• Environmental Engineering Research
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• v.24 no.4
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• pp.559-565
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• 2019

In order to alleviate the environmental pressure caused by production process of thermal power plants, the application of cleaner production is imperative. To estimate the implementation effects of cleaner production in thermal plants and optimize the strategy duly, it is of great significance to take a comprehensive evaluation for sustainable development. In this paper, a hybrid model that integrated the analytic hierarchy process (AHP) with least squares support vector machine (LSSVM) algorithm optimized by grid search (GS) algorithm is proposed. Based on the establishment of the evaluation index system, AHP is employed to pre-process the data and GS is introduced to optimize the parameters in LSSVM, which can avoid the randomness and inaccuracy of parameters' setting. The results demonstrate that the combined model is able to be employed in the comprehensive evaluation of the cleaner production in the thermal power plants.

• Proceedings of the KIEE Conference
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• 2003.07b
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• pp.1132-1134
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• 2003

This paper presents the realization of the vector control for AC servo motor using a high performance DSP, TMX320F2812. This DSP has some special peripheral circuits to drive a AC Servo motor as AD converter, QEP and so on. It makes us reduce the time of developing a control system and also can be simple size controller. The vector control algorithm for instantaneous torque control and SVPWM algorithm by offset voltage methods is adopted and we got the satisfactory results.

• The Transactions of the Korean Institute of Power Electronics
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• v.22 no.3
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• pp.263-269
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• 2017

This paper proposes a novel circulating current control method for an MMC-HVDC system based on Vector PI control. The method can suppress second-order harmonics of the circulating currents under balanced and unbalanced grid conditions. The proposed method is robust to grid frequency variation. The effectiveness of the proposed method is verified through frequency response and time domain simulation.

• Proceedings of the KIEE Conference
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• 1993.07b
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• pp.711-714
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• 1993

A predictive current controller for the Battery Energy Stroage System(BESS) based on space vector PWM of transformer coupled inverters is presented. The control method have many advantages such as accurate control, reduced harmonics, good dynamics, improved stability, wide control range, etc,. The simulation results show that the predictive control method with space vector PWM is suitable for the transformer coupled inverters applied to the battery energy storage system.

• Proceedings of the KIPE Conference
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• 2016.11a
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• pp.7-8
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• 2016

본 논문에서는 모듈형 멀티레벨 컨버터 (Modular Multilevel Converter ; MMC) 고압 직류 송전 (High Voltage Direct Current ; HVDC) 시스템의 순환전류 성분을 제어하기 위해 VPI (Vector Proportional Integral) 제어기를 적용한 순환전류 제어 기법을 제안하였다. 제안한 방식은 기존의 준공진 (Quasi-Resonant) 제어기를 적용한 방식과 비교하여 계통 주파수 변동에 강인하고 넓은 공진 대역폭 선정이 용이한 장점을 갖는다. 제안한 순환전류 제어기의 성능은 기존의 준공진 제어기를 적용한 기법과 계통 전압 단상지락 및 계통 주파수 변동 상황에서 시뮬레이션을 통하여 비교 검증하였다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.4
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• pp.2038-2057
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• 2017

Gaussian learning is a new technology in the computational intelligence area. However, this technology weakens the learning ability of a particle swarm and achieves a lack of diversity. Thus, this paper proposes a vector Gaussian learning strategy and presents an effective approach, named particle swarm optimization based on vector Gaussian learning. The experiments show that the algorithm is more close to the optimal solution and the better search efficiency after we use vector Gaussian learning strategy. The strategy adopts vector Gaussian learning to generate the Gaussian solution of a swarm's optimal location, increases the learning ability of the swarm's optimal location, and maintains the diversity of the swarm. The method divides the states into normal and premature states by analyzing the state threshold of the swarm. If the swarm is in the premature category, the algorithm adopts an inertia weight strategy that decreases linearly in addition to vector Gaussian learning; otherwise, it uses a fixed inertia weight strategy. Experiments are conducted on eight well-known benchmark functions to verify the performance of the new approach. The results demonstrate promising performance of the new method in terms of convergence velocity and precision, with an improved ability to escape from a local optimum.

• The Plant Pathology Journal
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• v.28 no.1
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• pp.81-86
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• 2012

We developed a reassortant RNA virus vector derived from $Cucumber$ $mosaic$ $virus$ (CMV), which has advantages of very wide host range and can efficiently induce gene silencing in a few model plants. Certain CMV isolates, however, show limited host ranges presumably because they naturally co-evolved with their own hosts. We used a reassortant comprised of two strains of CMV, Y-CMV and Gn-CMV, to broaden the host range and to develop a virus vector for virus-induced gene silencing (VIGS). Gn-CMV could infect chili pepper and tomato more efficiently than Y-CMV. Gn-CMV RNA1, 3 and Y-CMV RNA2-A1 vector were newly reconstructed, and the transcript mixture of RNA1 and 3 genomes of Gn-CMV and RNA2 genome of Y-CMV RNA2 containing portions of the endogenous phytoene desaturase (PDS) gene (CMV2A1::PDSs) was inoculated onto chili pepper (cv. Chung-yang), tomato (cvs. Bloody butcher, Tigerella, Silvery fir tree, and Czech bush) and $Nicotiana$ $benthamiana$. All the tested plants infected by the reassortant CMV vector showed typical photo-bleaching phenotypes and reduced expression levels of $PDS$ mRNA. These results suggest that the reassortant CMV vector would be a useful tool for the rapid induction of the RNA silencing of endogenous genes in chili pepper and tomato plants.

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.257-262
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• 2010

The purpose of this study is to establish a basic culture system enabling in vitro culture of chicken blastodermal cells and to test the feasibility of retrovirus-mediated gene transfer to the cultured cells. The blastodermal cells were isolated from freshly laid eggs of stage X and cultured with or without STO feeder layer cells. Stem cell-like morphology was maintained after multiple passages and RT-PCR analysis proved expression of several stem cell specific genes. Immunocytochemical analysis using antibodies of anti-EMA-1 and anti-SSEA-1 also showed the feature of stem cells. Infection of the cultured blastodermal cells with LNCGW retrovirus vector resulted in successful transfer of foreign genes. The results of this study may be useful in establishing stem cell-mediated transgenic chicken production.

• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.251-256
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• 2011

Human growth hormone (hGH), one of the most important hormones in medicine, is secreted from anterior pituitary gland. Its broad physiological function includes body growth, cell regeneration, increasement of muscle volume, bone density, body fat reduction, and so on. Due to the wide range of therapeutic effects, the hGH produced from E. coli has been commercialized already. In this study, we asked whether it is possible to produce recombinant hGH efficiently from various cultured mammalia cells. To meet this purpose, we chose a retrovirus vector system for transfer and expression of the hGH gene in various mammalian cells. Analyses of RT-PCR, ELISA, and Western blot to determine expression of the hGH gene showed the highest production of the hGH was determined from chicken embronic fibroblast (CEF) cells with the concentration of 8.58 ${\mu}g$/ml. The biological activity of the hGH was similar to the commercially available counterpart. These results suggest that mass production of hGH is possible not only in the E. coli but also in the various mammalian cells.

• Reproductive and Developmental Biology
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• v.40 no.1
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• pp.7-13
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• 2016

Effectiveness of transgene transfer into genome is crucially concerned in mass production of the bio-pharmaceuticals using genetically modified transgenic animals as a bioreactor. Recently, the mammary gland has been considered as a potential bioreactor for the mass production of the bio-pharmaceuticals, which appears to be capable of appropriate post-translational modifications of recombinant proteins. The mammary gland tissue specific vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals. In this study, to minimize physiological disturbance caused by constitutive over-expression of the exogenous gene, we constructed new retrovirus vector system designed for mammary gland-specific expression of the hEPO gene. Using piggyBac vector system, we designed to express hEPO gene under the control of mammary gland tissue specific and lactogenic hormonal inducible goat ${\beta}$-casein or mouse Whey Acidic Protein (mWAP) promoter. Inducible expression of the hEPO gene was confirmed using RT-PCR and ELISA in the mouse mammary gland cells treated with lactogenic hormone. We expect the vector system may optimize production efficiency of transgenic animal and reduce the risk of global expression of transgene.