• The korean journal of orthodontics
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• v.33 no.2 s.97
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• pp.113-120
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• 2003

The aim of this study was to evaluate the effect a chewable tablet containing sodium fluoride and xylitol (DenPo, Hamizzle, Bucheon, Kyungki) on the oral hygiene state in the orthodontic patients.'rho subjects consisted of 30 adult orthodontic patients, who have worn the fixed orthodontic appliance at least 6 months. They were instructed to use the tablet forth a day, Gingival index (GI), plaque index (PI), and sulcus bleeding index (SBI) of upper and lower anterior and posterior regions were measured before using the tablet (T0), 2 weeks after using the tablet (T1), and 4 weeks after using the tablet (T2). The data were analyzed with Wilcoxon signed ranks tests. The results were as follows ; 1 PI was decreased significantly in the T1 and T2 stage compared with T0 stage in the both anterior and posterior regions (p<0.05). Although PI was decreased in the T2 stare compared with T1, there was not statistical significance (p>0.05). 2. GI and SBI showed similar results compared with PI. In the T1 and T2 stage, there was statistically significant decrease in the GI and SBI (p<0.05), This study showed that the DenPo tablet could be used as an adjunct to improve oral hygiene in the orthodontic patients. The decrease of the indices nay suggest that DenPo tablet could be effective in the plaque removal and prevention of gngival inflammation in the orthodontic patients.

• Restorative Dentistry and Endodontics
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• v.29 no.5
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• pp.479-484
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• 2004

Immunoinhibitory protein extracted from sonicated Treponema denticola have been shown to suppress cell cycle progression of human lymphocytes. To study in detail about the effect of this microorganism on the function of lymphocytes. we investigated the levels of Interleukin-2 (IL-2) and Interleukin-4 (IL-4) production by T lymphocytes before and after the addition of $12.5{\;}\mu\textrm{g}/ml$ T. denticola sonicated extracts. In this study. levels of IL-2 and IL-4 produced from T cells pretreated with sonicated extracts were evaluated by using the quantitative sandwich enzyme immunoassay technique. In response to phytohemagglutinin (PHA) stimulation. T cell produced increased levels of IL-2 and IL-4. However. the expressions of both cytokines were significantly inhibited when PHA activated-T cells were pre-exposed to sonicated T. denticola extracts (p < 0.05). These findings suggest that the T. denticola sonicated extracts induced-immunosuppression in Th1 and Th2 cell functions could be a part of the pathogenic mechanism of the endodontic failure associated with this microorganism.

• Journal of Preventive Medicine and Public Health
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• v.32 no.1
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• pp.88-94
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• 1999

Objectives. In order to gain a better understanding of the mechanism of DMF toxicity, recent studies have focused on hepatic drug metabolizing enzymes. In this study, we investigated the effects of DMF on the induction of P450 and the activities of other related enzymes in rat liver microsomes. Methods. DMF was administered to male Sprague Daweley rats by intraperitoneal injection at 0(control), 450(D1), 900(D2), 1,800(D3) mg DMF/kg body weight in olive oil once a day for three days. Hepatic P450 was measured by method of Omura and Sato. We evaluated selective assays for the three drug metabolizing cytochrome P450 isoenzymes 1A1, 2B1 and 2E1. Results. The content of microsomal protein, P450 and b5 were tended to be decreased in DMF treated group, but they were not statistically significant. The activity of NADPH-cytochrome P450 reductase was significantly increased dose dependently(p<0.01), but the activity of NADH-b5 reductase was decreased in the treated group(p<0.01). The activities of PROD and EROD were not significant between control and treated group. The activities of pNPH in the DMF treated groups were higher than that of the control group(p<0.01). When Western immunoblottings were carried out utilizing three monoclonal antibodies which were specific against P4501A1/1, P4502B1/2 and P4502E1, the strong density band corresponding to P4502E1 was observed with the microsomes obtained from the rats treated with DMF. But there were no significant increased in the P4501A1/2 and P4502B1/2 band densities in immunoblotting. Conclusions. These result suggested that P4502E1 was inducible by DMF and P4502E1 isozyme might be responsible for the hydroxylation of DMF to HMMF.

• Journal of Preventive Medicine and Public Health
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• v.32 no.3
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• pp.383-394
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• 1999

Objectives: This study was conducted to identify the factors influencing the mortality of Koreans over the age of 40 by a nested case-control study. Methods: The cohort consisted of the beneficiaries of Korea Medical Insurance Corporation for Government Employees & Private School Teachers and Staff(KMIC) who received health examinations of KMIC in 1992 and 1993 retrospectively. At that time, they were more than 40 years old. The cases were 19,258 cohort members who had died until December 31, 1997. The controls were 19,258 cohort members who were alive until December 31, 1997. Controls were matched with age and sex distribution of the cases. The data used in this study were the funeral expenses requesting files, and the files of health examinations and health questionnaires gathered in 1992 and 1993. To assess the putative risk factors of death, student t-test, chi-square test, multiple logistic regression analysis were used. Results : In multiple logistic regression analysis, independent risk factors of death were as follows; systolic blood pressure, diastolic blood pressure, blood glucose, AST, urine glucose, urine protein, alcohol drinking(frequency), cigarette smoking and perceived health status, intake of restoratives and blood transfusion showed positive associations with death; coffee consumption showed negative associations with death; and body mass index and serum total cholesterol showed J-shaped association with death. Conclusions: Regarding the direction of association, the result of analysis on the data restricted to '96-'97 was same as that of '93-'97. But in some variables such as obesity, serum cholesterol, the odds ratios of death in the data of '96-'97 were higer than those of '93-'94, which suggested that the data of '93-'94 was bearing effect-cause relationship. We concluded that it suggested further researches using long-term follow-up data to be needed in this area.

• Journal of Life Science
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• v.16 no.2 s.75
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• pp.289-296
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• 2006

In order to establish highly efficient gene transfer condition at early stage of soybean transformation, various experiments were performed and compared their efficiencies by transient GUS analysis; those conditions are genotype determination of Korean soybean cultivars for amenability to Agro-infection, appropriate agar and selective agent concentration, orientation of explant placement, hormone pre-culture, and liquid selection condition. In the genotype screen of Korean soybean varieties, 14 amenable genotypes were selected. For efficient Agrobacterium washing, cefotaxime was chosen and hygromycin at the concentration of 10 and 15 ppm was used as selection agent in the media. Agar concentration was slightly better in 0.6% and 0.8% for both shoot and callus formation, and explant placement with adaxial side down showed high frequency of GUS expression. For wounding treatment, oriental needle was efficient than scalpel for shoot formation and gene transfer. To increase the frequency of gene transfer, hormone pre-treatment was applied. BA at the concentration of 5 and 10 ppm resulted in better survival at the late stage of selection in shoot elongation media. Selection in liquid media after hormone pre-treatment seemed to be effective to remove the escaped non-transformants at early stage of procedure. Considering the results obtained, Eunhakong could be the first choice as a material for soybean transformation among Korean soybean genotypes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.3
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• pp.197-208
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• 2007

This study was carried out to investigate effects of increasing moisture content with or without supplementing inoculant (Lactobacillus plantarum) in TMR (total mixed ration) on its feed value. In case of exposing TMR to air, the lower the moisture level of TMR was, the less its apparent condition was changed. The time of spreading of molds tended to be faster in TMR with the higher moisture level. And also the odor was influenced by moisture content and inoculant supplement that is, sour odor was smelled from 24 hour after exposing TMR containing 35% and 50% moisture to air, but TMR supplemented with inoculant had sweet odor. The inner temperature of TMR containing 35% and 50% moisture without inoculant tended to increase continually after the lapse of 6 hours when the TMR was exposed to air. The inner temperature of TMR containing 35% moisture with inoculant tended to increase dramatically after the lapse of 48 hours when exposed to air, but that of TMR containing 50% moisture with inoculant tended to increase after 6 hours. The pH of TMR containing 15% was consistent regardless of exposing time to air, but that of TMR containing 35% and 50% moisture considerably increased after 12 and 24 hours, respectively. The concentration of $NH_3-N$ of TMR supplemented with inoculant was increased from 6 hours after exposure to air, while that or TMR without inoculant increased from 12 hours. Nutrient content or TMR tended to be increased with the increase of exposing time to air and storage time under sealed condition.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.4
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• pp.624-629
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• 2014

Obesity is the leading metabolic disease in industrialized countries and is closely associated with coronary heart disease, hypertension, diabetes, and cancer. The objective of this study was to evaluate the anti-adipogenic effects of two roasted coffee beans, Vietnam robusta (VR) and Ethiopia Mocha Sidamo G2 (ES), as well as fermented coffee beans with Monascus ruber (MR) mycelium on differentiation of 3T3-L1 preadipocytes. Treatments with 1,000 ${\mu}g/mL$ of hot water extract from coffee beans significantly reduced intracellular lipid accumulation. In addition, VR more effectively inhibited transcription factors such as $PPAR{\gamma}$, $C/EBP{\alpha}$, FAS, and aP2 compared to ES. Further, ES fermented with MR showed more effective anti-adipogenic activity than non-fermented ES. These results suggest that VR and ES inhibit adipocyte differentiation which may contribute to their anti-adipogenic properties.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.3
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• pp.487-496
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• 2013

Green coffee beans (CB, Indonesian Mandheling) were fermented with three kinds of mushrooms (Phellinus linteus, PL; Hericium erinaceum, HE; Ganoderma lucidum, GL) or two kinds of mycelia from molds (Monascus purpureus, MP; Monascus ruber, MR) using solid-state culture to enhance physiological activity. After the roasting of fermented green coffee beans, roasted coffees were extracted with a hot-water decoction or 95% ethanol reflux. Yields from hot water extracts (HW, 17.7~25.3%) were higher than those from ethanolic extracts (EE, 9.5~12.2%). Hot-water extracts of roasted coffees from green coffee beans fermented with two molds (MP-CB-HW and MR-CB-HW) showed higher total polyphenols, flavonoids, and DPPH free radical scavenging activity than roasted coffees from non-fermented (CB-HW) or fermented green coffee beans with the three mycelia from mushrooms. MR-CB-HW also had the most potent macrophage stimulating and mitogenic activity (1.32 and 1.40-fold of CB-HW, respectively). In addition, MP-CB-EE and MR-CB-EE did not show any cytotoxicity to the RAW 264.7 cell at a concentration of $100{\mu}g/mL$, and these extracts significantly inhibited nitric oxide (NO) production from the LPS-stimulated RAW 264.7 cell line (38.6 and 37.0% of the LPS-treated group). Meanwhile, the chlorogenic acid concentrations of MP-CB-HW or MR-CB-HW highly increased (to 76.21 or $76.73{\mu}g/mL$, respectively), but caffeine concentrations were not affected by solid-state fermentation. In conclusion, the physiological activities of roasted coffees were enhanced by the solid-state culture of green coffee beans with M. purpureus or M. ruber, suggesting that these roasted coffees could possibly serve industrial applications as functional coffee beverages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.2
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• pp.201-207
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• 2014

The changes of growth characteristics and antioxidant activity for selection of optimum germinated temperature on common bean (Phaseolus vulgaris L.). Common beans (IT100888, IT102849, and IT231267) were cultivated at $20^{\circ}C$, $23^{\circ}C$ and $25^{\circ}C$ during 5 days of germination. The range of whole length, hypocotyls length, thickness, abnormal germination and yield rate of sprouts was 7.27~27.62 cm, 3.10~18.86 cm, 1.80~2.27 mm, 5.54~18.34% and 205.95~ 618.71%, respectively. Antioxidant activities of common beans with germination temperature investigated. Common beans (IT100888, IT102849, and IT231267) germinated at $20^{\circ}C$, $23^{\circ}C$ and $25^{\circ}C$ during 5 days, and then extracted with 80% ethanol, and analyzed for total polyphenol content, DPPH and ABTS radical scavenging activity. Total polyphenol content increased from 474 mg GA eq/100 g sample for IT231267 to 1364 mg GA eq/g sample for $23^{\circ}C$ of germination. DPPH radical scavenging activity of IT102849 increased from 189mg Trolox eq./100 g sample ($20^{\circ}C$) to 1073mg Trolox eq./100 g sample ($23^{\circ}C$) also ABTS radical scavenging activity of IT234267 increased from 479 mg Trolox eq./100 g sample ($20^{\circ}C$) to 1134 mg AA eq/100 g ($23^{\circ}C$). These results suggest that germination temperature for increasing antioxidant activities may be $23^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.327-338
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• 2020

In recent years, the temperature of Korea has been rapidly increasing due to global warming. Over the past 40 years, the temperature of Korea has risen by about 1.26℃ compared to that in the early 1980s. By region, the west region of the Gangwon Province was the highest at 1.76℃ and the Jeonnam Province was the lowest at 0.96℃. As the temperature continues to rise, it is expected that the rice yield will decrease in the future using the current standard cultivation method. As a result of global warming, the periods in which rice cultivation could be possible in regions each year has increased compared those to the past, showing a wide variety from 110 days in Taebaek to 180 days in Busan and Gwangyang. In addition, the transplanting time was delayed by 3-5 days in all regions. The average annual yield of rice showed an increasing trend when we analyzed the average productivities of developed varieties for cooked rice since the 1980s, especially in the early 1990s, which showed a rapid increase in productivity. The relationship between the average temperature at the time of development and the rice yield was divided into the periods before and after 1996. The higher the average temperature, the lower the yield of the developed varieties until 1996. However, since 1996, the increase in the average temperature did not show a trend in the productivity of the developed varieties. The climate change adaptability of developed rice varieties was investigated by analyzing the results of growing crops nationwide from 1999 to 2016 and the change in the annual yields of developed varieties and recently developed varieties as basic data to investigate the growth status of the crops in the country. As a result of annual comparisons of the yields of Taebongbyeo (2000) and Ungwangbyeo (2004) developed in the early 2000s for Odaebyeo, which was developed in the 1980s, the annual yields were relatively higher in varieties in the 2000s despite the increase in temperature. The annual yields of Samgwangbyeo (2003) and Saenuribyeo (2007), which were recently developed as mid-late-type varieties, were higher than those of an earlier developed variety called Chucheongbyeo, which was developed in the 1970s. Despite the rapid increase in temperature, rice cultivation technology and variety development are well adapted to climate change. However, since the biological potential of rice could reach its limit, it is necessary to develop continuous response technology.