• Journal of Embryo Transfer
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• v.10 no.1
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• pp.73-82
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• 1995

large scale production of cloned embryos requires the technology of multiple generation nuclear transplantation(NT) using NT embryos as the subsequent donor nuclei. The purposes of this study were producing the second generation cloned rabbit embryos, and also to determine the electrofusion rate and in vitro developmental potential comparatively in the cloned embryos of the first and second NT generation. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulbecco's phosphate buffered saline(D-PBS) containing 10% fetal calf serum(FCS) at 47 hours after hCG injection In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gi /S transition of 32-cell stage. The first generation NT embryos which were developed to 8-cell were synchronized in Gi /S transition phase of the following 16-cell stage and used as donor nuclei for second generation Synchronization of the cell cycle of blastomeres was induced, first, using an inhibitor of microtuble polymerization, colcemid for 10 hours to arrest blastomeres in M phase, and secondly, using a DNA synthesis inhibitor, aphidicolin for 1.5 to 2 hours to arrest them in Gi /S transition boundary. The recipient cytoplasms were obtained by removing the nucleus and the first polar body from the oocytes collected at 14 hours after hCG injection. The separated donor blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were electrofused by electrical stimulation of three pulses for 60 $\mu$sec at 1.25 kV /cm in 0.28 M rnannitol solution The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10% FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. Following in vitro culture of the first and second generation cloned embryos to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The results obtained were summarized as follows: 1. The electrofusion rate was found to be similar as 79.4 and 91.5% in the first and second generation NT rabbit embryos, respectively. 2. The in vitro developmental potential to blastocyst stage of the second generation NT embryos (23.3%) was found significantly(p<0.05) lower, compared with that of the first generation NT embryos (56.8%). 3. The mean blastomeres counts of embryos developed to blastosyst stage following in vitro culture for 120 hours and also their daily cell cycles during the culture period were decreased significantly (p<0.05) to 104.3 cells and 1.33 cylces in the second NT generation, compoared with 210.4 cells and 1.54 cycles in the first NT generation, respectively.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.5 no.4
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• pp.653-660
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• 2001

In this paper, we designed 2303.15MHz frequency synthesizer for the purpose of the phase noise prediction. For the modeling of phase noise generated in the designed system through introducing the noise-modeling method suggested by Lascari we analyzed a variation of phase noise as according as that of offset frequency. Especially, for the third-order system of the PLL among some kinds of phase noise generated from VCO we analyzed the aspect of 1/f-noise appearing troubles in the low frequency band. Since it is difficult to analyze mathematically 1/f-noise in the third-order system of the PLL, introducing the concept of pseudo-damping factor has made an ease of the access of the 1/f-noise variance. we showed a numerical formula of 1/f-noise variance in the third-order system of the PLL which is compared with that of 1/f-noise variance in the second-order system of the PLL. As a result, In case of txco we found the reduce rapidly along the offset frequency after passed through that phase-noise was -160dBc/Hz before passed through a loop at 10kHz offset frequency and -162.6705dBc/kHz after passed through the loop, -180dBc/Hz at 100kHz offset frequency and -560dBc/kHz after passed through the loop. We can notice that the variance of third-order system more occurs (or the variance of second-order system in connection with noise bandwidth and variance factor of second-order and third-order system.

• Macromolecular Research
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• v.9 no.4
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• pp.238-246
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• 2001

Properties of ternary blends of nylon 6 (Ny6), a thermotropic liquid crystalline polymer (TLCP, poly(ester amide), 20 wt%) and a maleic anhydride grafted polypropylene (2 wt%) (MAPP) were studied under various processing conditions. TLCP was pre-blended with MAPP first and then the binary one blended again with Ny6. The processing temperature of the second mixing was varied. Thermal properties show the partial miscibility of the ternary blend. The morphology of the TLCP phase in the first blending shows mostly in the fibril bundle shape, but varies between droplets and oriented fibrils after the second processing. Some of TLCP phase lost the fibril morphology during the second processing stage. The morphology variation invokes the change in tensile properties. Low extrusion temperature (270$\^{C}$) provides more fibril shapes, which are associated with less deformation in the second stage. The processing temperature effect was more evident when the draw ratio was high. High drawing was applicable due to the stabilizing action of tile compatibilizer.

• International Journal of Ocean System Engineering
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• v.1 no.4
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• pp.205-210
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• 2011

High data-rate underwater communications is demanded. This demand imposes stringent requirements on underwater communication equipment of phase-locked-loop (PLL). Phase noise in PLL is unwanted and unavoidable. In this paper, we investigate the PLL phase noise effect on high order QAM for underwater communication systems. The phase noise model using power spectral density is adopted for performance evaluation. The phase noise components considered in PLL are reference oscillator, voltage controlled oscillator (VCO), filter and divider. The filters in PLL noise are assumed to be second order active and passive low pass filters. Through simulation, we analyze the phase noise characteristics of the four components and then investigate the performance improvement factor of each component. Consequently, we derive specifications of VCO, phase detector, divider to meet performance requirement of high data-rate communication using QAM under phase noise influence.

• Tribology and Lubricants
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• v.16 no.1
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• pp.33-38
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• 2000

Abstract- In this paper tribological characteristics of solid and liquid phase sintered W/C-35%Ni tappets were investigated. Three test methods were performed to investigate the wear and surface damage mechanism of sintered tappets. First, block-on-ring wear test was performed to investigate the wear characteristics under pure sliding condition. Second, simplified cam and tappet tests (called component wear test hereafter) were carried out to simulate the real contact history of cam and tappet. Also, after the test, contact surfaces were analyzed with scanning electron microscope to study the wear mechanism. As a final screening, engine dynamo tests were performed. Results showed that in the block on ring sliding wear test, solid phase sintered specimens showed superior wear resistance to liquid phase sintered specimens. The component wear tests and engine dynamo tests also showed the same results. Therefore, in these tests, solid phase sintered tappet material revealed superior wear resistant properties to liquid phase sintered one.

• Journal of Magnetics
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• v.1 no.2
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• pp.82-85
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• 1996

Magnetic properties and microstructures of melt spun misch metal-ferroboron alloys were investigated. The major phase is the tetragonal (rare earth)$_2Fe_{14}B$ phase. Magnetic properties showed coercivity of 5.6 kOe, remanence of 7.85 kG, and so energy product 8.9 MGOe. Microsturctures in optimum properties showed that matrix was composed of Ce-rich phase while second phase La-rich-oxygen phase with less amount of Fe element than matrix, and triple junction with La-rich phase contrary to matrix.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.48 no.10
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• pp.1349-1355
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• 1999

A power distribution facility map is drawn on cadastral map. Besides, grid lines are added on the map for sectionalization. For automatic recognition of the map, we first extract recognizing regions. In this paper, we propose an extraction method of recognizing regions by partially extending thinned image. The proposed method is consist of three phases, binarization phase, thinning phase and partial extending phase. The first phase generate a binary image using threshold value which is obtained by histogram analysis. The binary image contains many part of recognizing regions, but not all. The second phase generate thinned image which is generated by appling thinning operator to the binary image. And the third phase extends thinned image from terminal point until satisfying termination condition. The proposed method is tested on several power distribution facility maps, and the results are presented.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1997.04a
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• pp.41-45
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• 1997

To design a control system, it is a elementary point that the stability of the system should be guaranteed. Also, the phase of the system plays an important role for its frequence performance. In this paper, we present two stability criterion of repetitive control system with phase-lead and lag compensator. First, the stability criterion for the servo control system with phase-lead and lag compensator is shown by using small-gain theorem. Second, for the repetitive control system with the compensator, the stability criterion, also, is determined by using small-gain theorem. Two stability criterions show the same results that the stability depends on a coefficient of the phase-lead and lag compensator under some condition in servo control system and repetitive control system.

• Journal of Power Electronics
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• v.16 no.2
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• pp.498-511
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• 2016

This paper presents a single-phase five-level inverter controlled by two novel pulse width modulation (PWM) switching techniques. The proposed PWM techniques are designed based on minimum switching power loss and minimum total harmonic distortion (THD). In a single-phase five-level inverter employing six switches, the first proposed PWM technique requires four switches to operate at switching frequency and two other switches to operate at line frequency. The second proposed PWM technique requires only two switches to operate at switching frequency and the rest of the switches to operate at line frequency. Compared with conventional PWM techniques for single-phase five-level inverters, the proposed PWM techniques offer high efficiency and low harmonic components in the output voltage. The validity of the proposed PWM switching techniques in controlling single-phase five-level inverters to regulate load voltage is verified experimentally using a 100 V, 500 W laboratory prototype controlled by dspace 1103.

• Journal of the Korean Institute of Telematics and Electronics
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• v.15 no.5
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• pp.13-18
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• 1978

A delay switching PLL (DSPLL) is proposed for improvement of the frequency acquisition Performance (pull-in range) while keeping a narrow bandwidth LPF. It has, between the phase detector and the LPF, just a simple RC delay circuit, a switch and another phase detector controlling the switching time. For the common second order PLL, the pull-in capability of the DSPLL is analyzed approximately, without considering additive white noise effect, and verified experimentally. It is shown that the delay switching extends the pull-in range significantly, as much as a half of lock-range. At the phase tracking mode, the delay switching does not function, to make the DSPLL be a normal PLL.