The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Lipid-lowering and Antioxidant Effects of Curcuma Radix in Poloxamer 407-induced Hyperlipidemia Model Rat Models

  • Park, So-Ae (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Jo, Hyun-Kyung (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Yoo, Ho-Ryong (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Kim, Yoon-Sik (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Seol, In-Chon (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • An, Joung-Jo (Division of Circulatory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University)
  • Published : 2011.06.30
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Abstract

Objectives : This study investigated the hypolipidemic and antioxidant effects of Curcuma radix using a rat model induced by poloxamer 407 injection. Methods : Serum lipid parameters and oxidative stress-associated biomarkers were determined. Additionally, hepatic cholesterol and triglyceride as well as lipid metabolism-associated gene expressions were observed in hepatic tissue. Results : 1. Curcuma radix ameliorated elevation of serum cholesterol, triglyceride, LDL-cholesterol, MDA, hepatic cholesterol level, and reduction of serum TAC, SOD, GSH, GSH-reductase level. 2. Curcuma radix augmented up-regulated ACAT gene expression. 3. Curcuma radix almost completely ameliorated down-regulated CYP-7A1 but up-regulated HMG-CoA gene expression. Conclusions : The hypolipidemic and antioxidant properties of Curcuma radix were evidenced. This study provides a scientific basis for the clinical application of Curcuma radix and development of hypolipidemics using this herb in the future.

Keywords

References

  1. Huang TL, Chen JF. Cholesterol and lipids in depression: stress, hypothalamo-pituitary-adrenocortical axis, and inflammation and immunity. Adv Clin Chem 2005;39:81-105.
  2. Keenan JM. Treatment of patients with lipid disorders in the primary care setting: new treatment guidelines and their implications. South Med J 2003;96(3):266-75. https://doi.org/10.1097/01.SMJ.0000061513.59902.6A
  3. Kelly RB. Diet and exercise in the management of hyperlipidemia. Am Fam Physician 2010;81(9):1097-102.
  4. Huang TL, Chen JF. Cholesterol and lipids in depression: stress, hypothalamo-pituitary-adrenocortical axis, and inflammation/immunity. Adv Clin Chem 2005;39:81-105.
  5. Kashani A, Sallam T, Bheemreddy S, Mann DL, Wang Y, Foody JM. Review of side-effect profile of combination ezetimibe and statin therapy in randomized clinical trials. Am J Cardiol 2008;101(11):1606-13. https://doi.org/10.1016/j.amjcard.2008.01.041
  6. Mukhtar RY, Reckless JP. Statin-induced myositis: a commonly encountered or rare side effect. Curr Opin Lipidol 2005;16(6):640-7.
  7. Araujo FB, Barbosa DS, Hsin CY, Maranhao RC, Abdalla DS. Evaluation of oxidative stress in patients with hyperlipidemia. Atherosclerosis 1995;117(1):61-71. https://doi.org/10.1016/0021-9150(94)05558-Z
  8. Yang RL, Shi YH, Hao G, Li W, Le GW. Increasing Oxidative Stress with Progressive Hyperlipidemia in Human: Relation between Malondialdehyde and Atherogenic Index. J Clin Biochem Nutr 2008;43(3):154-8. https://doi.org/10.3164/jcbn.2008044
  9. Racek J, Rusnakova H, Trefil L, Siala KK. The influence of folate and antioxidants on homocysteine levels and oxidative stress in patients with hyperlipidemia and hyperhomocysteinemia. Physiol Res 2005;54(1):87-95.
  10. Sefi M, Fetoui H, Makni M, Zeghal N. Mitigating effects of antioxidant properties of Artemisia campestris leaf extract on hyperlipidemia, advanced glycation end products and oxidative stress in alloxan-induced diabetic rats. Food Chem Toxicol 2010;48(7):1986-93. https://doi.org/10.1016/j.fct.2010.05.005
  11. Ponce-Canchihuaman JC, Perez-Mendez O, Hernandez-Munoz R, Torres-Duran PV, Juarez -Oropeza MA. Protective effects of Spirulina maxima on hyperlipidemia and oxidative-stress induced by lead acetate in the liver and kidney. Lipids Health Dis 2010;9:35. https://doi.org/10.1186/1476-511X-9-35
  12. Oh HI, Park HB, Ju MS, Jung SY, Oh MS. Comparative Study of Anti-oxidant and Anti -inflammatory Activities between Curcumae Longae Radix and Curcumae Longae Rhizoma. J of herbology 2010;25(1):83-91.
  13. Seo EA, Chung HT, Ko KH, Kwon KB. Cytoprotective Effects of Radix Curcumae Aromaticae in Human Umbilical Vein Endothelial Cells. J. of Oriental Pathology 2004;18(6):1805-9.
  14. Kambayashi Y, Binh NT, W Asakura H, Hibino Y, Hitomi Y, Nakamura H, et al. Efficient assay for total antioxidant capacity in human plasma using a 96-well microplate. J Clin Biochem Nutr 2009;44(1):46-51. https://doi.org/10.3164/jcbn.08-162
  15. Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978;186(1):271-8.
  16. Hayashi I, Morishita Y, Imai K, Nakamura M, Nakachi K, Hayashi T. High-throughput spectrophotometric assay of reactive oxygen species in serum. Mutat Res 2007;631(1):55-61. https://doi.org/10.1016/j.mrgentox.2007.04.006
  17. Ellman GL, Evans JC. The ionization of cysteine. Biochem Biophys Acta 1959;33:574-6. https://doi.org/10.1016/0006-3002(59)90157-X
  18. Worthington DJ, Rosemeyer MA. Human glutathione reductase: purification of the crystalline enzyme from erythrocytes. Eur J Biochem 1974;48(1):167-77. https://doi.org/10.1111/j.1432-1033.1974.tb03754.x
  19. Brites FD, Bonavita CD, Cloës M, Yael MJ, Frunchart JC, Castro GR, et al. VLDL compositional changes and plasma levels of triglycerides and high density lipoprotein. Clin Chim Acta 1998;269(2):107-24. https://doi.org/10.1016/S0009-8981(97)00193-9
  20. Sirtori CR, Fumagalli R. LDL-cholesterol lowering or HDL-cholesterol raising for cardiovascular prevention. A lesson from cholesterol turnover studies and others. Atherosclerosis 2006;186(1):1-11. https://doi.org/10.1016/j.atherosclerosis.2005.10.024
  21. Walsh JM, Pignone M. Drug treatment of hyperlipidemia in women. JAMA 2004;291(18):2243-52. https://doi.org/10.1001/jama.291.18.2243
  22. Farnier M, Davignon J. Current and future treatment of hyperlipidemia: the role of statins. Am J Cardiol 1998;82(4B):3J-10J.
  23. Sinzinger H. Side effects of statins--prevalence, relevance, consequences. Wien Klin Wochenschr 2002;114(21-22):887-8.
  24. Farnier M, Davignon J. Current and future treatment of hyperlipidemia: the role of statins. Am J Cardiol 1998;82(4B):3J-10J.
  25. Steinmetz KL, Schonder KS. Colesevelam: potential uses for the newest bile resin. Cardiovasc Drug Rev 2005;23(1):15-30.
  26. Ahmed SM, Clasen ME, Donnelly JE. Management of dyslipidemia in adults. Am Fam Physician 1998;57(9):2192-204.
  27. Shin WT, Heo JE, Kim TH, Hong SH, Kwon OS, Kim KT, et al. Clinical effect of Dodam -tang on hyperlipidemia patients. Korean J. Orient. Int. Med 2007;fal:113-8.
  28. Kim BC, Lee EJ, Park CS, Park CG. The experimental study on the effects of Dansamyeum on hyperlipidemia. Korean J. Orient. Int. Med 2000;21:126-34.
  29. Kim H, An JJ, Jo HK, Yoo HR, Seol IC, Kim YS. Experimental Study on the Effects of GamiSamgieum(SGMX) on Hyperlipidemia. J Korean Oriental Med 2009;30(3):86-97.
  30. Gong TH, Jeong JC. Effects of Ojung-hwan on Blood Glucose, Hyperlipidemia, Polyol Pathway and Antioxidative Mechanism in Ob/ob Mouse. J Korean Oriental Med 2007;28(3):57-69.
  31. Shin HS, Kim GY, Seo IB, Kim HH. Preventive Effects of Typhae Pollen on the Diet-induced Hyperlipidemia in Rats. J Korean Oriental Med 2003;24(2):32-9.
  32. Johnston TP. Poloxamer 407 as a general lipase inhibitor: its implications in lipid metabolism and atheroma formation in C57BL/6 mice. J Pharm Pharmacol 2010;62(12):1807-12. https://doi.org/10.1111/j.2042-7158.2010.01138.x
  33. Pfizer 2008 Annual Report. Pfizer. http://media.pfizer.com/files/annualreport/2008/annual/review2008.pdf.
  34. Kim TH, Son YK, Hwang KH, Kim MH. Effects of Angelica keiskei Koidzumi and Turmeric Extract Supplementation on Serum Lipid Parameters in Hypercholesterolemic Diet or P-407-Induced Hyperlipidemic Rats. J Food Science and Nutrition 2008;37(6):708-13.
  35. Ju YS, Yeon JY, Kim MH, Kang MH, Kim TH, Son YK, et al. The Effects of Angelica keiskei Koidzumi and Turmeric Extract Supplementation on the Blood Lipids, and Antioxidant and Inflammatory Markers in Hypercholesterolemic Adults in Korea. J Food Science and Nutrition 2009;22(4):517-25.
  36. Suagee JK, Corl BA, Crisman MV, Wearn JG, McCutcheon LJ, Geor RJ. De novo fatty acid synthesis and NADPH generation in equine adipose and liver tissue. Comp Biochem Physiol B Biochem Mol Biol 2010;155(3):322-6. https://doi.org/10.1016/j.cbpb.2009.11.019
  37. Xie X, Watanabe K, Wojcicki WA, Wang CC, Tang Y. Biosynthesis of lovastatin analogs with a broadly specific acyltransferase. Chem Biol 2006;13(11):1161-9. https://doi.org/10.1016/j.chembiol.2006.09.008
  38. Myant NB, Mitopoulos KA. cholesterol 7 alpha -hydroxylase. J. Lipid Res 1977;18(2):135-53.
  39. Chiang JYL, Miller WF, Lin GM. Regulation of cholesterol 7 alpha-hydroxylase in the liver. J Biol Chem 1990;265:3889-97.
  40. Roth BD. ACAT inhibitors: evolution from cholesterol-absorption inhibitors to antiatherosclerotic agents. DDT 1998;3(1):19-25.
  41. Keisuke K, Toshiya T, Rina A, Kouki T, Toshinobu M, Notio N, et al. Structure of the human acyl-CoA: cholesterol acyltransferase -2(ACAT-2) gene and its relation to dyslipidemia. Biochim Biophys Acta 2001;1531(3):230-40. https://doi.org/10.1016/S1388-1981(01)00106-8
  42. Magder S. Reactive oxygen species: toxic molecules or spark of life? Crit Care 2006;10(1):208. https://doi.org/10.1186/cc3992
  43. Kurata M, Suzuki M, Agar NS. Antioxidant systems and erythrocyte life-span in mammals. Comp Biochem Physiol B 1993;106(3):477-87. https://doi.org/10.1016/0305-0491(93)90121-K
  44. Parke DV, Parke AL. Chemical-induced inflammation and inflammatory diseases. Int J Occup Med Environ Health 1996;9(3):211-7.
  45. Van Deel ED, Lu Z, Xu X, Zhu G, Hu X,Oury TD, et al. Extracellular superoxide dismutase protects the heart against oxidative stress and hypertrophy after myocardial infarction. Free Radic Biol Med 2008;44(7):1305-13. https://doi.org/10.1016/j.freeradbiomed.2007.12.007
  46. Rai B, Kaur J, Jacobs R, Singh J. Possible action mechanism for curcumin in pre-cancerous lesions based on serum and salivary markers of oxidative stress. J Oral Sci 2010;52(2):251-6. https://doi.org/10.2334/josnusd.52.251
  47. Sompamit K, Kukongviriyapan U, Nakmareong S, Pannangpetch P, Kukongviriyapan V. Curcumin improves vascular function and alleviates oxidative stress in non-lethal lipopolysaccharide-induced endotoxaemia in mice. Eur J Pharmacol 2009;616(1-3):192-9. https://doi.org/10.1016/j.ejphar.2009.06.014