• 정보처리학회논문지C
• /
• 제14C권7호
• /
• pp.559-570
• /
• 2007

본 논문에서는 NSIS(Next Step in Signaling) 시그널링 프로토콜을 기반으로 VPN(Virtual Private Network) QoS(Quality of Service) 서비스 모델인 Hose 모델 자원 준비(Resource Provisioning) 메커니즘에 따라 자원을 예약하고 관리하는 Hose-NSIS-QoSM을 제안한다. 구체적으로 Hose-NSIS-QoSM을 위한 NSIS 메시지 객체(Objects), QoS NSLP(NSIS Signaling Layer Protocol)/NTLP(NSIS Transport Layer Protocol)/RMF(Resource Management Function) 상태 테이블, NSIS 노드에서의 메시지 처리 과정을 정의하였다. 또한, MPLS 네트워크에서의 VPN의 Hose 기반 QoS 서비스 모델을 지원하기 위해 확장된 RSVP-TE(Resource Reservation Protocol-Traffic Engineering)를 이용하는 시그널링 방안인 Hose-RSVP-TE-QoSM과 본 논문에서 제안하는 방안인 Hose-NSIS-QoSM를 비교하였다.

• 대한한의학방제학회지
• /
• 제31권1호
• /
• pp.41-51
• /
• 2023

Objectives : Raphani Semen (Raphanus sativus L.) is known for the various beneficial effects in Korean medicine. This study aimed to investigate the effect of Raphani Semen extract (RSE) against arachidonic acid (AA)+iron-induced oxidative stress in cells. Methods : Ingredients, their target information, oxidative stress liver injury-related proteins was obtained from various network pharmacology databases and software. A hypergeometric test and enrichment analysis were conducted to evaluate associations between protein targets of RSE. The cell viability was assessed by MTT assay, and immunoblot analysis was used to confirm the molecular mechanisms. Results : A compound-target network of RSE was constructed, which consisted of 336 edges between 18 ingredients and 123 protein targets. PI3K-Akt signaling pathway, ErbB signaling pathway, HIF-1 signaling pathway, PPAR signaling pathway, and AMPK signaling pathway was significantly associated with protein targets of RSE. RSE protected HepG2 cells against AA+iron-induced oxidative stress as mediated with AMPK signaling. Conclusion : RSE was found to protect the cells against oxidative stress via the AMPK signaling pathway.

• The Korean Journal of Physiology and Pharmacology
• /
• 제14권3호
• /
• pp.139-144
• /
• 2010

In this study, we evaluated the role of apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1) on the tumor necrosis factor-$\alpha$ (TNF-$\alpha$) induced cyclooxygenase-2 (COX-2) expression using A549 lung adenocarcinoma cells. TNF-$\alpha$ induced the expression of COX-2 in A549 cells, but did not induce BEAS-2B expression. The expression of COX-2 in A549 cells was TNF-$\alpha$ dose-dependent (5~100 ng/ml). TNF-$\alpha$-stimulated A549 cells evidenced increased Ref-1 expression in a dose-dependent manner. The adenoviral transfection of cells with AdRef-1 inhibited TNF-$\alpha$-induced COX-2 expression relative to that seen in the control cells ($Ad{\beta}gal$). Pretreatment with $10\;{\mu}M$ of SB203580 suppressed TNF-$\alpha$-induced COX-2 expression, thereby suggesting that p38 MAPK might be involved in COX-2 expression in A549 cells. The phosphorylation of p38 MAPK was increased significantly after 5 minutes of treatment with TNF-$\alpha$, reaching a maximum level at 10 min which persisted for up to 60 min. However, p38MAPK phosphorylation was markedly suppressed in the Ref-1-overexpressed A549 cells. Taken together, our results appear to indicate that Ref-1 negatively regulates COX-2 expression in response to cytokine stimulation via the inhibition of p38 MAPK phosphorylation. In the lung cancer cell lines, Ref-1 may be involved as an important negative regulator of inflammatory gene expression.

• Molecules and Cells
• /
• 제38권12호
• /
• pp.1064-1070
• /
• 2015

Translocator protein 18 kDa (TSPO) is a mitochondrial outer membrane protein and is abundantly expressed in a variety of organ and tissues. To date, the functional role of TSPO on vascular endothelial cell activation has yet to be fully elucidated. In the present study, the phorbol 12-myristate 13-acetate (PMA, 250 nM), an activator of protein kinase C (PKC), was used to induce vascular endothelial activation. Adenoviral TSPO overexpression (10-100 MOI) inhibited PMA-induced vascular cell adhesion molecule-1 (VCAM-1) and intracellular cell adhesion molecule-1 (ICAM-1) expression in a dose dependent manner. PMA-induced VCAM-1 expressions were inhibited by Mito-TEMPO ($0.1-0.5{\mu}m$), a specific mitochondrial antioxidants, and cyclosporin A ($1-5{\mu}m$), a mitochondrial permeability transition pore inhibitor, implying on an important role of mitochondrial reactive oxygen species (ROS) on the endothelial activation. Moreover, adenoviral TSPO overexpression inhibited mitochondrial ROS production and manganese superoxide dismutase expression. On contrasts, gene silencing of TSPO with siRNA increased PMA-induced VCAM-1 expression and mitochondrial ROS production. Midazolam ($1-50{\mu}m$), TSPO ligands, inhibited PMA-induced VCAM-1 and mitochondrial ROS production in endothelial cells. These results suggest that mitochondrial TSPO can inhibit PMA-induced endothelial inflammation via suppression of VCAM-1 and mitochondrial ROS production in endothelial cells.

• International journal of advanced smart convergence
• /
• 제1권2호
• /
• pp.1-11
• /
• 2012

IP-based Wireless Sensor Networks (IP-WSNs) are gaining importance for their broad range of applications in health care, home automation, environmental monitoring, industrial control, vehicle telematics, and agricultural monitoring. In all these applications, a fundamental issue is the mobility in the sensor network, particularly with regards to energy efficiency. Because of the energy inefficiency of network-based mobility management protocols, they can be supported via IP-WSNs. In this paper, we propose a network-based mobility-supported IP-WSN protocol called mSFP, or the mSFP: "Multicasting-supported Inter-Domain Mobility Management Scheme in Sensor-based Fast Proxy Mobile IPv6 Networks". Based on [8,20], we present its network architecture and evaluate its performance by considering the signaling and mobility cost. Our analysis shows that the proposed scheme reduces the signaling cost, total cost, and mobility cost. With respect to the number of IP-WSN nodes, the proposed scheme reduces the signaling cost by 7% and the total cost by 3%. With respect to the number of hops, the proposed scheme reduces the signaling cost by 6.9%, the total cost by 2.5%, and the mobility cost by 1.5%. With respect to the number of IP-WSN nodes, the proposed scheme reduces the mobility cost by 1.6%.

• 한국정보통신학회:학술대회논문집
• /
• 한국해양정보통신학회 2001년도 추계종합학술대회
• /
• pp.93-96
• /
• 2001

Networks의 PSTN/IP망의 연동을 위한 신호연동 방안을 비교 분석하였으며, PSTN/IP망 구성 및 음성 호 설정을 위한 신호 연동 방안으로 SS7 신호방식 연동 방법에 대해 살펴보았다. 그리고 PSTN/IP 망간 지능망서비스 연동을 위한 신호방식 연동 방안으로, IP 망에서 PSTN SSP로 지능망서 비스 호 루팅 방법, IP망에서 PSTN SCP로 INAP/SS7 액세스 방법의 장단점을 비교 분석하였고, 이를 구현하기 위한 IETF SIGTRAN의 프로토콜 스택 구조를 살펴보았다.

• The Plant Pathology Journal
• /
• 제27권2호
• /
• pp.170-182
• /
• 2011

Plants possess multiple resistance mechanisms that protect themselves against pathogen attack. To identify unknown components of the defense machinery in Arabidopsis, gene-expression changes were monitored in Arabidopsis thaliana under 18 different biotic or abiotic conditions using a DNA microarray representing approximately 25% of all Arabidopsis thaliana genes (www.genevestigator.com). Seventeen genes which are early responsive to salicylic acid (SA) treatment as well as pathogen infection were selected and their T-DNA insertion mutants were obtained from SALK institute. To elucidate the role of each gene in defense response, bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 was inoculated onto individual T-DNA insertion mutants. Four mutants exhibited decreased resistance and five mutants displayed significantly enhanced resistance against Pst DC3000-infection as measured by change in symptom development as compared to wild-type plants. Among them, member of uridin diphosphate (UDP)-glycosyltransferase (UGT) was of particular interest, since a UGT mutant (At1g05680) showed enhanced resistance to Pst-infection in Arabidopsis. In systemic acquired resistance (SAR) assay, this mutant showed enhanced activation of SAR. Also, the enhanced SAR correlated with increased expression of defense-related gene, AtPR1. These results emphasize that the glycosylation of UGT74E2 is a part of the SA-mediated disease-resistance mechanism.

• 치위생과학회지
• /
• 제18권2호
• /
• pp.130-135
• /
• 2018

본 연구는 osteomac과 골세포가 골조직 개조과정에서 하는 역할을 조사하고자 수학적 모델링 방법을 사용하였다. 2013년에 발표된 Graham's model을 기반으로 하고 osteomac을 해당 네트워크에 추가하여 각 링크의 파라미터와 상미분방정식을 작성하였다. 컴퓨터 시뮬레이션 결과 osteomac과 골세포의 효율성이 일정 수치 이하인 경우 골조직 개조가 안정적이지 않고 감소하는 방향으로 이동하였다. 골세포의 효율성은 일정 수치 이상이 되어야 골조직 개조에도 골량이 유지되었으며 osteomac의 효율성의 수치가 증가함에 따라 골량도 함께 증가하는 것을 볼 수 있었다. Osteomac의 효율성이 골조직 개조와 골량 유지에 많은 영향을 미칠 수 있음을 제안한다.

• 전기학회논문지
• /
• 제56권11호
• /
• pp.1988-1991
• /
• 2007

This study investigates the performances of the signaling techniques including differential signals for the power-distribution network(PDN)s with and without the slit, using a rigorous evaluation method 'Modal Analysis', validated by being compared to the FDTD simulation up to 5 GHz.

• 한국정보통신학회:학술대회논문집
• /
• 한국해양정보통신학회 2005년도 추계종합학술대회
• /
• pp.275-279
• /
• 2005

In this paper, we analyses of a traffic for authentication signaling in third generation mobile sensor network. In universal mobile telecommunication system, authentication functions are utilized to identify and authentication a mobile station and validate the service request network services. The authenticating parties are the authentication the serving general packet radio service support node access the authentication center to obtain the authentication with the mobile station. In this paper, we propose that the automatic cost-effective solution size of the authentication vector array.