• Food Science and Preservation
• /
• v.15 no.5
• /
• pp.731-735
• /
• 2008

The coliform group, Salmonella typhimurium and Staphylococcus were analyzed for eliminating of microorganism spore which could be embeded in honey and eradication of studied for heat resistance of thermoacidophilic bacteria was studied for marketing of honey after producing honey drink. The method for analyzing of heat resistance thermoacidophilic bacteria was membrane-seperated cell culture with $0.45\;{\mu}m$ micro-filter and vacuum aspirator. The results of bacteria, coliform group, Salmonella typhimurium and Staphylococcus was negative, but normal method such as sterilization with electrolyzed water, normal micro-filter, high-temperature heating and microwave did not have effect on heat resistance thermoacidophilic bacteria. Also, absolute type micro-filter of $0.45\;{\mu}m$ and $0.8\;{\mu}m$ microfilteration showed higher effect on heat resistance thermoacidophilic bacteria than micro-filter of normal type, showing negative results at all treatments.

• Food Science of Animal Resources
• /
• v.42 no.4
• /
• pp.557-565
• /
• 2022

The purpose of this research was to examine the effectiveness of pre-cooking treatments on the quality characteristics of ready-to-eat (RTE) empal gentong. Raw beef meat was pre-cooked in water bath at 90℃ for 0 min (C), 10 min (T1), 20 min (T2), and 30 min (T3) prior to retorting process at 121℃ and pressure at 70,000 Pa. Results showed that pre-cooking treatments in all treated samples could reduce fat contents in empal gentong's meat by 0.02% (T1), 0.28% (T2), and 1.13% (T3) respectively. Highest precooking time tends to increase the pH and CIE a^* values. However, CIE b^* values, water holding capacity, and sensory analysis were not affected by pre-cooking duration which must have been affected by sterilization process after pre-cooking. In conclusion, pre-cooking treatment before sterilization in producing empal gentong is a probable technique to reduce its fat content and improve its physical quality. A specific treatment at 90℃ for 10 min is recommended to achieve optimum quality of RTE empal gentong's meat.

• Food Science and Biotechnology
• /
• v.15 no.4
• /
• pp.499-505
• /
• 2006

Clostridium botulinum spores are widely distributed in nature. Type A and proteolytic type B bacteria produce heat-resistant spores that are primarily involved in most of the food-borne botulism outbreaks associated with low-acid canned foods. Food-borne botulism results from the consumption of food in which C. botulinum has grown and produced neurotoxin. Growth and toxin production of type A and proteolytic type B in canned foods can be prevented by the use of thermal sterilization alone or in combination with salt and nitrite. The hazardousness of C. botulinum in low-acid canned foods can also be reduced by preventing post-process contamination and introducing hazard analysis and critical control point (HACCP) practices during production. Effectiveness of non-thermal technologies such as high pressure processing with elevated process temperatures on inactivation of spores of C. botulinum will be discussed.

• Food Science and Preservation
• /
• v.12 no.4
• /
• pp.317-322
• /
• 2005

The purposes of this study were to assess the microbiological contamination level of various disposable kitchen utensils and evaluate the effectiveness of gamma irradiation as a sterilization process for the utensils. The 51 kinds in 17 groups of disposable kitchen utensils were tested for the enumeration of total aerobic plate count, coliforms, fungi and Salmonella. Generally, cell density of microorganisms in disposable kitchen utensils were lower than that of cooking utensils currently using in kitchen. The survivals of total aerobic plate counts, putrefactive bacteria, on the surface of the disposable utensils were ranged up to $10^3\;CFU/100\;cm^2$. Filamentous fungi were detected in 13 samples. Coliform bacteria were detected in two kinds of samples but Salmonella spp. was detected below detection limits in all the samples. The microorganisms survived on the surface of utensils were effectively eliminated by gamma irradiation process at 3 kGy.

• Restorative Dentistry and Endodontics
• /
• v.1 no.1
• /
• pp.43-49
• /
• 1975

This study was divided into two parts. In the first experiment, the in vitro antimicrobial effect was tested in order to evaluate the effect of vapors, and the effectiveness of the nonspecific endodontic medicaments (formocresol, camphorated parachlorophenol and eugenol). In the second experiment, the intracanal effect was tested in vitro under simulated clinical condition. The actual bactericidal effect of the nonspecific endodontic medicaments (formocresol, camphorated parachlorophenol and eugenol) was quantitated. The results were obtained as follows: 1. The zone of inhibition was appeared on the vapors of formocresol only, however there were no zone of inhibition appeared on the vapors of camphorated parachlorophenol and eugenol. 2. Formocresol produced the widest zone of inhibition and eugenol, the next and camphorated parachlorophenol, the narrowest. 3. All of the tested medicaments were vaporized in the root canal. They proved to be the effective antimicrobial activity in the root canal. 4. All of the tested medicaments were showed more bactericidal effect at 72 hours than 48 hours. 5. In comparing with the bactericidal effect of the tested medicaments in the root canal, formocresol was showed the most bactericidal medicament, camphorated parachlorophenol was showed the least. 6. Complete sterilization of the root canal was not achieved in any medicaments applied in this study.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.17 no.6
• /
• pp.581-586
• /
• 2005

Recently, the use of UVGI system has been increasing in both medical and nonmedical buildings for the control of environmental microorganisms. In the present study, irradiance performance test of UVC lamp was carried out and indoor air sterilization effect of UV ray for preventing transimission of air borne contagion was investigated by using manufactured UVC air sterilizer. Experimental results show that the effective irradiance of UVC lamp is strongly dependent on air velocity and temperature in irradiance performance test. An individual microbiological killing effectiveness experiment also shows that the average kill rate of two microbiological samples such as bacteria and fungus is about $92\%$ by using manufactured UVC air sterilizer. Additionally irradiance performance experimental results also show that the ballast is very important factor to keep up irradiance performance of UVC lamp.

• Food Science and Preservation
• /
• v.13 no.2
• /
• pp.246-251
• /
• 2006

Organic compost is a useful fertilizer for organic farming. However, it poses a microbiological hazard to the farm produce because most of the compose are originated from excrementitious matters of domestic animals. Irradiation was performed to improve microbiological safety of organic compost and the effectiveness of gamma-irradiation for inactivating Salmonella typhimurium and E. coli was investigated. Total aerobic bacteria and coliform bacteria in 16 produce or commercial compose were ranged from $10^5\;to\;10^7\;CFU/mL\;and\;0\;to\;10^3\;CFU/mL$, respectively. All coliform bacteria in the composts were eliminated by irradiation at 5 kGy, while about $10^2\;CFU/mL$ of total aerobic bacteria survived up to 10 kGy of irradiation. In the inoculation test the test organisms (inoculated at $10^7\;CFU/mL$) were eliminated by irradiation at 3 kGy. $D_{10}$ values of inoculated Salmonella typhimurium and E. coli in the compost were $0.4{\pm}0.05\;and\;0.39{\pm}0.03kGy$. It was considered that $3{\sim}5kGy$ of gamma irradiation was effective for radicidation (radiation sterilization of pathogenic microbes) of organic fertilizer.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.40 no.4
• /
• pp.268-273
• /
• 2013

This study aims to investigate contamination level and effective clinical disinfection methods of dental air/water syringes (AWS) by using microbial incubation. This study used AWS of ten dental unit chairs of Hospital. Total 180 samples were obtained. There are six groups of samples: non-sterilized (group 1), sanitized with wet-gauze (group 2), sanitized with 78% ethanol sponge for 10 seconds (group 3), sanitized with 78% ethanol sponge for 20 seconds (group 4), sanitized with 1 : 100 diluted High Level Disinfectant$^{(R)}$ (group 5), autoclaved (group 6). Group 1 and 2 showed statistically significant level of CFUs than Group 4, 5 and 6 (p < 0.05). Group 4, 5 and 6 did not show any noticeable CFU. Sanitizing AWS tips with ethanol and High level Disinfectant$^{(R)}$ proves to be a useful and practical method for preventing cross-infections.

• Journal of the Korean Wood Science and Technology
• /
• v.35 no.6
• /
• pp.50-56
• /
• 2007

FAO standard for heat sterilization of wood, International standards for phytosanitary measures (ISPM) No.15, must meet heat-treated wood core temperature to be higher than $56^{\circ}C$ and keep the temperature for more than 30 minutes. This study was carried out to analyze the heat treatment characteristics of domestic pinewood sterilized with the FAO standard. To enhance the effectiveness of heat treatment process in mountainous district energy consumption and time required to reach target temperature were evaluated at various temperature and relative humidity conditions and moisture contents of wood. Heat-treatment of high temperature and high humidity reduced the required heating time. Lower humidity levels at same temperature reduced energy consumption per unit time. However, lower humidity levels could not reduce total energy consumption greatly because longer treatment time was required at that condition. It is necessary to estimate energy consumption and predict treatment time in dynamic heating and cooling situations, because it frequently happens not to meet optimum treatment condition due to poor surrounding climates and operation performance of heat treatment facility in real field.

• Journal of Marine Life Science
• /
• v.4 no.2
• /
• pp.86-90
• /
• 2019

This study aimed to compare the disinfection efficiencies of the ultraviolet and plasma systems, the two systems designed and commercialized to disinfect water in aquaculture, by putting each in a 100 ℓ water tank and concentrating 1.0 ℓ of treated water to check the changes in the number of bacteria in the samples. Each system was operated for 6 hours to culture the typical seawater bacteria in the Marine agar, Thiosulfate citrate bile salts sucrose agar and Salmonella Shigella agar media, respectively, to check the number of bacteria in the media, and the changes in the number of Edwardsiella piscicida in the treated water were checked after the artificial inoculation of E. piscicida in the disinfected seawater. As a result, the two disinfection systems showed the almost similar levels of bacterial reduction efficiency between 99.5% and 99.9%. However, the result of this study showed that, with 100 ℓ of water treated for the same length of time using the two systems, the plasma system turned out to disinfect bacteria in a shorter period of time than the UV system. However, as the changes in the number of bacteria were checked for a short length of time (6 hours) in this study, it was judged that, considering the actual aquaculture environment in which the quality of water significantly changes with feed residues, excretions and coastal contamination, etc., and a lot of biofilms and organic matter exist, the plasma system would be more efficient than the UV system as the former is capable of continuously maintaining a certain level of efficiency than the latter that is limited in terms of efficiency depending on the level of turbidity and the existence of organic matter.