• Journal of Environmental Impact Assessment
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• v.24 no.6
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• pp.607-618
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• 2015

Five years have passed since the first set of environmental samples was taken in 2011 to represent various ecosystems which would help future generations lead back to the past environment. Those samples have been preserved cryogenically in the National Environmental Specimen Bank(NESB) at the National Institute of Environmental Research. Even though there is a strict regulation (SOP, standard operating procedure) that rules over the whole sampling procedure to ensure each sample to represent the sampling area, it has not been put to the test for the validation. The question needs to be answered to clear any doubts on the representativeness and the quality of the samples. In order to address the question and ensure the sampling practice set in the SOP, many steps to the measurement of the sample, that is, from sampling in the field and the chemical analysis in the lab are broken down to evaluate the uncertainty at each level. Of the 8 species currently taken for the cryogenic preservation in the NESB, pine tree samples from two different sites were selected for this study. Duplicate samples were taken from each site according to the sampling protocol followed by the duplicate analyses which were carried out for each discrete sample. The uncertainties were evaluated by Robust ANOVA; two levels of uncertainty, one is the uncertainty from the sampling practice, and the other from the analytical process, were then compiled to give the measurement uncertainty on a measured concentration of the measurand. As a result, it was confirmed that it is the sampling practice not the analytical process that accounts for the most of the measurement uncertainty. Based on the top-down approach for the measurement uncertainty, the efficient way to ensure the representativeness of the sample was to increase the quantity of each discrete sample for the making of a composite sample, than to increase the number of the discrete samples across the site. Furthermore, the cost-effective approach to enhance the confidence level on the measurement can be expected from the efforts to lower the sampling uncertainty, not the analytical uncertainty. To test the representativeness of a composite sample of a sampling area, the variance within the site should be less than the difference from duplicate sampling. For that, a criterion, ${i.e.s^2}_{geochem}$(across the site variance) <${s^2}_{samp}$(variance at the sampling location) was proposed. In light of the criterion, the two representative samples for the two study areas passed the requirement. In contrast, whenever the variance of among the sampling locations (i.e. across the site) is larger than the sampling variance, more sampling increments need to be added within the sampling area until the requirement for the representativeness is achieved.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.2
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• pp.33-44
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• 2012

Transparent exopolymer particles (TEP) are formed by aggregation of polysaccharide products excreted by phytoplankton and have sticky character like gel. They play important role in the production of marine snow in water column. To study the distribution pattern of TEP concentration and its role in carbon cycle in the surface ocean, we measured pH, Total alkalinity (TA), and chlorophyll-a in addition to physical characteristics of seawater within the surface water column. TEP concentrations ranged from nearly undetectable values to $338{\mu}g\;Xeq\;l^{-1}$. They were considerably lower than previously reported values from costal sites, but showed similar values observed in other oceanic region during phytoplankton bloom periods. The spatial distribution of TEP concentrations were similar to those of chlorophyll-a, which indicate that the production of TEP were closely related to phytoplankton. Calculated total dissolved inorganic carbon ($TCO_2$) from the pH and TA was normalized to 35 psu of salinity ($NTCO_2$) and showed negative linear relationship with temperature. Biological drawdown of $NTCO_2$ ($NTCO_{2bio}$) was estimated from the difference between theoretical $NTCO_2$ values and observed $NTCO_2$. In the warm region located south of $40^{\circ}N$ along the $132.5^{\circ}N$ meridional lines, $NTCO_{2bio}$ showed negative value and TEP concentrations were high. This suggested that negative $NTCO_{2bio}$ may be attributed to the biological processes. At the stations located between 44 and $46^{\circ}N$, TEP concentrations showed high concentration at the chlorophyll-a maximum layer within the water column while they showed low concentration in the surface layer. Carbon content of TEP constituted about 40% of $NTCO_{2bio}$ at the chlorophylla maximum layer. In this study, we could not observe any positive and negative relationship between TEP concentration and $NTCO_2$ or pH. It is obvious that we should consider the importance of TEP in the biological carbon cycling processes within surface layer.

• Journal of Korean Society of Forest Science
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• v.98 no.6
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• pp.772-779
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• 2009

This study was conducted to estimate the carbon (C) contents in pure and mixed stands of pine (Pinus densiflora) and oak (Quercus spp.) trees for establishing the C inventory of forest ecosystems. A total of fifteen 20 m${\times}$20 m pure and mixed stands of pine and oak trees were chosen in natural forests in Hoengseong, Kangwon based on the basal area of all trees ${\geq}$ 5 cm DBH: three of 95% of pine and 5% oak trees [pine stand], three of 100% of oak trees [oak stand], and nine of 20 to 70% of pine and 80 to 30% of oak trees [mixed stand]. To estimate C contents in the study stands, biomass in vegetation, forest floor and coarse woody debris (CWD) were calculated and C concentrations in vegetation, forest floor, CWD and soil (0-30 cm) were analyzed. There was no significant difference in vegetation C contents among the stands; 147.6 Mg C/ha for the oak stand, 141.4 Mg C/ha for the pine stand and 115.8 Mg C/ha for the mixed stand. Forest floor C contents were significantly different among the stands (p<0.05); 12.7 Mg/ha for the pine stand, 9.9 Mg/ha for the oak stand, and 8.4 Mg/ha for the mixed stand. However, CWD C contents were not significantly different among the stands (p>0.05); 2.2 Mg/ha for the mixed stand, 1.7 Mg/ha for the oak stand, and 1.1 Mg/ha for the pine stand. Soil C contents up to 30 cm depth were not significantly different among the study stands; 44.4 Mg C/ha for the pine stand, 41.6 Mg C/ha for the mixed stand, and 33.3 Mg C/ha for the oak stand. Total ecosystem C contents were lower in the mixed stand than those in the pure stands, because vegetation C contents which occupied almost total ecosystem C contents were lower in the mixed stand than those in the pure stands; 199.6 Mg C/ha for the pine stand, 192.5 Mg C/ha for the oak stand and 169.1 Mg C/ha for the mixed stand. Lower vegetation C contents in the mixed stand might be influenced by interspecific competition between pine and oak trees and intraspecific competition among the oak trees resulted from high stand density. We suggest that forest management such as thinning to enhance C storage is indispensible for minimizing the competition in forest ecosystems.

• Journal of Nutrition and Health
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• v.1 no.1
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• pp.9-18
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• 1968

Dividing into six(6) experimental groups; Stock Diet(SD) Group, Rice Diet(RD) Group, Rice Diet supplemented with Barley(RD-BD) Group, Barley Diet(BD) Group, Rice Diet supplemented with Millet(RD-MD) Group, and Millet Diet(MD) Group, biochemical and nutritional effects of rice, barley, and millet on rats are studied. The grains used in this experiment are polished ones and purchased from market. Six(6) weaning male albino rats are housed into the individual cages in each group and fed with each experimental diet and water ad libitum for 21 weeks. The composition of the experimental diets are shown in Table 1. At the end of experiment, the appearences of rats are observed and the bloods are sampled by heart puncture method following ether anesthesia for determinations of serum protein and serum cholesterol. And the livers are sampled immediately right after heart after heart puncture for determinations of liver weight, liver nitrogen, liver fat, and liver glycogen. And also liver tissues are observed histologically. The result of growth experiment is shown in Table 2 and Figure 7. The results of blood analysis are shown in Table 3 and the results of liver analysis are shown in Table 4. And the histological observations of liver tissue are shown in Figures 1, 2, 3, 4, 5, and 6. According to the above results, it could be summarized as follows; 1) The normal appearences are observed at SD Group and BD Group. Alopecia is observed at RD, RD-­BD, RD-MD, and MD groups and the wet appearences of hair are observed at RD-MD and MD groups. The color of hair is changed to yellowish white at RD, RD-MD groups. The maximum growth inhibition, is observed at MD group and the growths of RD, RD-BD, BD, and RD-MD groups are significantly lower than that of SD group. The growth of RD group is lower than those of RD-BD and BD groups, though this difference is not significant. 2) At the blood analysis, the highest serum protein content is shown at SD group and the lowest serum protein is observed at MD group. The serum cholesterol contents are not significantly different among the six(6) experimental groups. 3) The highest liver weight is observed at SD group and the lowest weight is shown at MD group. However, oppositely the highest liver weight per 100 grams of body weight at MD group and the lowest liver weight per 100 grams of body weight at SD group are observed as shown in Table 6. 4) The differences of liver nitrogen contents of RD, RB-BD, BD, RD-MD, and MD Groups are not significant except SD group showing the highest content. 5) The significant highest content of liver fat is observed at RD group and the lowest content of liver fat is shown at SD group. And no significant differences in contents of liver glycogen are observed among the six(6) experimental groups. 6) No significant abnormal histological changes of liver tissue are observed at SD, RD-BD, and BD groups. However, the fat metamorphosis at peripheral zone of liver tissue is observed at RD Group and the some histological abnormal changes are observed at RD-MD and MD groups. According to the above summaries, the followings might be estimated and recommended; 1) The BD group shows histological normality of liver tissue, improvement of liver fat and liver nitrogen, normality of appearence, and better growth compared with RD Group. This could be indicated that barley is nutritionally and biochemically better than rice as the feeding diet on rat. And it might be considered that there might be the species speciality for nutritionary evaluation of food. 2) Recently, Marshall indicated that the high consumption of carbohydrate causes fatty liver and Kondo reported that the higher consumption of rice causes the more heart disease and the shorter life span. Considering these reports, the higher consumption of rice as a main dish in our country should be considered as an important national nutritional problem. And this might be improved by supplementing with barley or other grains to rice.

• Journal of Bio-Environment Control
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• v.21 no.2
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• pp.147-152
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• 2012

This study was conducted to investigate the photosynthetic R. micranthum by natural habitats. In the results, natural habitats didn't affect values of light saturated point, light compensation point and photosynthetic capacity of R. micranthum. We investigated light response curve and chlorophyll content at each habitat. Light compensation points were 11.8 ${\mu}mol\;m^{-2}\;s^{-1}$, 11.5 ${\mu}mol\;m^{-2}\;s^{-1}$ and 10.4 ${\mu}mol\;m^{-2}\;s^{-1}$ in Seokpo-ri, Yeonha-ri, and Mt. Worak. Light saturation points showed that R. micranthum is shade tolerant specie which has the light saturation point approximately 500~600 ${\mu}mol\;m^{-2}\;s^{-1}$. Photosynthetic rates of R. micranthum leaves were 5.5 ${\mu}mol\;m^{-2}\;s^{-1}$, 5.4 ${\mu}mol\;m^{-2}\;s^{-1}$ and 5.6 ${\mu}mol\;m^{-2}\;s^{-1}$ in Seokpo-ri, Yeonha-ri and Mt. Worak. On the other hand, since between $20^{\circ}C$ and $30^{\circ}C$, it appeared that the values of net photosynthetic rates of R. micranthum leaves in all sites were high. Especially, the rates were highest at $25^{\circ}C$. Because of low stomatal transpiration rate in saturation radiance, the moisture utilization efficiency in Yeonha-ri was lower than other habitats. Rates of chlorophyll a, chlorophyll b, and total chlorophyll content in Mt. Worak were no significant difference. Therefore R. micranthum has characteristic of shade tolerant species. The moderate temperature for R. micranthum is between $20^{\circ}C$ and $30^{\circ}C$.

• Biomedical Science Letters
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• v.3 no.1
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• pp.55-67
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• 1997

Considering many problems caused by the abuse of antibiotics recently, the appearance of antibiotic resistance bacteria is believed to help the cure of patients greatly. From Jan. 1st, 1996 to Dec. 31, 1996, 6135 strains were examined after being asked of and seperated from the clinical pathology departments of general hospitals, and the isolation frequency of identified bacteria and the susceptibility of antibiotics showed the following result. 1. The isolation frequency of strains was Escherichia coli, 1134 strains (18.4%), Pseudomonas aeruginosa, 856 strains (13.9%), coagulase negative Staphylococcus, 793 strains (12.89%), Staphylococcus aureus, 555 strains (9.02%), B. cepacia, 421 strains (6.84%), Enterobacter cloacae, 366 strains (5.95%), Enterobacter faecalis (4.86%), and Klebsiella pneumonia, 220 strains (3.85%). 2. The isolation rate of specimen was urine, 1, 969 strains, wound 1, 104 strains, sputum 701 strains, blood 643 strains, vaginal swab, 342 strains, and eye discharge, 192 strains, 40% of urine strains were E. coli 18％ of wound strains were B. cepacia, 43.7％ of sputum were P. aeruginosa, and in blood strains there were Enterobacter cloacae (25.8%), coagulase negative Staphylococcus (19.6%), and P. aeruginosa (8.7%). 3. The result of antibiotics susceptibility showed that, among gram negative bacilli, P. aeruginoas had resistance in almost all antibiotics except ceftazidme imipenem. But B. cepacia, the same glucose non-fermentation gram negative bacilli had more than 90％ of sensitivity in aztreonam, ceftazidime, ciproflxacin, piperacillin, trimethoprim/sulfa and had resistance in the others. Enterococcus faecalis showed more than 85% of sensitivity in penicillin-G, ampicillin, ciprofloxacin. 4. In the case of specimen antibiotics susceptibility, Enterobacter cloacae was lower in specimen isolated from blood than in those isolated from others and p. aeruginosa was low in specimen isolated from urine, which showed that there was difference in specimen antibiotics susceptibility. The result of this study shows that there happen many resisitances in antibiotics used frequently and some countermeasure is necessary because many bacteria began to show new resistance. Also it is desirable that the choice of antibiotics for infection diagnosis and its cure should be made after the inspection of antibiotics.

• Journal of Wetlands Research
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• v.19 no.2
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• pp.252-258
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• 2017

To determine the effects of small dam on benthic macro-invertebrate community, benthic macro-invertebrates were surveyed with both quantitative and qualitative collecting methods from 4 sites at a terraced interval (3.02 small dams per 100 m) and from 5 sites at a non-terraced interval (0.96 small dams per 100 m) in upper reach of Dorim stream, Seoul. The average water flow rate was higher at the non-terraced interval (0.149 m/s) than at the terraced interval (0.043 m/s). Diptera, which prefer slow water flow or lentic habitats, appeared more abundantly in the terraced interval (78.3%) than in the non-terraced interval (32.0%)(p<.05). Ephemeroptera, which prefer fast water flow or lotic habitats, appeared more abundantly in the non-terraced interval (14.1%) than in the terraced interval (4.5%)(p=.203). There was a difference in species distribution of Trichoptera in terraced and non-terraced intervals depending on preferred environment. In the terraced interval, the occupancy ratio of gathering-collector was 1.8 times higher than in the non-terraced interval (p<.05). In contrast, filtering-collector appeared to be about 4.4 times higher rate in the terraced interval (p=.124). The evenness indexes (J′) were found to be 0.41 and 0.77, respectively in terraced area and non-terraced intervals. KSI, evaluating biological water quality, showed an average of 4.0 and 2.4 respectively in terraced and non-terraced intervals. EPT/C index, indicating the balance of the community, showed an average of 0.1 and 0.7 respectively in terraced and non-terraced intervals. It suggests that we should make small dams in order to maintain biodiversity of benthic macro-invertebrates.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.318-331
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• 1996

Large and small seeds (44 and 22 mg per caryopsis) of a spring wheat (cv. Kulin) and a spring barley (cv. Skiff) were sown at two nitrogen rates (equivalent to 10 and 32 g m$^{-2}$ ) in well-watered pots under outdoor conditions to determine the effects of seed size and nitrogen (N) nutrition on water use efficiency (WUE) and carbon isotope discrimination ($\Delta$) and to evaluate interaction among $\Delta$, WUE and N nutrition in wheat and barley. Barley produced, on average, 105% more biomass (root＋shoot dry weight) than wheat at stem elongation because of early vigor. By anthesis this difference had disappeared as wheat had 16% more biomass than barley which headed 3 days earlier. Compared to plants grown from small seed, plants grown from large seed had much greater biomass in wheat than in barley at stem elongation and anthesis. Higher N nutrition increased average biomass of wheat and barley by 40 and 31%, respectively, at anthesis. Barley had 35 and 20% greater WUE (biomass gained/transpiration) than wheat at stem elongation and anthesis, respectively, and 2.0 to 3.6% lower $\Delta$ in aboveground shoots depending on growth stages and plant parts than wheat which had a greater stomatal conductance than barley. Seed size had a variable effect on WUE and did not affected $\Delta$ values. Water use efficiency was not affected by N rate at stem elongation in wheat and barley whereas WUE was increased 2 and 7%, respectively, in wheat and barley at anthesis with increasing N from 10 to 32 g m$^{-2}$ . High N plants had about 2.5% lower $\Delta$ values regardless of growth stages than low N plants across species and seed sizes. Carbon isotope discrimination was negatively correlated with WUE at anthesis but not at stem elongation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.14
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• pp.165-172
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• 1973

The sterile phenomenon is frequently found in the inter-species hybrids of ginseng as in other plants. It is known that among the hybrids between Panax Ginseng (PG) and Panax Quinquefolium (PQ), and between Panax Ginseng and Paxax Japonicus (PI), PG${\times}$PI is fertile only very rarely, while PG ${\times}$ PQ is always sterile. Therefore, in order to clarify the relationship between this sterility phenomenon and the metabolism of free amino acids, the changes of free amino acids through the formation of the flower organs and seeds of two hybrids, PG ${\times}$ PQ and PG ${\times}$ PI were investigated by thin layer chromatography. The results are summarized as follows: 1. Distinct differences in the quantity and number of free amino acids were recognized between PG ${\times}$ PQ, PG ${\times}$ PI and their parent plants. From the hybrid PG ${\times}$ PQ, 19 kinds of ninhyrin sensitive substances were detected in all. They were (1) 17 amino acids: alanine, valine, leucine, phenylalanine, proline, hydroxy-proline, serine, threonine, tyrosine, aspartic acid, glutamic acid, lysine, arginine, ${\gamma}$-amino butyric acid, ${\beta}$-alanine, cysteic acid and tryptophan, and (2) two amides: asparagine and glutamine. From the hybrid PG ${\times}$ PI, in addition to the above 19 substances, methionine and one unknown substance were detected. 2. Generally, alanine, as partie acid, glutamic acid, cysteic acid and asparagine were detected in large amounts in the two hybrids as in PG, PG and PJ but it was a noticeable fact concerning these two hybrids that the largest quantity of asparagine was found at microspore satge and pollen mature stage. 3. The decrease of cysteic acid in the two hybrids at the red ripened stage was the same as in PQ and PJ but opposite to the change in PG. The detection of methionine in PG ${\times}$ PJ was worthy of notice. 4. The change of proline was conspicuously different from that in their parent plants. It was detected as a trace of color at the micros pore stage while asparagine was detected in the greatest amount at that time. It is well known that the quantity of proline is closely related to the sterility of plant. This fact was also found true in the formation of ginseng seeds. It was reported as well that asparagine accumulated when proline decreased. 5. The deficiency of proline seemed to be closely related with the sterility of hybrids and with the degradation of pollen in anther. 6. The difference in the changes of free amino acids between the selfed lines of PG, PQ and PJ, and their hybrids seemed to be caused by the transformation of gene-action system by hybridization. On these phenomena along with proline metabolim and its physiological role in seed formation further studies are required.

• Korean Journal of Weed Science
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• v.30 no.3
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• pp.282-290
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• 2010

The objective of this research was to discover the best method for weed management in environment-friendly rice paddy fields through the study on the effect of weed control and injury levels of rice plants as affected by size, input time, and input amount of golden apple snail (GAS). The efficacy of weed control as affected by GAS when applied at 5, 10, and 15 days after transplanting (DAT) was 98, 89, and 58%, respectively. The efficacy of weed control had declined as late the input time of GAS. On the other hand, the efficacy of weed control as affected by rice bran followed by GAS treatment was higher than by GAS treatment alone. Weed species such as Persicaria hydropiper, Echinochloa crus-galli, Scirpus juncoides, and Monochoria vaginalis were not completely controlled by GAS when applied late. Input amount and time of adult GAS (70 days after hatching) for effective weed control were 3 kg $10a^{-1}$ at 5 DAT, 6-7 kg $10a^{-1}$ at 10 DAT, and 7 kg $10a^{-1}$ at 15 DAT. Input time and amount of young GAS (35 days after hatching) for effective weed control were 0 day after harrow (DAH) and 1 kg $10a^{-1}$, respectively. The young GAS when applied 0 DAH at 1 kg $10a^{-1}$ provided 100% control of P. hydropiper, E. crus-galli, S. juncoides, M. vaginalis, Ludwigia prostrata, Eleocharis kuroguwai, Sagittaria trifolia and Cyperus difformis. The rice foliar injury caused by adult (3 kg $10a^{-1}$) and young (1 kg $10a^{-1}$) GAS were 5-7% and 1% respectively. There was no significant difference in rice injury by size and input amount of GAS on plant height and number of tiller. These data indicate that the young GAS when applied 1 kg $10a^{-1}$ at 0 day after harrow was the best method for weed management in environment-friendly rice paddy fields.