Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Retrovirus Vector-Mediated Gene Transfer to the Chicken Blastodermal Cells Cultured In Vitro

체외 배양된 닭 배반엽 세포에 대한 Retrovirus Vector를 이용한 유전자 전이

  • 박성준 (대구가툴릭대학교 의과대학) ;
  • 구본철 (대구가툴릭대학교 의과대학) ;
  • 권모선 (대구가툴릭대학교 의과대학) ;
  • 최휘건 (대구가툴릭대학교 의과대학) ;
  • 김태완 (대구가툴릭대학교 의과대학)
  • Received : 2010.09.15
  • Accepted : 2010.09.20
  • Published : 2010.09.30
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Abstract

The purpose of this study is to establish a basic culture system enabling in vitro culture of chicken blastodermal cells and to test the feasibility of retrovirus-mediated gene transfer to the cultured cells. The blastodermal cells were isolated from freshly laid eggs of stage X and cultured with or without STO feeder layer cells. Stem cell-like morphology was maintained after multiple passages and RT-PCR analysis proved expression of several stem cell specific genes. Immunocytochemical analysis using antibodies of anti-EMA-1 and anti-SSEA-1 also showed the feature of stem cells. Infection of the cultured blastodermal cells with LNCGW retrovirus vector resulted in successful transfer of foreign genes. The results of this study may be useful in establishing stem cell-mediated transgenic chicken production.

Keywords

References

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