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Effects of a Microbial Enzyme Supplementation on the Performance of Laying Hens Fed Diets Containing Different Levels of Wheat

  • Um, J.S.;Ahn, S.H.;Paik, I.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.6
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    • pp.702-707
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    • 1998
  • An experiment was conducted to evaluate the effect of a microbial enzyme (Roxazyme-$G^{(R)}$), a multicarbohydrases preparation, supplementation to the wheat-based layer diets. Diets were formulated to include different levels of wheat replacing yellow corn on isocaloric and isonitrogenous basis. The energy value of wheat in the enzyme supplemented diets was adjusted (spec-modified) to have 5% more ME than the wheat in diets without enzyme. A total of 864 Hy-$Line^{(R)}$ brown layers were assigned to 4 dietary treatments: 10% wheat (T1), 25% wheat (T2), 25% wheat (spec-modified)+ 0.01 % Roxazyme-$G^{(R)}$ (T3), and all wheat (spec-modified)+0.01% Roxazyme-$G^{(R)}$ (T4). Hen-day egg productions of T1 and T4 were significantly (p < 0.05) greater than that of T2 but not different from T3. Hen-housed egg production of T4 was significantly (p < 0.01) greater than those of T1 and T3 but not different from T2. Egg weights of T1 and T2 were significantly (p < 0.0 1) greater than that of T4. Feed consumption of T2 was significantly (p < 0.01) lower than other treatments. Feed conversion ratio (feed/egg mass) was not significantly different among treatments. Eggshell thickness of T1 was significantly (p < 0.01) greater than other treatments but ratio of broken eggs was not significantly different among treatments. Haugh unit of T4 was significantly greater (p < 0.05) than that of T2. Egg yolk color was significantly (p < 0.01) influenced by treatments in which enzyme treatment potentiated the yolk pigmentation. It was concluded that a multi-carbohydrases supplementation enables complete replacement of yellow com with wheat without loss of productivity and major egg quality parameters.

Activation of Pro-Apoptotic Multidomain Bcl-2 Family Member Bak and Mitochondria-Dependent Caspase Cascade are Involved in p-Coumaric Acid-Induced Apoptosis in Human Jurkat T Cells (p-Coumaric acid에 의해 유도되는 인체 Jurkat T 세포의 에폽토시스 기전)

  • Lee, Je-Won;Kim, Young-Ho
    • Journal of Life Science
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    • v.21 no.12
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    • pp.1678-1688
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    • 2011
  • The apoptogenic effect of p-coumaric acid, a phenolic acid found in various edible plants, on human acute leukemia Jurkat T cells was investigated. Exposure of Jurkat T cells to p-coumaric acid (50-$150{\mu}M$) caused cytotoxicity and TdT-mediated dUTP nick-end labeling (TUNEL)-positive apoptotic DNA fragmentation along with Bak activation, ${\Delta}{\psi}m$ loss, activation of caspase-9, -3, -7, and -8, and PARP degradation in a dose-dependent manner. However,these apoptotic events were completely abrogated in Jurkat T cells overexpressing Bcl-2.Under these conditions, necrosis was not accompanied. Pretreatment of the cells with the pan-caspase inhibitor (z-VAD-fmk) could prevent p-coumaric acid-induced sub-$G_1$ peak representing apoptotic cells, whereas it failed to block ${\Delta}{\psi}m$ loss, indicating that the activation of caspase cascade was prerequisite for p-coumaric acid-induced apoptosis as a downstream event of ${\Delta}{\psi}m$ loss. FADD- and caspase-8-positive wild-type Jurkat T cell clone A3, FADD-deficient Jurkat T cell clone I2.1, and caspase-8-deficient Jurkat T cell clone I9.2 exhibited similar susceptibilities to the cytotoxicity of p-coumaric acid, excluding an involvement of Fas/FasL system in triggering the apoptosis. The apoptogenic activity of p-coumaric acid is more potent in malignant Jurkat T cells than in normal human peripheral T cells. Together, these results demonstrated that p-coumaric acid-induced apoptogenic activity in Jurkat T cellswas mediated by Bak activation, ${\Delta}{\psi}m$ loss, and subsequent activation of multiple caspases such as caspase-9, -3, -7, and-8, and PARP degradation, which could be regulated by anti-apoptotic protein Bcl-2.

Factors Affecting the Satisfaction of Simulation Education about Emergency Delivery for Paramedic Students (응급구조학과 학생의 응급분만 시뮬레이션 교육 만족도 영향요인)

  • Baek, Mi-Lye
    • Fire Science and Engineering
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    • v.28 no.5
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    • pp.98-103
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    • 2014
  • This study investigated the confidence, satisfaction, and psychological safety and identified factors affecting the satisfaction of 41 paramedic students. Data, which consists of pre and post-confidence items on visual analogue scale, 21 satisfaction items and 10 psychological safety items was analyzed using SPSS WIN 21.0 program. After the simulation education, the students were told to write what they felt about the education. Then the students' comments, which were categorized as effectiveness and difficulty, were analyzed. Satisfaction of general characteristics showed a significant difference in age (t = -2.22, p = 0.03) and psychological safety of general characteristics showed a significant difference in gender (t = 3.70, p = 0.00) and age (t = -3.65, p = 0.00). Pre-confidence is low (4.37); however, post-confidence increased to 6.22. It showed a significant difference in pre and post-confidence (t = -5.878, p = 0.000). Satisfaction was correlated with psychological safety (r = 0.516, p = 0.001). The variables affecting satisfaction were psychological safety (t = 3.021, p = 0.005) and post-confidence (t = 1.936, p = 0.061). These variables accounted for 27.6% of the satisfaction according to a regression analysis. The simulation education may be the new effective paramedic education strategy of emergency childbirth. Repetitive simulation learning with further development of various scenarios, more education time, and more faculties will be able to train field compatible paramedics.

Image of nurse and nursing professionalism perceived of nursing students before and after their first clinical practice in the COVID-19 pandemic (코로나19(COVID-19) 유행 시 첫 임상실습 전·후 간호대학생의 간호사이미지와 간호전문직관의 차이)

  • Lee, Oi-Sun;Lim, Jung-Hye
    • Journal of Digital Convergence
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    • v.19 no.5
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    • pp.479-488
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    • 2021
  • This study was attempted to investigate the effect of the first clinical practice on the image of nurse and nursing professionalism of nursing students in the COVID-19 situation. The data were collected from 36 nursing college students using a structured questionnaire from June to July 2020 and analyzed by PASW 22.0. After the first clinical practice in the COVID-19 pandemic, the nurse image (t=-2.219, p=.034) and nursing professionalism(t=-3.444, p=.002) increased significantly compared to before practice. Social image (t=-3.113, p=.004), self-concept of the profession(t=-2.442, p=.021), social awareness(t=-3.829, p=.001) and professionalism of nursing(t=-2.299, p=.028) significantly increased after the first clinical practice. After the first clinical practice, nursing professionalism showed a significant positive correlation with image of nurse(r=.831, p<.001). The attitude of nurses to cope with COVID-19 positively influenced nursing students' nurse image and nursing professionalism. Therefore, it is expected that will be used as basic data for preparing educational strategies after COVID-19.

Factors Affecting on Suicidal Ideation in Community Dwelling Elders (지역사회 노인의 자살생각에 영향을 미치는 요인)

  • Park, Keum-Sook;Kim, Young-Hee;Lee, Kyoung-Wan;Lyu, Yeoung-Su;Jeong, Heon-Young
    • Journal of Oriental Neuropsychiatry
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    • v.26 no.1
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    • pp.39-48
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    • 2015
  • Objectives: This study was conducted to analyze the hwa-byung, self-perceived health, life-stress, social support, depression, and factors affecting on suicidal ideation in community dwelling elders. Methods: This study was a cross-sectional descriptive study. The subjects were 165 elderly in I city. The data was collected from November $21^{st}$ to December $5^{th}$ of 2014 by using structured questionnaire. T-test, Pearson's correlation coefficients, and stepwise multiple regression were performed by SPSS/PC 17.0. Results: There were more females than males in hwa-byung (t=.-3.068, p=.003). In the visit parent, more than once a month was a significantly higher in social support (t=.3.333, p=.001). Hwa-byung (t=.-2.577, p=.011), life-stress (t=.-3.800, p=.001), depression (t=.-2.776, p=.006), and suicidal ideation (t=.-4.316, p<.001) were shown to be high in the elders with lower level of living. Subjects with health insurance had high self-perceived health (t=-3.800, p=.001) and low hwa-byung (t=-4.102, p<.001), life-stress (t=-4.482, p<.001), depression (t=-5.296, p<.001), and suicidal ideation (t=.-2.152, p=.036), according to the type of insurance. In the correlation, sleeping hours (r=-.180, p=.020), self-perceived health (r=-.364, p,.001), and suicidal ideation had negative correlation; hwa-byung (r=.404, p<.001), life-stress (r=.300, p<.001), social support (r=-.310, p<.001), depression (r=.423, p<.001) and suicidal ideation had positive correlation. Factors affecting the suicidal ideation in community dwelling elders were depression (${\beta}$=.42, p<.001), hwa-byung (${\beta}$=.23, p=.004), social support (${\beta}$=-.18, p=.012), and level of living (${\beta}$=.24, p=.001). Discussion: For the prevention of elderly suicide, it is necessary to care for psychosocial status and solve economic difficulties through social support.

Study on the Silage from Rice Straw and Persicaria Thunbergii (볏짚과 고마리(Persicaria Thunbergii Gross var. Coreana Gross ex Nakai)의 혼합Silage 제조에 관한 연구)

  • Kim, Yong-Kook
    • Korean Journal of Agricultural Science
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    • v.5 no.2
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    • pp.115-119
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    • 1978
  • To improve the feed value of rice straw, Tongil type rice straw and Persicaria Thunbergii Gross var. coreana Gross ex Nakai were mixed in the ratios of 10 : 90 (T1), 20 : 80 (T2), 30 : 70 (T3) and 40 : 60 (T4) in fresh weight and ensiled for 6 weeks. The chemical composition of fresh materials and silage, the organic acid contents and pH in silage were determined. And the results obtained were summarized as follow: 1. The crude protein content in rice straw was 8.18% of DM basis and that in Persicaria Thunbergii was 14.47%. And the nitrogen-free extract content in rice straw was 44.66% and.. that in Persicaria Thunbergii was 51.13%. 2. The optimum moisture contents were appeared in T2 as 72.12% and T3 as 66.43%. Considerably high crude protein contents were determined from all of treatments. 3. The pH of silage was varied from pH 3.8 of T1 to pH 4.5 of T2. And Total organic acid contents were increased from T1 to T4, but the percentage of lactic acid contents to total acid contents were decreased. 4. It was concluded that good quality silage could be made from rice straw with Persicaria Thunbergii and the best ratios of rice straw: Persicaria Thunbergii were between 20 : 80 and 30 : 70 in fresh weight.

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우리나라 인공호의 부영양화 평가 및 예측에 관한 연구

  • 김재윤
    • Journal of Environmental Science International
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    • v.7 no.4
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    • pp.441-450
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    • 1998
  • The purpose of this study is to evaluate and to predict of eutrophication in lakes by using VollenweiderGECD model and total phosphorus concentration and inflow rate which were measurded in 1993-1996. The results of study was as follows. The annual total phosphorus loading from the watershed was calculated to be 181-195tP /yr at lake Soyang, 591-680tP/yr at lake Chungju, 420-466tP/yr at lake Taechong, 229-278tP/yr at lake Andong, 103-106tP/yr at lake Hapchon, 57-59tP/yr at lake Imha, 194-244tP/yr at lake Namgang, 8386tP /yr at lake Chuam, 99-109tP /yr at lake Somjin. These are discharged, for the most parts, from population and ftshfarm facility. TP loading on the surface area at lake Soyang was 3.0lgP/$m^2$/yr, 2.82gP/$m^2$/yr, 2.84gP/$m^2$/yr, 3. 03gP/$m^2$/yr, at lake Chungju 7.91gP/$m^2$/yr, 6.87gP/$m^2$/yr, 7.38gP/$m^2$/yr, 7.l8gP/$m^2$/yr, at lake Taechong 6.7lgP/$m^2$/yr, 7.25gP/$m^2$/yr, 7.24gP/$m^2$/yr, 6.53gP/$m^2$/yr and TP loading on the surface area of Nakdong river basin, that is, lake Andong, Imha, Hapchon and Namgang were 5.39gP/$m^2$/yr, 4.47gP/$m^2$/yr, 4. 56gP/$m^2$/yr, 4.45gP/$m^2$/yr and 2.20gP/$m^2$/yr, 2.23gP/$m^2$/yr, 2.24gP/$m^2$/yr, 2.l7gP/$m^2$/yr and 4.50gP/$m^2$/ yr, 4.50gP/$m^2$/yr, 4.54gP/$m^2$/yr, 4.43gP/$m^2$/yr and 8.25gP/$m^2$/yr, 8.48gP/$m^2$/yr, 8.48gP/$m^2$/yr, 10. 39gP/$m^2$/yr respectively. Also those of lake Chuam was 2.51gP/$m^2$/yr, 2.61gP/$m^2$/yr, 2.52gP/$m^2$/yr, 2. 54gP/$m^2$/yr and TP loading on the surface area at lake Somjin was analysed 4.09gP/$m^2$/yr, 4.l0gP/$m^2$/yr, 3.98gP/$m^2$/yr,3.73gP/$m^2$/yr. The tropic states of nine lakes can be assessed as eutrophy because phosphorus loading exceeds the critical phosphorus loading by Vollenwelder-GECD model.

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Meat Quality and Sensory Properties of Korean Native Black Goat by Different Castration Age (거세시기에 따른 재래흑염소 육의 육질 및 관능적 특성)

  • Kim, Byung-Ki;Hwang, Eun-Gyeong;Kim, Su-Min
    • Food Science of Animal Resources
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    • v.30 no.3
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    • pp.419-426
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    • 2010
  • This study was conducted to investigate the effect of different castration ages on meat quality and sensory properties of Korean native black goats over 410 days. For the experiment, 32 heads of goat (eight heads/4 treatment) were subjected to either a control (5 month non-castration), T1 (7 month castration), T2 (5 month castration) or T3 (3 month castration). The total weight gain for Korean native black goats was highest in the T2 group after feeding for 410 days and the weight gain/day tended to be similar to the total weight gain. The total feeding amounts were lowest (410.82 kg) in T3; however, the feed intake ratio was 16.39 in T2, indicating that it had the best feed efficiency among groups. The cooking loss and drip loss of the Korean native black goats was highest in the control, being 35.53% and 2.08%, respectively (p<0.05), while the total cholesterol of the treatments was higher than that of the control (p<0.05). Moreover, the overall sensory evaluation of the treatment groups was low, indicating that there was more meat flavor when compared to the controls in terms of juiciness, tenderness, flavor, texture, black goat off-flavor and overall evaluation (p<0.05). T2 was found to have the best meat flavor upon sensory evaluation. Additionally, the meat color of the control showed the highest $L^*$ value and Hue value, while T3 showed the highest $a^*$ value (3.61) and T2 showed the highest $b^*$ value and Chroma. The composition of fatty acids was 53.76% oleic acid in T2, while the amounts of Mono-unsaturated fatty acid (MUFA) were highest in T1 and T2 (p<0.05). As a result, the MUFA/SFA ratios of T1 and T2 were higher than those of the control (p<0.05). In conclusion, it is most advantageous to castrate Korean native black goats at the age of 5 months for the best performance and meat quality.

Effect of Garlic and Onion Juice Addition on the Lipid Oxidation, Total Plate Counts and Residual Nitrite Contents of Emulsified Sausage during Cold Storage (마늘즙 또는 양파즙 첨가가 유화형 소시지의 저장 중 지방 산화, 총 미생물수 및 아질산염잔존량에 미치는 영향)

  • Park, Woong-Yeoul;Kim, Young-Jik
    • Food Science of Animal Resources
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    • v.29 no.5
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    • pp.612-618
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    • 2009
  • The objective of this study was to determine the antioxidant and antimicrobial effects of garlic juice and onion juice in emulsified sausage during cold storage. The sausages were into five groups: control, 1% garlic juice (T1), 3% garlic juice (T2), 1% onion juice (T3), and 3% onion juice (T4). Each sausage type was tested in triplicate and assigned to one of four storage periods: 0, 7, 14 and 21 days. As storage time increased, the presence of garlic juice and onion juice resulted in decreased pH, residual nitrite value, and increased peroxide value, TBARS (thiobarbituric acid reactive substance) values, and total plate counts. The pH value, peroxide value, TBARS, residual nitrite and total plate counts were significantly decreased by the addition of garlic and onion juice relative to the control (p<0.05). Especially, T2 was significantly (p<0.05) more effective in delaying lipid oxidation compared to the other treatment groups. However, no significant difference (p<0.05) was found in total plate counts among all the formulations on day 0. Also, the use of garlic juice resulted in much better antioxidant and antimicrobial effects than the use of onion juice and the control. In conclusion, this study demonstrates that the addition of 3% garlic juice (T2) to emulsified sausages tended to improve antioxidative and antimicrobial effects during storage relative to the other treatment groups.

A Study of the Relationship between Self Concept and Depression of Middle-Aged Women (중년여성의 자아개념과 우울에 관한 연구)

  • Kim, Jung-Youb
    • Women's Health Nursing
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    • v.3 no.2
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    • pp.113-128
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    • 1997
  • The purpose of this study was to investigate the relation between self concept and depression of middle-age women. Data were collected by a questionnaire from June 23 to July 3, 1997. The subject for this study were 138 women between 40-60 years of age living in Seoul and Incheon. The instruments used for this study were the self concept scale developed by Rosenberg and Choi, Jung-Hun and the depression scale developed by Rockliff and the investigator. The data were analyzed by descriptive statistics, t-test, ANOVA, Pearson correlation coefficient, stepwise multiple regression using the SPSS program. The results of this study were as follows 1. Mean score of self concept was 3.58, in minimum score 2.86 to maximum score 3.99. 2. Mean score of depression scale was 1.95, in a minimum score 1.50 to maximum score 2.61. 3. The relationship between self concept and depression was statistically significant negative correlation(r=-0.5121, p<0.001). 4. General characteristics variables significantly related to the level of self concept were marital status(t=-3.22, p<0.01), experience of disease in a year(t=2.76, p<0.01), child problem(t=-2.59, p<0.05), marital satisfaction(F=6.80, p<0.05). 5. General characteristics variables significantly related to the level of depression were possession of house(t=-2.42, p<0.01), experience of disease in a year(t=-4.32, p<0.001), child problem(t=3.12, p<0.05), husband problem(t=5.29, p<0.001), marital satisfaction (F=8.36, p<0.001). 6. Obstetrical characteristics variables significantly related to the level of self concept was times of pregnancy(F=3.54, p<0.05). 7. Obstetrical characteristics variables significantly related to the level of depression were age of menarche(F=3.87, p<0.05), times of abortion(F=2.49, p<0.01) number of children(F=3.36, p<0.01). 8. In the stepwise multiple regression analysis, the main influencing variables on depression of middle aged women were self concept($R^2$=.26, p<0.001), husband problem ($R^2$=.37, p<0.001), experience of disease in a year($R^2$=.41, p<0.001) and possession of house($R^2$=.44, p<0.001).

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