• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.278-285
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• 2008

An efficient transformation method for soybean [Glycine max (L.) Merr.] using meristematic tissues of germinating seeds has been established. The embryonic axes were excised from germinating seeds of Korean soybean cultivar, Iksannamulkong and 0.5-2 cm long segment containing meristematic tissues were prepared by cutting hypocotyl region. The explants were inoculated with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector with the bar gene as a selectable marker gene and a ${\beta}-glucuronidase$ (GUSINT) reporter gene, and then co-cultured for 7 days on co-cultivation medium (CCM). The meristematic tissues were cultured on shoot induction medium (SIMP6) supplemented with 0.4 mg/l $N_6-benzylaminopurine$ (BAP) and 0.1 mg/l indolebutyric acid (IBA) in the presence of 6 mg/l L-phosphinotricin (PPT) for 2 weeks and the surviving explants were transferred to shoot elongation medium (SEMP6). Transformation was confirmed by Southern blot analysis and the transformation efficiencies ranged from 1.48 to 2.07%. The new modified transformation method was successfully implemented for obtaining several transgenic lines with SMV-CP gene. It is expected that this method could efficiently be used for the transformation of recalcitrant soybean cultivars.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.252-260
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• 2009

Rice is the most important cereal crop not only in supplying the basic staple food for more than half of the world's population but also as a model plant for functional genomic studies of monocotyledons. Although rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5 mg/L L-cysteine, 1 mM sodium thiosulfate, 1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5 mg/L silver nitrate). Co-cultivation temperature ($23.5^{\circ}C$ for 1 day, $26.5^{\circ}C$ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.507-514
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• 2010

Peanut(Arachis hypogaea L.) is one of the major oilseed crops. The peanut oil consists of palmitic, oleic and linoleic acids, which are present at levels of 10%, 36-67% and 15-43%, respectively. High oleate mutant of peanut F435 contains 80% oleate and as little as 2% linoleate in seed oil. Previous study indicated that delta 12 fatty acid desaturase is a major enzyme controlling the oleate content in seeds of oilseed crops. F435 sequence alignment of their coding regions disclosed that an extra A(adenine) was inserted at the position +2,823 bp of delta 12 fatty acid desaturase gene. This study was to develop molecular marker (SNP marker) co-segregating with the high oleate trait. Chopyeong ${\times}$ F435 $F_2$ 41 population were investigated using molecular marker and fatty acid assay (NIR and gas chromatography). Finally, this marker segregates Chopyeong type 26 lines, heterotype 9 lines and F435 type 6 lines. These results in our study suggested that SNP marker conform fatty acid assay.

• Journal of The Korean Association of Information Education
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• v.26 no.3
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• pp.197-207
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• 2022

In this paper, we develop a machine learning based block code generation and recommendation model for the purpose of reducing cognitive load of learners during coding education that learns the learner's block that has been made in the block programming environment using natural processing model and fine-tuning and then generates and recommends the selectable blocks for the next step. To develop the model, the training dataset was produced by pre-processing 50 block codes that were on the popular block programming language web site 'Entry'. Also, after dividing the pre-processed blocks into training dataset, verification dataset and test dataset, we developed a model that generates block codes based on LSTM, Seq2Seq, and GPT-2 model. In the results of the performance evaluation of the developed model, GPT-2 showed a higher performance than the LSTM and Seq2Seq model in the BLEU and ROUGE scores which measure sentence similarity. The data results generated through the GPT-2 model, show that the performance was relatively similar in the BLEU and ROUGE scores except for the case where the number of blocks was 1 or 17.

• Journal of Plant Biotechnology
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• v.50
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• pp.56-62
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• 2023

Transgenic lilies have been obtained using Agrobacterium tumefaciens (AGL1) with the plant scale explants, followed by DL-phosphinothricin (PPT) selection. In this study, scales of lily plants cv. "red flame" were transformed with the pCAMBIA3301 vector containing the gus gene as a reporter and the blpR gene as a selectable marker, as well as a gene of interest showing herbicide tolerance, both driven by the CaMV 35S promoter. Using a 20-minute infection time and a 5-day cultivation period, factors that optimized and demonstrated a high transformation efficiency were achieved. With these conditions, approximately 22-27% efficiency was observed for Agrobacterium-mediated transformation in lilies. After transformation with Agrobacterium, scales of lilies were transferred to MS medium without selective agents for 2 weeks. They were then placed on selection MS medium containing 5 mg/L PPT for a month of further selection and then cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets after transferring into hormone-free MS medium. Also, most survived putatively transformed plantlets indicated the presence of the blpR gene by PCR analysis and showed a blue color indicating expression of the gus gene. In conclusion, when 100 scales of lily cv. "red flame" are transformed with Agrobacterium, approximately 22-27 transgenic plantlets can be produced following an optimized protocol. Therefore, this protocol can contribute to the lily breeding program in the future.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.4
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• pp.81-91
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• 2017

The purpose of this study is to establish a system of planning elements necessary for effective healing garden. We suggested new planning parts and elements for healing garden through analyzing prior literature and evaluated the importance of planning elements through expert surveys. The results of the study are stated as follows. First, we suggested five planning parts and 19 planning elements. $\LARGE{\cdot}$ Planning parts: Physical environment, social environment, cognitive environment, functional environment, psychological environment. $\LARGE{\cdot}$ Planning elements: Plant planting, garden view, hydroponic space, shade space, work space, recreation space, community space, exercise space, landmark element, sign system, safe facilities and materials, disaster safety facility, universal design, accessible path, economical maintenance, sight openness, privacy guaranteed, variety of resting space, artistic element. Second, as a result of analyzing the importance of planning elements, the first place was the planting, the second place was the safety facilities for the disaster, and the third was diversity of rest space. In addition, the artistic elements and the landmark elements were ranked 18th and 19th, respectively. Third, we evaluated the level of importance of planning elements and classified it into three groups. The most important A group included two elements(plant planting, disaster safety facility), the next important B group included eleven elements(variety of resting space, community space, hydroponic space, universal design, safe facilities and materials, economical maintenance, accessible path, privacy guaranteed, garden view, work space, sight openness) and the less important C group included six elements(shade space, exercise space, recreation space, sign system, artistic element, landmark element). We suggest that, in creating a healing garden, the two elements in A group are essential elements and the eleven elements in B group should be importantly applied. In addition, the six elements in C group are recognized as arbitrarily selectable elements due to the relatively low importance levels.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.56-61
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• 1997

This research was conducted to investigate morpological and growth characteristics of 358 Coix lacryma-jopbi mayuen STAF collected in Korea. The test collections contained 76% medium wide-type leaf, 59% medium size-type seed, 34% large size-type seed, 70% elliptical-type shell, 50% brown shell color, 92% low stem color and hardness of seed coat averaged $3.4kg/cm^2$ with the range of $1.1{\sim}18.7kg/cm^2$. 24% adaptable plant height ranged from 156cm to 170cm, days to heading after seeding averaged 83.2% with the range of $74{\sim}94$ days, early maturating varietes was 24.9% below 80 days. Rate to leaf blight 48.5% with the range of $9{\sim}92%$ and rate to stem borer averaged 8% with the range of $0{\sim}17%$. The weight of 1000 seeds showed positive correlation with days to flowering and plant height and number of seeds per plant showed positive corrleation with percentage of ripness, but weight of 1000 seeds showed negative correlation with occurrence of leaf blight and stem borer plant. Therefore we are expecting useful germplasm and selectable index for effective breeding.

• Journal of The Korean Association For Science Education
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• v.42 no.1
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• pp.19-32
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• 2022

The need for polar education was further emphasized, depending on the importance of the pole, which is the best place for climate change detection and prediction, and treasure trove of future technology and resources. Therefore, this study analyzed the general cognitive and affective characteristics of elementary school students' polar literacy, and in addition, analyzed the cognitive and affective characteristics according to the level of diversity about polar experience. The items developed for the study were revised through a pilot survey of 43 fifth graders. They consisted of questions about gender, polar experience, scientific literacy, polar knowledge, polar literacy skills, polar literacy beliefs, and polar literacy attitudes. The types of questions used are selectable, reliable, and Likert (4 points), for a total of 66 questions. The students who participated in the study were 323 fifth grade elementary students. The study found that students were more interested in the dramatic consequences of polar changes than the scientific causes and processes associated with it. This is confirmed through the fact that they are more interested in and familiar with polar creatures suffering from polar changes than understanding ice, which is the main feature of and the central mechanism of polar changes. Students also recognized the issue of polar climate change as a global issue other than their own. They believe that what happens in the Arctic and Antarctica will affect the whole world, but not significantly to himself and his community. The level of knowledge about polar region and the ability to analyze and infer were not significantly related to each other, and students with a higher level of diversity of experience about polar region had a better understanding of polar science and technology. In this research, it is meaningful to check the characteristics related to the students' polar region and to use it as a basic data to show the direction in which polar literacy education should proceed in the future.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.152-160
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• 2005

This study was carried out to investigate selectable criteria in evaluating waxy corn $F_1$ hybrids for developing good eating quality waxy corn variety. The physicochemical property analysis of 6 waxy corn $F_1$ hybrids - Chalok1, Chalok2, Heugjeomchal, Yeonnongl, Chalok4, and Suwon45- showed a range of $11.2\~13.1\%$ for crude protein, $5.1\~6.0\%$ for crude fat, $91.8\~92.6\%$ for amylopectin, and $4.5\~6.6\%$ for free sugar content. The pericarp thickness which is one of the most important characteristics related to tenderness in waxy corn was ranged $34\~47{\mu}m$ in 4 waxy corn hybrids - Yeonnongl, Chalok4, Suwon45, and Heugjeomchal. On the other hand, it was ranged $64\~81{\mu}m$ in Chalok1 and Chalok2. The amylogram analysis by rapid visco analyzer showed that in fresh waxy corn hybrid (DAP25), all amylogram properties except setback were higher in Yeonnongl, Chalok4, and Suwon45 compared to those of Chalokl, Chnlok2, and Heugjeomchal. However, in matured waxy corn hybyids (DAP45), the result was the opposite - the amylogram properties were higher in Chalokl, Chalok2, and Heugjeomchal than those of Yeonnongl, Chalok4, and Suwon45. The texture analysis showed that gumminess, chewiness, and hardness increased dramatically with the time after the cooking in Chalokl and Beugjeomchal. On the other hand, these above pyoperties did not change as rapidly with the time in Yeonnongl, Chalok4, and Suwon45. Gumminess, chewiness, and hardness did not increase much within 6 hours after steamingr but increased significantly 32 hours after steaming. Therefore, we have reached a conclusion that texture analysis of cooked waxy corn should be carried out 6 hours after steaming. In the sensory evaluation, Yeonnongl, Chalok4, and Suwon45 revealed higher palatability -6.8, 7.1, and 6.9 respectively - than. that of Chnlokl, Chalok2, and Heugjeomchal. The palatability analysis of 6 waxy corn hybrids showed palatability positively correlating with free sugar content,100-kernel weight, kernel length, kernet width, and consistency, but negatively correlating with pericarp thickness, hardness, gumminess, and chewiness.