• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.2
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• pp.149-154
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• 2005

Secretion of the expressed heterologous proteins can reduce the stress to the host cells and is beneficial to their recovery and purification. In this study, fed-batch cultures of Escherichia coli YK537 (pAET-8) were conducted in a 5-L fermentor for the secretory production of human epidermal growth factor (hEGF) whose expression was under the control of alkaline phosphatase promoter. The effects of feeding of glucose and complex nitrogen sources on hEGF production were investigated. When the fed-batch culture was conducted in a chemically de-fined medium, the cell density was 9.68 g/L and the secreted hEGF was 44.7 mg/L in a period of 60 h. When a complex medium was used and glucose was added in pH-stat mode, the secreted hEGF was improved to 345 mg/L. When the culture was fed with glucose at a constant specific rate of $0.25\;gg^{-1}h^{-1}$, hEGF reached 514 mg/L. The effects of adding a solution containing yeast extract and tryptone were further studied. Different rate of the nitrogen source feeding resulted in different levels of phosphate and acetic acid formation, thus affected hEGF expression. At the optimal feeding rate, hEGF production achieved 686 mg/L.

• Membrane and Water Treatment
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• v.8 no.5
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• pp.449-462
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• 2017

Direct treatment of municipal wastewater by forward osmosis (FO) process was evaluated in terms of water flux decline, reverse salt diffusion, pollutants rejection and concentration efficiency by using synthetic seawater as the draw solution. It was found that when operating in PRO mode (active layer facing the draw solution), although the FO membrane exhibited higher osmotic water flux, more severe flux decline and reverse salt diffusion was also observed due to the more severe fouling of pollutants in the membrane support layer and accompanied fouling enhanced concentration polarization. In addition, although the water flux decline was shown to be lower for the FO mode (active layer facing the feed solution), irreversible membrane fouling was identified in both PRO and FO modes as the water flux cannot be restored to the initial value by physical flushing, highlighting the necessity of chemical cleaning in long-term operation. During the 7 cycles of filtration conducted in the experiments, the FO membrane exhibited considerably high rejection for TOC, COD, TP and $NH_4{^+}-N$ present in the wastewater. By optimizing the volume ratio of seawater draw solution/wastewater feed solution, a concentration factor of 3.1 and 3.7 was obtained for the FO and PRO modes, respectively. The results demonstrated the validity of the FO process for direct treatment of municipal wastewater by using seawater as the draw solution, while facilitating the subsequent utilization of concentrated wastewater for bioenergy production, which may have special implications for the coastline areas.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37 no.6B
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• pp.423-432
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• 2012

We design a control circuit that can switch input power between a rechargeable battery and a sensor communication device (mote) depending on the operating state of a solar cell as well as the active and sleep mode of a sensor MAC protocol. A mote that simply combines a solarcell and a rechargeable battery may die if there is not sunlight long. A battery is recharged if sunlight is sufficient and a device is in a sleep mode, and it supplies power if sunlight is low and the mote is in an active mode. A mote can switch its input power between solar cell and battery depending on the output level of a solar cell. During this switching, a mote may lose its state information due to the reset of a microprocessor by the transient power-off. A capacitor is used to cope with this phenomenon and also supplies power to a mote during a sleep mode. Experimental results show that the solar cell based mote operates in a very stable manner against the lack of sunlight long.

• Journal of the Korean Society for Precision Engineering
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• v.24 no.11
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• pp.91-100
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• 2007

Many manufacturing devices must execute motions as quickly as possible to achieve profitable high-volume production. Most of them have devices having flexibility and a time delay of one sampling is added to the plants when they are controlled by fast discrete controllers, which brings about non-minimum phase zeros. This paper develops a control strategy that combines feedforward and feedback control with command shaping for such devices. First, the feedback controller is designed to increase damping and eliminate steady-state error. Next, the feedforward controller is designed to speed up the transient response. Finally, an appropriate reference profile is generated using command-shaping techniques to ensure fast point-to-point motions with minimum residual vibration. The particular focus of the paper is to understand the interactions between these individual control components. The resulting control strategy is demonstrated on a model of a high-speed semiconductor manufacturing machine.

• Proceedings of the KWS Conference
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• 2002.10a
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• pp.176-180
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• 2002

During the last two decades the laser beam has progressed from a sophisticated laboratory apparatus to an adaptable and viable industrial tool. Especially, in its welding mode, the laser offers high travel speed, low distortion, and narrow fusion and heat-affected zones (HAZ). The principal obstacle to selection of a laser processing method in production is its relatively high equipment cost and the natural unwillingness of production supervision to try something new until it is thoroughly proven. The major objective of this work is focused on the combined features of gas tungsten arc and a low-power cold laser beam. Although high-power laser beams have been combined with the plasma from a gas tungsten arc (GTA) torch for use in welding as early as 1980, recent work at the Ohio State University has employed a low power laser beam to initiate, direct, and concentrate a gas tungsten arcs. In this work, the laser beam from a 7 watts carbon monoxide laser was combined with electrical discharges from a short-pulsed capacitive discharge GTA welding power supply. When the low power CO laser beam passes through a special composition shielding gas, the CO molecules in the gas absorbs the radiation, and ionizes through a process known as non-equilibrium, vibration-vibration pumping. The resulting laser-induced plasma (LIP) was positioned between various configurations of electrodes. The high-voltage impulse applied to the electrodes forced rapid electrical breakdown between the electrodes. Electrical discharges between tungsten electrodes and aluminum sheet specimens followed the ionized path provided by LIP. The result was well focused melted spots.

• Journal of Drive and Control
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• v.14 no.2
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• pp.45-51
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• 2017

Semi-submersible drilling rigs are offshore plants that perform functions such as ocean exploration for oil and gas acquisition, drilling and production, and storage and unloading of crude oil and gas. Semi-submersible drilling rigs use watertight dampers as emergency buoyancy holders. Since the watertight damper is an emergency shutoff device, it is mainly driven by a pneumatic driving system that can operate without a power supply. The pneumatic driving system has highly non-linear characteristics due to compressibility of air and external disturbance such as static and Coulomb friction. In this paper, a new control algorithm is proposed for a watertight damper driving system based on the sliding mode control with a disturbance observer. To evaluate control performance and robust stability of the designed controller, the control results were compared with the results obtained using the state feedback controller. As a result, it was confirmed that the pneumatic driving system for driving the watertight damper using the sliding mode controller with a disturbance observer can obtain excellent control performance against the parameter changes and the disturbance input.

• ALGAE
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• v.28 no.2
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• pp.131-147
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• 2013

Major progress has been made in the past decade towards understanding of the biosynthesis of red carotenoid astaxanthin and its roles in stress response while exploiting microalgae-based astaxanthin as a potent antioxidant for human health and as a coloring agent for aquaculture applications. In this review, astaxanthin-producing green microalgae are briefly summarized with Haematococcus pluvialis and Chlorella zofingiensis recognized to be the most popular astaxanthin-producers. Two distinct pathways for astaxanthin synthesis along with associated cellular, physiological, and biochemical changes are elucidated using H. pluvialis and C. zofingiensis as the model systems. Interactions between astaxanthin biosynthesis and photosynthesis, fatty acid biosynthesis and enzymatic defense systems are described in the context of multiple lines of defense mechanisms working in concert against photooxidative stress. Major pros and cons of mass cultivation of H. pluvialis and C. zofingiensis in phototrophic, heterotrophic, and mixotrophic culture modes are analyzed. Recent progress in genetic engineering of plants and microalgae for astaxanthin production is presented. Future advancement in microalgal astaxanthin research will depend largely on genome sequencing of H. pluvialis and C. zofingiensis and genetic toolbox development. Continuous effort along the heterotrophic-phototrophic culture mode could lead to major expansion of the microalgal astaxanthin industry.

• Animal Bioscience
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• v.37 no.1
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• pp.1-15
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• 2024

Poultry coccidiosis is an intestinal infection caused by an intracellular parasitic protozoan of the genus Eimeria. Coccidia-induced gastrointestinal inflammation results in large economic losses, hence finding methods to decrease its prevalence is critical for industry participants and academic researchers. It has been demonstrated that coccidiosis can be effectively controlled and managed by employing anticoccidial chemical compounds. However, as a result of their extensive use, anticoccidial drug resistance in Eimeria species has raised concerns. Phytochemical/herbal medicines (Artemisia annua, Bidens pilosa, and garlic) seem to be a promising strategy for preventing coccidiosis, in accordance with the "anticoccidial chemical-free" standards. The impact of herbal supplements on poultry coccidiosis is based on the reduction of oocyst output by preventing the proliferation and growth of Eimeria species in chicken gastrointestinal tissues and lowering intestinal permeability via increased epithelial turnover. This review provides a thorough up-to-date assessment of the state of the art and technologies in the prevention and treatment of coccidiosis in chickens, including the most used phytochemical medications, their mode of action, and the applicable legal framework in the European Union.

• Journal of the Korean Regional Science Association
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• v.11 no.1
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• pp.79-91
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• 1995

As an exit to solve the economic depression of the development countries in the early twentieth century, the 'old international division of labor' developed. The economic crisis(i.e., under-consumption crisis) was due to the absence of the mode of regulation compatible with the extensive regime of accumulation(i.e., "Fordist" regime). The crisis was solved by the state intervention through the creation on institutions in order to increase the level of consumption. Until the late 1960s when "high Fordism" reached(i.e., a harmonious relation between the monopoly mode of regulation and the intensive accumulation of capital), the developed core countries enjoyed a remarkable economic growth. The external market was not a necessity for the economic growth because there were increases in labor productivity and proportional increases in real wages and thus increases in consumption level. In the 1970s, however, the core faced with economic crisis again. Due to the breakdown of the postwar "Fordist" regime of capital accumulation and the post 1973 world depression, the core needed the Third World as a solution for their internal and international economic crisis. Thus the 'new international division of labor'(NIDL) arose. The "Fordist" method of production(i.e., the divisions of production process) led to the territorial division of labor and to the detailed division of labor. The aim of the NIDL is to exploit reserve armies of labor on a world scale and thus to reduce production costs. According to the NIDL model, the Third World countries have been developing by the core countries' investment on mainly labor-intensive industries and thus have been playing an important role in the global economy. And the NIDL theorists argue that multinational corporations have increasingly invested in the Third World nations and contributed to the economic growth in those regions. Tables presented in the paper show that the global trend since the 1970s does not follow the argument exactly as the NIDL theorists predicted. On the contrary, the core countries focus on developing technology, adopting the automation of production process, and trading within the core countries rather than on investing in the periopheral countries. The continuing investment of multinational corporations into the periphery is not because of cheap labor force but because of the market potentials in the regions. Majority of corporations of the core tries to reduce production costs by investing in technological development more intensively and also by changing regional strategies (i.E., investment from metropolitan areas to medium - or small - size cities, focusing on agglomeration economy, boosting regional diversification, etc.) within their own countries. The main purpose of the paper is to review and to criticize the NIDL theory based on some empirical data.IDL theory based on some empirical data.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.8
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• pp.1210-1214
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• 2002

The large and rapid changes of glucose utilization in lactating mammary tissue in response to changes in nutritional state must be largely related by external signal of insulin. This also must be related with the quantity and composition of the diet in vivo. To characterize the mode of gut extract protein with insulin, in vitro experiment was conducted with HC11 cells. The gut extract protein has not only the same effect as insulin alone but also the synergistic effect with insulin in 2-Deoxy[3H] glucose uptake. Although the gut extract did not modulates glucose uptake via increasing the rate of translation of the GLUT1 protein, northern blot analysis indicated that the gut extract protein increased the expression of GLUT1 mRNA by a threefold and also there was a dose-dependent increase in the expression of GLUT1 mRNA. The gut extract protein is therefore shown to be capable of modulating glucose uptake by transcription level with insulin in HC 11 cells.