• Title/Summary/Keyword: TLC pattern

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Plastein formation from sunflower seed protein (해바라기씨 단백질에서 plastein의 합성)

  • Rho, Jae-Mun;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.34 no.1
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    • pp.1-7
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    • 1991
  • Optimum conditions for hydrolysis of sunflower seed by pepsin and for plastein formation by pepsin were determined. The optimum conditions for hydrolysis of sunflower seed were pH 1.5, $45^{\circ}C$, enzyme concentration 2%, substrate concentration 2%, and hydrolysis time 24hr. The optimum conditions for sunflower seed-plastein formation were 50% substrate, pH 4.5, $50^{\circ}C$, 0.25% pepsin and 18hrs reaction time. To verify plastein fromation from concentrated prptic hydrolysate of sunflower seed, thin layer chromatography was performed. The TLC pattern of concentrated peptic hydrolysate of sunflower seed was different from that of its plastein. The TLC pattern of concentrated peptic bydrolysate of sunflower seed and at of its plastein indicated that plastein was different material from the hydrolysate.

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Studies on the Analysis of Constituents of Deer Horn(I) -Assay of Trace Elements and TLC Pattern Analysis of Gangliosides- (산지별 녹용(鹿茸)류의 성분분석 연구(I) -미량금속의 정량 및 Gangliosides의 TLC Pattern 분석-)

  • Hong, Nam-Doo;Won, Do-Hee;Kim, Nam-Jae;Chang, Seung-Youb;Youn, Whang-Geum;Kim, Hae-Soo
    • Korean Journal of Pharmacognosy
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    • v.22 no.3
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    • pp.171-182
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    • 1991
  • Several experiments were carried for the purpose of establishing the basis for the quality evaluation of deer horn. Deer horn originated from China, New Zealand, Soviet and Alaska were used as objectives and some constituents involved in deer horn were assayed and compared with one another. Five kinds of trace elements including Ca and Fe were determined by atomic absorbance spectroscopy. The more the region came down from the top of the deer horn, the more the ratio of Ca to Fe content (Ca/Fe) was increased. The same increasing tendancy was observed in the case of ash content. Gangliosides were isolated from deer horn by alumina column chromatography and identified by TLC using gangliosides Type II obtained from bovine brain as the standard material. The TLC chromatograms were scanned by dual wavelength TLC scanner, then those TLC profiles were compared. Deer horn from China and New Zealand were analogous but those from Soviet and Alaska were each distinguished in their TLC profiles.

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NAND-Type TLC Flash Memory Test Algorithm Using Cube Pattern (큐브 패턴을 이용한 NAND-Type TLC 플래시 메모리 테스트 알고리즘)

  • Park, Byeong-Chan;Chang, Hoon
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2018.07a
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    • pp.357-359
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    • 2018
  • 최근 메모리 반도체 시장은 SD(Secure Digital) 메모리 카드, SSD(Solid State Drive)등의 보급률 증가로 메모리 반도체의 시장이 대규모로 증가하고 있다. 메모리 반도체는 개인용 컴퓨터 뿐만 아니라 스마프폰, 테플릿 PC, 교육용 임베디드 보드 등 다양한 산업에서 이용 되고 있다. 또한 메모리 반도체 생산 업체가 대규모로 메모리 반도체 산업에 투자하면서 메모리 반도체 시장은 대규모로 성장되었다. 플래시 메모리는 크게 NAND-Type과 NOR-Type으로 나뉘며 플로팅 게이트 셀의 전압의 따라 SLC(Single Level Cell)과 MLC(Multi Level Cell) 그리고 TLC(Triple Level Cell)로 구분 된다. SLC 및 MLC NAND-Type 플래시 메모리는 많은 연구가 진행되고 이용되고 있지만, TLC NAND-Tpye 플래시 메모리는 많은 연구가 진행되고 있지 않다. 본 논문에서는 기존에 제안된 SLC 및 MLC NAND-Type 플래시 메모리에서 제안된 큐브 패턴을 TLC NAND-Type 플래시 메모리에서 적용 가능한 큐브 패턴 및 알고리즘을 제안한다.

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How Many Patients with Mixed Spirometric Pattern Really Have Restrictive Disorders? (폐활량 검사 상 혼합성 환기 장애를 보일 때 실제 얼마나 제한성 장애를 동반하는가?)

  • Oh, Yeon-Mok
    • Tuberculosis and Respiratory Diseases
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    • v.47 no.6
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    • pp.836-842
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    • 1999
  • Background: Mixed obstructive and restrictive pattern of spirometry can not be concluded in the presence of true restrictive disorders because pure obstructive disorders can also show reduced vital capacity. However, it is not known how many patients with mixed spirometric pattern really have restrictive disorders in Korea whose pattern of pulmonary diseases is somewhat different from foreign countries. To answer this question, I performed this study and tried to answer it according to diseases in addition. Method: Test results from 413 patients who undergone both spirometry and lung volume measurements on the same visit from August 1, 1998 to July 31, 1999 were included. Spirometry data were classified as mixed obstructive-restrictive pattern when spirometry showed '$FEV_1/FVC$<70% (<65% if age$\geq$60)' and FVC<80% of predicted value'. TLC by the method of nitrogen washout was considered as gold standard to diagnose restrictive disorders in which TLC is less than 80% of predicted value. Results: Out of 404 patients who could be evaluated, 58 had mixed pattern of spirometry. 58 patients were suffered from airway diseases(39 patients) such as COPD(22 patients, 38%), asthma(11, 19%), bronchiectasis (6,10%), and sequelae of pulmonary tuberculosis(15, 26%) or other diseases(4,7%). Only 18 out of 58(31%) were confirmed to have true restrictive disorders by TLC. The proportion of true restrictive disorders was different according to diseases, 20.5%(8/39patients) in patients with airway diseases and 53.3%(8/15) with sequelae of pulmonary tuberculosis(p<0.05). Conclusion: Many patients whose spirometry showed mixed pattern didn't have restrictive disorders but had pure obstructive disorders. This was true for more patients with airway diseases. Therefore it would be prudent that lung volume be tested to diagnose restrictive disorders in patients with mixed spirometric pattern.

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Chemical Studies on the Alkaloidal Fraction of Panax ginseng C.A. Meyer (I). TLC Analysis of Various Ginsengs and Identification of an Alkaloid Component (고려인삼 알카로이드 분획의 성분연구 (I) 인삼류별 TLC 비교 및 알카로이드 동정)

  • 박종대;위재준
    • Journal of Ginseng Research
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    • v.11 no.1
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    • pp.17-23
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    • 1987
  • A crude alkaloidal fraction from white ginseng (Panax.gilfsertg C.A. Meyer) showed over thirteen Dragendorff positive spots by TLC using eluent of $CHCl_3$/MeOH(10 : 1). TLC pattern of white, red and Sanchii ginseng (P. notoginseng) was mostly not different from each other, but, in American ginseng (P. quinqgtefolium),two spots having Rf 0.71 and 0.68 were not detected. An alkaloid component was isolated from white ginseng and identified as $N_9$-formyl-1-methyl-$\beta$-carboline (mp $174^{\circ}C$, $C_13H_10N_2O$).

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Physico-Chemical Identification of Botanical Drugs (생약(한약재(漢藥材))의 규격제정 연구 -TLC를 이용한 정성적 검출법-)

  • Chi, Hyung-Joon;Won, Do-Hi
    • Korean Journal of Pharmacognosy
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    • v.3 no.4
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    • pp.199-204
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    • 1972
  • A new physico-chemical method for identifying botanical drugs was investigated. Fifty drugs were chosen from the presently exported botanical drugs and were classified into 12 groups chemotaxonomically. Various extract(either, 50% ethanol and water) of each group were developed with same solvent system by using chromatographic method(TLC) and observed a characteristic pattern of each drugs. This method, therefore, can be applicable to identify each botanical drug out of the combined phyto-preparations.

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Isolation and Identification of Anticancer Compounds from Eucommia ulmoides Leaves (두충잎의 항암성분 분리 및 동정)

  • 김종배;박정륭;전정례;차명화
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.4
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    • pp.732-738
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    • 2001
  • This study was attempted to isolate and identify the anticancer compounds from Eucommia ulmoides leaves using a human colon cancer cell line HCT-116. The petroleum ether extracts with anticancer activity was chromatographed on silica gel TLC and finally anticancer compounds was purified by HPLC. Their chemical structures were roughly elucidate by UV-VIS absorption spectral data HPLC elution pattern and FAM/MS spectroscopy. From this study these compounds were suspected to be pheophytin a formed by the removal of $Mg^{2+}$ from chlorophyll a and pyropheophytina formed by the removal of acetate group from pheophytin a respectively. To confirm the anticancer effects against HCT-116 cancer cell petroleum ether extract fractions of column chromatography and fractions separated on TLC were tested. All samples tested including the extract of petroleum ether fractions of column chromatograph and three bands (0.13,0.19,0.25) of TLC appeared to inhibit the growth of HCT-116 cancer cell however especially 0.19 and 0.25 fractions separated on TLC plate revealed the strongest effect. These results suggest that chlorophyll derivatives in Eucommia ulmoides may be potential anticancer agents against a human colon cancer cell HCT-116.

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Comparative Study of the Rhei Rhizoma by Pattern Analysis (패턴분석법에 의한 대황의 비교 연구)

  • Kang, Jong-Seong;Park, Ki-Ju;Wu, En-Qi;Lee, Eun-Sil;Hwang, Gwi-Seo;Lee, Hyun-Sun;Kim, Young-Ho
    • Korean Journal of Pharmacognosy
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    • v.39 no.3
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    • pp.179-185
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    • 2008
  • Three species, such as Rheum palmatum L., R. tanguticum Maxim. and R. officinale Baillon are recognized as the source plants of Rhei Rhizoma in Korean Pharmacopeia. However, other herbal sources such as R. undulatum L. and Rumex crispus L. have been often misused as Rhei Rhizoma. A pattern analysis method to discriminate Rhei Rhizoma in Korean Pharmacopeia from other herbal plants using HPLC and TLC chromatograms was developed. The multivariate peak data of the chromatograms of methanol extracts of Rhei Rhizoma were used for hierarchical clustering analysis, principal components analysis and similarity calculation. Besides of the statistic analysis, TLC patterns of samples could be used as criteria of the discrimination. The developed pattern analysis method was specific and could be readily utilized for comprehensive evaluation of Rhei Rhizoma.

Studies on the Analysis of Constituents of Deer Horn(I) - Assay of Trace Elements and TLC Pattern Analysis of Gangliosides (산지별 녹용류의 성분분석 연구(I) - 미량금속의 정량 및 Gangliosides의 TLC Pattern 분석)

  • Hong Nam Doo;Won Do Hee;Kim Nam Jae;Chang Seung Youb;Youn Whang-Geum;Kim Hae-Soo
    • Korean Deer Journal
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    • no.14
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    • pp.12-21
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    • 1991
  • Several experiments were carried for the purpose of establishing the basis for the quality evaluation of deer horn. Deer horn originated from China, New Zealand, Soviet and Alaska were used as objectives and some constituents involved in deer horn were as

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Antibacterial Activity of Some Chrysanthemum spp. (Chrysanthemum속(屬) 식물의 항균성)

  • Nam, Sang-Hae;Choi, Sang-Uk;Yang, Min-Suk;Jang, Dae-Sik
    • Applied Biological Chemistry
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    • v.39 no.4
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    • pp.315-319
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    • 1996
  • The yields of methanol extracts of several Chrysanthemum spp.(5 species, 9 parts) and their solvent fractions were investigated. The yields of methanol extracts ranged from 16.9%(for flower of C. indium, Cultivated) to 31.5%(for whole plant of C. indium). In the tests of the antibacterial activity. the methanol extracts from flower of C. zawadskii and C. boreale, whole plant of C. zawadskii, and flower of C. coronarium showed excellent antibacterial activity. Generally, the chloroform fractions exhibited stronger antibacterial activity against all the bacteria tested when compared with other solvent fractions. In case of C. boreale, the chloroform fraction of the whole plant was shown stronger antibacterial activity against all four bacterial strains tested when compared with that of the flower. The chloroform fractions from flower and whole plant of C. boreale and C .zawadskii, and flower of C. coronarium were shown a similar TLC pattern.

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