Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Expression of Human Growth Hormone Gene using Retrovirus Vector System In Vitro

In Vitro에서 Retrovirus Vector System을 이용한 인간 성장 호르몬 유전자의 발현

  • Kim, Min-Ki (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Koo, Bon-Chul (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Kwon, Mo-Sun (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Kim, Te-Oan (Department of Physiology, Catholic University of Daegu School of Medicine)
  • 김민기 (대구가톨릭대학교 의과대학 생리학교실) ;
  • 구본철 (대구가톨릭대학교 의과대학 생리학교실) ;
  • 권모선 (대구가톨릭대학교 의과대학 생리학교실) ;
  • 김태완 (대구가톨릭대학교 의과대학 생리학교실)
  • Received : 2011.09.02
  • Accepted : 2011.09.09
  • Published : 2011.09.30
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Abstract

Human growth hormone (hGH), one of the most important hormones in medicine, is secreted from anterior pituitary gland. Its broad physiological function includes body growth, cell regeneration, increasement of muscle volume, bone density, body fat reduction, and so on. Due to the wide range of therapeutic effects, the hGH produced from E. coli has been commercialized already. In this study, we asked whether it is possible to produce recombinant hGH efficiently from various cultured mammalia cells. To meet this purpose, we chose a retrovirus vector system for transfer and expression of the hGH gene in various mammalian cells. Analyses of RT-PCR, ELISA, and Western blot to determine expression of the hGH gene showed the highest production of the hGH was determined from chicken embronic fibroblast (CEF) cells with the concentration of 8.58 ${\mu}g$/ml. The biological activity of the hGH was similar to the commercially available counterpart. These results suggest that mass production of hGH is possible not only in the E. coli but also in the various mammalian cells.

Keywords

References

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