• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.14 no.6
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• pp.50-59
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• 2015

This study aimed at suggesting Willingness To Pay (WTP) for introduction of the Traffic Accident emergency Call (TAC) system by using Contingent Valuation Method (CVM) which is a general valuation method. As the method, this study suggested a WTP estimation method of the TAC system with the double-bound dichotomous choice model. In previous studies, the data are processed differently according to the type of questions and analysis models used for the calculation of willingness to pay. Therefore, we re-organized the model by the cases using the truncated data sets, and showed the difference in WTPs. The dataset was developed by more than 500 questionnaire obtained from online and offline survey with the consideration of composition ratio by age group referring housing census in 2010 to mitigate regional bias of samples. At last, this study applied various statistical methods, survival analysis, multiple regression, and Tobit model for better interpretation of the questionnaires.

• Proceedings of the Korean Society of Computer Information Conference
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• 2010.07a
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• pp.393-396
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• 2010

생산자로부터 고객에 이르는 상품 공급 활동을 관리하는 경영기법인 공급사슬관리(SCM)는 기존의 폐쇄적이고 정적인 환경에서 개방적이고 동적인 환경으로 변화하고 있다. 이러한 변화에 대응하기 위한 실험이나 연구는 부족한 상황이다. TAC SCM은 동적인 SCM 환경에서 다른 경쟁자들과의 경쟁을 통하여 다양한 SCM 전략을 시험할 수 있다. 본 연구에서는 실시간 SCM 환경에서 능동적 할인 전략을 적용한 에이전트를 설계 및 구현하였다. 능동적 할인은 입찰 성공률에 따라 할인율을 변경하여 적용하여 항상 일정한 생산량을 보유할 수 있도록 한다. 본 연구의 실험 결과에서 제안한 에이전트가 서버에서 제시한 Dummy 에이전트보다도 높은 수익과 안정된 생산을 할 수 있음을 보였다.

• Korean Journal of Agricultural Science
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• v.41 no.3
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• pp.245-249
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• 2014

Among dozens of DNA polymerases cloned from thermophilic bacteria, Taq DNA polymerase from Thermus aquaticus has been most frequently used in polymerase chain reaction (PCR) that is being applied to gene cloning, DNA sequencing, gene expression analysis, and detection of infectious and genetic diseases. Since native Taq DNA polymerase is expressed at low level in T. aquaticus, recombinant Escherichia coli system was used to produce Taq DNA polymerase in a large amount. Taq DNA polymerase was expressed as a soluble form under the control of tac promoter in E. coli, and purified by heat treatment and ion exchange chromatographies. The purified Taq DNA polymerase was nearly homogeneous and exhibited a similar DNA amplification activity with a commercial Taq DNA polymerase.

• Journal of Food Hygiene and Safety
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• v.16 no.3
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• pp.194-199
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• 2001

This study was designed to compare the effectiveness and applicability of the HApS$^{TM}$ (Hazard Analysis process System; HUKO, Seoul, Korea) based on Petrifilm$^{TM}$ (3M, St. Paul, MN, USA) with the AOAC (the Association of Official Analytical Chemists) standard total aerobic count (TAC) method and coliform count (CC) method for meat products. The comparisons were carried out using 230 meat samples collected from various retailers: 80 pork samples, 80 chicken samples, and 70 beef samples. In the comparison of the correlation coefficient (r) between conventional method and HApS$^{TM}$ method by a linear regression analysis, the correlation coefficients in total microorganism were 0.97767, 0.90712, and 0.95594 in pork, beef, and chicken samples, respectively. The correlation coefficients in coliform count were 0.82062, 0.94833, and 0.96839 in pork, beef and chicken samples, respectively. All the independent t-test on measurement values between conventional method and HApS$^{TM}$ method represented no significant differences in the means between two methods at the 0.05 of significance level($\alpha$=0.05). Based on the high correlation between HApS$^{TM}$ and the AOAC standard methods in the TAC and CC, it might be compatible to employ the HApS$^{TM}$ method to measure the microbial contamination in livestock products. HApS$^{TM}$ method was simpler and less time-consuming in sample preparation and procedures faster than the conventional method. These results suggested that the HApS$^{TM}$ method could be substitute for the conventional methods in the analysis of microbial contamination measurement in meat products.n meat products.

• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.1
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• pp.11-14
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• 1998

A toluene-oxidizing strain of Pseudomanas mendocina KR1 containing toluene-4-mono-oxygenase (TMO) completely degrades TCE with the addition of toluene as a co-substrate in aerobic condition. In order to construct in situ bioremediation system for TCE degradation without any growth-stimulating nutrients or toxic inducer such as toluene, we used the carbon-starvation promoter of Pseudomonas putida MK1 (Kim, Y. et al., J. bacteriol., 1995). Upon entry into the stationary phase due to the deprivation of nutrients, this promoter is strongly induced without further cell growth. The TMO gene cluster (4.5 kb) was spliced downstream of the carbon starvation promoter of Pseudomonas putida MK1, already cloned in pUC19. TMO under the carbon starvation promoter was not expressed in E. coli cells either in stationary phase or exponential phase. For TMO expression in Pseudomonas strains, tmo and carbon starvation promoter region were recloned into a modified broad-host range vector pMMB67HES which was made from pMMB67HE(8.9 kb) by deletion of tac promoter and lacIq (about 1.5 kb). Indigo was produced by TMO under the carbon starvation promoter in a Pseudomonas strain of post-exponential phase on M9 (0.2% glucose and 1mM indole) or LB. 18% of TCE was degraded in 14 hours after entering the stationary phase at the initial concentration of 6.6 ${\mu}$M in liquid phase.

• The Journal of Fisheries Business Administration
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• v.30 no.1
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• pp.31-50
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• 1999

The purpose of this paper is to offer policies or laws governing the future of Korean seafood markets in view of societal marketing concept. The environment surrounding those markets is changing quickly and constantly. The proposals in this paper are meant to help the Korean seafood market coping with this swiftly changing environment. This paper sorts this changing environment in terms of institutional side and secio-economical side. The institutional side involves the enforcement of international and domestic seafood trade standards, the increase of seafood importation, the adoption of optional seafood sales system, the openness of distribution market, and the adoption of TAC system. The secio-economical side involves the development of telecommunication and transportation, and the changing of seafood consumption pattern. The forecast about the future of seafood market could be classified into three fields, that is, the production field, the distribution field, and the consumption field of seafood. In the production field of seafood, the stabilization of supply of seafood and the production management oriented seafood market could be forecasted. In the distribution field, the formulating of enforced trade standards, the dispersion of marketing function among fisher, wholesaler and retailer, the development of marketing skills, and the promotion of marketing information system could be forecasted. Finally, in consumption field, the promotion of standardization and diversification, the appearance of intellectual consumers could be forecasted. This paper seeks to offer policies or laws fur the three categories of the seafood market-the government, the fisher, and the distributor-coping with the changing environment on the above three fields, thereby benefiting the consumer's long-term welfare. For the government, this paper suggests the construction of a Seafood Transaction Information Infrastructure, a Seafood Dealer License System, and a Seafood Safety Security System. For the fishers, this paper proposes an Eco-labelling System, a Sustainable Production System, and a Real Naming System in dealing seafood. Finally, for the distributors, this paper offers a Seafood Production Controlling System, a Nature-friendly Marketing System, and a Consumer-oriented Marketing System.

• The Journal of Fisheries Business Administration
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• v.34 no.2
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• pp.1-25
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• 2003

During the past 100 years, Korean fisheries management policies have enforced the license system, it is the most typical regulation system in Korean fisheries. Even now, it has caused resource management failure, it has also invited economic inefficiency and inequity. Accordingly, the Korean government has recently introduced the self-regulatory management system for fishermen with the TAC system. These systems are one of the most market-oriented regulation systems in fisheries management systems. It is defined as regulatory policy tools other than regulations with command and control approach. It is also called “alternatives” which is composed of regulatory alternatives and non-regulatory alternatives. The self-regulatory management system and the ITQ system are cooperative-based co-management between government and fishermen as an alternative management strategy ; it is gaining the increasing attention to improve the effectiveness of fisheries management in Korea. It is expected not only to positively improve function in fisheries regulation, but also to decrease inefficiency in fisheries policies. However, these systems have many problems. The monopolization of rent is one of the typical examples. As solutions for the problem in the aspect of fishery management, fisheries policy is need of approach to industrial organization. For instance, Contestable market theory is one of the good theoretical background, it mean that market is able to free entry and free exit. Thus, fisheries management carry out policies such as revitalization of the used market of capital goods in fisheries, organize of the self-regulatory management group. Conclusively, as the exploiters and managers, fishers and the government should put emphasis on improving economic efficiency so that fishery would grow as an industry that contributes to the increase of social welfare, and the change to that direction will be the only way for our fisheries to preserve its importance.

• Journal of Bio-Environment Control
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• v.25 no.4
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• pp.308-319
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• 2016

In order to set the outdoor weather conditions to be applied to the design standard of the greenhouse heating and cooling system, outdoor air temperature and heating degree-hour for heating design, dry bulb temperature, wet bulb temperature and solar irradiance for cooling design were analyzed and presented. For every region in Korea, we used thirty years from 1981 to 2010 hourly weather data for analysis, which is the current standard of climatological normal provided by KMA. Since the use of standard weather data is limited, design weather conditions were obtained using the entire weather data for 30 years, and the average value of the entire data period was presented as a design standard. The design weather data with exceedance probability of 1, 2.5, and 5% were analyzed by the TAC method, and we presented the distribution map with exceedance probability of 1% for heating and 2.5% for cooling which are recommended by design standards. The changes of maximum heating load, seasonal heating load and maximum cooling load were examined by regions, exceedance probabilities, and setpoint temperatures. The proposed outdoor design conditions can be used not only directly for the greenhouse heating and cooling design, but also for the reinforcement of heating and cooling facilities and the establishment of energy saving measures. Recently, due to the climate change, sweltering heat in summer and abnormal temperature in winter are occurring frequently, so we need to analyze weather data periodically and revise the design standard at least every 10 years cycle.

• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.13 no.2
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• pp.1-8
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• 2017

Outdoor design temperature is important for selecting proper capacity of heating and cooling systems of a building to implement indoor thermal comfort and save energy consumption. The purpose of this study is to investigate the change of South Korea outdoor design temperature according to the assigned period. When outdoor design temperature of 8 locations calculated with the latest weather data during 2008~2015 years using ASHRAE Bin method are compared to the standard temperature of Ministry of Land, Infrastructure and Transport which is widely used for designing South Korea air-conditioning system at present, the maximum temperature difference becomes $0.97^{\circ}C$ for cooling, and $1.94^{\circ}C$ for heating. Due to wide outdoor temperature variation, update of outdoor design temperature based on recent weather data is recommended.

• Archives of Pharmacal Research
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• v.27 no.3
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• pp.319-323
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• 2004

Cytochrome P450 (CYP) 3A4 is of great interest because of its important roles in the oxidation of numerous drugs and xenobiotics. HDJ-1, a molecular chaperone in human, is known to assist the correct folding of unfolded proteins. To achieve a high yield of recombinant human CYP3A4 in Escherichia coli, the CYP3A4 encoding gene was co-expressed with the chaperone HDJ-1, under the control of an inducible tac promoter in a bicistronic format. The levels of expression of the CYP3A4 in the bicistronic construct reached up to 715 nmol $(liter culture)^{-1}$ within 16 h at $37^{\circ}C$, which was about a 3.3-fold increase compared to that of the CYP3A4 alone without the HDJ-1. By co-expression with HDJ-1, the catalytic activity of CYP3A4 was also increased by -15-fold. The amount of activity increase was similar to that of the CYP production at the whole cell level. The present over-expression system may be useful for the rapid production of large amounts of active CYP3A4 in E. coli.