• Microbiology and Biotechnology Letters
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• v.15 no.6
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• pp.396-401
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• 1987

Protein productivities of a cloned gene ($\beta$-galactosidase) and the cultural performances of two recombinant Escherichia coli strains, which use different host-vector systems, were studied. E. coli JM109/pTBG10 strain which carries Tac promoter had higher protein productivity than E. coli MH3000 (pRKc1857)/pASI(lacZ) strain which carries pL promoter. Induction of protein syn-thesis was optimum at the initial-and mid-logarithmic growth phases for both strains. Oxygen demand was observed to be very high during the cloned gene expression, and could be alleviated to some extent through pH control. The ratio of specific growth rates of plasmid-harboring to plasmidfree cell, $\mu$+ /$\mu$-, of the high productivity strain was observed to be lower than that of the low productivity one. Plasmid stability was analyzed for 20-30 generations, and it was found that the traction of plasmid-harboring cells dropped to l0% level in about 25 generations for both strains when the cloned gene expression was induced.

• The Journal of Korean Medicine
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• v.28 no.1 s.69
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• pp.137-147
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• 2007

Objective : The objective of this study was to investigate the antioxidative effects of Carthami Flos extract. Methods : Total antioxidant status was examined by total antioxidant capacity(TAC) and total antioxidant response(TAR) against potent free radical reactions. The effect of Carthami Flos extract was examined far details of total phenolic content concentration at which 1,1-dipheny1-2-picrylhydrazyl(DPPH) radical scavenging activity was inhibited, the inhibitory effect on lipid peroxidation, and the effect on reactive oxygen species(ROS) generation. Results : TAC of Carthami Flos extract at the concentration of 5 mg/ml was 1.84 mM Trolox equivalent. 2. TAR of Carthami Flos extract, on the other hand, couldn't be determined due to interference from unidentified compounds. 3. Total phenolic content of Carthami Flos extract at the concentration of 5 mg/ml was 2.01 mM gallic acid equivalent. 4. Concentration of Carthami Flos extract at which DPPH radical scavenging activity was inhibited by 50% was 6.43 mg/ml as compared to 100% by Pyrogallol solution as a reference. 5. The inhibitory effect of the extract on lipid peroxidation was examined using rat liver mitochondria induced by FeS04/ascorbic acid. Carthami Flos extract at the concentration of 10 ms/ml slightly but significantly decreased TBARS concentration. The extract continued to prevent lipid peroxidation in a dose-dependent manner. 6. The effect of Carthami Flos extract on reactive oxygen species(ROS) generation was examined using a cell-free system induced by hydrogen peroxide/FeS04. Addition of 1 mg/ml of Carthami Flos extract significantly reduced dichlorofluorescein(DCF) fluorescence. Carthami Flos extract caused concentration-dependent attenuation of the increase in DCF fluorescence, indicating that the ektract significantly prevented ROS generation in vitro. Conclusion: : Antioxidant efffcts of Carffami ffor extract seem to be due, at least in part, to the prevention offree radical-induced oxidation, fellowed by inhibition of lipid peroxidation.

• The Journal of Korean Medicine
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• v.28 no.1 s.69
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• pp.148-158
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• 2007

Objective : The objective of this study was to investigate the antioxidant effects of Mori Folium extract. Methods Total antioxidant status was examined by total antioxidant capacity(TAC) and total antioxidant response(TAR) against potent free radical reactions. The effect of Mori Folium extract was examined by measuring total phenolic content, concentration at which 1,1-dipheny1-2-picrylhydrazyl(DPPH) radical scavenging activity was inhibited, inhibitory effect on lipid peroxidation, and the effect on reactive oxygen species(ROS) generation. Results : 1. TAC and TAR of Mori Folium extract at the concentration of 5 mg/ml were 1.61 and 1.24 mM Trolox equivalents, respectively. 2. Total phenolic content of Mori Folium extract at the concentration of 5 mg/Ml was 1.70 mM gallic acid equivalent. 3. Concentration of Mori Folium extract at which DPPH radical scavenging activity was inhibited by 50% was 2.29 m9/m4 as compared to 100% by Pyrogallol solution as a reference. 4. The inhibitory effect of the extract on lipid peroxidation was examined using rat liver mitochondria induced by FeSO$_4$/ascorbic acid. Mori Folium extract at the concentration of 10 mg/ml significantly decreased thiobarbituric acid reactive substances(TBARS) concentration. The extract prevented lipid peroxidation in a dose-dependent 5. The effect of Mori Folium extract on reactive oxygen species(ROS) generation was examined using a celt-free system induced by hydrogen peroxide FeSO$_4$. Addition of 1 mg/ml of Mori Folium extract significantly reduced dichlorofluorescein(DCf) fluorescence. The extract caused concentration-dependent attenuation of the increase in DCF fluorescence, indicating that the extract significantly prevented ROS generation in vitro. Conclusion ; The antioxidant effects of Mori Folium extract seem to be due, at least in part, to the prevention offree radical-induced oxidation, fllowed by inhibition of lipid peroxidation.

• Proceedings of the Korean Society of Medical Physics Conference
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• 1999.11a
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• pp.414-415
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• 1999

• Proceedings of the KIEE Conference
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• 1996.11a
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• pp.106-108
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• 1996

This paper presents the Implementation and performance simulation of HSGS(High Speed Ground Switch)-Adaptive scheme for EHV transmission. EMTP/TACS(Transient Analysis of Control System) functions are combined and used for the implementation and simulation. The simulation results are compared with those for the conventional reclosing scheme and discussed.

• The Journal of Internal Korean Medicine
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• v.32 no.2
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• pp.243-258
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• 2011

Objectives : This study investigated the hypolipidemic and antioxidant effects of Curcuma radix using a rat model induced by poloxamer 407 injection. Methods : Serum lipid parameters and oxidative stress-associated biomarkers were determined. Additionally, hepatic cholesterol and triglyceride as well as lipid metabolism-associated gene expressions were observed in hepatic tissue. Results : 1. Curcuma radix ameliorated elevation of serum cholesterol, triglyceride, LDL-cholesterol, MDA, hepatic cholesterol level, and reduction of serum TAC, SOD, GSH, GSH-reductase level. 2. Curcuma radix augmented up-regulated ACAT gene expression. 3. Curcuma radix almost completely ameliorated down-regulated CYP-7A1 but up-regulated HMG-CoA gene expression. Conclusions : The hypolipidemic and antioxidant properties of Curcuma radix were evidenced. This study provides a scientific basis for the clinical application of Curcuma radix and development of hypolipidemics using this herb in the future.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.28 no.5
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• pp.277-284
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• 2015

The humans are under attack involving the hazardous environment and pathogen/biotoxin aerosol that is realistic concerned. A portable, fast, reliable, and cheap Pathogen and Biotoxin Aerosol threat Detection(PBAD) trigger is an important technology for detect-to-protect and detect-to-treat system because the man-made biological terror is a fast and lethal infection. The ultraviolet C(UVC) wavelengths light source is key issue for PBAD that is sensitive because of strong fluorescence cross section from fluorescent amino acids in proteins such as tryptophan and tyrosine. The UVC-light emitting diode(LED) is emerging light source technology as alternative to laser or lamps as they offer several advantages. This paper discussed about the design consideration of UVC-LED for the PBAD system. The UVC-LED and PBAD technology, currently available or in development, are also discussed.

• Korean System Dynamics Review
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• v.5 no.2
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• pp.67-88
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• 2004

This paper attempts to explore the generic pitfalls of the traditional number-crunching methods adopted thus far for the forecast of newly emerging market trends, and present an alternative by introducingsystems thinking to the portable Internet service market as an example, followed by its rationale as a new tool for forecasting and some reasoning about why traditional methods are no longer appropriate. Most adoption models in general to forecast market trends have several limitations due to theirbasic assumptions and prospective. First, they fail to capture dynamic interactions among the factors involved over time, with implicit assumptions of 'unilateral causality' in that each factor contributes as a cause to the effect, i.e., causality runs one way; each factor acts independently the weighting factor of each is fixed, etc. Second, the number-crunching models have no way of taking into account the impact of delayed feedback often caused by introducing new policies and legislative changes on the whole system under investigation. Third, there is not a way to reflect the effect of competition by players.

• The Journal of Information Systems
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• v.19 no.1
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• pp.79-96
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• 2010

The organizations in the competitive business environment are under pressure of handling internal management processes as well as external supply chain issues. In addition, the paradigm of supply chain management (SCM) has been shifted from static to dynamic problems. For the efficient SCM, organizations should be a RTE (Real Time Enterprises) that views entire supply chains as a single entity. In this paper, the agent system that makes RTE and dynamic supply chains possible is developed. Then, the developed agent system is verified in the TAC SCM(Trading Agent Competition Supply Chain Management) experimental environment and a dashboard is also developed for monitoring management activities of supply chains. The contributions of this paper are as followings. First, unlike the previous agent systems, the suggested agent system in this paper provides autonomy and scalability using SOA. Second, this suggested model for dynamic environment can be applied for the development of software supporting a SCM.

• Journal of Fisheries and Marine Sciences Education
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• v.12 no.1
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• pp.98-121
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• 2000

The Korean fishery encountered a difficulty situation, due to the new regime of the Exclusive Economic Zone (EEZ) and the decreasing fisheries resources in Korean waters. In addition, the coastal areas are deteriorated by industrial wastes, sewage, farming wastes and pollution from aquaculture. In this situation, it is necessary to study the TAC (total allowable catch) - based management system, the development of fishing gears and appropriate fishing methods for stock conservation, and the automation system of fishing gears for improving the efficiency of fisheries. The objective of this study is to look for an appropriate system in marine production and resource management under the new UNCLOS (United National Convention for the Law of the Sea) regime for subjects in fishing gears and methods, production system, and information, and fisheries resources management. The results of this study could be used as scientific information to maintain and develop the Korean fisheries and to establish fisheries policy for the management of fisheries resources in Korean waters.