DOI QR코드

DOI QR Code

Thymic Hassall's corpuscles in Nandanam chicken - light and electronmicroscopic perspective (Gallus domesticus)

  • Kannan, Thandavan Arthanari (Centre for Stem Cell Research and Regenerative Medicine Madras Veterinary College, Tamil Nadu veterinary and Animal Sciences University) ;
  • Ramesh, Geetha (Department of Veterinary Anatomy, Madras Veterinary College) ;
  • Ushakumary, S. (Department of Veterinary Anatomy, Madras Veterinary College) ;
  • Dhinakarraj, Gopalan (Translational Research Platform and Veterinary Biological Tamil nadu Veterinary and Animal Sciences University) ;
  • Vairamuthu, Subbiah (Central Clinical Laboratory Madras Veterinary College)
  • Received : 2015.05.26
  • Accepted : 2015.08.22
  • Published : 2015.10.31

Abstract

The present study was aimed to study the light and electron microscopic studies of thymic Hassall's corpuscles was done in various age groups of Nandanam Chicken ranging from day-old to forty weeks. Hassall's corpuscles are special, unique structures present in thymic medulla and also in the cortex of all the age groups of Nandanam chicken (from hatch to forty weeks) in the present study. Size of the Hassall's corpuscles in the medulla is larger than the ones present in the cortical region of thymus. The Hassall's corpuscles are made up of structureless eosinophilic mass surrounded by concentrically arranged reticuloepithelial cells. Under electron microscope, the Hassall's corpuscles were composed of reticuloepithelial cells interconnected by many desmosomes. The epithelial cells had abundance of cytoplasmic fibrils and desmosomes with few mitochondria and ribosomes. The nucleus was oval or round which was slightly indented. The centre of the Hassall's corpuscles was appeared either solid or cystic. The cystic corpuscles had cell debris within the cyst lumen.

Keywords

References

  1. Cortan RS, Kumar V, Robbin SL. Robbin's Pathogenic Basis of Disease. 4th ed. Philadelphia: W.B. Saunders; 1998. p. 163-4.
  2. Khan MZI, Masum A, Khan MZI, Aziz ARB, Nasrin M, Siddiqi MNH, et al. Histomorphology of the Lymphoid Tissues of Broiler Chickens in Kelantan, Malaysia. Sains Malaysiana. 2014;43(8):1175-9.
  3. Savino W, Dardenne M. Neuroendocrine control of thymus physiology. Endocr Rev. 2000;21:412-43.
  4. Ciriaco E, Pinera PP, Diaz-Esnal B, Laura R. Age related changes in the avian primary lymphoid organs (Thymus and Bursa of fabricius). Micros Res Tech. 2003;62:482-87. https://doi.org/10.1002/jemt.10416
  5. Hassan AU, Rasool Z. The Hassal of Thymus: Hassals Corpuscle Histological and Histopathological Perspective. Sch J App Med Sci. 2014;2(1B):147-8.
  6. Treesh SA, Buker AO, Khair NS. Histological, histochemical and immunohistochemical studies on thymus of chicken. Int J Histol Cytol. 2014;1(11):103-11.
  7. Ortiz-Hidalgo C. Early clinical pathologists 5: The man behind Hassall's corpuscles. J Clin Pathol. 1992;45:99-101. https://doi.org/10.1136/jcp.45.2.99
  8. Schuurman HJ, Kuper CF, Kendall MD. Thymic microenvironment at the light microscopic level. Micros Res Tech. 1997;38:216-26. https://doi.org/10.1002/(SICI)1097-0029(19970801)38:3<216::AID-JEMT3>3.0.CO;2-K
  9. Varga I, Pospisilova V, Jablonska V, Sisovsky V, Galfiova P, Polak S, et al. Thymic Hassall's bodies of children with congenital defects. Bratisl Lek Listy. 2010;111(10):552-7.
  10. Watanabe N, Wang YH, Lee HK, Ito T, Wang YH, Cao W, et al. Hassall's corpuscles instruct dendritic cells to induce $CD4^+$ $CD25^+$ regulatory T cells in human thymus. Nature. 2005;436:1181-5. https://doi.org/10.1038/nature03886
  11. Miclăuș V, Petrescu-Mag IV, Rus V, Cadar D, Oana L, Ober C. Frequency and polymorphism of Hassall's corpuscles in normal thymus of nutria (Myocastor coypus). AACL Bioflux. 2009;2(2):121-31.
  12. Rus V, Gal A, Ruxanda F, Bosca B, Nadas G, Ratiu C, et al. Morphostructural Characterization of Hassall's Corpuscles in Lamb. Bulletin UASVM Vet Med. 2014;71(2):1-6.
  13. Gilmore RSC, Bridges JB. Histological and ultrastructural studies on the myoid cells of the thymus of the domestic fowl, Gallus domesticus. J Anat. 1974;118:409-16.
  14. Bancroft JD, Layton C, Suvarna SK. Bancroft's Theory and Practice of Histological Techniques. 7th ed. Elsevier: Churchill Livingstone; 2013.
  15. Kannan TA, Ramesh G, Ushakumari S, Dhinakarraj G, Vairamuthu S. Light microscopic studies on Spleen of Chicken (Gallus domesticus). Haryana Vet. 2012;51(12):114-5.
  16. Kannan TA, Geetha R, Ushakumari S, Dhinakarraj G, Vairamuthu S. Electron Microscopic Studies of Spleen in Chicken (Gallus domesticus) (Gallus domesticus). Int J of Adv Vet Sci Tech. 2015;4(1):160-5. https://doi.org/10.23953/cloud.ijavst.180
  17. Trautmann A, Fiebiger J. Fundamentals of the histology of domestic animals. New York: Comstock Pub. Assoc; 1957. p. 142-43.
  18. Leena C, Prasad RV, Kakade K, Jamuna KV. Histology and age related involutary changes of the thymus of Giriraja birds (Gallus domesticus). J Vet Anim Sci. 2008;39:40-3.
  19. Haseeb A, Shah MG, Gandahi JA, Lochi GM, Khan MS, Faisal M, et al. Histo-morphological Study on Thymus of Aseel chicken. J Agric Food Tech. 2014;4(2):1-5.
  20. Kendall MD, Singh J. The presence of erythroid cells in the thymus gland of man. J Anat. 1980;130:183-89.
  21. Tarek K, Mohamed M, Omar B, Hassina B. Morpho-Histological study of the thymus of broiler chickens during Post-Hashing Age. Int J Poult Sci. 2012;11(1):78-80. https://doi.org/10.3923/ijps.2012.78.80
  22. Bridges JB, Gilmore PR, Morris GP. The ultrastructure of the thymus of the cockrel. J Anat. 1970;107:388-89.
  23. Hashimoto Y, Sugimura M. Histological and quantitative studies on the postnatal growth of the thymus and the bursa of Fabricius of white Pekin ducks. Jap J Vet Res. 1976;24:65-76.
  24. Robert MV, Bensch KG, Levin GD. The normal human thymic vasculature: An ultrastructural study. Anat Rec. 1978;183:485-98.
  25. Frazier JA. Ultrastructure of the chick thymus. Zellgasch. 1973;136:191-205.
  26. Blau JN. Hassall's corpuscles- a sight of thymocyte death. Br J Exp Path. 1973;54:634-7.
  27. Olsson L, Classon MH. Studies on the regulation of lymphocyte production in the murine thymus and some effects of crude thymus extract. Cell Tis Res. 1975;8:491.
  28. Senelar R, Escola MJ, Serron B, Seree A. Relationship between Hassall's corpuscles and thymocytes in guinea pig foetus. Biomedicine. 1976;24:112-22.
  29. Chan AS. Ultrastructure of epithelial thymic cysts of the chicks. Poult Sci. 1986;65:177-82. https://doi.org/10.3382/ps.0650177
  30. Goldstein AL, Asanuma Y, White A. The thymus as an endocrine gland: properties of thymosin, a new thymus hormone. Recent Progr Hormone Res. 1970;26:505-38.
  31. Hoshino T. Electron microscopic studies of the epithelial reticular cells of the mouse thymus. Z Zellforsch. 1963;59:513-29. https://doi.org/10.1007/BF00368725
  32. van Haelst U. Light and electronmicroscopic study of the normal and pathological thymus of the rat. I the normal thymus. Z Zellforsch Mikrosk Anat. 1967;77:534-53. https://doi.org/10.1007/BF00319347
  33. Clark Jr SL. The thymus in mice of strain 129/J studied with the electron microscope. Americian J Anat. 1963;112:1-33. https://doi.org/10.1002/aja.1001120102
  34. Henry K. Mucin secretion and striated muscles in the human thymus. Lancet. 1966;I:183-85.
  35. Pfoch M. Vergleichende elektronenmikroskopische Untersuchung an entodermalen Thymus-Retikulumzellen neugeborener und alter Wistar-Ratten. Z Zellforsch. 1971;114:271-80. https://doi.org/10.1007/BF00334006
  36. Cesarini JP, Benkoel L, Bonneau H. Ultrastructure compare du thymus chez le hamsterjeune et adulte. C R Soe Biol (Paris). 1968;162:1975-79.
  37. Chapman WL, Allen JR. The fine structure of the thymus of the fetal and neonatal monkey (Macaca mulatta). Z Zellforsch. 1971;114:220-33. https://doi.org/10.1007/BF00334002
  38. Izard J. Ultrastructure of the thymic reticulum in G. Pig Anat Rec. 1966;155:117-32. https://doi.org/10.1002/ar.1091550114

Cited by

  1. Morphological view on the evolution of the immunity and lymphoid organs of vertebrates, focused on thymus vol.71, pp.10, 2016, https://doi.org/10.1515/biolog-2016-0137
  2. Post-natal macro- and microscopic changes of the thymus of Sonali chicken in Bangladesh vol.7, pp.2, 2015, https://doi.org/10.5455/javar.2020.g425