A quasi-experimental study was conducted to examine the effect of ice pack application to post cardiac surgery toddlers before C-tube removal. Twenty toddlers aged 13 months to 24 months, who were admitted to the hospital for open heart surgery during the period from July, 1993 to October, 1993 were randomly assigned to the experimental or control group. An ice pack was applied to the C-tube insertion site for 8 minutes before C-tube removal for the experimental group. The children were videotaped during the procedure to assess pain behavior and crying time. The score on the Modified Behavioral Pain Scale(MBPS), crying time, and vital signs were measured to determine the effect of ice pack application. The data were analyzed using Mann-Whitney U test, Paired t-test, and Pearson correlation coefficient. The result of the study are as follows : 1. There was a significant difference between the experimental group and the control group in pain behavior (U=17.0, p=.01). 2. There was no significant difference between the two groups in crying time. 3. There was a significant difference between the two groups in heart rate (U=24.5, p=.05). 4. There was a significant difference between the two groups in systolic blood pressure (U=24.0, p=.05) 5. There was a significant difference between the two groups in diastolic blood pressure (U=23.0, p=.04). 6. There was no significant difference between the two groups in respiration rate. 7. Pain behavior was significantly correlated with heart rate, systolic blood pressure, and crying time (r=.50, .54 and .59, p<.05). The result showed that the ice pack application was effective to reduce pain related to C-tube removal for the toddlers. From the study, it is recommended that the effectiveness of the cold ap-plication to children undergoing different painful procedures be examined to determine the most effective length of cold application to reduce pain in children.
It has been previously demonstrated that laccases exhibit great potential for use in several industrial and environmental applications. In this paper, two laccase isoenzyme genes, lccB and lccC, were cloned and expressed in Pichia pastoris GS115. The sequence analysis indicated that the lccB and lccC genes consisted of 1,563 and 1,584 bp, and their open reading frames encoded 520 and 527 amino acids, respectively. They had 72.7% degree of identity in nucleotides and 86.7% in amino acids. The expression levels of LccB and LccC were up to 32,479 and 34,231 U/l, respectively. The recombinant laccases were purified by ultrafiltration and $(NH_4)_2SO_4$ precipitation, showing a single band on SDS-PAGE, which had a molecular mass of 58 kDa. The optimal pH and temperature for LccB were 2.0 and $55^{\circ}C$ with 2,2'-azinobis-[ 3-ethylbenzthiazolinesulfonic acid (ABTS) as a substrate, whereas LccC exhibited optimal pH and temperature at 3.0 and $60^{\circ}C$. The apparent kinetic parameters of LccB were 0.43 mM for ABTS with a $V_{max}$ value of 51.28 U/mg, and the Km and $V_{max}$ values for LccC were 0.29 mM and 62.89 U/mg. The recombinant laccases were able to decolorize five types of dyes. Acid Violet 43 (100 g/ml) was completely decolorized by LccB or LccC (2 U/ml), and the decolorization of Reactive Blue KN-R (100 g/ml) was 91.6% by LccC (2 U/ml). Thus, the study characterizes useful laccase isoenzymes from T. versicolor that have the capability of being incorporated into the treatment of similar azo and anthraquinone dyes from dyeing industries.
Park, Kyoung-Ho;Shin, Hyon-Taek;Lee, Min-Hwa;Goh, Young-Yul
Journal of Pharmaceutical Investigation
/
v.22
no.3
/
pp.229-235
/
1992
The bioequivalence of two commercial choline magnesium trisalicylate (CMT) tablets was evaluated in 10 normal male subjects (age 21-27 yr, mean 23 yr) following single oral administrations of two products. Test product was $Trimax^{\circledR}$ tablet (Hyundai Pharm. Ind. Co., Ltd., Korea) and reference product was $Trilisate^{\circledR}$ tablet (Purdue Frederick, U.S.A.). Both products contained 500 mg salicylate. In the study, ten volunteers were administered one tablet of $Trimax^{\circledR}$ or $Trilisate^{\circledR}$ with randomized two period cross-over study. The pharmacokinetic parameters of two products were statistically compared using Student's t-test and ANOVA. When Student's t-test was applied, mean area under the curves (AUC) of $Trilisate^{\circledR}$ and $Trimax^{\circledR}$ were $388.88{\pm}74.99\; {\mu}g{\cdot}hr/ml$ and $390.63{\pm}63.02\;{\mu}g{\cdot}hr/ml$ hrlm!, respectively, which were not significantly different (p>0.05). The mean peak concentrations $(C_{max})$ and mean times to peak $(T_{max})$ of $Trilisate^{\circledR}$ and $Trimax^{\circledR}$ were $71.1{\pm}12.2$ and $72.9{\pm}10.7\;{\mu}g/ml$, and $72{\pm}33$ and $57{\pm}36min$, respectively, which were not significantly different (p>0.05). The mean terminal phase half-lives $(t_{l/2ter})$ of the two products were $2.57{\pm}0.47$ and $2.43{\pm}0.40$ hr, and also they were not significantly different (p>0.05). When ANOVA was applied, the parameters of the two products were not also significantly different each other. Based on the above results, it has been concluded that the bioavailability of $Trimax^{\circledR}$ tablet was not significantly different from that of $Trilisate^{\circledR}$ tablet.
Strain A-3, an amylase-producing bacteria, was isolated from coastal seawater near Daecheon in the Republic of Korea. It was seen to possess a single polar flagella and grow well, on ASW-YP agar plates, at temperatures of between $20-37^{\circ}C$. However, it grew more slowly at the temperatures of $15^{\circ}C$ and $40^{\circ}C$. Similarly, it was observed to grow abundantly, in an Artificial Sea Water-Yeast extract-Peptone (ASW-YP) liquid medium, in a pH range of 6-9, but not grow at pHs of 4-5 and a pH of 10. Strain A-3 was noted as being close to Pseudoalteromonas phenolica O-$BC30^T$, Pseudoalteromonas luteoviolacea $NCIMB1893^T$, Pseudoalteromonas rubra $ATCC29570^T$, and Pseudoalteromonas byunsanensis $FR1199^T$, with 98.30%, 97.86%, 97.78%, and 97.25% similarities respectively, in its 16S rRNA sequence. A phylogenetic tree revealed that strain A-3 and P. phenolica O-$BC30^T$ belong to a clade. However, strain A-3 differed from P. phenolica O-$BC30^T$ in relation to a number of physiological characteristics. Strain A-3 exhibited no growth above 5% NaCl concentrations, no utilization of D-glucose, D-mannose, D-maltose, or D-melibose, and no lipase (C-14) activity. All of these properties strongly indicate that strain A-3 is distant from P. phenolica O-$BC30^T$ and thus led us to name it Pseudoalteromonas sp. A-3. Pseudoalteromonas sp. A-3 produces ${\alpha}$-amylase throughout growth. Maximal amylase activities of 144.48 U/mL and 149.20 U/mL were seen at pH 7.0 and $37^{\circ}C$, respectively. Pseudoalteromonas sp. A-3's high, stable production of ${\alpha}$-amylase in addition to its biochemical features, such as alkalitolerance, suggest that it is a good candidate for industrial applications.
This study was attempted to identify the difference between body image of the patients with lower limb fracture and that of normal persons, and to examine if supportive group care could offer an apportunity for positive change in body image of the patient with lower limb fracture under the Quasi-experimental design. The subjects for this study were obtained by ta-king convenient sample of soldiers; the experimental group were 44 lower limb fractured patients hospitalized on the orthopedic Surgery unit in S Army General Hospital, while the control group were 44 normal enlisted men serving in the B Army regiment. Supportive group nursing care was given to the lower limb fractured patients who belong to the experimental group. Pre-and post-tests were administered to the experimental and the control group. The instruments to measure body image of the subjects were body Cathexis Scale developed by Scord and Jourard (1953) and Body Meaning Scale dove-loped by the reseacher. The reliability coefficients by Cronhach's u-test were .95 in body Cathexis Scale and .89 in Body Meaning Scale in this study. Data for this study were collected over a period 12 days from the 12th to the 24th of October, 1984 by the questionnaire. Data were analyzed by computer. Frequency, Percentage and x²-test were used to examine general chacteristics of the subjects. t-test was used to analyze the hypotheses. Analysis of variance was used to test difference in body image between groups classified by the general characteristics. Pearson Correlation Coefficient was used to identify the correlation between Body Cathexis Scale and Body Cathexis Scale and Body Meaning Scale. The results of this study were as follows: 1. No significant difference was found between the experimental and the control group on general characteristics of the subjects (p> .05). 2. Hypothesis I:“There will be a difference in body image between patiens with lower limb fracture and normal persons,”was supported(Body Cathexis t=6.91, p<.001, Body Meaning t=5.66, p< .001). 3. Hypothesis Ⅱ;“The will be a difference in body image of patients with lower limb fracture bet-ween after and before, supportive group nursing care was provided,”was supported (Body Cathexis t=5.90, p<.001, Body Meaning t=4.45, p <.001). 4. There was no significant difference in body image between groups classified by the general characteristics (p> .05). 5. The correlation between Body Cathexis Scale and Body Meaning Scale: It was reported that Body Cathexis Scale correlated with Body Meaning Scale in total subjects of the experimental and control group (r=.744, p<.001). That is, there was relatively high correlation between two scales. body Cathexis Scale correlated with Body Meaning Scale in the experimental group(r=.738, p <.001) and in the control group (r=.352, p <.001). That is, there was more than moderate correlation between two scales. In conclusion, it was found that there was a difference in body image between patients with lower limb fracture and normal persons, and supportive group nursing care offered an opportunity for positive change in body image of the patient with lower limb fracture.
Purpose: We investigated the association of effector memory (EM) CD8+ T cell and CD4+ T cell immunity with metabolic syndrome (MS). Methods: Surface and intracellular staining of peripheral blood mononuclear cells was performed. Anti-interleukin-7 receptor-alpha (IL-7Rα) and CX3CR1 antibodies were used to stain the subsets of EM CD8+ T cells, while anti-interferon-gamma (IFN-γ), interleukin-17 (IL-17), and forkhead box P3 (FOXP3) antibodies were used for CD4+ T cell subsets. Results: Of the 47 obese children, 11 were female. Children with MS had significantly higher levels of serum insulin (34.8±13.8 vs. 16.4±6.3 µU/mL, p<0.001) and homeostasis model assessment of insulin resistance (8.9±4.1 vs. 3.9±1.5, p<0.001) than children without MS. Children with MS revealed significantly higher frequencies of IL-7Rαlow CD8+ T cells (60.1±19.1% vs. 48.4±11.5%, p=0.047) and IL-7RαlowCX3CR1+ CD8+ T cells (53.8±20.1% vs. 41.5±11.9%, p=0.036) than children without MS. As the serum triglyceride levels increased, the frequency of IL-7RαlowCX3CR1+ and IL-7RαhighCX3CR1- CD8+ T cells increased and decreased, respectively (r=0.335, p=0.014 and r=-0.350, p=0.010, respectively), in 47 children. However, no CD4+ T cell subset parameters were significantly different between children with and without MS. Conclusion: In obese children with MS, the changes in immunity due to changes in EM CD8+ T cells might be related to the morbidity of obesity.
Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
/
v.7
/
pp.85-104
/
2003
In der vorliegenden Arbeit werden die deutschen Satzmodelle $f\"{u}r$ Zwecke des DaF-Unterrichts in Korea untersucht. Dabei gehen wir davon aus, dass man die deutschen Satzmodelle vergleichend mit den koreanischen neu aufbauen sollte, um sie im Deutschunterricht fur Koraner recht benutzen zu konnen. Dafur haben wir hier zunachst untersucht, wie man die koreanischen Satzmodelle im Hinblick auf den didaktischen Zweck modifizieren muss. Unter $Ber\"{u}cksichtigung$ des dabei erzielten Ergebnisses wurden $anschlie{\ss}end$end die deutschen Satzmodelle rekonstruiert. Im Koreanischen wurden die Satzmodelle ab Ende der 1960er Jahre verschiedenartig untersucht. Zwar gibt es bisher noch keine Arbeit, die Ergebnisse dieser Forschungen zusammenfasst, dennoch versuchen wir hier aus einigen bisherigen Untersuchungen einen begrenzten Rahmen von koreanischen Satzmodellen herauszufinden. Als Resultat haben wir funf grundlegende Satzmolle hergestellt: (1) Subjekt + $Pr\"{a}dikat$, (2) Subjekt + $Pr\"{a}dikativ$ + $Pr\"{a}dikat$, (3) Subjekt + Objektiv + $Pr\"{a}dikat$, (4) Subjekt + Adverbiale + $Pr\"{a}dikat$, (5) Subjekt + Obj. + Obj./$Pr\"{a}dikativ$/Advb. + $Pr\"{a}dikat$. Das Modell (1) $umfa{\ss}t$ drei $Pr\"{a}dikate$, die sich nach den Wortarten unterscheiden: (intransitives) Verb, Adjektiv und Substantiv. Auch beim Fall (5) $k\"{o}nnen$ drei verschiedene Untermodelle nach der $Kombinationsm\"{o}glichkeit$ auftreten: Sub + Obj. + $Pr\"{a}d$., Sub. + Obj. + $Pr\"{a}dikativ$ + $Pr\"{a}d$., Sub. + Obj. + Advb. + $Pr\"{a}d$. Auch im Deutschen kann man ahnlich wie im Koreanischen didaktisch geeignete Satzmodelle herausarbeiten. Auch dabei wurden bisherige Untersuchungen zu den deutschen Satzmodellen herangewgen. Zu der $d\"{a}fur$$ausgew\"{a}hlten$ Terminologie $geh\"{o}ren$ Subjekt(S), $Pr\"{a}dikat(P)$, $Pr\"{a}dkativerganzung(PE)$, $Objekterg\"{a}nzung(OE)$, $Adverbialerg\"{a}nzung(AE)$. In der deutschen Sprache werden nach den $Valenzm\"{o}glichkeiten$ des $Pr\"{a}dikativteils$ folgende Modelle unterschieden: S + P, S + P + PE, S + P + OE, S + P + AE, S + P + OE + OE/PE/AE. Unter die OE werden okk. OE, dat. OE, $pr\"{a}d$. OE, refl. OE usw. subsumiert. Um die Stichhaltigkeit der in dieser Schrift neu festgestellten Satzmodelle zu $\"{u}berpr\"{u}fen$, sollten sie anhand eines Textkorpus heutiger Texte in weiteren Untersuchungen validiert werden. Ferner ist es zu empfehlen, die Anwendbarkeit der hier vorgestellten Satzrnodelle im Deutschunterricht $f\"{u}r$ Koreaner $gr\"{u}ndlich$ zu testen
Journal of the Korean Society for Industrial and Applied Mathematics
/
v.9
no.1
/
pp.17-29
/
2005
In this paper we consider finite element methods for nonlinear hyperbolic integro-differential problems defined in ${\Omega}\;{\subset}\;R^d(d\;{\leq}\;4)$. A new initial approximation of $u_t(0)$ is taken. Optimal order error estimates in $L_p$ for $2\;{\leq}\;p\;{\leq}\;{\infty}$ are established for arbitrary order finite element. One order superconvergence in $W^{1,p}$ for $2\;{\leq}\;p\;{\leq}\;{\infty}$ are demonstrated as well.
Purpose: This study is a descriptive research study to determine the level of professional autonomy, professional self-concept, and job satisfaction of emergency nurses, and to investigate their correlations and verify the effects of professional autonomy and professional self-concept on job satisfaction. Method: The subjects were 189 emergency nurses with a work experience of 1 year or more, in 14 hospitals located in B and U Metropolitan Cities. The study was conducted from July 20, 2014, to August 30, 2014. The measurement instruments for professional autonomy, professional self-concept, and job satisfaction were used as the measurement tools. The collected data were analyzed using the t-test, analysis of variance, Scheffe's test, Pearson's correlation coefficient, and multiple linear regression. Results: Job satisfaction among emergency nurses showed a significant positive correlation with professional autonomy (r=.28, p <.001), and with professional selfconcept (r=.50, p <.001) with sub-areas of professional practice (r=.79, p <.001), satisfaction (r=.64, p <.001), and communication (r=.25, p <.001). Factors affecting job satisfaction were satisfaction (${\beta}$=0.60, p <.001), followed by low professional autonomy (${\beta}$=-0.24, p <.001) and communication (${\beta}$=0.14, p =.008), which accounted in total for 48.3% of the effect. Conclusion: This study suggests that enhancing professional satisfaction, maintaining proper communication, and securing autonomy are required to improve the job satisfaction among emergency nurses.
Fermented Chlorella vulgaris CBT$^{(R)}$ was evaluated for its effects on egg production, egg quality, liver lipids and intestinal microflora in laying hens. One hundred and eight Hy-line Brown layers (n = 108), 80 wk of age, were fed a basal diet supplemented with CBT$^{(R)}$ at the level of 0, 1,000 or 2,000 mg/kg, respectively for 42 d. Egg production was measured daily and egg quality was measured every two weeks. Five eggs from each replicate were collected randomly to determine egg quality. Egg production increased linearly with increasing levels of CBT$^{(R)}$ supplementation (p<0.05), although there was no significant effect of treatment on feed intake. Egg yolk color (p<0.001) and Haugh unit (p<0.01) improved linearly with increasing dietary CBT$^{(R)}$. Hepatic triacylglycerol level was linearly decreased with increasing dietary CBT$^{(R)}$ (p<0.05). The supplemental CBT$^{(R)}$ resulted in linear (p<0.001) and quadratic (p<0.01) response in population of cecal lactic acid bacteria. In conclusion, fermented Chlorella vulgaris supplemented to laying hen diets improved egg production, egg yolk color, Haugh unit and positively affected the contents of hepatic triacylglycerol and the profiles of cecal microflora.
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