• 농업과학연구
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• 제36권2호
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• pp.159-165
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• 2009

본 연구는 현재까지 발표된 돼지의 genomic sequence 정보를 이용하여 PERV들의 정확한 삽입 위치를 파악하고 그들의 특성을 분석하고자 실시하였으며 총 2.7 Gb인 돼지 genome 염기서열 중 4.2%인 114 Mb의 염기서열에서 PERV sequence를 확인한 결과 총 8개의 PERV sequence를 확인할 수 있었다. 확인된 PERV sequence중 7개는 유전자내에 deletion이 확인되었으며 나머지 한 개의 PERV도 gag와 env 유전자에 stop codon이 확인되어 정상적인 PERV로 발현되지 않을 것으로 추정되었다. 본 연구는 돼지를 이용한 이종장기이식과 관련하여 PERV를 제어하기 위한 중요한 기초 연구 자료를 제공할 것으로 사료된다.

• BMB Reports
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• 제47권6호
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• pp.330-335
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• 2014

Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. In this study, RNA1 sequence of Flock house virus (FHV) was inserted into the TYMV genome to test whether TYMV can accommodate and express another viral entity. In the resulting construct, designated TY-FHV, the FHV RNA1 sequence was expressed as a TYMV subgenomic RNA. Northern analysis of the Nicotiana benthamiana leaves agroinfiltrated with the TY-FHV showed that both genomic and subgenomic FHV RNAs were abundantly produced. This indicates that the FHV RNA1 sequence was correctly expressed and translated to produce a functional FHV replicase. Although these FHV RNAs were not encapsidated, the FHV RNA having a TYMV CP sequence at the 3'-end was efficiently encapsidated. When an eGFP gene was inserted into the B2 ORF of the FHV sequence, a fusion protein of B2-eGFP was produced as expected.

• 한국멀티미디어학회논문지
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• 제15권11호
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• pp.1358-1368
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• 2012

RFID (Radio Frequency Identification) 태그 순차 추정 기법은 RFID 리더의 움직임을 이용하여 선형적으로 배열된 수동형 태그들의 물리적인 순서를 추정하는 것이다. 수동형 태그와 리더는 전자기 유도에 의한 미세 전파를 이용하여 통신하므로 수동형 태그의 스캔(scan)은 불안정하게 이루어진다. 본 논문에서는 순차 추정의 정확도를 높이기 위해 리더의 왕복 스캔을 이용하였다. 제안 기법은 먼저 리더의 움직임이 바뀌는 반환점을 찾아낸다. 그리고 이동 방향별로 매 읽기 주기에 읽혀지는 태그들의 집합을 이용하여 태그 전체의 순차를 추정한다. 실험을 통해 제안하는 기법이 다양한 환경에서 순차추정의 정확도를 높일 수 있음을 보였다.

• 전기전자학회논문지
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• 제1권1호
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• pp.83-92
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• 1997

대용량 스위칭 스루풋 요구를 만족시키면서 초고속 라인 속도를 처리해야하는 대용량. 초고속 ATM 스위칭 구조설계에는 다수경로 특성을 갖는 구조를 이용한다. 그러나 다수경로 특성을 갖는 스위칭 구조에서는 순서바뀜현상이 피할 수 없이 발생한다. 이 논문에서는 다수경로 스위칭 구조의 특성을 분석하여 순서 바뀜 가능성을 정량적으로 분석해볼 수 있는 분석모델을 제안한다. 그리고 이 모델을 이용하여 다수경로 스위치의 구조 파라메타들과 셀 순서바뀜현상과의 관계를 분석한다. 이 파라메타로는 다수경로의 수 (L), 입력 셀스트림의 특성, 스위치 크기(N), 및 트렁크를 구성하는 가상회선 수($V_{ch}$)등이며, 다수경로가 순서바뀜현상을 일으킬 때에 미치는 영향을 분석한다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.120-120
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• 1995

The total size of the pF AR1, a genomic clone of Frankia FaCI, was estimated to be about 44Kb by summation of the individual fragment length generated by single or double restriction enzymes. Southern hybridization analyses with Azotobacter vinelandii nif-genes as probes and partial sequencing analyses of the subclones revealed that organization of the nif-gene in the FaCI strain was nifV, H, D, K, E, N, X, W, B. The organization of the structural genes for nitrogenase is the same in this Frankia strain as it is in most other nitrogen-fixing prokaryotes but the positioning of the nifV-like gene relative to the nifHDK cluster differs. A consensus nif-promoter-like sequence, found at 5' of nifH, was not detected upstream of the niJV-like gene. nifV-like gene contained a ORF of 1206 NT encoding 401 amino acids. The nucleotide sequence and deduced amino acid sequence of the gene exhibit homology value of 65% and 41% with that from A vinelandii, respectively. The putative Shine-Dargamo sequences were present preceding nitK, nifH, D, K, and nifE, and in nitK gene putative start codon GTG was detected instead of A TG. The nucleotide and amino acid sequence of niIK of FaCI showed 82% and 76% homolgy with those of Frankia HFPCc 13, respectively. Amino acid sequence of niIK showed 69% and 61% homology with those of A vinelandii, Klebsiella pnewnoniae, respectively, while that of nifE 73% and 71%, respecti vely.i vely.

• 전력전자학회논문지
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• 제20권6호
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• pp.526-533
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• 2015

A new algorithm is proposed for the estimation of equivalent grid impedance at the point of common coupling of a grid-tie inverter output. The estimated impedance parameter can be used for the improvement of the performance and the stability of the distributed generation system. The estimation error is inevitable in the conventional estimation method because of the axis rotation due to PLL. In the conventional estimation error, the d-q voltage and current are used for the calculation of the impedance with active and reactive current injections. Conversely, in the proposed algorithm, the negative sequence current is injected, and then the negative sequence voltage is measured for the impedance estimation. As the positive and negative sequence current controller is independent and the PLL is based on the positive sequence component only, the estimation of the equivalent impedance can be achieved with high accuracy. Simulation and experimental results are compared to validate the proposed algorithm.

• 대한수학회논문집
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• 제39권2호
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• pp.437-460
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• 2024

In this paper we present the weighted shift operators having the property of moment infinite divisibility. We first review the monotone theory and conditional positive definiteness. Next, we study the infinite divisibility of sequences. A sequence of real numbers γ is said to be infinitely divisible if for any p > 0, the sequence γ^p = {γ^p_n}^∞_n=0 is positive definite. For sequences α = {α_n}^∞_n=0 of positive real numbers, we consider the weighted shift operators W_α. It is also known that W_α is moment infinitely divisible if and only if the sequences {γ_n}^∞_n=0 and {γ_n+1}^∞_n=0 of W_α are infinitely divisible. Here γ is the moment sequence associated with α. We use conditional positive definiteness to establish a new criterion for moment infinite divisibility of W_α, which only requires infinite divisibility of the sequence {γ_n}^∞_n=0. Finally, we consider some examples and properties of weighted shift operators having the property of (k, 0)-CPD; that is, the moment matrix M_γ(n, k) is CPD for any n ≥ 0.

• Archives of Plastic Surgery
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• 제35권3호
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• pp.349-353
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• 2008

Purpose: There are 3 well-known surgical procedures to treat Pierre Robin sequence: tongue-lip adhesion, distraction osteogenesis of mandible, and tracheostomy. The classical tongue-lip adhesion is an effective way to keep airway. The tongue, however, becomes quite non-mobile and appears dormant until the patient could control upper airway and the adhesion can be maintained for prolonged period. Most of all, this procedure does not provide the correction of the micrognathia. Distraction osteogenesis is a good technique to correct micrognathia and to prevent tracheostomies in patients with Pierre Robin sequence. But airway keeping procedure is needed during the distraction period. The purpose of this study is to determine the usefulness of temporary tongue-lip traction during the initial period of mandibular distraction in Pierre Robin sequence patients with severe airway problems requiring operative procedure. Methods: It was a prospective study of 2 Pierre Robin sequence patients aged between 4 months and 6 months requiring surgical procedure to correct recurrent and severe pulmonary complications. Two patients underwent distraction osteogenesis of mandible. During the operation, deep one tension suture was performed to tract the tongue and lip. When the patient gained control of upper airway at the initial period of distraction and micrognathia was corrected, the traction suture was removed. Results: All patients were followed up. No patients complained severe pulmonary complications and tracheostomy could be avoided. No patients had severe pulmonary complication. The pulmonary condition of patients was good. Conclusion: In severe Pierre Robin sequence case, temporary tongue-lip traction is a good assistant method in distraction osteogenesis because this method can avoid tracheostomy.

• Journal of Microbiology and Biotechnology
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• 제30권1호
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• pp.109-117
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• 2020

Cre recombinase is widely used to manipulate DNA sequences for both in vitro and in vivo research. Attachment of a trans-activator of transcription (TAT) sequence to Cre allows TAT-Cre to penetrate the cell membrane, and the addition of a nuclear localization signal (NLS) helps the enzyme to translocate into the nucleus. Since the yield of recombinant TAT-Cre is limited by formation of inclusion bodies, we hypothesized that the positively charged arginine-rich TAT sequence causes the inclusion body formation, whereas its neutralization by the addition of a negatively charged sequence improves solubility of the protein. To prove this, we neutralized the positively charged TAT sequence by proximally attaching a negatively charged poly-glutamate (E12) sequence. We found that the E12 tag improved the solubility and yield of E12-TAT-NLS-Cre (E12-TAT-Cre) compared with those of TAT-NLS-Cre (TAT-Cre) when expressed in E. coli. Furthermore, the growth of cells expressing E12-TAT-Cre was increased compared with that of the cells expressing TAT-Cre. Efficacy of the purified TAT-Cre was confirmed by a recombination test on a floxed plasmid in a cell-free system and 293 FT cells. Taken together, our results suggest that attachment of the E12 sequence to TAT-Cre improves its solubility during expression in E. coli (possibly by neutralizing the ionic-charge effects of the TAT sequence) and consequently increases the yield. This method can be applied to the production of transducible proteins for research and therapeutic purposes.

• Molecules and Cells
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• 제27권3호
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• pp.365-381
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• 2009

The chloroplast DNA sequences of Megaleranthis saniculifolia, an endemic and monotypic endangered plant species, were completed in this study (GenBank FJ597983). The genome is 159,924 bp in length. It harbors a pair of IR regions consisting of 26,608 bp each. The lengths of the LSC and SSC regions are 88,326 bp and 18,382 bp, respectively. The structural organizations, gene and intron contents, gene orders, AT contents, codon usages, and transcription units of the Megaleranthis chloroplast genome are similar to those of typical land plant cp DNAs. However, the detailed features of Megaleranthis chloroplast genomes are substantially different from that of Ranunculus, which belongs to the same family, the Ranunculaceae. First, the Megaleranthis cp DNA was 4,797 bp longer than that of Ranunculus due to an expanded IR region into the SSC region and duplicated sequence elements in several spacer regions of the Megaleranthis cp genome. Second, the chloroplast genomes of Megaleranthis and Ranunculus evidence 5.6% sequence divergence in the coding regions, 8.9% sequence divergence in the intron regions, and 18.7% sequence divergence in the intergenic spacer regions, respectively. In both the coding and noncoding regions, average nucleotide substitution rates differed markedly, depending on the genome position. Our data strongly implicate the positional effects of the evolutionary modes of chloroplast genes. The genes evidencing higher levels of base substitutions also have higher incidences of indel mutations and low Ka/Ks ratios. A total of 54 simple sequence repeat loci were identified from the Megaleranthis cp genome. The existence of rich cp SSR loci in the Megaleranthis cp genome provides a rare opportunity to study the population genetic structures of this endangered species. Our phylogenetic trees based on the two independent markers, the nuclear ITS and chloroplast MatK sequences, strongly support the inclusion of the Megaleranthis to the Trollius. Therefore, our molecular trees support Ohwi's original treatment of Megaleranthis saniculifolia to Trollius chosenensis Ohwi.