KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Growth Characteristics of Sprouts and Changes of Antioxidant Activities in Common Bean (Phaseolus vulgaris L.) with Cultivated Temperature

재배온도에 따른 강낭콩 싹나물의 재배특성 및 항산화 활성

  • 김현영 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 구성철 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 강범규 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 이영훈 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 김현태 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 윤홍태 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 백인열 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 정헌상 (충북대학교 식품공학과) ;
  • 최만수 (농촌진흥청 국립식량과학원 기능성작물부)
  • Received : 2014.04.01
  • Accepted : 2014.05.22
  • Published : 2014.06.30
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Abstract

The changes of growth characteristics and antioxidant activity for selection of optimum germinated temperature on common bean (Phaseolus vulgaris L.). Common beans (IT100888, IT102849, and IT231267) were cultivated at $20^{\circ}C$, $23^{\circ}C$ and $25^{\circ}C$ during 5 days of germination. The range of whole length, hypocotyls length, thickness, abnormal germination and yield rate of sprouts was 7.27~27.62 cm, 3.10~18.86 cm, 1.80~2.27 mm, 5.54~18.34% and 205.95~ 618.71%, respectively. Antioxidant activities of common beans with germination temperature investigated. Common beans (IT100888, IT102849, and IT231267) germinated at $20^{\circ}C$, $23^{\circ}C$ and $25^{\circ}C$ during 5 days, and then extracted with 80% ethanol, and analyzed for total polyphenol content, DPPH and ABTS radical scavenging activity. Total polyphenol content increased from 474 mg GA eq/100 g sample for IT231267 to 1364 mg GA eq/g sample for $23^{\circ}C$ of germination. DPPH radical scavenging activity of IT102849 increased from 189mg Trolox eq./100 g sample ($20^{\circ}C$) to 1073mg Trolox eq./100 g sample ($23^{\circ}C$) also ABTS radical scavenging activity of IT234267 increased from 479 mg Trolox eq./100 g sample ($20^{\circ}C$) to 1134 mg AA eq/100 g ($23^{\circ}C$). These results suggest that germination temperature for increasing antioxidant activities may be $23^{\circ}C$.

본 연구는 강낭콩 싹나물의 재배시 재배온도에 따른 싹나물의 재배특성 및 항산화 활성을 분석하여 강낭콩을 이용한 채소용 싹나물 재배방법 구명과, 강낭콩 싹나물이 가진 성분 및 활성 등을 분석하여 최적 재배 조건을 구명하기 위하여 수행하였다. 1. 강낭콩 싹나물 재배를 위하여 $20^{\circ}C$, $23^{\circ}C$ 및 $25^{\circ}C$의 온도에서 5일간 재배한 결과 전장 및 배축장은 $23^{\circ}C$에서 재배하였을 경우 가장 우수하게 재배되었으며, 배축직경의 경우는 온도와 상관없이 모두 유사하게 재배되었다. 2. 재배온도별 강낭콩 싹나물의 불완전발아율 및 수율을 측정한 결과 재배온도별로 큰 차이를 보였으며, $20^{\circ}C$ 및 $25^{\circ}C$에서 재배 시 50%가 넘는 불완전발아율을 보였으나, $23^{\circ}C$에서는 10% 내외로 나타났다. 3. 강낭콩 싹나물의 재배온도별 조지방 및 조단백질 함량을 분석한 결과 재배온도 및 강낭콩 품종에 따라 큰 차이를 나타내지 않았다. 4. 재배온도($20^{\circ}C$, $23^{\circ}C$ 및 $25^{\circ}C$) 및 일수(0일, 3일, 5일 및 7일)별 강낭콩 싹나물의 폴리페놀 함량 및 라디칼 소거능을 측정한 결과 재배온도 및 일수가 증가함에 따라 폴리페놀 함량 및 라디칼 소거능이 증가하였으며, 특히 $23^{\circ}C$에서 재배 시 가장 높은 폴리페놀 함량 및 라디칼 소거능을 나타냈다.

Keywords

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