• Asian-Australasian Journal of Animal Sciences
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• v.28 no.12
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• pp.1721-1728
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• 2015

Embryonic stem cells (ESCs) have been used as a powerful tool for research including gene manipulated animal models and the study of developmental gene regulation. Among the critical regulatory factors that maintain the pluripotency and self-renewal of undifferentiated ESCs, NANOG plays a very important role. Nevertheless, because pluripotency maintaining factors and specific markers for livestock ESCs have not yet been probed, few studies of the NANOG gene from domestic animals including bovine have been reported. Therefore, we chose mouse ESCs in order to understand and compare NANOG expression between bovine, human, and mouse during ESCs differentiation. We cloned a 600 bp (-420/+181) bovine NANOG 5'-flanking region, and tagged it with humanized recombinant green fluorescent protein (hrGFP) as a tracing reporter. Very high GFP expression for bovine NANOG promoter was observed in the mouse ESC line. GFP expression was monitored upon ESC differentiation and was gradually reduced along with differentiation toward neurons and adipocyte cells. Activity of bovine NANOG (-420/+181) promoter was compared with already known mouse and human NANOG promoters in mouse ESC and they were likely to show a similar pattern of regulation. In conclusion, bovine NANOG 5-flanking region functions in mouse ES cells and has characteristics similar to those of mouse and human. These results suggest that bovine gene function studied in mouse ES cells should be evaluated and extrapolated for application to characterization of bovine ES cells.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.665-680
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• 2016

Theoretically, it is said that economies of scope can be realized in the cropelivestock cycling organic farming. Thus, it is also used as the principle of organic farming. However, it is difficult to find the cases of the empirical analysis of it in Korea. In that sense, this study is meaningful in that it analyzed the agricultural data of case farms of obtaining the approval of both organic agricultural products and organic animal products and practicing cycling farming for 4 years and tested the hypothesis. This study measured economies of scope by using the actual measurement value and estimation value farming performance statistics for 4 years of case farms. This farmhouse conducted nutrient cycling in the farm like self-manufacturing and injecting organic agricultural byproduct and wild grass as organic livestock feed and fermenting organic livestock manure to organic compost to return it 100%. The results can be summarized as follows: According to the result of cycling farming of combining and producing organic agriculture and organic livestock, economies of scope were found to be realized in this case farmhouse. That is, although not strong, EOS>0, there were economies of scope. The measurement value appeared as 0.0722, 0.00378, 0.04667 and 0.13127 in 2012, 2013, 2014 and 2015, respectively. It was improved as time passes and the scale gets smaller. Therefore, in order to further improve economies of scope, there should be measures of reducing duplication costs between agriculture-livestock as low as possible and lowering the production cost of organic feed. That is, there is a need for the management strategy to adjust the import function and cost function according to the change in management paradigm and cropping system.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.6
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• pp.1433-1440
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• 2003

This research was performed to investigate protective effect of Sophorae subprostratae Radix and each fractions against ischemic damage using PC12 cells. To observe the protective effect of Sophorae subprostratae Radix on ischemia damage, vibility and changes in activities of Superoxide dismutase (SOD), Glutathione Peroxidase (GPx), Catalase and Production of Malondialdehyde (MDA) were observed after treating PC12 cells with Sophorae subprostratae Radix during ischemic insult. Groups were divided into five groups: no treated (Normal), hypoxia chamber for 48hrs followed by 6h at normoxic chamber (H/R), Sop horae subprostratae Radix total phase treated group with H/R (Total), Sophorae subprostratae Radix water phase treated group with H/R (Water), Sophorae subprostratae Radix BuOH phase treated group with H/R (BuOH), Sophorae subprostratae Radix alkaloid phase treated group with H/R (Alkaloid). The results showed that (1) in hypoxiajreperfusion model using PC12 cell, the Sophorae subprostratae Radix has the protective effect against ischemia in the dose of 0.2 ㎍/㎖, 2 ㎍/㎖ and 20 ㎍/㎖, (2) Sophorae subprostratae Radix increased the activities of glutathione peroxidase and catalase. (3) the activity of Superoxide Diamutase(SOD) increased by ischemic damage, which might represent the self protection. This study suggests that Sophorae subprostratae Radix has neuroprotective effect against neuronal damage following hypoxiajreperfusion cell culture model using PC12 cell and dose dependency effects. In conclusion, Sophorae subprostratae Radix has protective effects against ischemic oxidative damage at the early stage of ischemia.

• Research in Community and Public Health Nursing
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• v.11 no.1
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• pp.117-126
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• 2000

For basic data to develop health management program of female workers. this study was intended to characterize factors affecting health problems of women at workplace which has less than 50 workers by Cornell Medical Index(CMI). 195 items of CMI questionnaire were simplified to 57 items which were composed of 35 items concerning physical health problems and 22 items concerning mental health problems. The data was collected during 6 months from July 10 to December 30 and analyzed with frequency, percentage, t-test and ANOVA. The 107 female workers included in this study was characterized to be mainly twenties(64.5%), single(69.2%) and working at production position(89.7%). The most frequent physical problem was fatigability and the next, neurological system and cardiovascular system. Main mental complaints were inadquacy. anger and tension. When the health problems were reviewed according to its general characteristics, anger in young age, adaptiveness in single and respiratory complaints in self-recognized unhealthy group were prevalent. According to life styles. the prevalent complaints were musculoskeletal one in alcoholic group and cardiovascular one in group who are sleeping just for four to five hours. The appropriateness of each items of CMI should be evaluated to reveal the reasons why meaningful results could not come from many items of this questionnaire. The repeated study should be carried out to figure out the health problems and its related factors of female workers at small scale workplace. Also, exercise program and abstinence from drinking and smoking should be carried out for female worker's health.

• Journal of the Korean Society of Food Culture
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• v.12 no.1
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• pp.87-96
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• 1997

The purpose of this study is, to examine current foodservice management practices at free meal service organization for elderly people and, to evaluate the attitude of recipients about the service and their ecological background. 6 meal service center as well as randomly selected 120 recipients at Sungnam area were surveyed and interviewed and result were summarized as follow. The cost of each meal (lunch) was ranged from 1,300 won to 1,500 won and number of attendant at meal service were ranged from 50 to 200 persons. Meal time for lunch begins from 10:30 am to noon because greater portion of people (elderly) didn't take breakfast frequently. Most of the center adapted self-service system. Standard recipe was not developed and meal preparation was controlled under the experiences of volunteer’s. Recording system of, nutrition management, production control, storage and inventory control was not well adapted by most of the center. In order to measure the level of storage, sanitation etc., scorin system in survey was adapted in this study and result are as follow: The score of sanitation of kitchen was lower than dinning area and that of food storage was lowest score. It was suggested that not only financial but also systematical support on management by local government may be necessary to meet the goal of supply nutritionally balanced food at the center. The score given by the recipient on the satisfaction of meal service was rate as 4.8 at the 5-point maximum scale. Meeting friends and share social relationship was major reason (41.6% of the total) of visiting to the center. It is suggested that in order to meet the changes of the patterns of change of social and family structure, the service of the center should be extended in urban area and it is necessary to develop systematic management models for the center.

• Journal of Korean Public Health Nursing
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• v.14 no.1
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• pp.147-160
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• 2000

This study was carried out to investigate awareness and attitude of monitors in 206 small sized industries in Seoul and Kyeonggi area toward the occupational health programs using a specially prepared questionnaire. The industries had been running Government-Funded Subsidiary Program since 1998. The questionnaire was designed to be self-administered by respondents. Data were collected for two months from November to December, 1998. Major results are as follows: 1. Between two groups of industries, there were no significant differences in classification, scale, methods of production, existence of trade union, but in type of working pattern and facility ownership there were significant differences. 2. Between two groups of monitors, there were no significant differences in gender, age, marital status, rank, years of education, but in duration of working there was a significant difference. 3. In awareness and attitude of monitors toward their activities and occupational health programs, there was no significant difference by groups of industries, but a significant difference by type and scale of industry, gender, age, years of education 4. It was found that newly subsidized industries were more necessary to provide education programs on occupational health than old industries, and there was a significant difference between two groups of industries in the contents of education needed. In conclusion, it is desirable that the government should develop specific training programs for monitors on occupational health to provide them practical knowledge and specific skills which monitors can apply to their specific situation, and implement the training programs and continue Government-Funded Subsidiary Program for years until small scale industries would have been able to develop and keep their own health management system spontaneously.

• 대한공업교육학회지
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• v.32 no.2
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• pp.155-170
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• 2007

The purpose of this study was to estimate key competencies according to their levels in order to understand person's ability in automobile fields of school and workplace. For this study, key competencies consisting of mathematical skills, problem solving skills, communications skills, self management and development skills, resources recycling skills, interpersonal relationship skills, technological skills, information skills, organizational understanding skills were determined. We examined the related literatures to determine the level of key competencies and diverse opinions were accommodated with visits to workplace in order to enhance the validity of the results of this study. Based on the results of this study, it was concluded that the estimation of key competencies according to their levels was possible and valid.

• The Journal of Korean Medicine
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• v.24 no.1
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• pp.29-40
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• 2003

Object : This research was performed to investigate the protective effect of Aurantii Immaturus Fructus against ischemic damage using PC12 cells and global ischemia in gerbils. Methods : To observe the protective effect of Aurantii Immaturus Fructus on ischemia damage, viability and changes in activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and production of malondialdehyde (MDA) were observed after treating PC12 cells with Aurantii Immaturus Fructus during ischemic insult. Gerbils were divided into three groups : a normal group, a 5-min two-vessel occlusion (2VO) group, and an Aurantii Immaturus Fructus administered after 2VO group. The CCAs were occluded by microclip for 5 minutes. Aurantii Immaturus Fructus was administered orally for 7 days after 2VO. The histological analysis was performed at 7 days after the surgery. For histological analysis, the brain tissue was stained with 1% cresyl violet solution. Results : The results showed that 1. Aurantii Immaturus Fructus had a protective effect against ischemia in the CAI area of the gerbil hippocampus 7 days after 5-minute occlusion, 2. In the hypoxia/reperfusion model using PC12 cells, the Aurantii Immaturus Fructus had a protective effect against ischemia in the dose of $0.2{\;}\mu\textrm{g}/ml,{\;}2{\;}\mu\textrm{g}/ml{\;}and{\;}20{\;}\mu\textrm{g}/ml$ 3. Aurantii Immaturus Fructus increased the activities of glutathione peroxidase and catalase, 4. The activity of superoxide dismutase (SOD) was increased by ischemic damage, which might represent self protection. This study suggests that Aurantii Immaturus Fructus has some neuroprotective effect against neuronal damage following cerebral ischemia in vivo with a widely used experimental model of cerebral ischemia in Mongolian gerbils, and it also has protective effects on a hypoxia/reperfusion cell culture model using PCq2 cells. Conclusions : Aurantii Immaturus Fructus has protective effects against ischemic brain damage at the early stage of ischemia.

• The Journal of Korean Medicine
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• v.24 no.1
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• pp.110-121
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• 2003

Objective : This research was performed to investigate the protective effect of Angelicae Dahuri Radix against ischemic damage using PC12 cells and global ischemia in gerbils. Methods : To observe the protective effect of Angelicae Dahuri Radix on ischemia damage, viability and changes in activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and production of malondialdehyde (MDA) were observed after treating PC12 cells with Angelicae Dahuri Radix during ischemic insult. Gerbils were divided into three groups : a normal group, a 5-min two-vessel occlusion (2VO) group, and an Angelicae Dahuri Radix administered after 2VO group. The CCAs were occluded by microclip for 5 minutes. Angelicae Dahuri Radix was administered orally for 7 days after 2VO. The histological analysis was performed at 7 days after surgery. For histological analysis, the brain tissue was stained with 1% cresyl violet solution. Results : 1. Angelicae Dahuri Radix has a protective effect against ischemia in the CA1 area of the gerbil hippocampus 7 days after 5-minute occlusion, 2. In the hypoxia/reperfusion model using PC12 cells, Angelicae Dahuri Radix has a protective effect against ischemia in the dose of $0.2\mu\textrm{g}/ml$, $2\mu\textrm{g}/ml$ and $20\mu\textrm{g}/ml$, 3. Angelicae Dahuri Radix increased the activities of glutathione peroxidase and catalase. 4. The activity of superoxide dismutase (SOD) was increased by ischemic damage, which might represent self protection. This study suggests that Angelicae Dahuri Radix has some neuroprotective effect against neuronal damage following cerebral ischemia in vivo with a widely used experimental model of cerebral ischemia in Mongolian gerbils, and it also has protective effects on a hypoxia/reperfusion cell culture model using PC12 cells. Conclusions : Angelicae Dahuri Radix has protective effects against ischemic brain damage at the early stage of ischemia.

• Journal of Korean Society of Rural Planning
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• v.20 no.1
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• pp.91-104
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• 2014

Self-sufficiency rate of food in South Korea is almost at the lowest level among OECD countries, and the decrease tend of farmland is expected to be continued. In this situation, Korea experienced a rapid change in the international price of grain due to changes in amount of grain production and food weaponization. It also started to reinforce the appropriateness of maintenance of farmland above certain standard. As one of policy means for this situation, this study selected "the use of idle farmland" as a subject, and started to consider the way to suggest the policy projects using idle farmland. The result of this study is summarized as follows. Firstly, this study defined the meaning of idle farmland, and set up the scope of idle farmland needed to this study. Secondly, this study analyzed the domestic major policy projects with prevention function of idle farmland as well as the policy projects related to idle farmland. It also investigated "recycling measures of idle farmland" that is recently promoted in Japan, and deducted the direction for developing a new model. Lastly, It suggested 9 policy project models that are finally able to use idle farmland. Suggested models are designed to access from various directions. This study suggested contents, entity, target and characteristics of projects in order to set up proper directions in promoting policy projects using idle farmland.