• 한국미생물·생명공학회지
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• 제23권3호
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• pp.316-321
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• 1995

Some properties of $\beta$-1, 3-glucan synthase system in Saccharamyces cerevisiae were investigated. By extraction with detergent and salt, the membrane preparations could be dissociated into two components, one soluble, the other still membrane bound. Both components, in addition to GTP, were necessary for the activity of $\beta$-1, 3-glucan synthase like other fungi. The protective effect of guanosine nucleotides on the soluble factor pointed to the possibility that this fraction contained a GTP-binding protein. Addition of increasing amounts of soluble factor to a constant amount of insoluble catalytic factor, vice versa, gave rise to a saturation curve. These results, including different types of evidence, indicate that the soluble factor and the catalytic factor form a complex.

• 한국산학기술학회논문지
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• 제12권2호
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• pp.1020-1026
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• 2011

천안시 대기 입자 중 수용성 이온성분의 특성을 분석한 결과, 양이온은 $Na^+$과 $NH_4^+$이, 음이온은 $NO_3^-$와 $SO_4^{2-}$이 조대입자와 미세입자에서 모두 주요한 이온성분으로 조사되었다. 분석된 수용성 이온성분의 농도는 미세입자가 조대입자보다 높은 농도로 나타났으며, 겨울과 봄철에 높은 농도를, 여름철에 가장 낮은 농도를 나타냈다. 총 수용성 이온 성분은 전체 입자의 질량 중 조대입자에서는 계절별로 봄 24.4%, 여름 33.2%, 가을 40.7%, 겨울 39.6%를, 미세입자에서는 각각 43.0%, 59.7%, 55.4%, 53.2%를 차지하는 것으로 분석되어, 조대입자 보다는 미세입자에서 차지하는 비율이 컸으며, 다른 계절보다는 봄철에 낮은 비율을 나타냈다. 이들 수용성 이온성분의 요인분석을 수행한 결과 조대입자는 2개의 요인이, 미세입자는 4개의 요인이 추출되었다. 이로부터 조대입자의 경우 자연적 배출원으로 부터의 기여가 70.03%로, 미세입자의 경우 인위적 배출원으로 부터의 기여가 66.01%로 추정되었다.

• Journal of Microbiology and Biotechnology
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• 제16권4호
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• pp.576-583
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• 2006

The alkali-soluble glucan of the yeast cell wall contains $\beta-(1,3)-$ and (1,6)-D-linkages and is known to systemically enhance the immune system. In the previous study [6], in order to isolate cell wall mutants, a wild-type strain was mutagenized by exposure to ultraviolet light, and the mutants were then selected via treatment with laminarinase $(endo-\beta-(1,3)-D-glucanase)$. The mass of alkali- and water-soluble glucans produced by the mutant was measured to be 33.8 mg/g of the dry mass of the yeast cell. Our results showed that the mutants generated the amount of alkali-soluble glucan 10-fold higher than that generated by the wild-type. Structural analysis showed that the alkali-soluble glucan from the mutants was associated with a higher degree of $\beta-(1,6)-D-linkage$ than was observed in conjunction with the wild-type. Yeast cell wall $\beta-glucan$ was shown to interact with macrophages via receptors, thereby inducing the release of tumor necrosis factor alpha $(TNF-\alpha)$ and nitric oxide. Alkali-soluble $\beta-glucans$, both from water-soluble and water-insoluble glucan, exhibited a higher degree of macrophage activity with regard to both the secretion of tumor necrosis factor alpha $(TNF-\alpha)$ and nitric oxide and direct phagocytosis, than did the positive control ($1{\mu}g$ of lipopolysaccharide).

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 하계학술대회 논문집 C
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• pp.1785-1787
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• 2000

This paper present the result of the investigation, the electric properties of insulators due to non-soluble contamination. In general, the humidity and the amount of soluble salts such as NaCl, $MgCl_2$ are the most dominant factor. Though the non-soluble do not distribute on conductivity of contaminant layer, that has the hygroscopic property. For this study, we make a mini-fog chamber with transparent acryl and the kaoline was used for non-soluble contaminant. The kaoline was applied with sprayer to get the specific ESDD and NSDD value, then the specimen was dried and installed horizontally. And to measure the leakage current a DAS system was developed with LabView. With the result, we could know the influence of non-soluble content and the relationship between NSDD and ESDD.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2000년도 하계학술대회 논문집
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• pp.691-695
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• 2000

This paper present the result of the investigation, the electric properties of insulators due to non-soluble contamination. In general, the humidity and the amount of soluble salts such as NaCl, MgCl$_2$are the most dominant factor. Though the non-soluble do not distribute on conductivity of contaminant layer, that has the hygroscopic property. For this study, we make a mini-fog chamber with transparent acryl and the kaoline was used for non-soluble contaminant. The kaoline was applied with sprayer to get the specific ESDD and NSDD value, then the specimen was dried and installed horizontally. And to measure the leakage current a DAS system was developed with LabView. With the result, we could know the influence of non-soluble content and the relationship between NSDD and ESDD.

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1267-1272
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• 2005

The effects of coexpression of GroEL/ES and DnaK/DnaJ/GrpE chaperones on the productivity of the soluble form of human granulocyte colony stimulating factor (hG-CSF) in E. coli were examined. Recombinant hG-CSF protein was coexpressed with DnaK/DnaJ/GrpE or GroEL/ES chaperones under the control of the araB or Pzt-1 promoter, respectively. The optimal concentration of L-arabinose for the expression of DnaK/DnaJ/GrpE was found to be 1 mg/ml. When L-arabinose was added at $OD_{600}$=0.2 (early-exponential phase), soluble hG-CSF production was greatly increased. In addition, it was observed that the DnaK/DnaJ/GrpE and GroEL/ES chaperones had no synergistic effects on preventing aggregation of hG-CSF protein. Consequently, by coexpression of the DnaK/DnaJ/GrpE chaperone, the signal intensity of the hG-CSF protein band in the soluble fraction of cell lysate was increased from $3.5\%\;to\;13.9\%$, and Western blot analysis also revealed about a 4-5-fold increase of production of soluble hG-CSF over the non-induction case of DnaK/DnaJ/GrpE.

• 한국환경과학회지
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• 제25권5호
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• pp.715-726
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• 2016

This study investigates the characteristics of metallic and ionic elements concentration, concentration according to transport path, and factor analysis in $PM_{10}$ at Guducsan in Busan in the springtime of 2015. $PM_{10}$ concentration in Guducsan and Gwaebeopdong were $59.5{\pm}9.04{\mu}g/m^3$ and $87.5{\pm}20.2{\mu}g/m^3$, respectively. Contribution rate of water-soluble ions and secondary ion in $PM_{10}$ concentration in Guducsan were 37.0% and 27.8% respectively. [$NO_3{^-}/SO{_4}^{2-}$] ratio and contribution rate of sea salt of $PM_{10}$ in Guducsan and Gwaebeopdong were 0.91 and 1.12, 7.0% and 5.3%, respectively. The results of the backward trajectory analysis indicates that $PM_{10}$ concentration, total inorganic water-soluble ions and total secondary ions were high when the air parcels moved from Sandong region in China than non-Sandong and northen China to Busan area. The results of the factor analysis at Guducsan indicates that factor 1 was anthropogenic source effects such as automobile emissions and industrial combustion processes, factor 2 was marine sources such as sea salts from sea, and factor 3 was soil component sources.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2007년도 춘계학술대회 논문집
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• pp.149-154
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• 2007

The zinc prosphate film treatments used to lubricating treatment of mostly cold forging processes. But there are several problems happened to lubricating treatment process such as happening harmful environment on person, complex lubrication processing occurring in energy and time consumption, eco-destructive and chemical by-product generation, the needs of waste disposal etc. As a result, a water-soluble lubricant was developed to replace the perfect or some of the zinc prosphate film in the world. In order to solve these problems, this study evaluated the performance of the typical water-soluble. In this study, for these requirement inquiry of two part. First, about possibility of replace zinc phosphate lubricant, quantitatively evaluation developed of water-soluble lubricant for cold forging vs zinc phosphate lubricant. Second, About optimization of coating Process use to equipment with practicable automatic coating Process. The performance evaluation of these lubricants was conducted using the double cup extrusion test and spike forging test. With the use of the commercial FE code DEFORM, friction factor calibration curves, i.e. cup height ratio vs. punch stroke and spike height vs. punch stroke, were established for different friction factor values. By matching the cup height ratio and the punch stroke and spike height vs. punch stroke from experiment to that obtained from FE simulations, the friction factor of the lubricants was determined. Survey of comparative analysis use to SEM that sprayed lubricant surface structure of grain shape and characteristic of lubricant performance based on grain shape and deformed lubricant surface expansion. As a result, developed lubricant were found to perform comparable to or better than zinc phosphate. And thought this result, innovatively cope with generated problem of existing lubrication process.

• 한국임상수의학회지
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• 제31권3호
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• pp.170-174
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• 2014

본 연구는 개 소장점막하 조직을 겔화시킨 것과 일반적으로 사용하는 소 트롬빈, 그리고 이 두 가지 요소를 혼합한 것을 혈소판풍부혈장의 활성화 인자로 적용하여, 혈소판에서 분비되는 성장인자를 7일간 in vitro 실험을 실시하였다. 또한 전자현미경으로 각 그룹 별 샘플의 표면을 관찰하여, 측정한 성장인자와의 상관관계를 연구하였다. 그 결과, 그룹별로 유의적인 차이는 존재하지 않았지만, 성장인자인 (TGF)-${\beta}1$이 가장 많이 분비된 그룹은 혈소판풍부혈장을 개 소장점막하조직 겔로 활성화시켰을 때였다. 본 연구 결과를 통해, 혈소판풍부혈장을 활성화하기 위한 요소로 개소장점막하조직 겔은 소의 트롬빈을 대체할 만한 인자로 여겨지며, 추후 임상에서 다양하게 적용할 수 있을 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권12호
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• pp.1822-1829
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• 2003

Porcine colostrum and milk were separated into the acid-soluble and casein fractions by acidification followed by centrifuge. The acid-soluble fraction of porcine colostrum was further separated by liquid chromatography and anisotropic membrane filtration. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and their components was determined by incubating bovine trypsin or chymotrypsin in a medium containing their corresponding substrates with or without addition of various amounts of porcine colostrum, porcine milk or their components. The inhibition of insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) degradation in pig small intestinal contents by porcine colostrum was measured by incubating iodinated IGF-I or EGF with the intestinal contents with or without addition of porcine colostrum. Degradation of labeled IGF-I or EGF was determined by monitoring the generation of radioactivity soluble in 30% trichloroacetic acid (TCA). The results showed that porcine colostrum had high levels of trypsin and chymotrypsin inhibitory activity and increased the stability of IGF-I and EGF in pig intestinal contents. The inhibitory activity declined rapidly during lactation. It was also found that trypsin and chymotrypsin inhibitory activity and the inhibition on IGF-I and EGF degradation in the acid-soluble fraction were higher than that in the casein fraction. Heat-resistance study indicated that trypsin inhibitors in porcine colostrum survived heat treatments of $100^{\circ}C$ water bath for up to 10 min, but exposure to boiling water bath for 30 min significantly decreased the inhibitory activity. Compared with the trypsin inhibitors, the chymotrypsin inhibitors were more heatsensitive. Separation of the acid-soluble fraction of porcine colostrum by liquid chromatography and anisotropic membrane filtration revealed that the trypsin and chymotrypsin inhibitory capacity was mainly due to a group of small proteins with molecular weight of 10,000-50,000. In conclusion, the present study confirmed the existence of high levels of protease inhibitors in porcine colostrum, and the inhibition of porcine colostrum on degradation of milk-borne growth factors in the pig small intestinal tract was demonstrated for the first time.