Studies on the Analysis of Constituents of Deer Horn(I) -Assay of Trace Elements and TLC Pattern Analysis of Gangliosides-

산지별 녹용(鹿茸)류의 성분분석 연구(I) -미량금속의 정량 및 Gangliosides의 TLC Pattern 분석-

  • Hong, Nam-Doo (Department of Pharmaceutics, kyung-Hee University Medical Center) ;
  • Won, Do-Hee (Department of Drugs, National Institute of Health) ;
  • Kim, Nam-Jae (Department of Pharmaceutics, kyung-Hee University Medical Center) ;
  • Chang, Seung-Youb (Department of Drugs, National Institute of Health) ;
  • Youn, Whang-Geum (Department of Pharmaceutics, kyung-Hee University Medical Center) ;
  • Kim, Hae-Soo (Department of Drugs, National Institute of Health)
  • Published : 1991.09.30

Abstract

Several experiments were carried for the purpose of establishing the basis for the quality evaluation of deer horn. Deer horn originated from China, New Zealand, Soviet and Alaska were used as objectives and some constituents involved in deer horn were assayed and compared with one another. Five kinds of trace elements including Ca and Fe were determined by atomic absorbance spectroscopy. The more the region came down from the top of the deer horn, the more the ratio of Ca to Fe content (Ca/Fe) was increased. The same increasing tendancy was observed in the case of ash content. Gangliosides were isolated from deer horn by alumina column chromatography and identified by TLC using gangliosides Type II obtained from bovine brain as the standard material. The TLC chromatograms were scanned by dual wavelength TLC scanner, then those TLC profiles were compared. Deer horn from China and New Zealand were analogous but those from Soviet and Alaska were each distinguished in their TLC profiles.

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