• Asian-Australasian Journal of Animal Sciences
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• 제15권1호
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• pp.7-10
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• 2002

The breeding data relating to Hariana herd spread over 18 years (1979-96) were analysed to estimate genetic, phenotypic and environmental changes in characters of economic importance which might have taken place during the several years of selective breeding practiced in the herd. The average genetic changes in a given character were estimated by four methods. The phenotypic trends observed for different economic traits were not significant. On changing the method of estimation, magnitude and direction of genetic trends changed. Comparison of estimates of genetic trends by different methods showed that adjustments for biases due to non-random allotment of dams with respect to their age and merit suggested by Powell and Freeman (1974) were useful for increasing the precision of the estimates. Hence, this method was found to be the best method for estimation of genetic trends. The estimate of genetic trends by this method were 4.03${\pm}$6.21 days, 3.24${\pm}$5.33 kg, 0.15${\pm}$0.43 days, 0.09${\pm}$0.59 days, 0.01${\pm}$0.02 kg and 0.01${\pm}$0.01 kg for age at first calving, first lactation milk yield, first lactation length, first calving interval, first lactation milk yield per day lactation length and first lactation milk yield per day of calving interval, respectively.

• 한국패류학회지
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• 제28권4호
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• pp.343-347
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• 2012

속성장을 위한 선발 1세대 전복 (Selected abalone : SA)의 성장률을 조사하기 위하여 동일 사육 환경 조건에서 대조구 전복 (Control abalone : CA) 과 비교실험을 하였다. 그 결과 각 성장형질 (각장, 각폭 및 중량) 은 SA구가 CA구보다 유의하게 빠른 것으로 나타났다. 45일째 및 90일째의 평균 각장에 있어 SA구가 CA구 보다 각각 31.7% 및 17.8% 빠르게 나타났으며, 중량에 있어서도 각각 43.7% 및 25.6% 빠른 것으로 나타났다. 더욱이 90일째의 각장과 중량의 상대성장 비교에 있어서도 SA구가 CA구 보다 유의적으로 높은 상관관계를 나타내었다. 이러한 결과는 선발육종기술을 이용하여 양식 전복의 성장률을 증대할 수 있는 가능성을 시사하고 있다.

• 한국패류학회지
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• 제32권4호
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• pp.249-254
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• 2016

북방전복을 대상으로 성별에 따른 성장형질의 변이를 이용한 선발육종의 가능성 여부를 확인하고자, 실제로 암수 1:1 수정을 통하여 10가계를 생산 관리하여 성별에 따른 성장형질의 표현형 측정과 유전모수를 추정하였다. 그 결과 성비율은 1:1로 일반적 전복류의 성비와 같은 결과를 나타내었으며, 성별에 따른 성장은 모든 성장형질에 있어 암컷이 수컷보다 유의적으로 큰 것으로 나타났다 (p < 0.05). 이러한 결과는 선발을 할 때 성별에 따른 성장차이를 이용하여 양식전복의 성장률 향상을 할 수 있는 가능성을 시사하고 있다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1995년도 제9회 식물생명공학 심포지움 식물육종과 분자생물학의 만남 The 9th Plant Biotechnology Symposium -Breeding and Molecular Biology-
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• pp.133-170
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• 1995

The major objectives of fruit breeding lie in improvement of cultivar, easy to be cultivated and of high quality, in order to produce unexpensive, delicious fruit both for fresh fruit market and processing. Recently, fruit breeding in Korea has contributed to breeding of several superior cultivars in major fruit crops, resulting in appreciable improvement in qualities such as skin color, taste and fruit-bearing habit concerned with productivity. In spite of accomplishments mentioned above, the need for both highly disease-resistant cultivars and long-keeping, physiological disorder-resistant cultivars to meet long distance transsportation in the temperate fruit crops of apples, oriental pears, stone fruits such as peaches, and grapes grown in Korea is rapidly pressing more than ever, as cultivars of today susceptible to pests and diseases and vulnerable to physiological disorders are very expensive and time-consuming in post-harvest handling and management. Thus, imporvements made in the above problems through breeding level will lead to the really enhanced productivity in fruit industry. The major impediments of tree size, the long length of juvenile period and the highly heterogeneous genetic composition to the improvement of fruit crops are responsible for the lower amount and rate of improvements of fruit crops as compared to annuals. Considering the expected limitations of the above problems to be solved through conventional breeding methods and strategy, a turning point of breeding a near perfect cultivar would be laid down if innovative breakthroughs in biological technology will be realized in applying some of the techniques of genetic manipulation at the molecular level to the cultivar improvement of fruit crops, such as the selective insertion of DNA carrying genes that govern desirable characteristics. More than anything else, those traits such as fruiting habit deciding productivity, elements of fruit qualities conditioned by monogene, and disease-and pest-resistance of vital importance for successful fruit growing are urgently desired to be improved by advancement of biotechnology for they are more than difficult and need long period to be attained through conventional breeding method.

• Animal Bioscience
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• 제36권7호
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• pp.991-1002
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• 2023

Objective: This study aimed to elucidate the underlying gene regions responsible for productive, phenotypic or adaptive traits in different ecological types of Tibetan sheep and the discovery of important genes encoding valuable traits. Methods: We used whole-genome resequencing to explore the genetic relationships, phylogenetic tree, and population genetic structure analysis. In addition, we identified 28 representative Tibetan sheep single-nucleotide polymorphisms (SNPs) and genomic selective sweep regions with different traits in Tibetan sheep by fixation index (Fst) and the nucleotide diversity (θπ) ratio. Results: The genetic relationships analysis showed that each breed partitioned into its own clades and had close genetic relationships. We also identified many potential breed-specific selective sweep regions, including genes associated with hypoxic adaptability (MTOR, TRHDE, PDK1, PTPN9, TMTC2, SOX9, EPAS1, PDGFD, SOCS3, TGFBR3), coat color (MITF, MC1R, ERCC2, TCF25, ITCH, TYR, RALY, KIT), wool traits (COL4A2, ERC2, NOTCH2, ROCK1, FGF5, SOX9), and horn phenotypes (RXFP2). In particular, a horn-related gene, RXFP2, showed the four most significantly associated SNP loci (g. 29481646 A>G, g. 29469024 T>C, g. 29462010 C>T, g. 29461968 C>T) and haplotypes. Conclusion: This finding demonstrates the potential for genetic markers in future molecular breeding programs to improve selection for horn phenotypes. The results will facilitate the understanding of the genetic basis of production and adaptive unique traits in Chinese indigenous Tibetan sheep taxa and offer a reference for the molecular breeding of Tibetan sheep.

• 생명과학회지
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• 제28권1호
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• pp.130-139
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• 2018

말을 활용한 경주는 고대 유럽의 여러 국가들에서 마차 경주 혹은 산악 경주 등의 형태로 이루어졌으며, 고대 그리스 올림픽에서 마차 경주가 정식 종목으로 채택되었다. 서러브레드종은 17세기부터 속도, 체력, 그리고 경주 능력을 위해 선택적으로 교배되었다. 그 결과, 18세기부터 귀족들이 향유하는 스포츠로서 서러브레드종을 활용한 경주가 시행되었다. 이후 여러 국가에서 각기 다양한 형태로 발달하여 현재 크게 평지 경주, 장애물 경주, 마차 경주 등으로 발달하였다. 서러브레드 경주마는 300여 년 동안 강력한 선발 육종 전략에 의하여 선택되어 왔기에, 현재 우수한 경주 능력을 갖추고 있다. 말산업은 번식, 조련, 경마 등을 통하여 막대한 경제적 효과를 유발하기에, 말의 경주 능력을 유지하고 극대화하는 것이 필요하다. 최근에 많은 양의 게놈 데이터를 처리하기 위해 차세대 시퀀싱(Next Generation Sequencing; NGS)이 개발되었으며, 이 분석 기술의 현저한 발전을 토대로 우수한 형질을 가진 동물 육종 전략을 쉽게 수행 할 수 있게 되었다. 따라서 뛰어난 경주 능력을 가진 경주마를 선발 육종하기 위해서는 최신 유전체 분석 기술을 활용하는 전략이 필요하다. 본 논문에서는 경주마의 경주 능력을 향상시키기 의한 유전체 분석의 현재의 노력을 알아보고, 마지막으로 경주마와 제주마에서 유전체 분석을 활용하는 전략을 제안할 것이며, 대한민국의 생명자원인 제주마의 선발 육종 전략을 제안할 것이다. 말 산업은 기술, 사회 및 경제 분야에서 인간에게 강력한 파급 효과를 주는 동물 중 하나이다. 우리는 국내 고부가가치 말의 원천적인 유전 정보를 확보하고 선발 육종 할 수 있는 체계적인 기술을 확립하여 생산, 연구 업무 등에 대한 일자리 확보에 기여할 수 있기를 기대한다.

• 한국수산과학회지
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• 제42권6호
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• pp.600-603
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• 2009

Genetic and phenotypic parameter estimates for measurement traits were obtained from pacific oyster Crassostrea gigas at nine months old. For the growth-related traits among nine months old pacific oyster, heritabilities of shell length, shell height, shell width, total weight, body weight and shell weight were estimated as 0.4855, 0.5248, 0.0884, 0.7236, 0.7726 and 0.6957, respectively. Genetic correlations among the growth-related traits of pacific oyster at nines month old, shell length, shell height, shell width, total weight, body weight, shell weight were showing highly positive correlations. Breeding value on growth-related traits of pacific oyster at nine months old were estimated as shell length -7.044-11.870, shell height -11.380-18.370, shell width -1.234-2.831, total weight -8.339-17.140, body weight -1.813-3.507 and shell weight -4.422-8.837. The results show that there is quite substantial additive genetic variance for measurement traits in pacific oyster that can be exploited through selective breeding.

• 한국환경과학회지
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• 제30권3호
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• pp.289-293
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• 2021

This study was conducted to investigate the distribution of duck production in duck housing as a function of the breeding period. The items in duck production distribution included body weight, feed intake, feed conversion ratio, and average duck production. All data were based on eight cycles (June and December, 2017; February, June, August, September, and November, 2018; February, 2019) at Farm Site 1 through 7. The most optimal results in the duck production distribution were observed at Site 1 for weight gain, feed intake, and feed efficiency. This could be explained by the fact that Site 1 was well managed in environmental improvement and sustainability. Based on these data, selective alternatives to improve duck production in duck farms are recommended as follows: first, proper management and recording of litter used as a flooring material are required, and second, continuous management such as temperature, relative humidity and ventilation is needed. Lastly, it is necessary to change duck facilities by introducing environmental management techniques. Furthermore, efforts to improve the overall facilities and management of duck breeding farms through additional field studies are needed in the future.

• Asian-Australasian Journal of Animal Sciences
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• 제33권2호
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• pp.203-211
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• 2020

Objective: Staphylococcus aureus (S. aureus) is one of the major microorganisms responsible for subclinical mastitis in dairy cattle. The present study was designed with the aim to explore the DNA methylation patterns using the Fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) techniques in a S. aureus-infected mouse model. Methods: A total of 12 out-bred Institute of Cancer Research female mice ranging from 12 to 13 weeks-old were selected to construct a mastitis model. F-MSAP analysis was carried out to detect fluctuations of DNA methylation between control group and S. aureus mastitis group. Results: Visible changes were observed in white cell counts in milk, percentage of granulocytes, percentage of lymphocytes, CD⁴⁺/CD⁸⁺ ratio (CD⁴⁺/CD⁸⁺), and histopathology of mice pre- and post-challenge with S. aureus. These findings showed the suitability of the S. aureus-infected mouse model. A total of 369 fragments was amplified from udder tissue samples from the two groups (S. aureus-infected mastitis group and control group) using eight pairs of selective primers. Results indicated that the methylation level of mastitis mouse group was higher than that in the control group. In addition, NCK-associated protein 5 (Nckap5) and transposon MTD were identified to be differentially methylated through secondary polymerase chain reaction and sequencing in the mastitis group. These observations might play an important role in the development of S. aureus mastitis. Conclusion: Collectively, our study suggests that the methylation modification in Nckap5 and transposon MTD might be considered as epigenetic markers in resistance to S. aureus-infected mastitis and provided a new insight into S. aureus mastitis research in dairy industry and public health.

• 한국산림과학회지
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• 제85권2호
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• pp.309-315
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• 1996

원목재배용(原木裁培用) 표고우량균주(優良菌株)를 육성(育成)하기 위해 균주수집(菌株蒐集)에 의한 선발육종(選拔育種)과 교배계(交配系)를 이용한 교잡육종(交雜育種)을 실시한 결과는 다음과 같다. 1. 국내외(國內外)에서 수집(蒐集)한 35종의 균주에 대한 원목재배를 실시하여 1세대(世代)(5년간(年間)) 버섯수확량(收穫量)이 신갈나무(Quercus mongolica) $1m^3$당(當) 139kg을 보이는 FRI 177 등 3개의 고온성균주(高溫性菌株)와 120kg을 나타낸 FRI 188 등 2개의 중온성균주(中溫性菌株) 및 96kg의 FRI 187 등 저온성(低溫性) 7개 균주를 우량품종(優良品種)으로 선발하였다. 2. Di-mon교배법(交配法)을 이용하여 제조한 교잡균주중(交雜菌株中) 실내검정(室內檢定)을 통하여 독립계통(獨立系統)으로 확인된 12균주(菌株)에 대하여 원목재배(原木栽培)를 실시하여 1세대(世代) 버섯수확량이 원목(原木) $1m^3$당(當) 108kg에 달하는 고온성균주(高溫性菌株) FRI 182와 103kg을 보이는 저온성균주(低溫性菌株) FRI 184 등 2개를 우량종균(優良種菌)으로 선발하였다. 3. 고온성, 중온성 및 저온성 등 온도형(溫度型)에 따른 생산성(生産性)의 유의차(有意差)는 없었으나, 계통간(系統間)의 생산성차이(生産性差異)는 인정(認定)되었다. 한편 원목재배(原木栽培)와 톱밥재배간(栽培間)의 생산성에는 어떤 상관관계(相關關係)도 보이지 않았다.