Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Sterilization Effectiveness by Nominal and Absolute Filter in Pre-treatments of Honey

벌꿀의 전처리 공정 중 Nominal 및 Absolute Filter 적용을 통한 제균 효과에 관한 연구

  • Published : 2008.10.31
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Abstract

The coliform group, Salmonella typhimurium and Staphylococcus were analyzed for eliminating of microorganism spore which could be embeded in honey and eradication of studied for heat resistance of thermoacidophilic bacteria was studied for marketing of honey after producing honey drink. The method for analyzing of heat resistance thermoacidophilic bacteria was membrane-seperated cell culture with $0.45\;{\mu}m$ micro-filter and vacuum aspirator. The results of bacteria, coliform group, Salmonella typhimurium and Staphylococcus was negative, but normal method such as sterilization with electrolyzed water, normal micro-filter, high-temperature heating and microwave did not have effect on heat resistance thermoacidophilic bacteria. Also, absolute type micro-filter of $0.45\;{\mu}m$ and $0.8\;{\mu}m$ microfilteration showed higher effect on heat resistance thermoacidophilic bacteria than micro-filter of normal type, showing negative results at all treatments.

본 연구는 벌꿀의 내포되어 있을 수 있는 미생물의 포자의 제균에 관한 연구로 선행연구로 일반세균, 대장균군, 살모넬라균, 포도상구균을 분석하였으며, 벌꿀 음료 제조 후 유통 중에 문제시 되어지고 있는 내열호산성균의 제균에 관한 연구결과이다. 분석을 위한 내열호산성균 실험법은 진공감압기를 이용하여 $0.45\;{\mu}m$ micro filter를 이용한 막분리 배양법을 이용하였다. 미생물 실험 결과 일반세균, 대장균군, 살모넬라균, 포도상구균은 모두 음성으로 나타났으며, 내열호산성균 제균을 위하여 식품의 일반 살균법을 적용한 결과 농도별 전해수 살균, 오존수 살균, 자외선살균, 일반 마이크로 필터 처리, 고온 가열 살균, microwave 살균 등 모든 제균 방법에서 균이 제어 되지 않는 것으로나타났다. 또한 마이크로 필터를 이용한 제균 처리에서도 일반 (nominal type) 필터를 사용하였을 경우 $44.8{\sim}64.5%$ 제균 효과를 나타낸 반면 absolute type의 필터를 사용하였을 경우 0.45, $0.8\;{\mu}m$ 마이크로 필터의 사용 시 모든 처리구에서 음성으로 나타나 제균 효과가 다른 살균법에 비해우수한 것으로 나타났다.

Keywords

References

  1. Choi, Y.J (1997). Beekeeping.bees and a wooden beehive. Ohsungbooks
  2. Keum, J.I. (1982) The newest beekeeping. o-sung, 263
  3. White, J.W. (1978) Honey. Advances in food research, 24, 288
  4. Kim, B.N., Kim, T.J. and Choi, H.S. (1994) Study of mineral, HMF and vitamin contents of kangwon-do honeys. Kor. J. Food Nutr. Technol., 23, 675-
  5. Kim, J.G., Kim, M.K. and Lee, S.H. (1993) Study on the contents of oligosaccharides in honey by honey plants. Kor. J. Apiculture, 8, 165-169
  6. Korea Foods Industry Association. (2000) Food Code. 580-585
  7. Lee, Y.W. and Park, S.G. (1996) Food hygiene microorganism test. 98-139
  8. Korea Foods Industry Association. (2006) Food Code (separate volume). 98
  9. A.O.A.C. (1990) Association of offcial analytical chemists. 15th. ed.
  10. Fabian, F.W. (1935) The use of honey in making fermented drinks. The Fruit prod. J., 14, 363-365
  11. Chung, D.H. and Baek, S.H. (2002) Antibacterial activities of honeys on the Staphylococcus aureus. Korean J. Food Nutr., 2, 158-164
  12. Irish, J., Carter,D.A., Blair, S.E. and Heard T.A (2008) Antibacterial activity of honey against community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) Int. J. Antimicrob. Agents, 32, 89-90 https://doi.org/10.1016/j.ijantimicag.2008.02.012
  13. Basualdo, C., Sgroy, V., Finola, M.S. Marioli,J. M. (2007) Comparison of the antibacterial activity of honey from different provenance against bacteria usually isolated from skin wounds. Vet. Microbiol., 124, 375-381 https://doi.org/10.1016/j.vetmic.2007.04.039
  14. Weston, R. J., Mitchell, K. R., Allen, K. L.(1999) Antibacterial phenolic components of New Zealand manuka honey. Food Chemistry, 64, 295-301 https://doi.org/10.1016/S0308-8146(98)00100-9
  15. Jung, S.W. Jeong, W.J. and Park, K.J. (1999) Microbial removal effects of electrolyzed acid water on lettuce by washing methods and quality changes during storage. Korean J. Food Sci. Technol., 6, 1511-1517
  16. Park, U Po (2004) Quality Characteristics of Salted Chinese Cabbage Treated with Electrolyzed-Acid Water during Storage. Korean J. Food Sci. Technol.,. 2, 365-367
  17. Jung, S.W. Jeong, W.J. Lee, S.H. Park N.H. (1999) Changes in Quality of Crown Daisy and Kale Washed with Cooled Electrolyzed Acid Water during Storage. Korean J. Food Preserv., 4, 417-423
  18. Kim, S.S., Lee, J.H. and KIm, S.Y.(1999) Pasteurization efficientcy of continuous microwave HTST system for milk. Korean J. Food Sci. Technol., 5, 1392-1396
  19. Kim, S.S. and Lee, J.H. (1999) Pasterization efficiency and physico-chemical changes of soymilk HTST pasterized using mictowave. Korean J. Food Sci. Technol., 31, 1196-1202
  20. Keum, J.S. and Cho, Y.S. (2000) Sterilization Method using microwave in food industry. Food Sci. Ind., 33(2), 12-18
  21. Karabournioti, S. and Zervalaki, P. (1990) The effect of heating on honey HMF and invertase. Apiacta, 36, 177-181
  22. Tosi, E.A., Re. E., Lcero, H. and Bulacio, L. (2004) effect of honey high-temperature short-time heating on parameters related to quality crystallization phenomena and fungal inhibition. Lebensm. Wiss. Technol., 37, 669-678 https://doi.org/10.1016/j.lwt.2004.02.005