• IMMUNE NETWORK
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• 제5권3호
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• pp.179-185
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• 2005

Background: Psoriasis is a multifactorial autoimmune skin disease with a pathogenesis that has remained obscure. Recently, T cells bearing natural killer receptors (NKRs) were precisely and strongly targeted as new putative pathogenic immunocytes in psoriasis. Among NKRs, killer cell immunoglobulin-like receptor (KIR) is the major molecule recognizing HLA class I allotypes and might be closely related to psoriasis. Methods: To investigate the association of KIR genotype and patients with psoriasis in Korean, we defined the 14 KIR genotypes in 96 patients with psoriasis and 86 healthy controls using PCR-SSP methods. Results: The frequencies of KIR2DS4 and KIR3DL1 were significantly decreased in psoriasis compared with controls (RR=0.21, p<0.02). When patients were divided into two subgroups at the age of onset, type I (<30 years) and type II ($({\geq}30$ years) respectively, these phenomena were similarly observed independent of groups divided (type I: RR=0.26, p<0.005; type II: RR=0.14, p<0.0006). When the patients were divided into subgroups according to the age of onset and family history, the frequencies of KIR2DS4, KIR3DL1, and KIR2DS3 were significantly decreased in type I compared with type II psoriasis (3DL1, 2DS4: p<0.004; 2DS3: p<0.04) and were significantly decreased in psoriasis without family history compared to with family history (3DL1, 2DS4: p<0.007; 2DS3: p<0.05). The frequency of haplotype combination BB was significantly increased in psoriasis compared with controls (RR=2.74, p<0.009). Conclusion: These results suggest that KIR genotype is a factor for the occurrence and development of psoriasis and in future how combinations of HLA and KIR genes influence psoriasis needs to be defined.

• 대한한방내과학회지
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• 제13권1호
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• pp.61-69
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• 1992

In Nan-Gyung, showed that could know the lung condition taking pulse with the weight of three beans, the heart condition taking pulse with the weight of six beans, the spleen condition taking pulse with the weight of nine beans, the liver condition taking pulse with the weight of twelve beans, the kidney condition pressing to bone(骨). This theory is first suggested in Nan-Gyung(難經). In those case, the weight of three, six, nine, twelve beans and pressing to bone don't mean not the real weight but the relative weight(輕重) of taking pulse(按脈). In other words, those represent Boo Jung Chin(浮中沈), which are the conception of the upper, the meddle, the lower part(上中下). So, we could take pulse of the heart and the lung condition in Boo(浮), the spleen condition in Jung(中), and the liver and the kidney condition in Chim(沈). The heart and the lung pulse showed in the Boo(float level) must be seen with Boo-Mack(부맥 : float pulse), the liver and the kidney pulse showed in Chim (sinklevel) must be seen also with Chin-Mack(沈脈 : sink pulse). The result of the method of taking pulse of viscera with relative weight focused on the as pect of mornal pulse(平脈) and disease pulse(病脈) of five viscera in Mack-Gyung publeshed later than Nan-Gyung and special works which made a comprehensive survey the result is as follow. 1. In normal pulse of five viscera, the heart and the lunk pulse were shown with Boo-Mack(浮脈:float pulse) as the central figure, the liver's and the kidney's pulse were shown centering around Chim-Mack(沈脈: sink pulse) and the spleen's pulse was shown with Wan-Mack(緩脈) which is vital force of stomach(胃氣) and seen in only middle part. 2. In disease pulse of five viscera, frequently, the heart and the lung pulse was shown as Chim-Mack(sink pulse), the liver and the kidney pulse was seen as Boo-Mack (float pulse). 3. In the case of normal pulse. the method of taking pulse with relative weight in Nan-Gyung agree with the normal pulse of five viscera in Mack-Gyung. But in the case of disease pulse, they didn't correspond with the other. 4. So the method of taking pulse with relative weight in Nan-Gyung is not the exam pulse which ca be used in the clinical diagnosis but one of the feeling pulse way to bring in the conception of location of the visceras. 5. From now on, the method of taking pulse rdlated to relative weight need to be looked into minutely compared with later physician's theory than Mack-Gyung.

• Natural Product Sciences
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• 제18권1호
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• pp.60-66
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• 2012

Human skin is the first line of defense for the protection of the internal organs of the body from different stimuli. Ultraviolet B (UVB) irradiation induces skin damage and inflammation through the secretion of various cytokines, which are immune regulators produced by cells. To prevent the initiation of skin inflammation, keratinocytes that have been irreversibly damaged by radiation must be removed through the apoptotic mechanism. Ixeris dentata (family: Asteraceae) is a perennial medicinal herb indigenous to Korea. It has been used in Korea, China, and Japan to treat in digestion, pneumonia, diabetes, hepatitis, and tumors. To gain insight into the anti-inflammatory effects of I. dentata, we examined its influence on UVB-induced pro-inflammatory cytokine production in human keratinocytes (HaCaT cells), by observing cells that were stimulated with UVB in the presence or absence of I. dentata. In the present study, pro-inflammatory cytokine production was determined by performing enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and western blot analysis to measure the activation of mitogen-activated protein kinase (MAPKs). I. dentata inhibited UVBinduced production of the pro-inflammatory cytokine interleukin (IL)-6 in a dose-dependent manner. Further, I. dentata inhibited the UVB-induced expression of cyclooxygenase (COX)-2. Furthermore, I. dentata inhibited the phosphorylation of c-Jun NH2-terminal kinase and p38 MAPKs, suggesting that it inhibits the secretion of the pro-inflammatory cytokines IL-6 and IL-8, and COX-2 expression, by blocking MAPK phosphorylation. These results suggest that I. dentate can potentially protect against UVB-induced skin inflammation.

• 대한조선학회논문집
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• 제47권4호
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• pp.628-645
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• 2010

To perform the engagement level simulation between the underwater vehicle model and the surface model those are constituted with various systems/ sub-systems, we implemented four different federates as a federation according to the IEEE 1516 HLA (High Level Architecture) protocol that is the international standard in the distributed simulation. Those are CFCS (Command and Fire Control System) federate, motion federate, external entities (torpedos, countermeasure and surfaceship) federate, and visualization federate that interacts with OSG (Open Scene Graph)-based visualization rendering module. In this paper, we present the detailed method about the model constitution for discrete event simulation in the distributed environment. For the sake of this purpose, we introduce the DEVS (Discrete Event System Specification)-HLA-based modeling method of the CFCS federate that reflects not only the interations between models, but also commands from user and tactics manager that is separated from the model. The CFCS federate makes decisions in various missions such as the normal diving, the barrier misision, the target motion analysis, the torpedo launch, and the torpedo evasion. In the perspective of DEVS modeling, the CFCS federate is the coupled model that has the tactical data process model, command model and fire control model as an atomic model. The message passing and time synchronization with other three federates are settled by the $m\ddot{a}k$ RTI (Runtime Infrastructure) that supports IEEE 1516. In this paper, we provides the detailed modeling method of the complicated model that has hierarchical relationship such as the CFCS system in the submarine and that satisfies both of DEVS modeling method for the discrete event simulation and HLA modeling method for the distributed simulation.

• Radiation Oncology Journal
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• 제34권3호
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• pp.193-201
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• 2016

Purpose: To assess the clinical outcomes of radiotherapy (RT) using two-dimensional (2D) and three-dimensional conformal RT (3D-CRT) for patients with gastric mucosa-associated lymphoid tissue (MALT) lymphoma to evaluate the effectiveness of involved field RT with moderate-dose and to evaluate the benefit of 3D-CRT comparing with 2D-RT. Materials and Methods: Between July 2003 and March 2015, 33 patients with stage IE and IIE gastric MALT lymphoma received RT were analyzed. Of 33 patients, 17 patients (51.5%) were Helicobacter pylori (HP) negative and 16 patients (48.5%) were HP positive but refractory to HP eradication (HPE). The 2D-RT (n = 14) and 3D-CRT (n = 19) were performed and total dose was 30.6 Gy/17 fractions. Of 11 patients who RT planning data were available, dose-volumetric parameters between 2D-RT and 3D-CRT plans was compared. Results: All patients reached complete remission (CR) eventually and median time to CR was 3 months (range, 1 to 15 months). No local relapse occurred and one patient died with second primary malignancy. Tumor response, survival, and toxicity were not significantly different between 2D-RT and 3D-CRT (p > 0.05, each). In analysis for dose-volumetric parameters, $D_{max}$ and CI for PTV were significantly lower in 3D-CRT plans than 2D-RT plans (p < 0.05, each) and $D_{mean}$ and V15 for right kidney and $D_{mean}$ for left kidney were significantly lower in 3D-CRT than 2D-RT (p < 0.05, each). Conclusion: Our data suggested that involved field RT with moderate-dose for gastric MALT lymphoma could be promising and 3D-CRT could be considered to improve the target coverage and reduce radiation dose to the both kidneys.

• BMB Reports
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• 제44권12호
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• pp.799-804
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• 2011

Gangliosides play an important role in neuronal differentiation processes. The regulation of ganglioside levels is related to the induction of neuronal cell differentiation. In this study, the ST8Sia5 gene was transfected into mESCs and then differentiated into neuronal cells. Interestingly, ST8Sia5 gene transfected mESCs expressed GQ1b by HPTLC and immunofluorescence analysis. To investigate the effects of GQ1b over-expression in neurogenesis, neuronal cells were differentiated from GQ1b expressing mESCs in the presence of retinoic acid. In GQ1b expressing mESCs, increased EBs formation was observed. After 4 days, EBs were co-localized with GQ1b and nestin, and GFAP. Moreover, GQ1b co-localized with MAP-2 expressing cells in GQ1b expressing mESCs in 7-day-old EBs. Furthermore, GQ1b expressing mESCs increased the ERK1/2 MAP kinase pathway. These results suggest that the ST8Sia5 gene increases ganglioside GQ1b and improves neuronal differentiation via the ERK1/2 MAP kinase pathway.

• 대한한의학방제학회지
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• 제13권2호
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• pp.101-110
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• 2005

The present study was conducted to compare antioxidant activity of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba by DPPH radical scavenging activity. The extract was studied using diphenyl-picryl-hydrazyl (DPPH) for DPPH method. DPPH radical scavenging activity was measured after. .10, 20 and 30 minutes. The extract was tested by 1, 5, 10, 50, 100, 500 and $1000{\mu}g/ml$ concentrations. The results showed that the extract scavenged DPPH radical with time-dependent manner. Also, the extract showed dose-dependent DPPH radical scavenging activity. The extract of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba scavenged DPPH radical with the IC50 being 2.7, 3.2, 2.9 and 1.1 mg/ml, respectively. In conclusion, the extract of Epimedii Herba, Cuscutae Semen, Cnidii Fructus and Psoraleae Fructus have antioxidant activity for the treatment of male sterility.

• Natural Product Sciences
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• 제26권1호
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• pp.83-89
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• 2020

Osteoporosis is a worldwide disease leading to significant economic and societal burdens globally. Osteoporosis is caused by unbalanced bone remodeling between the rate of osteoclast bone resorption and osteoblast bone formation. Acer tegmentosum Maxim (AT) is a traditional herbal medicine containing multiple biological activities such as anti-oxidant and anti-inflammatory purposes. However, its role in osteoporosis has not been fully studied. Therefore, we investigated whether AT has a potent inhibitory effect on osteoporosis and its mechanism through a systemic evaluation in ovariectomized (OVX) mice. OVX mice were orally administrated with the AT at doses of 50, 100, and 200 mg/kg for 10 weeks. Histological images and histomorphometry analyses were performed by H&E and Toluidine blue satin, and the expression levels of receptor activator for nuclear factor-kB ligand (RANKL), nuclear factor of activated T cells cytoplasm 1 (NFATc1), c-Fos, and matrix metalloproteinase 9 (MMP9) related to the osteoclast differentiation were investigated using immunohistochemical analysis. Administration of AT prevented bone loss and the alternations of osteoporotic bone parameters at the distinct regions of the distal femur and spongiosa region in OVX mice. Further, administration of AT increased periosteal bone formation in a dose-dependent manner. Meanwhile, AT inhibited not only the expression of NFATc1 and c-Fos, which are two major regulators of osteoclastogenesis but also reduced bone resorbed encoding expression of MMP9 and RANKL. Our results indicated that administration of AT prevented bone loss and the alternations of osteoporotic bone parameters at the distinct regions of the distal femur and spongiosa region in OVX mice. Also AT has the bone protective effect through the suppression of osteoclast and promotion of osteoblast, suggesting that it could be a preventive and therapeutic candidate for anti-osteoporosis.

• 한국식품과학회지
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• 제44권2호
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• pp.179-184
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• 2012

홍삼 특유의 prosapogenin 성분을 극대화 시키기 위해 홍삼의 증숙, 건조 및 추출 공정을 최적화하여 식초에 첨가할 홍삼 농축액을 제조하였다. 농축액을 0-1%의 범위로 첨가한 홍삼 발효 쌀 식초의 경우 정치 배양 20일차에 초산발효가 종료 되었으며 홍삼 농축액의 함유량이 높을수록 pH 감소속도가 늦고 감소량 또한 control에 비해 적음을 확인 할 수 있었다. 산도의 경우 농축액 농도 별 초산 생성 속도에는 차이는 있으나 농축액 함유량이 산도 변화 량에 큰 영향을 미치지 않았다. 1%의 홍삼 농축액을 후 첨가한 식초의 경우 71.75 mg/g의 조사포닌 함량을 가진 반면에 0-1% 범위의 농축액을 주모에 첨가하여 초산 발효를 동시에 진행한 경우 발효 진행 중 상당한 조사포닌 손실이 있음을 확인하였다. 이 두 식초를 가지고 관능검사를 진행한 결과 향기, 맛 및 전체적인 평가에서 농축액과 초산발효를 동시에 진행시킨 홍삼발효식초가 더 좋은 선호도를 나타내었다.

• 분석과학
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• 제23권2호
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• pp.147-154
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• 2010

본 연구에서는 최근 신종플루가 유행하여 수용성이 큰 항바이러스제인 타미플루(Oseltamivir ethylester phosphate, OEP)가 다량 사용됨에 따라 환경 중 잔류농도를 파악하고, 수생태 영향을 평가하고자 하천수를 채취하여 분석하였다. 타미플루의 주성분인 오설태미비르 에틸에스터(Oseltamivir ethylester, OE) 및 활성대사체 오설태미비르산(Oseltamivir carboxylate, OA)이 모든 지점에서 각각 $0.008\sim0.087\;{\mu}g/L$, $0.029\sim0.287\;{\mu}g/L$의 범위로 검출되었으며, OE보다 활성대사체(OA)가 상대적으로 높게 검출되었다. 이는 신종플루 대유행 이전인 일본 수질조사 결과에 비해 OA는 2배 높게 나타났으나, 시료채취 시기가 달라 직접적인 비교는 어려운 것으로 판단되었다.