• Genomics & Informatics
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• v.5 no.4
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• pp.174-178
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• 2007

The earliest stages of mammalian embryogenesis are governed by the activity of maternally inherited transcripts and proteins. Cytoplasmic polyadenylation of selected maternal mRNA has been reported to be a major control mechanism of delayed translation during preimplantation embryogenesis in mice. The presence of cis-elements required for cytoplasmic polyadenylation (e.g., CPE) can serve as a useful tag in the screening of maternal genes partaking in key functions in the transcriptionally dormant egg and early embryo. However, due to its relative simplicity, UA-rich sequences satisfying the canonical rule of known CPE consensus sequences are often found in the 3'-UTR of maternal transcripts that do not actually undergo cytoplasmic polyadenylation. In this study, we developed a method to confirm the validity of candidate CPE sequences in a given gene by a multiplex comparison of 3'-UTR sequences between mammalian homologs. We found that genes undergoing cytoplasmic polyadenylation tend to create a conserved block around the CPE, while CPE-like sequences in the 3'-UTR of genes lacking cytoplasmic polyadenylation do not exhibit such conservation between species. Through this cross-species comparison, we also identified an alternative CPE in the 3'-UTR of tissue-type plasminogen activator (tPA), which is more likely to serve as a functional element. We suggest that verification of CPEs based on sequence conservation can provide a convenient tool for mass screening of factors governing the earliest processes of mammalian embryogenesis.

• BMB Reports
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• v.38 no.1
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• pp.49-57
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• 2005

Major Royal Jelly Protein cDNAs of Apis cerana (AcMRJP) were cloned and characterized. The open reading frames (ORFs) of the AcMRJP1 and AcMRJP2 genes were 1302 and 1392 nucleotides, encoding 433 and 463 amino acid residues, respectively. The sequence divergences between AcMRJP1 and AcMRJP2 and their corresponding protein families in A. mellifera were 0.0618 and 0.0934 at the nucleotide level and 0.0912 and 0.1438 at the protein level, respectively. Phylogenetic analysis supports the orthologous similarity between these proteins. The deduced amino acids indicated high essential amino acid contents of AcMRJP1 and AcMRJP2 (47.5 and 44.8%, respectively). The genomic organization of both AcMRJP1 and AcMRJP2 was determined. Both the AcMRJP1 (3663 bp) and AcMRJP2 (3963 bp) genes contained six exons and five introns, where all boundaries conformed to the GT/AG rule. AcMRJP1 and AcMRJP2 cDNAs were cloned into pET17b, and both the recombinant (r) AcMRJP1 (47.9 kDa) and rAcMRJP2 (51.7 kDa) were expressed in the insoluble form. Western blot analysis and N-terminal sequencing of the solubilized proteins revealed successful expression of rAcMRJP1 and rAcMRJP2 in vitro. The yields of the purified rAcMRJP1 and rAcMRJP2 were approximately 20 and 8mg protein per liter of the flask culture, respectively.

• Information Systems Review
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• v.10 no.2
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• pp.195-209
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• 2008

Customers' needs change every moment. Profitability of stores can't be increased anymore with an existing standardized chain store management. Accordingly, a personalized store management tool needs through prediction of customers' preference. In this study, we propose a recommending procedure using dynamic customers' preference by analyzing the transaction database. We utilize self-organizing map algorithm and association rule mining which are applied to cluster the chain stores and explore purchase sequence of customers. We demonstrate that the proposed methodology makes an effect on recommendation of products in the market which is characterized by a fast fashion and a short product life cycle.

• Journal of Pharmaceutical Investigation
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• v.35 no.4
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• pp.287-293
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• 2005

The purpose of the present study was to evaluate the bioequivalence of two glimepiride tablets, Amaryl tablet (Handok & Aventis Korea, reference drug) and Mepiril tablet (Myungmoon Pharm. Co., Ltd., Korea, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). After adding an internal standard (glibenclamide) to human plasma, plasma samples were extracted using 1mL of methyl tertiary butyl ether. Compounds extracted were analyzed by reverse-phase HPLC with multiple reaction monitoring (MRM) mode analyte detection. This method for determination glimepiride proved accurate and reproducible, with a limit of quantitation of 2 ng/mL in human plasma. Twenty-four healthy male Korean volunteers received each medicine at the glimepiride dose of 2 mg in a $2{\times}2$ crossover study. There was a one-week washout period between the doses. Plasma concentrations of glimepiride were monitored by a LC-MS/MS for over a period of 12 hr after the administration. $AUC_t$ (the area under the plasma concentration-time curve from time zero to 12 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (maximum plasma drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the plasma concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_t$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{max}$ ratio for Amaryl/Mepiril were log 0.9583-log 1.1357 and log 1.0570-log 1.2376, respectively. These values were within the acceptable bioequivalence intervals of log 0.80-log 1.25. Taken together, our study demonstrated the bioequivalence of Amaryl and Mepiril with respect to the rate and extent of absorption.

• Journal of Pharmaceutical Investigation
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• v.35 no.5
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• pp.355-360
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• 2005

The purpose of the present study was to evaluate the bioequivalence of two cetirizine HCl tablets, Zyrtec tablet (UCB Pharm. Co., Ltd. Korea, reference product) and Zyrix tablet (Kukje Pharm. Co., Ltd., Korea, test product), according to the guidelines of Korea Food and Drug Administration (KFDA). After adding an internal standard (diazepam), plasma samples were extracted using 1 mL of dichloromethane. Compounds extracted were analyzed by reverse-phase HPLC with ultra-violet detector. This method for determination cetirizine is proved accurate and reproducible with a limit of quantitation of 10 ng/mL in male plasma. Twenty-four healthy male Korean volunteers received each medicine at the cetirizine HCl dose of 10 mg in a $2{\times}2$ crossover study. There was a one-week wash out period between the doses. Plasma concentrations of cetirizine were monitored for over a period of 24 hr after the administration. AUC (the area under the plasma concentration-time curve) was calculated by the linear trapezoidal rule. $C_{max}$ (maximum plasma drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the plasma concentration-time data. Analysis of variance was carried out using logarithmically transformed AUC and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals for the log transformed data were acceptable range of log 0.8 to log 1.25 $(e.g.,\;log\;0.93-log\;1.08\;for\;AUC_{0-t},\;log\;0.91-log\;1.08\;for\;AUC_{0-{\infty}}\;and\;log\;1.01-log\;1.11\;for\;C_{max})$. The major parameters, AUC and $C_{max}$ met the criteria of KFDA for bioequivalence indicating that Zyrix tablet is bioequivalent to Zyrtec tablet.

• Journal of Microbiology and Biotechnology
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• v.25 no.12
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• pp.2082-2089
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• 2015

Avian beta-defensin 9 (AvBD9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. Owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, AvBD9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. Using the preferred codon of Pichia pastoris, the mature AvBD9 peptide was designed and synthesized, based on the sequence from GenBank. The P. pastoris constitutive expression vector pGHKα was used to construct a pGHKα-AvBD9 recombinant plasmid. Restriction enzyme digestion was performed using SacI and BglII to remove the ampicillin resistance gene, and the plasmid was electrotransformed into P. pastoris GS115. High-expression strains with G418 resistance were screened, and the culture supernatant was analyzed by Tricine-SDS-PAGE and western blot assay to identify target bands of about 6 kDa. A concentrate of the supernatant containing AvBD9 was used for determination of antimicrobial activity. The supernatant concentrate was effective against Escherichia coli, Salmonella paratyphi, Salmonella pullorum, Pseudomonas aeruginosa, Enterococcus faecalis, and Enterobacter cloacae. The fermentation product of P. pastoris carrying the recombinant AvBD9 plasmid was adjusted to 1.0 × 10⁸ CFU/ml and added to the drinking water of white feather broilers at different concentrations. The daily average weight gain and immune organ indices in broilers older than 7 days were significantly improved by the AvBD9 treatment.

• Journal of the Korean Institute of Telematics and Electronics
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• v.26 no.5
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• pp.137-144
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• 1989

A new algorithm is presented which efficiently reports minimum width/space violation in a geometric mask pattern. The proposed algorithm solves a sequence of range search problems by employing a plane sweep method. The algorithm runs in O(n log n) time, where n is the number of edges in a mask pattern. Since a lower bound in time conplexity for reporting all minimum width/space violations is ${\Omega}$ (n log n), this algorithm is theoretically optimal within a constant multiplicaive factor. It requires O($n^{0.5}$) space which is very efficient in practice. Moreover, this algorithm, we believe, is easy to implement and practically fast (116.7 seconds for a rectilinear region with 250000 vertices ar VAX 8650.)

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.40 no.6
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• pp.90-97
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• 2003

This paper proposes a speech recognition based on HMM(Hidden Markov Model) using FSVQ(First Section Vector Quantization) and fuzzy concept. In the proposed paper, we generate codebook of First Section, and then obtain multi-observation sequences by order of large propabilistic values based on fuzzy rule from the codebook of the first section. Thereafter, this observation sequences of first section from codebooks is trained and in case of recognition, a word that has the most highest probability of first section is selected as a recognized word by same concept. Train station names are selected as the target recognition vocabulary and LPC cepstrum coefficients are used as the feature parameters. Besides the speech recognition experiments of proposed method, we experiment the other methods under same conditions and data. Through the experiment results, it is proved that the proposed method based on HMM using FSVQ and fuzzy concept is superior to tile others in recognition rate.

• Fashion & Textile Research Journal
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• v.10 no.6
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• pp.1014-1022
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• 2008

This study focuses on practical appliance of color therapy to skin care. After visiting the notion of color therapy and skin care, this study considers the ideas and background theories for diagnosis of constitution and skin. Cosmic duals force five element theory developed in China was the first introduction; Ayurvedic medicine and seven chakra notion are examined. Cosmic duals force five element theory, an idea describing the composition and movement rule of the cosmic components, adopted five colors(white, yellow, red, blue, and black) for cure purposes. In future, mental effect of the color is examined in the latter part of this study. The practice of the color therapy assigns solarized cream treatment and photo therapy, simultaneously proposes the use of color therapy to the skin care. For the diagnosis of the constitution, cosmic duals force five element theory and ayurvedic medicine are followed in a sequence. Cosmic duals force five element theory introduces five colors. Constitution medicine considers four types, ayurvedic medicine adopts three types, and seven colors are mentioned as chakra colors. Such a variations lead to possible disagreement on constitution analysis and driven colors. This study adopts the above methodologies for selecting potential color therapy for skin care. Adopt of selected cases are purely a part of the proposal. Initially, examined theories serve as a pilot for selecting a representative hypothesis. Followed selection of constitution and matched color for the use of the skin care are the scope of this study. In summary, background methodologies are implemented for the calculation of color therapy.

• Proceedings of the KIEE Conference
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• 2003.11b
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• pp.295-298
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• 2003

In this paper, a novel maximum a posterion (MAP) estimation for the channel decoding of H.264 codes in the presence of transmission error is presented. Arithmetic codes with a forbidden symbol and trellis search techniques are employed in order to estimate the best transmitted. And, there has been growing interest of communication, the research about transmission of exact data is increasing. Unlike the case of voice transmission, noise has a fatal effect on the image transmission. The reason is that video coding standards have used the variable length coding. So, only one bit error affects the all video data compressed before resynchronization. For reasons of that, channel needs the channel codec, which is robust to channel error. But, usual channel decoder corrects the error only by channel error probability. So, designing source codec and channel codec, Instead of separating them, it is tried to combine them jointly. And many researches used the information of source redundancy In received data. But, these methods do not match to the video coding standards, because video ceding standards use not only one symbol but also many symbols in same data sequence. In this thesis, We try to design combined source-channel codec that is compatible with video coding standards. This MAP decoder is proposed by adding semantic structure and semantic constraint of video coding standards to the method using redundancy of the MAP decoders proposed previously. Then, We get the better performance than usual channel coder's.