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A $G_{4}$ Sequence within PHR1 Promoter Acts as a Gate for Cross-Talks between Damage-Signaling Pathway and Multi-Stress Response

  • Jang, Yeun-Kyu;Kim, Eun-Mi;Park, Sang-Dai
    • Animal cells and systems
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    • 제6권3호
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    • pp.271-275
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    • 2002
  • Rph1 and Gisl are damage-responsive repressors involved in PHR1 expression. They have two $C_{2}$H/ sub 2/ zinc finger motifs as putative DNA binding domains and N-terminal conserved domain with unknown function. They are also found in the human retinoblastoma binding protein 2 and the mouse jumonji- encoded protein. The repressors are able to bind to A $G_{4}$ sequence within a 39-bp sequence called upstream repressing sequence of PHR1 promoter (UR $S_{PHR1}$) responsible for the damage-response of PHR1. We report here that Rph1 is predominantly localized in the nucleus as examined by fluorescence microscopic analysis with GFP-Rph1 fusion protein. On the basis of the fact that the A $G_{4}$ sequence that is recognized by Rph1 and Gisl is also recognized by Msn2 and Msn4 in a process of stress response, we a1so tried to examine the in vivo function of A $G_{4}$ and the role of Msn2 and Msn4 in PHR1 expression. Our results demonstrate that Msn2 and Msn4 are actually required for the basal transcription of PHR1 expression but not for its damage induction. When A $G_{4}$ sequence was inserted into the minimal promoter of the cyc1-LacZ reporter, the increased LacZ expression was observed indicating its involvement in transcriptional activation. The data suggest that the A $G_{4}$ is primarily required for basal transcriptional activation of PHR1 or CYC1 promoter through the possible involvement of Msn2 and Msn4. However, since the A $G_{4}$ is also involved in the repression of PHR1 via Rphl and Gisl, it is proposed that A $G_{4}$ functions as either URS or upstream activating sequence (UAS) depending on the promoter context.t.

만성 폐쇄성 폐질환자의 자가간호와 자기효능감 및 사회적지지와의 관계 (Relationships among Self-care, Self-efficacy, and Social Support in Patients with Chronic Obstructive Pulmonary Disease)

  • 김란이;용진선
    • 한국간호교육학회지
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    • 제11권2호
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    • pp.270-277
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    • 2005
  • Purpose: This study was to investigate relationships among self-care, self-efficacy and social support in patients with chronic obstructive pulmonary disease (COPD). Method: The subjects were 114 patients with COPD from both inpatient and outpatient department sin a branch hospital of C university located in Gyeonggi-do from April 2005 to May 2005. Self-care was measured by No Hyeon-Suk's (2003) scale, self-efficacy was measured by Wigal et al's (1991) scale, social support was measured by Park Sun-Ju's (2001) scale. Data were analyzed by using t-test, ANOVA, Pearson correlation coefficient, and stepwise multiple regression. Results: The total mean was 3.1 for self-care, 3.5 for self-efficacy, and 4.3 for social support. The levels of self-efficacy and social support were significantly and positively correlated with the level of self-care. The level of self-care was influenced by social support explaining 16% and the level of self-efficacy explaining 6%, giving a total of 22%. Conclusion: The result of this study showed that the patients with COPD carried out self-care better when they had higher levels of self-efficacy and social support. Thus, we will improve the level of self-care of patients with COPD by nursing intervention to increase self-efficacy and social support.

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일차 냉각계통 스트레이너에 대한 내진 건전성 평가 (Seismic Evaluation for Strainer in the Primary Cooling System)

  • 정철섭
    • 한국전산구조공학회논문집
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    • 제13권3호
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    • pp.295-304
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    • 2000
  • 본 연구의 목적은 지진하중에 대한 응력 해석을 수행하여 ASME, Class 3 설계요건에 따라 스트레이너의 구조건진성을 평가하는 것이다. 스트레이너에 대한 설계요건이 ASME 코드 내에 명백하게 규정되어 있지 않기 때문에 본체는 밸브 설계요건인 ND-3500을 적용하고, 양쪽 플랜지 연결부는 배관 설계요건 중 ND-3658.3을 적용하였으며, 하단의 덮개 플랜지는 Appendix XI에 따라 설계 및 해석을 수행하였다. 본 연구에서는 T형 스트레이너를 쉘로 모델링하여 유한요소법을 사용하여 지진하중에 의해 스트레이너가 응답하는 모드 형상 및 고유진동수를 계산하여 충분히 강건한 구조물임을 입증한 후 정적 해석을 수행하여 주관과 분기 관이 접합하는 연결부위와 같은 위험단면에서의 막응력과 굽힘 응력을 구하였다. 각 하중조합에 대해 코드에서 규정하고있는 허용 값과 비교한 결과 스트레이너는 지진하중이 작용하는 경우 구조적 건전성을 유지하고 있음을 확인하였다. 아울러 인접 배관을 연결해주는 플랜지 연결부의 응력을 규정에 따라 구한 후 설계요건에 의한 허용 값과 비교하여 건전성을 만족함을 알 수 있었다.

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높은 LO-RF 격리 특성의 94 GHz MMIC Single-balanced Mixer (High LO-RF Isolation 94 GHz MMIC Single-balanced Mixer)

  • 안단;이복형;임병옥;김성찬;이상진;이문교;신동훈;박형무;박현창;김삼동;이진구
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2005년도 추계종합학술대회
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    • pp.765-768
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    • 2005
  • In this paper, high LO-RF isolation 94 GHz MMIC single-balanced mixer was designed and fabricated using a branch line coupler and a ${\lambda}/4$ transmission line. The 94 GHz MMIC single-balanced mixer was designed using the 0.1 ${\mu}m$ InGaAs/InAlAs/GaAs Metamorphic HEMT(MHEMT) diode. The fabricated MHEMT was obtained the cut-off frequency($f_T$) of 189 GHz and the maximum oscillation frequency($f_{max}$) of 334 GHz. The designed MMIC single-balanced mixer was fabricated using 0.1 ${\mu}m$ MHEMT MMIC process. From the measurement, the conversion loss of the single-balanced mixer was 23.1 dB at an LO power of 10 dBm. The LO-RF isolations of single-balanced mixer was obtained 45.5 dB at 94.19 GHz. We obtained in this study a higher LO-RF isolation compared to some other balanced mixers in millimeter-wave frequencies.

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지하철역사의 호선별로 미세먼지의 노출특성에 대한 평가 (Evaluation of Exposure Characteristics of Fine Dusts by Subway Lines)

  • 황성호;김종오
    • 한국환경보건학회지
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    • 제43권1호
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    • pp.71-76
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    • 2017
  • Objectives: This study aimed to assess the environmental factors that affect particulate matters (PM10) and to compare with outdoor PM10 concentrations in an underground subway stations. Methods: The PM10 level was determined from May 2013 to September 2013 in the Seoul subway stations in four lines. PM mini-vol portable sampler sampler was used to collect PM10 for 6 hrs. Arithmetic means of PM10 concentrations with standard deviation (SD) were calculated. Paired t-test was used to compare the differences between indoor PM10 and outdoor PM10 concentrations with correlation analysis which was used to identify the association between indoor PM10 concentrations and environmental factors. Results: There were no different PM10 concentrations significantly between line 1, 2, 3 and 4 in an underground subway stations. Passenger number was positively associated with PM10 concentration while construction year was negatively associated with PM10 concentrations. Indoor PM10 concentrations were significantly higher than those in outdoor PM10 concentrations. PM10 concentrations were higher in the stations which were constructed before 1990s rather than the stations constructed after 1990s. Conclusion: PM10 levels in the underground subway stations varied greatly depending on the construction year. Therefore, it might need to be more careful management to the stations which constructed in before 1990s.

부사관학과 학생들의 소방안전교육 경험과 생활안전의식에 관한 연구 (Research for the Experience of Fire Fighting Aafety Education and Living Safety Consciousness of Department of Noncommissioned Officer in College Students)

  • 이원주;권신영;강순형;나윤채
    • 한국안전학회지
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    • 제30권4호
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    • pp.159-167
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    • 2015
  • The main purpose of this study is to evaluate the awareness survey on the experience of fire fighting safety education and living safety consciousness of college students in the department of noncommissioned officer. Additionally, making provision of basic references and the effective fire fighting safety education is another important purpose of this study. In order to evaluate the awareness, the survey consist of 43 queries based on 6 queries for general characters, 9 queries for experiences of fire fighting safety education and 30 queries for living safety consciousness. The collected data from survey was analyzed using SPSS 20.0 win program. The interrelationship for general characters was confirmed using analysis of frequency, percentage, independent T-tset, ANONVA analysis, and regression analysis. The results show that the experience of fire fighting safety education and living safety consciousness of students from the combatant branch and ordnance corps are higher than those students from quartermaster corps. This may be attributed to the presence or absence of safety relevant courses on a curriculum. Therefore, the presence of safety courses on a curriculum should be one of important points for improving the experience of fire fighting safety education and living safety consciousness of these college students.

노인장기요양 인정조사원의 의사소통능력, 공감만족 및 공감피로가 소진에 미치는 영향 (The Effects of Communication Skills, Compassion Satisfaction, Compassion Fatigue on Burnout among Staff of Long-term Care Insurance for the Elderly in National Health Insurance Services in Korea)

  • 최형심;이경은;조은희
    • 한국직업건강간호학회지
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    • 제25권1호
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    • pp.19-28
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    • 2016
  • Purpose: This study was a descriptive research to identify the effects of communication skills, compassion satisfaction, compassion fatigue on burnout among staff of long term care insurance for the elderly in National Health Insurance Services in Korea. Methods: A descriptive cross-sectional design was used. The participants were 191 staffs of long-term care insurance in National Health Insurance Services. Data were collected via mail from the 24 branch offices which were randomly selected among the total of 226 centers of National Health Insurance Corporation. Stamm's professional quality of life (ProQOL) and Communication Skills Test tool were included in questionnaire to detect. SPSS/WIN 20.0 was used to conduct the descriptive statistics, t-test, ANOVA, correlation analysis, and multiple regression. Results: Compassion fatigue had a significant positive effect on burnout, while Compassion satisfaction had a negative effect on burnout. The explained variance for burnout was 69% and compassion fatigue was the most significant factor in burnout among staff of long term care insurance services. Conclusion: The results indicate that the factors influencing staffs' burnout are compassion fatigue, compassion satisfaction, and education level. Therefore, strategies to decrease compassion fatigue and improve compassion satisfaction are needed to decrease burnout for staffs of long-term care insurance in National Health Insurance Services.

고온 증기 파이프의 잔여수명 평가 (Remaining Life Assessment of High Temperature Steam Piping)

  • 윤기봉
    • Journal of Welding and Joining
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    • 제13권2호
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    • pp.12-24
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    • 1995
  • 최근에 국내 화력발전설비도 사용 기간이 30년을 넘게 됨에 따라, 고온설비의 경년열화도 평가 및 수명예측 기술에 대한 연구가 활발해지고 있다. 본 논문에서는 l965년부터 사용되어 노 후된 영월화력발전소 2호기의 주증기 파이프를 대상으로 실시한 열화도 및 수명평가 결과를 보고하였다. 주증기관의 취약부인 맞대기 용접부, 지류 용접부, Y부 및 T부 등의 용접부에 대해 표면복제법, 경도측정법을 사용하여 수명평가를 실시하였으며, 비파괴 검사에 의해 균열이 탐지된 경우에는 수명평가 컴퓨터 코드를 사용하여 균열성장에 의한 잔여수명을 계산하였다. 또한 파이프 모재의 잔여 수명은 해석적 방법에 의해 정량적으로 잔여수명을 계산한 후, 외경 패출량 측정, 입계부식법 등에 의해 재질 열화도를 정성적으로 평가하였다. 본 논문에서 사용한 기법 이외의 방법을 사용하여 수명평가 정확도를 개선하는 방향에 대한 의견도 제시하였다.

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Characterization of macroalgal epiphytes on Thalassia testudinum and Syringodium filiforme seagrass in Tampa Bay, Florida

  • Won, Boo-Yeon;Yates, Kim K.;Fredericq, Suzanne;Cho, Tae-Oh
    • ALGAE
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    • 제25권3호
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    • pp.141-153
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    • 2010
  • Seagrass epiphyte blooms potentially have important economic and ecological consequences in Tampa Bay, one of the Gulf of Mexico's largest estuaries. As part of a Tampa Bay pilot study to monitor the impact of environmental stresses, precise characterization of epiphyte diversity is required for efficient management of affected resources. Thus, epiphyte diversity may be used as a rational basis for assessment of ecosystem health. In May 2001, epiphytic species encompassing green, brown and red macroalgae were manually collected from dense and sparse seagrass beds of Thalassia testudinum and Syringodium filiforme. A total of 20 macroalgal epiphytes, 2 Chlorophyta, 2 Phaeophyta, and 16 Rhodophyta, were found on T. testudinum and S. filiforme seagrass at the four sampling sites (Bishop Harbor, Cockroach Bay, Feather Sound, and Mariposa Key). The Rhodophyta, represented by 16 species, dominated the numbers of species. Among them, the thin-crusted Hydrolithon farinosum was the most commonly found epiphyte on seagrass leaves. Species number, as well as species frequency of epiphytes, is higher at dense seagrass sites than sparse seagrass sites. Four attachment patterns of epiphytes can be classified according to cortex and rhizoid development: 1) creeping, 2) erect, 3) creeping & erect, and 4) erect & holding. The creeping type is characterized by an encrusting thallus without a rhizoid or holdfast base. Characteristics of the erect type include a filamentous thallus with or without a cortex, and a rhizoid or holdfast base. The creeping and erect type is characterized by a filamentous thallus with a cortex and rhizoid. A filamentous thallus with a cortex, holdfast base, and host holding branch is characteristics of the erect and holdfast attachment type. This study characterized each species found on the seagrass for epiphyte identification.

황기의 녹비작물 작부체계 활용 유기재배 시 생장반응과 수량에 관한 연구 (Effects of Organic Fertilizers and Green Manure Crops on Growth Responses and Yields of Astragalus membranaceus Bunge)

  • 송범헌;이경아;장윤기;김영국;안태진;안영섭;박충범
    • 한국약용작물학회지
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    • 제19권2호
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    • pp.83-89
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    • 2011
  • This studies were conducted to investigate the growth responses and yields of Astragalus membranaceus Bunge which were grown with organic cultivation using two organic fertilizers and two green manure crops of hairy vetch and rye in 2008 and 2009. The lengths of stems and roots were not clearly different between organic and conventional cultivations. The root diameters and the branch numbers were slightly thicker and more in all treatments of organic cultivation than that of conventional cultivation in 2008, although they were not significantly different between two cultivating methods in 2009. The dry weights of shoot and root were heavier on organic cultivation than those on conventional cultivation. The T/R ratios were commonly higher in organic culture, showing more clearly in 2008 than 2009. The yields were generally higher in organic cultivation than those in conventional cultivation in 2008 although the effect did not clear on yield in 2009. The organic cultivation using of organic fertilizers and green manure crops would be new method to have safety and qualitative products. The techniques of organic cultivation for Astragalus membranaceus Bunge would be studied more on utilizing the natural organic resources.