받은 악성 위장관 폐색 환자의 전체 중간 생존기간과 무증상 중간 생존기간에 대하여 활동도만이 유일한 독립 예후인자였다.

• 대한정형도수물리치료학회지
• /
• 제11권2호
• /
• pp.62-70
• /
• 2005

The determination of peripheral nerve conduction velocity is an important part to electrodiagnosis. Its value as neurophysiologic investigative procedure has been known for many years but normal value of median and ulnar motor nerve was poorly reported in Korea. To evaluate of median and ulnar motor nerve terminal latency, amplitude of CMAP(compound muscle action potential), conduction velocity and F-wave latency for obtain clinically useful reference value. 71 normal volunteers(age, 19-65 years; 142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrine disorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking II was use for detected terminal latency, amplitude of CMAP, conduction velocity and F-wave latency of median and ulnar motor nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation, independent t-test was used to compare between Rt and Lt side also compare between different in genders. The results are summarized as follows: 1. Median motor nerve terminal latency was right 3.00ms, left 2.99ms and there was no significantly differences between right and left side and genders. 2. Median motor nerve amplitude of CMAP was right 17.26mV, left 1750mV and there was no significantly differences between right and left side and genders. 3. Median motor nerve conduction velocity was right 57.89m/sec, left 58.03m/sec and there was no significantly differences between right and left side and genders. 4. Median motor nerve F-wave latency was right 25.74ms, left 25.59ms and there was significantly differences between genders. 5. Ulnar motor nerve terminal latency was right 2.38ms, left 2.45ms and there was significantly differences between right and left side. 6. Ulnar motor nerve amplitude of CMAP was right 15.99mV, left 16.02mV and there was no significantly differences between right and left side and genders. 7. Ulnar motor nerve conduction velocity was right 60.35m/sec, left 59.73m/sec and there was no significantly differences between right and left side and genders. 8. Ulnar motor nerve F-wave latency was right 25.53ms, left 25.57ms and there was significantly differences between genders.