Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Effect of Acetic Acid Fermented Juice Prepared Using Submerged Culture Media of Antrodia camphorata Mycelium on Blood Glucose and Lipid Profiles of Rats in which Diabetes was Induced with Streptozotocin

우장지 버섯(Antrodia camphorata) 균사체 배양액으로 제조한 초산발효액이 Streptozotocin으로 유발한 당뇨흰쥐의 혈당과 혈중지질함량에 미치는 영향

  • Shin, Jong-Wook (Department of Food Industrial Technology, Food Science and Technology, Catholic University of Daegu) ;
  • Lee, Sang-Il (Department of Food Nutrition & Cookery, Keimyung College) ;
  • Kim, Soon-Dong (Department of Food Industrial Technology, Food Science and Technology, Catholic University of Daegu)
  • 신종욱 (대구가톨릭대학교 외식식품산업학부 식품가공학) ;
  • 이상일 (계명문화대학 식품영양조리과) ;
  • 김순동 (대구가톨릭대학교 외식식품산업학부 식품가공학)
  • Published : 2008.10.31
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Abstract

The effects of acetic acid fermented juice prepared with submerged culture media of Antrodia camphorata mycelium (AJA: pH 3.2, acidity 2.0, Brix degree 3.2) on blood glucose levels and serum lipid profiles of rats in which diabetes was induced with streptozotocin (STZ) were investigated. Rats were divided into normal controls (NC), diabetic controls (DM), and groups receiving diluted (1:1, with water) AJA (A1) and undiluted AJA (A2). The volume of liquid given to both A1 and A2 animals was 0.5 mL/100 g body weight. In the A1 and A2 groups, compared with the DM group, polyphagia, liver enlargement, and weight loss caused by diabetes were considerably alleviated, but did not attain the levels of NC group rats. In the A1 and A2 groups, the levels of blood glucose were 17.1% and 28.2% lower than in the DM group. There was no significant difference in the levels of fructosamine between the DM and A1 group, but the A2 group had a level 16.3% lower than the DM group. In the A1 and A2 groups, compared with the DM group, serum triglyceride levels decreased by 44.1-48.0%, serum total cholesterol by 24.0-31.1%, and serum LDL-cholesterol by 25.2-51.1%. The level of HDL-cholesterol in A2 animals rose by 45.9% compared to NC rats. The results show that AJA may be a useful beverage for diabetes patients, offering both antihyperglycemic activity and improvement in levels of serum lipids.

우장지버섯 균사체 배양액으로 발효시킨 초산발효액 (AJA: pH 3.2, acidity 2.0, brix degree 3.2)이 streptozotocin으로 유발한 당뇨쥐의 혈당과 혈청지질에 미치는 영향을 조사하였다. 실험용 식이는 AIN-76 diet를 기본으로 하여 조제하였으며 실험동물은 5주령의 평균체중이 $140{\pm}10\;g$인 Sprague-Dawly계 흰쥐를 사용하여 정상대조군(NC), 당뇨 대조군(DM), 당뇨유발 후 체중 100 g당 AJA 1/2 희석액 0.5 mL 경구투여군(A1) 및 당뇨유발 후 체중 100 g당 AJA 0.5 mL 경구투여군(A2)의 4군으로 나누어 5주간 경구 투여하였다. A1 및 A2군은 DM군에 비하여 당뇨에 의하여 나타나는 다음 및 체중감소현상과 간의 비대현상이 완화되었다. A1 및 A2군의 5주째 혈당은 DM군에 비하여 17.1 및 28.2%가 감소되었으며, fructosamine의 함량은 A1군에서는 뚜렷한 차이가 없었으나 A2군에서는 16.3%가 감소되었다. A1 및 A2군은 DM군에 비하여 혈중 triglyceride 함량은 $44.1{\sim}48.0%$가, total cholesterol 함량은 $24.0{\sim}31.1%$가, LDL-cholesterol 함량은 $25.2{\sim}51.1%$가 각각 감소하였으며, HDL-cholesterol 함량은 A1군에서는 유의차가 없었으나 A2군에서는 45.9%가 증가하였다. 이상의 결과, 우장지버섯 균사체배양액으로 제조한 초산발효액은 STZ로 유도한 고혈당을 완화시키는 항당뇨작용과 혈중지질대사를 개선 시켜주는 효과가 있어 앞으로의 활용이 기대된다.

Keywords

References

  1. Tsai, Z.T. and Liaw, S.L. (1982) The use and the effect of Ganoderma, Sheng-Tun Publisher, Taichung, Taiwan, p.116-117
  2. Liu, D.Z., Liang, H.J., Chen, C.H., Su, C.H., Lee, T.H., Huang, C.T., Hou, W.C., Lin, S.Y., Zhong, W.B., Lin, P.J., Hung, L.F. and Liang, Y.C. (2007) Comparative anti-inflammatory characterization of wild fruiting body, liquid-state fermentation and solid-state culture of Taiwanofungus camphoratus in microglia and the mechanism of its action. J. Ethnopharmacol., 114, 78-85 https://doi.org/10.1016/j.jep.2007.07.028
  3. Rao, Y.K., Fang, S.H. and Tzeng, Y.M. (2007) Evaluation of the anti- inflammatory and anti-proliferation tumoral cells activities of Antrodia camphorata, Cordyceps sinensis and Cinnamomum osmophloeum bark extracts. J. Ethnopharmacol., 114, 78-85 https://doi.org/10.1016/j.jep.2007.07.028
  4. Shen, Y.C. (2004) Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with water-soluble fractions from five different Cinnamomum species. FEMS Microbiol. Lett., 231, 137-143 https://doi.org/10.1016/S0378-1097(03)00953-4
  5. Shen, Y.C., Wang, T.H. Chou, Y.C. Chen, C.F., Lin, L.C. and Chang, T.T. (2004) Evaluation of the anti- inflammatory activity of zhankuic acids isolated from the fruiting bodies of Antrodia camphorata. Planta Med., 70, 310-314 https://doi.org/10.1055/s-2004-818941
  6. Shena, Y.C., Choua, C.J., Wangb, Y.H., Chena, C.F., Chouc, Y.C. and Lu, M.K. (2004) Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with water-soluble fractions from five different Cinnamomum species. FEMS Microbiol. Lett., 231, 137-143 https://doi.org/10.1016/S0378-1097(03)00953-4
  7. Hseu, Y.C., Wu, F.Y., Wu, J.J., Chen, J.Y., Chang, W.H., Lu, F.J., Lai, Y.C. and Yang, H.L. (2005) Anti- inflammatory potential of Antrodia Camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-nB pathway. Int. J. Immunopharmacol., 5, 1914-1925 https://doi.org/10.1016/j.intimp.2005.06.013
  8. Shen, C.C., Yang, H.C., Huang, R.L., Chen, J.C. and Chen, C.C. (2005) Anti-HBV principle from the culture broth of Antrodia camphorota(STRAIN #CCRC-35396). J. Chin. Med., 16, 57-61
  9. Chen, C.H., Yang, S.W. and Shen, Y.C. (1995) New steroid acids from Antrodia cinnamomea, a fungal parasite of Cinnamomum micranthum. J. Nat. Prod., 58, 1655-1661 https://doi.org/10.1021/np50125a002
  10. Yang, H.L., Chen, C.S., Chang, W.H., Lu, F.J., Lai, Y.C., Chen, C.C., Hseu, T.H., Kuo, C.T. and Hseu, Y.C. (2006) Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by Antrodia camphorata. Cancer Lett., 231, 215-227 https://doi.org/10.1016/j.canlet.2005.02.004
  11. Wu, C.Y. and Liang, Z.C. (2005) Antimicrobial activity of extract from Antrodia camphorata cultured on pearl barley by solid-state fermentation. Taiwanese J. Agric. Chem. Food Sci., 43, 295-303
  12. Cheng, P.C., Hsu, C.Y., Chen, C.C. and Lee, K.M. (2008) In vivo immunomodulatory effects of Antrodia camphorata polysaccharides in a T1/T2 doubly transgenic mouse model for inhibiting infection of Schistosoma mansoni. Toxicol. Appl. Pharm., 277, 291-298
  13. Nakamura, N., Hirakawa, A., Gao, J.J., Kakuda, H., Shiro, M., Komatsu, Y., Sheu, C. and Hattori, M. (2004) Five new maleic and succinic acid derivatives from the mycelium of Antrodia camphorata and their cytotoxic effects on LLC tumor cell line. J. Nat. Prod., 67 46-48 https://doi.org/10.1021/np030293k
  14. Liu, J.J., Huang, T.S., Hsu, M.L., Chen, C.C., Lin, W.S., Lu, F.J. and Chang, W.H. (2004) Antitumor effects of the partially purified polysaccharides from Antrodia camphorata and the mechanism of its action. Toxicol. Appl. Pharmacol., 201, 186-193 https://doi.org/10.1016/j.taap.2004.05.016
  15. Im, J.G., Kang, M.S., Park, I.K. and Kim, S.D. (2005) Dietary effect of Liripis tuber water extracts on the level of blood glucose and werum cholesterol in streptozotocin- induced diabetic rat. J. East Asian Soc. Dietary., 15, 20-28
  16. Johnson, R.N., Metcalf, P.A. and Baker, J.R. (1982) Fructosamine: a new approach to the estimation of serum glycosylprotein. An index of diabetic control. Clin. Chim. Acta, 127, 87-95 https://doi.org/10.1016/0009-8981(83)90078-5
  17. Friedwald, W.T., Levy, R.I. and Fredrickson, D.S. (1972) Estimation of the concentration of the low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin. Chem., 18, 499-502
  18. Smith, O.L.K., Wong, C.Y. and Gelfand, R.A. (1989) Skeletal muscle proteolysis in rat with acute streptozotocin-induced diabetes. Diabetes, 38, 1117-1122 https://doi.org/10.2337/diabetes.38.9.1117
  19. Park, K.S., Lee, D.E., Shung, J.H. and Chung, S.H. (2002) Comparisons of antidiabetic effect of panax ginseng on MLD STZ-induced diabetic rats terms of time of administration. J. Ginseng Res., 26, 191-195 https://doi.org/10.5142/JGR.2002.26.4.191
  20. Grey, N.J., Krals, I. and Kipnis, D.M. (1975) Physiological mechanism in the development of starvation ketosis in man. Diabetes, 24, 10-14 https://doi.org/10.2337/diabetes.24.1.10
  21. Mogensen C.E., Anderson M,J,F. (1973) Increased kidney size and glomerular filtration rate early jurvenile diabetes. Diabetes, 22, 706-712 https://doi.org/10.2337/diab.22.9.706
  22. Ostman E., Granfeldt Y., Persson L. and Bjorck I. (2005) Vinegar supplementation lowers glucose and insulin responses and increases satiety after a bread meal in healthy subjects. Clin. Nutr., 59, 983-988 https://doi.org/10.1038/sj.ejcn.1602197
  23. Jang, K.H., Shin, K.O., Kim, S.D. (2005) Free amino acid and polysaccharide content of submerged mycelial culture of Fomitopsis pinicola. Korean J. Food Preserv., 12, 379-386
  24. Berrone, E., Beltramo, E., Solimine, C., Ape, A.U. and Porta, M. (2006) Regulation of intracellular glucose and polyol pathway by thiamine and benfotiamine in vascular cells cultured in high glucose. J. Biol. Chem., 281, 9307-9313 https://doi.org/10.1074/jbc.M600418200
  25. Kawasumi, M., Tanaka, Y., Uchino, H., Shimizu, T., Tamura, Y., Sato, F., Mita, T., Watada, H., Sakai, K., Hirose, T. and Kawamori, R. (2006) Strict glycemic control ameliorates the increase of carotid IMT in patients with type 2 diabetes. J. Endocrinol. 53, 45-50
  26. Demetrious, J.A. (1987) Glycosylated proteins. 'Methods in Clinical Chemistry' (Pesce AJ, Kaplan LA eds), Mosby Co. Washington DC, USA., p.113-123
  27. Tall, A.R. (1990) Plasma high density lipoproteins metabolism and relationship to atherogenesis. J. Clin. Invest., 86, 379-384 https://doi.org/10.1172/JCI114722
  28. Jones, I.R., Owens, D.R., Williams, S., Ryder, R.E., Birtwell, A.J., Jones, M.K., Gicheru, K. and Hayes, T.M. (1983) Glycosylated serum albumin: an intermediate index of diabetic control. Diabetes Care, 6, 501-503 https://doi.org/10.2337/diacare.6.5.501
  29. Lee, I.S., Lee, S.W. and Lee, I.Z. (2003) Effects of tissue cultured ginseng on blood glucose and lipid in streptozotocin-induced diabetic rats. Korean J. Food Sci. Technol., 35, 280-285
  30. Jung, S.H., Kim, J.H., Jeong, Y.J. and Choi, M.J. (1999) Effect of persimmon vinegar on serum lipid profile in rats with high cholesterol diet. J. East Asian Soc. Dietary Life, 9, 421-426