• Wind and Structures
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• 제2권3호
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• pp.133-150
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• 1999

Significant costs to the public and private sectors due to recent extreme wind events have motivated the need for systematic post-hurricane damage data collection and analysis. Current post disaster data are collected by many different interested groups such as government agencies, voluntary disaster relief agencies, representatives of media companies, academicians and companies in the private sector. Each group has an interest in a particular type of data. However, members of each group collect data using different techniques. This disparity in data is not conducive to quantifying damage data and, therefore, inhibits the statistical and spatial description of damage and comparisons of damage among different extreme wind events. The data collection does not allow comparisons of data or results of analyses within a group and also prohibits comparison of damage data and information among different groups. Typically, analyses of data from a given event lead to different conclusion depending upon the definition of damage used by individual investigators and the type of data collected making it difficult for members of groups to compare the results of their analyses with a common language and basis. A formal method of data collection and analysis-within any single group-would allow comparisons to be made among different individuals, hazardous events and eventually among different groups, thus facilitating the management and reduction of damage due to future disaster. This research introduces a definition of damage to single family dwellings, and a common method of data collection and analysis suited for groups interested in regional characterization of damage. The current state-of-data is presented and a method for data collection is recommended based on these existing data collection methods. A fixed-scale damage index is proposed to consider the damage to a dwelling's feature. Finally, the damage index is applied to three dwellings damaged by Hurricane Iniki (1992). The damage index reflects the reduced functionality of a structure as a single family detached dwelling and provides a means to evaluate regional damage due to a single event or to compare damage due to events of different severity. Evaluation of the damage index and the data available support recommendation for future data collection efforts.

• 치과방사선
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• 제19권1호
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• pp.39-48
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• 1989

Six to eight-month-old female albino rats were used as experimental animals. As an irradiation equipment, a Co-60 was used. The experimental animals were divided to; 6 of the control group, 12 of the 500cGy single irradiation group, 12 of the 1000cGy fractionated irradiation group, and 12 of the 1500cGy fractionated irradiation group. From the first week to the forth, 3 rats were picked from each group every week to be sacrificed and fixed with formalin. Those rats were observed by means of H-E stain after being taken radiograph and decalcified. The analysis of radiographic findings and light microscopic findings gives results as follows: 1. The delay of dental eruption rate was found in every group which underwent the irradiation experiment. Dentin niche, osteodentin, and dentin island were formed in the parts which were damaged by the irradiation. 2. The longer the observation period was, the more deposit of osteodentin and dentin island was formed. 3. In the single irradiation group, the damage effect was in proportion to the increase of radiation dose, whereas the damage was much less in the fractionated group receiving the same dose. 4. The 500cGy single irradiation group got temporary repairable damage, while the 1000cGy single irradiation group got considerable damage and showed much slower eruption rate than the 500cGy single irradiation group. The basal portion of the 1500cGy single irradiation group, whose growth was arrested, was destroyed. 5. The fractionated group were irradiated 500cGy everyweek. Repair was visible during the interval periods. The damage was accumulated as irradiation repeated, but degree of damage was lower than that of the 1000cGy and 1500cGy single irradiation group.

• 한국환경성돌연변이발암원학회지
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• 제17권2호
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• pp.71-77
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• 1997

The single cell gel electrophoressis(SCGE) assay, also known as the comet assay, is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakage in mammalian cells. The SCGE or comet assay is a promising test for the detection of DNA damage and repair in individnal cells. It has widespread potential applications in DNA damage and repair studies, genotoxicity testing and biomonitoring. In this microgel electrophoresis technique, cells are embedded in agarose gel on microscope slides, iysed and electrophoresed under alkaline conditions. Cells with increased DNA damage display increased migration of DNA from the nucleus towards the anode. The length of DNA migration indicates the amount of DNA breakage in the cell. The comet assay is also capable of identifying apoptotic cells which contain highly fragmented DNA. Here we review the development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA and the potential applications of the technique.

• Environmental Analysis Health and Toxicology
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• 제16권1호
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• pp.17-20
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• 2001

The alkaline comet assay, employing a single-cell gel electrophoresis(SCGE), is a rapid, simple and sensitive technique for visualizing and measuring DNA damage leading to strand breakage in individual mammalian cells. The protecting effect of pretreatment with vitamin C and cysteine on the DNA damage of gamma ray was investigated in mice splenic lymphocytes. Vitamin C and cysteine were administered orally for five consecutive days before irradiation. Four week old ICR male mice were irradiated wish 3.5Gy of γ-radiation and were sacrificed 3 days later. Spleens were taken for DNA damage examination by Comet assay and the tail moments of DNA single -strand breaks in tole splenic lymphocytes were evaluated. The results show that pretreatment with vitamin C and cysteine were effective in protecting against DNA damage by gamma ray. Administration of antioxidants like vitamin C and cysteine to mice before irradiation was effective in reducing the tail moment of splenic lymphocytes DNA.

• 한국수산과학회지
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• 제36권4호
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• pp.346-351
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• 2003

Single-cell gel electrophoresis (comet assay) was used to detect DNA single strand break in blood cells from several marine fish species. Three fish species were collected from Georgia coastal area. Mummichog, Fundulus heteroclitus showed higher DNA damage than sea bass, Lateolabrax japonicus and trout, Oncorhynchus masou masou under the same experimental conditions. Mummichogs had more alkaline-labile sites on their DNA than other fish species. The comet assay with mummichog blood cells at pH 12.5 showed a dose-response curve with the increasing concentrations of hydrogen peroxide. While the isolated leucocytes showed no increase of DNA damage after in vitro exposure to 2-methyl-1,4-naphthoquinone (MNQ), erythrocytes showed dose-dependent DNA damage. These results indicate that the comet assay can be applied successfully as a bioassay using erythrocyte for environmental monitoring.

• Structural Engineering and Mechanics
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• 제51권6호
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• pp.1037-1046
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• 2014

Most existing damage detection methods based on curvature should investigate the indicator value changes between the intact and damaged state. However, the 'footprint' in the intact state is usually not available for most actual beams. Therefore, a new damage localization indicator called Difference of Nearby Difference Curvature Indicator (DNDCI) was introduced for single-span beams. This indicator does not require prior information of the intact beam and is sensitive to the damage occurs on or nearby the measuring points. Practical and detailed application method of DNDCI has been proposed. Besides the idealized simply supported beams, it was validated by example that DNDCI is also applicable to the actual single-span beams supported by bearings.

• 한국해양공학회지
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• 제23권6호
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• pp.124-130
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• 2009

This study presents a finite element simulation of elastic waves for detecting anti-symmetric damages in an adhesively-bonded single lap joint. Plane strain elements were used for modeling adherents (aluminum) and adhesives (epoxy). Three types of damage were introduced: thickness reduction, elasticity deterioration, and voids in the adhesive layers, and two excitation and reception arrangements (ER1 and ER2) were used to investigate the detectability of the damage. The simulation showed that symmetrically located damage, such as a thickness reduction, can be detected by one excitation and one reception arrangement (ER1) and anti-symmetric damages, such as elasticity deterioration and voids, can be detected by modified two-point elastic wave excitation (ER2). Compared with the ER1 arrangement, the ER2 arrangement does not require a baseline signal for damage detection; hence, an efficient method of anti-symmetric damage detection in an adhesively-bonded single lap joint is proposed.

• 약학회지
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• 제54권1호
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• pp.32-38
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• 2010

The aim of this study was to evaluate the effect of galangin towards hydrogen peroxide-induced DNA damage. The calf thymus DNA and Chinese Hamster Lung (CHL) cells were used to measure 8-hydroxy-2'-deoxyguanosine(8-OH2'dG) as an indicator of DNA oxidative damage using high performance liquid chromatography with electrochemical detection. Hydrogen peroxide in the presence of Fe(II) ion induced the formation of 8-OH2'dG in both calf thymus DNA and CHL cells. The DNA damage effects were enhanced by increasing the concentration of Fe(II) ion and inhibited by galangin. In the single cell gel electrophoresis (Comet assay), galangin and dl-a-tocopherol showed an inhibitory effect in CHL on hydrogen peroxide induced DNA single strand breaks. Galangin showed more potent activity than dl-$\alpha$-tocopherol under our experimental conditions. These results indicate that galangin can modify the action mechanisms of the oxidative DNA damage and may act as chemopreventive agents against oxidative stress.

• Environmental Analysis Health and Toxicology
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• 제21권2호
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• pp.127-138
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• 2006

In urban areas, diesel exhaust particles (DEP) are probably a major component of particulate matters, especially in Korea where drive many diesel vehicles. The aim of this study was to investigate genotoxic effects of DEP using single ceil gel electrophoresis. In order to evaluate the mechanisms of DEP genotoxicity, the rat microsome mediated and DNA repair enzyme treated comet assays together with conventional comet assay were performed. Total diesel particles (DEPT) was collected without site fractionation from diesel engine bus and dichloromethane extract was obtained. The organic extract of DEPT revealed DNA damage itself in NIH/3T3 cells. The level of DNA breaks plus oxidative DNA lesions and microsome mediated DNA damage was assessed by modified single cell gel eletrophoresis. DEPT was able to induce oxidative DNA damage as well as microsome mediated DNA damage. Vitamin C as an model antioxidant reduced DNA damage in endonuclase III treated comet assay. One of flavonoid, galangin as a CYP1A1 inhibitor. reduced DNA damage in the presence of S-9 mixture. $DEP_T$ is the sources of oxidative stress, but antioxidants can significantly reduce oxidative DNA dmage. And $DEP_T$ may contain indirect mutagens which can be inhibited by CYP1A1 inhibitors. The ethanol extracts of the mixed vegetables (BV) or the mixed fruits (BF) were evaluated for their in vitro antigenotoxic effects. BV and BF showed potent Inhibitory effects against DEPT induced DNA damage with oxidative DNA lesions and in the prescence of S-9 mixture. These results indicate that BV and BF could prevent cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P4501A1 in cell culture.

• Structural Engineering and Mechanics
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• 제84권2호
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• pp.181-206
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• 2022

This paper presents experimental modal analysis and static load testing results to validate the accuracy of dynamic parameters-based damage locating indices in RC structures. The study investigates the accuracy of different dynamic-based damage locating indices compared to observed crack patterns from static load tests and how different damage levels and scenarios impact them. The damage locating indices based on mode shape curvature and mode shape fourth derivate in their original forms were found to show anomalies along the beam length and at the supports. The modified forms of these indices show higher sensitivity in locating single and multi-cracks at different damage scenarios. The proposed stiffness reduction index shows good sensitivity in detecting single and multi-cracks. The proposed anomalies elimination procedure helps to remove the anomalies along the beam length. Also, the adoption of the proposed weighting method averaging procedure and normalization procedure help to draw the overall crack pattern based on the adopted set of modes.