Microbiological Contamination Level and Radiation Sterilization in Disposable Kitchen Utensil

일회용 주방용품의 미생물 오염도평가 및 방사선 살균

  • Kim Jin-Hee (Radiation Food Science & Biotechnology Team, Korea Atomic Energy Research Institute) ;
  • Lim Sang-Yong (Radiation Food Science & Biotechnology Team, Korea Atomic Energy Research Institute) ;
  • Song Hyun-Pa (Radiation Food Science & Biotechnology Team, Korea Atomic Energy Research Institute) ;
  • Kim Byeong-Keun (Radiation Food Science & Biotechnology Team, Korea Atomic Energy Research Institute) ;
  • Chung Jin-Woo (Radiation Food Science & Biotechnology Team, Korea Atomic Energy Research Institute) ;
  • Yoon Hae-Jung (Radiation Food Science & Biotechnology Team, Korea Atomic Energy Research Institute) ;
  • Byun Myung-Woo (Radiation Food Science & Biotechnology Team, Korea Atomic Energy Research Institute) ;
  • Kim Dong-Ho (Radiation Food Science & Biotechnology Team, Korea Atomic Energy Research Institute)
  • 김진희 (한국원자력연구소 방사선식품생명공학 기술개발팀) ;
  • 임상용 (한국원자력연구소 방사선식품생명공학 기술개발팀) ;
  • 송현파 (한국원자력연구소 방사선식품생명공학 기술개발팀) ;
  • 김병근 (한국원자력연구소 방사선식품생명공학 기술개발팀) ;
  • 정진우 (한국원자력연구소 방사선식품생명공학 기술개발팀) ;
  • 윤혜정 (한국원자력연구소 방사선식품생명공학 기술개발팀) ;
  • 변명우 (한국원자력연구소 방사선식품생명공학 기술개발팀) ;
  • 김동호 (한국원자력연구소 방사선식품생명공학 기술개발팀)
  • Published : 2005.08.01

Abstract

The purposes of this study were to assess the microbiological contamination level of various disposable kitchen utensils and evaluate the effectiveness of gamma irradiation as a sterilization process for the utensils. The 51 kinds in 17 groups of disposable kitchen utensils were tested for the enumeration of total aerobic plate count, coliforms, fungi and Salmonella. Generally, cell density of microorganisms in disposable kitchen utensils were lower than that of cooking utensils currently using in kitchen. The survivals of total aerobic plate counts, putrefactive bacteria, on the surface of the disposable utensils were ranged up to $10^3\;CFU/100\;cm^2$. Filamentous fungi were detected in 13 samples. Coliform bacteria were detected in two kinds of samples but Salmonella spp. was detected below detection limits in all the samples. The microorganisms survived on the surface of utensils were effectively eliminated by gamma irradiation process at 3 kGy.

일회용 주방용품의 미생물학적 안전성 확보를 위한 연구의 일환으로 17개 제품군, 총 51종의 일회용 주방용품에 대한 미생물 오염도 평가와 함께 오염된 미생물을 효과적으로 살균할 수 있는 방안으로 감마선 살균 방법을 적용하였다. 일반호기성세균, 대장균군, 곰팡이 Salmonella의 오염도 평가 결과, 대장균군과 Salmonella는 거의 검출되지 않았으나 일반호기성 세균은 전체 시료의 약$50\%$에서 $10^1{\sim}10^2\;CFU/100\;cm^2$ 수준으로 검출되어 일회용 주방용품의 위생화가 필요함을 확인하였다. 일회용 주방용품에 오염된 미생물의 감마선 살균 결과 3 kGy의 조사선량에서 미생물이 검출되지 않았다.

Keywords

References

  1. Sobel, J., Griffin, P.M., Slutsker, L., Swerdlow, D.L and Tauxe, R. V. (2002) Investigation of mulristate foodbome disease outbreak. Public Health Reports, 117, 8-19
  2. Sun, Y.M. and Ockerman, H.W. (2005) A review of the needs and current applications of hazard analysis and critical control point (HACCP) system in foodservice areas. Food Control, 16, 325-332 https://doi.org/10.1016/j.foodcont.2004.03.012
  3. Todd, E.C.D. (2003) Microbiological safety standards and public health goals to reduce foodbome disease. Meat Sci., 66, 33-43 https://doi.org/10.1016/S0309-1740(03)00023-8
  4. Scott, E. and Bloomfield, S.F. (1990) The survival and transfer of microbial contamination via cloths, hands and utensils. J. Appl. Bacteriol, 68, 271-278 https://doi.org/10.1111/j.1365-2672.1990.tb02574.x
  5. Beumer, R.R. and Kusumaningrum, H. (2003) Kitchen hygiene in daily life. International Biodeterioration & Biodegradation, 51, 299-302 https://doi.org/10.1016/S0964-8305(03)00041-6
  6. Chun, H.J., Paik, J.E, Lee, Y.K. and Kim, E.S. (1998) The microbiological assessment of plastic container and kitchen utensils used in employee feeding foodservice operation in seoul. Korean J. Soc. Food Sci., 14, 21-24
  7. Park, H.K., Kim, K.L., Shin, H.W., Kye, S.H. and Yoo, W.C. (2000) Evaluation of microbiological harzards of cooking utensils and environment of mass catering establishments. J. Fd Hyg. Safety, 15, 315-323
  8. Kusmnaningrum, H.D., Riboldi, G., Hazeleger, W.C. and Beumer, R.R. (2003) Survival of foodbome pathogens on stainless steel surfaces and cross-contamination to foods. International J. Food Microbiology, 85, 227-236 https://doi.org/10.1016/S0168-1605(02)00540-8
  9. Chen, Y.H., Jackson, K.M., Chea, F.P. and Schaffner, D.W. (2001) Quantification and variability analysis of bacterial cross-contamination rates in common food service tasks. J. Food Protec., 64, 72-80
  10. Gorman, R., Bloomfield, S. and Adley, C.C. (2002) A study of cross contamination of food-borne pathogens in the domestic kitchen in the Republic of Ireland. International J. Food Microbiology, 76, 145-150
  11. Zhao, P., Zhao, T., Doyle, M.P., Rubino, J.R. and Meng, J. (1998) Development of a model for evaluation of microbial cross contamination in the kitchen. J. Food Protection, 61, 960-963
  12. Pasquarella, C., Pitzurra, O. and Savino, A. (2000) The index of microbial air contamination. Journal of Hospital Infection, 46, 241-256 https://doi.org/10.1053/jhin.2000.0820
  13. FAO/IAEA/WHO Study Group. (1999) High-dose irradiation: Wholesomeness of food irradiated with doses above 10 kGy. In WHO tedmical report series 890. World Health Organization, Geneva. p.49- 77
  14. Byun. M.W. (1997) Application and aspect of irradiation technology in food industry. Food Sci. Ind, 30, 89-100
  15. Thayer, D.W. (1990) Food irradiation, Benefits and concerns. J. Food Quality, 13, 147-169 https://doi.org/10.1111/j.1745-4557.1990.tb00014.x
  16. Difco Laboratories. (1984) Difco manual. 10th ed. Detroit, Michigan, USA
  17. Bhattacharya, A. (2000) Radiation and industrial polymers. Prog. Polym. Sci., 25, 371-401 https://doi.org/10.1016/S0079-6700(00)00009-5
  18. Davenas, J., Stevenson, I., Celette, N., Cambon, S., Gardette, J.L., Rivaton, A. and Vignoud, L. (2002) Stability of polymers under ionising radiation: The many faces of radiation interactions with polymers. Nucl. Instr. and Meth. in Phys. Res., B 191, 653-661 https://doi.org/10.1016/S0168-583X(02)00628-6