• Animal Bioscience
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• v.34 no.10
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• pp.1579-1589
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• 2021

Objective: This study was conducted to generate single stranded DNA oligonucleotides with selective affinity to bovine spermatozoa, assess its binding potential and explore its potential utility in trapping spermatozoa from suspensions. Methods: A combinatorial library of 94 mer long oligonucleotide was used for systematic evolution of ligands by exponential enrichment (SELEX) with bovine spermatozoa. The amplicons from sixth and seventh rounds of SELEX were sequenced, and the reads were clustered employing cluster database at high identity with tolerance (CD-HIT) and FASTAptamer. The enriched nucleotides were predicted for secondary structures by Mfold, motifs by Multiple Em for Motif Elicitation and 5' labelled with biotin/6-FAM to determine the binding potential and binding pattern. Results: We generated 14.1 and 17.7 million reads from sixth and seventh rounds of SELEX respectively to bovine spermatozoa. The CD-HIT clustered 78,098 and 21,196 reads in the top ten clusters and FASTAptamer identified 2,195 and 4,405 unique sequences in the top three clusters from the sixth and seventh rounds, respectively. The identified oligonucleotides formed secondary structures with delta G values between -1.17 to -26.18 kcal/mol indicating varied stability. Confocal imaging with the oligonucleotides from the seventh round revealed different patterns of binding to bovine spermatozoa (fluorescence of the whole head, spot of fluorescence in head and mid- piece and tail). Use of a 5'-biotin tagged oligonucleotide from the sixth round at 100 pmol with 4×10⁶ spermatozoa could trap almost 80% from the suspension. Conclusion: The binding patterns and ability of the identified oligonucleotides confirms successful optimization of the SELEX process and generation of aptamers to bovine spermatozoa. These oligonucleotides provide a quick approach for selective capture of spermatozoa from complex samples. Future SELEX rounds with X- or Y- enriched sperm suspension will be used to generate oligonucleotides that bind to spermatozoa of a specific sex type.

• Journal of Microbiology and Biotechnology
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• v.22 no.3
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• pp.301-310
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• 2012

Prosopis juliflora and Parthenium hysterophorus are the two arid, exotic weeds of India that are characterized by distinct, profuse growth even in nutritionally poor soils and environmentally stressed conditions. Owing to the exceptional growth nature of these two plants, they are believed to harbor some novel bacterial communities with wide adaptability in their rhizosphere. Hence, in the present study, the bacterial communities associated with the rhizosphere of Prosopis and Parthenium were characterized by clonal 16S rRNA gene sequence analysis. The culturable microbial counts in the rhizosphere of these two plants were higher than bulk soils, possibly influenced by the root exudates of these two plants. The phylogenetic analysis of V1_V2 domains of the 16S rRNA gene indicated a wider range of bacterial communities present in the rhizosphere of these two plants than in bulk soils and the predominant genera included Acidobacteria, Gammaproteobacteria, and Bacteriodetes in the rhizosphere of Prosopis, and Acidobacteria, Betaproteobacteria, and Nitrospirae in the Parthenium rhizosphere. The diversity of bacterial communities was more pronounced in the Parthenium rhizosphere than in the Prosopis rhizosphere. This culture-independent bacterial analysis offered extensive possibilities of unraveling novel microbes in the rhizospheres of Prosopis and Parthenium with genes for diverse functions, which could be exploited for nutrient transformation and stress tolerance in cultivated crops.

• Asia-Pacific Journal of Business
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• v.4 no.2
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• pp.31-40
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• 2013

This paper portrays the perception of buying behaviour, factors of product preferences and product switch-over among women consumers in Vellore district of Tamil Nadu. To conduct this study, 300 respondents from 20 village blocks of Vellore district were selected using stratified random sampling as the technique and descriptive sampling as the design. The study came out with interesting results and conclusions. It was found that more than half of the respondents were purchasing the product without a bill. It was also found that the respondents who repaired their product in the authorized service centre during warranty period did not continue the same after the warranty period and preferred nearby repair shops. It was further found that more than half of the respondents were checking expiry date and quality standards while purchasing a product. The study's scope was limited to women consumers at Vellore district. It could be used for further research in other aspects such as gender comparison in Vellore district and comparison with other districts of Tamil Nadu and other states of India. It was suggested that there is still a necessity to upgrade the standard of the people in many aspects like education, occupation and level of income, which shall help them to compete with the neighboring cities on living standards.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.11-11
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• 2002

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11a
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• pp.47-87
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• 2002

• International Journal of Knowledge Content Development & Technology
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• v.12 no.2
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• pp.77-98
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• 2022

These papers surveyed the respondents' Awareness and perception of Plagiarism among the Faculty members and research scholars of higher educational institutions in Dindigul District, Tamil Nadu. The 500 questionnaires were distributed among the participants, 456 questionnaires were filled and returned for usable by the respondents and remaining not replied. The responses rate is 91.2%. Further among 222 Male re- spondents, 11.18% of them are having Aware, 10.75% of them in Learning, 10.53% are with Fair and 76(16.23%) of them are Expert. Similarly out of 234 Female respondents, 16.45% of them has No Idea, 7.02% of them have Aware, 10.09% of them in Learning, 8.99% are with Fair and 8.77% of them are Expert.

• Journal of Korean Society for Geospatial Information Science
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• v.17 no.3
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• pp.3-12
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• 2009

Soil is one of the Earth's most important resources. There are many differences among the soils of plains.like and hilly terrains, and therefore, accurate and comprehensive information on soil is essential for optimum and sustainable soil utilization. However, information on the soil of the hilly terrains of the Eastern Ghats of Tamil Nadu, India, is limited or absent. In the present study, Kolli hill, one among the hills of the Eastern Ghats, was soil.inventoried and mapped using a ground survey and remote sensing. Soil samples were collected and their physico.chemical properties analyzed according to the United States Department of Agriculture (USDA) standards. The soils were classified up to the family level. As a result of this study, 30 soil series belonging to ten sub.groups of five great groups and three sub.orders and orders each, were identified (classified to the family level) and mapped. Entisols, Inseptisols and Alfisols were the three orders, among which Entisols was the major one, occupying 75% of the area. Among the five great groups, Ustorthents occupied majority of the area (73%). Lithic Ustorthents and Typic Ustorthents were the two major sub.groups, occupying 40% and 26% of the total area, respectively. The present soil resource mapping of the Eastern Ghats of Tamil Nadu is a pioneer study, which yielded valuable information on the soil in this region.

• The Journal of Asian Finance, Economics and Business
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• v.3 no.4
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• pp.81-90
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• 2016

The core purpose of the paper is to explore perceptual displays of rural India towards the role and impact of ICT in transforming the lives through education and telecommunications. After meticulous review of pertinent literature on ICT, and its policy framework, the researcher administers both quantitative and qualitative field data collected from the villages of Andhra Pradesh, Karnataka, Kerala, and Tamil Nadu with a sample of 250 from each state. More than 90 percent of respondents from the four southern states in India clearly thought that the role and impact of ICT is very momentous. Predominantly, cent percent respondents from Kerala evinced that ICT is the major reason for achieving the tag of "100% Literacy State". And also in the remaining three states viz., Andhra Pradesh, Karnataka, and Tamil Nadu, the literacy rate has crossed mind-boggling figures only because of ICT's reinforcement. Even in the arena of telecommunications, these four states average telecom density is more than the country's average. The paper concentrated only on how ICT function empirically to build human capacity in rural south-India through the sectors of only education and telecom. This study is first of its kind and precious to all stakeholders of the ICT sector like educational institutional, telecom firms, customers, employees, and governments, etc.

• Journal of Animal Science and Technology
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• v.57 no.10
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• pp.30.1-30.4
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• 2015

The present study was aimed to study the light and electron microscopic studies of thymic Hassall's corpuscles was done in various age groups of Nandanam Chicken ranging from day-old to forty weeks. Hassall's corpuscles are special, unique structures present in thymic medulla and also in the cortex of all the age groups of Nandanam chicken (from hatch to forty weeks) in the present study. Size of the Hassall's corpuscles in the medulla is larger than the ones present in the cortical region of thymus. The Hassall's corpuscles are made up of structureless eosinophilic mass surrounded by concentrically arranged reticuloepithelial cells. Under electron microscope, the Hassall's corpuscles were composed of reticuloepithelial cells interconnected by many desmosomes. The epithelial cells had abundance of cytoplasmic fibrils and desmosomes with few mitochondria and ribosomes. The nucleus was oval or round which was slightly indented. The centre of the Hassall's corpuscles was appeared either solid or cystic. The cystic corpuscles had cell debris within the cyst lumen.

• Korean Journal of Remote Sensing
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• v.24 no.6
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• pp.621-629
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• 2008

This study was carried out in part of Tamil Nadu, India. Also, Landsat ETM+ image and field sampling data were acquired. The field data were basal area, number of trees and number of species. Using the data set, this study performed a three steps processing, (1) Image classification (2) extracting the vegetation indices(NDVI, Tasseled cap brightness, greenness and wetness) (3) mapping the prediction of biodiversity distribution using basal area and NDVI image value. Basal area was significantly correlated with NDVI. The result of classification showed 69% overall accuracy.