• Biomedical Science Letters
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• v.6 no.1
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• pp.73-82
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• 2000

Blunt-end DNA fragments can be inserted in two different orientations. Conventionally, their directions are determined by restriction enzyme digestion or by DNA sequencing, however, these methods are often limited in their use due to the lack of appropriate enzyme sites or large sample numbers, respectively. In the present study, a novel strategy and the corresponding protocol for the simple determination of insert orientation is introduced. Using conventional sequencing primers and PCR primers that have been used for amplification of the insert, single clones, which have inserted the fragment in the desired orientation, were easily identified by this PCR-based method. The fidelity of this system was confirmed by cloning of a tar urocortin cDNA, which is a recently discovered neuropeptide. Recombinant clones identified by this method were further shown to be fully functional, and using these, for the first time, urocortin was recombinantly expressed in eukaryotic cells.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.15 no.3
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• pp.133-144
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• 2020

This study examines the indirect effects of new product development(NPD) on the relationship between entrepreneurial orientation(EO) and business performance is contingent on organization size. Although previous studies have revealed that EO has a positive effect on business performance and NPD, there have been no research attempts to comprehensively grasp the causal path of EO, NPD, and business performance. Therefore, this study analyzed the causal path focusing not only on final financial business performance affected by EO but also on the mediating role of new product development, which is an intermediate performance. Based on the sample of 3,299 firms from the 2018 Entrepreneurship Survey(Corporate), we analyzed the EO-NPD-business performance relationship and explored whether such an indirect effect of EO on business performance differs depending on the size of the firm. The results showed that EO has a positive impact on business performance and NPD. In addition, NPD positively mediates the relationship between EO and business performance under the condition of large size firm, although NPD does not have a direct effect on business performance. With the findings, the current study has principal implications for practicing managers. The result of this study is not to suggest that NPD triggered by EO is a panacea for improving final business performance. The study suggests a need to equip organizational capabilities to successfully commercialize new products and appeal to the market and customers, beyond simply developing new products seeking newness based on EO.

• Journal of Technology Innovation
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• v.21 no.2
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• pp.57-84
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• 2013

New product development(NPD) is imperative management activity for growth and survival of the firm. In the context of the small and medium-sized enterprises(SMEs), the firm became further dependent upon NPD with the increasing turbulence in environments. This research suggests the role of entrepreneurship orientation(EO) and absorptive capacity(ACAP) that affect the success rate of NPD in SMEs. Although each of them has been highlighted as critical resources in the aspect of the sustainable competitive advantage, the relationship and the effect between EO and ACAP have received scant attention. Based on the sample of 261 Korean SMEs, this study examines the direct/indirect effect of EO and ACAP on NPD performance. While innovativeness of EO has the direct effect on NPD performance, both risk-taking and proactiveness of EO show no significance. While realized ACAP has the positive influence on NPD performance, potential ACAP shows no significant influence. In addition, we found that the impact of innovativeness on NPD performance was being partially mediated by ACAP whereas the relationship between proactiveness and NPD performance being fully mediated by ACAP. These findings would provide generic insights that both exploration and exploitation regarding knowledge and resources in SMEs are necessary in order to increase NPD performance and two activities have to align with EO.

• Journal of Korean Society for Geospatial Information Science
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• v.17 no.1
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• pp.21-35
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• 2009

KOMPSAT-2 is the seventh high-resolution image satellite in the world that provides both 1m-grade panchromatic images of the GSD and 4m-grade multispectral images of the GSD. It's anticipated to be used across many different areas including mapping, territory monitoring and environmental watch. However, due to the complexity and security concern involved with the use of the MSC, the use of KOMPSAT-2 images are limited in terms of geometric images, such as satellite orbits and detailed mapping information. Therefore, this study aims to produce DEM and orthoimage by using the stereo images of KOMPSAT-2, and to explore the applicability of geo-spatial information with KOMPSAT -2. Orientation interpretations were essential for the production of DEM and orthoimage using KOMPSAT-2 images. In the study, they are performed by utilizing both RPC and GCP. In this study, the orientation interpretations are followed by the generation of DEM and orthoimage, and the analysis of their accuracy based on a 1:5,000 digital map. The accuracy analysis of DEM is performed and the results indicate that their altitudes are, in general, higher than those obtained from the digital map. The altitude discrepancies on plains, hills and mountains are calculated as 1.8m, 7.2m, and 11.9m, respectively. In this study, the mean differences between horizontal position between the orthoimage data and the digital map data are found to be ${\pm}3.081m$, which is in the range of ${\pm}3.5m$, within the permitted limit of a 1:5,000 digital map. KOMPSAT-2 images are used to produce DEM and orthoimage in this research. The results suggest that DEM can be adequately used to produce digital maps under 1:5,000 scale.

• Journal of Korea Entertainment Industry Association
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• v.15 no.6
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• pp.153-163
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• 2021

This study has a main purpose that closely examines the structural relationship among A Study on the Structural Relationship of Achievement Goal Propensity, Sports Confidence, and Perceived Performance Using the Mentoring System of High School Leaders. In order to achieve it, the sample from high school student participants were accumulated by using convenience sampling that chose taekwondo championship in national. The samples were 500 people. and we excluded unsuitable sheet like that were decided as untruthful one and an omission and unclear. So the final samples were 465 people. On the basis of that, The statistical techniques, spss 24.0 and amos 24.0 program have been used for the analysis as per research purpose. The statistical analysis used in the research for a data analysis includes frequency analysis, confirmatory factor analysis, reliability analysis, correlation analysis, and structure equation model analysis. The research has drawn the following conclusions, based on the above mentioned research method and its procedures: First, The mentoring influences the achievement goal orientation. Second, The mentoring influences the sport confidence. Third, The achievement goal orientation influences the perceived performance. Fourth, The sport confidence influences the perceived performance.

• Journal of Korean Neurosurgical Society
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• v.65 no.2
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• pp.315-324
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• 2022

Objective : The aim of this study was to verify the equivalence and effectiveness of the tablet-administered Korean Repeatable Battery for the Assessment of Neuropsychological Status (K-RBANS) for the prevention and early detection of dementia. Methods : Data from 88 psychiatry and neurology patient samples were examined to evaluate the equivalence between tablet and paper administrations of the K-RBANS using a non-randomly equivalent group design. We calculated the prediction scores of the tablet-administered K-RBANS based on demographics and covariate-test scores for focal tests using norm samples and tested format effects. In addition, we compared the receiver operating characteristic curves to confirm the effectiveness of the K-RBANS for preventing and detecting dementia. Results : In the analysis of raw scores, line orientation showed a significant difference (t=-2.94, p<0.001), and subtests showed small to large effect sizes (0.04-0.86) between paper- and tablet-administered K-RBANS. To investigate the format effect, we compared the predicted scaled scores of the tablet sample to the scaled scores of the norm sample. Consequently, a small effect size (d≤0.20) was observed in most of the subtests, except word list and story recall, which showed a medium effect size (d=0.21), while picture naming and subtests of delayed memory showed significant differences in the one-sample t-test. In addition, the area under the curve of the total scale index (TSI) (0.827; 95% confidence interval, 0.738-0.916) was higher than that of the five indices, ranging from 0.688 to 0.820. The sensitivity and specificity of TSI were 80% and 76%, respectively. Conclusion : The overall results of this study suggest that the tablet-administered K-RBANS showed significant equivalence to the norm sample, although some subtests showed format effects, and it may be used as a valid tool for the brief screening of patients with neuropsychological disorders in Korea.

• Journal of the Semiconductor & Display Technology
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• v.4 no.1 s.10
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• pp.29-34
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• 2005

Tungsten oxide($WO_{3}$) is suitable to materials for photochromic window in the visible region. The resistivities of CdS, $WO_{3}$, and $WO_{3}$/CdS films prepared by thermal evaporation method were $4.61\times 10\^{3}$, $7.59\times10^{3}$, and $6.29\times10^{3}$ $\omega$ cm. And x-ray diffraction patterns of CdS, $WO_{3}$/CdS films showed a preferred orientation of hexagonal(002), and the monoclinic(020) structure, respectively. The optical transmission were measured that the cut-on wavelength were 510nm, 380nm for CdS and $WO_{3}$ films respectively, and the transmission spectrum of $WO_{3}$/CdS was shifted into the visible region. Photoluminescence(PL) spectra showed the two peaks at 2.8 eV and 3.2 eV for the as-grown sample($WO_{3}$/CdS ($500{\AA}$), but the other sample($WO_{3}$/CdS ($1000{\AA}$)) had a peak energy value of 2.8 eV. The photochromism of $WO_{3}$/CdS films showed that the excitation of electron-hole pairs and subsequent coloration is shifted into visible-light range. And the spectral behavior of coloration turned out to be proportional to the excited electron-hole pairs creation rate of CdS film. This result is interpreted in terms of charge carrier injection from the CdS-layer into the $WO_{3}$ films. We found a value of about 2.8 eV of $WO_{3}$/CdS film which is somewhat higher than peak energy of 2.54 eV using CBD prepared by Bechinger et. al.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.39 no.1
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• pp.49-60
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• 2002

We propose an algorithm for extracting the boundary of a desired object with shape information obtained from sample images. Considering global shape obtained from sample images and edge orientation as well as edge magnitude, the Proposed method composed of two steps finds the boundary of an object. The first step is the approximate segmentation that extracts a rough boundary with a probability map and an edge map. And the second step is the detailed segmentation for finding more accurate boundary based on the SEEL (seed-point extraction and edge linking) algorithm. The experiment results using IR images show robustness to low-quality image and better performance than conventional segmentation methods.

• Korean Journal of Materials Research
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• v.21 no.8
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• pp.439-443
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• 2011

The crystal structure and magnetic properties of a new solid solution type ferrite $(Fe_2O_3)_5-(Al_2O_3)_{3.4}-(Ga_2O_3)_{0.6}-SiO$ were investigated using X-ray diffraction and M$\"{o}$ssbauer spectroscopy. The results of the X-ray diffraction pattern indicated that the crystal structure of the sample appears to be a cubic spinel type structure. The lattice constant (a = 8.317 ${\AA}$) decreases slightly with the substitution of $Ga_2O_3$ even though the ionic radii of the Ga ions are larger than that of the Al ions. The results can be attributed to a higher degree of covalency in the Ga-O bonds than in the Al-O and Fe-O bonds, which can also be explained using the observed M$\"{o}$ssbauer parameters, which are the magnetic hyperfine field, isomer shift, and quadrupole splitting. The drastic change in the magnetic structure according to the Ga ion substitution in the $ (Fe_2O_3)_5(Al_2O_3)_{4-x}(Ga_2O_3)_xSiO$ system and the low temperature variation have been studied through a M$\"{o}$ssbauer spectroscopy. The M$\"{o}$ssbauer spectrum at room temperature shows the superpositions of two Zeeman patterns and a strong doublet. It shows significant departures from the prototypical ferrite and is comparable with the diluted ferrite. The doublet of spectrum at room temperature appears to originate from superparamagnetic clusters and also the asymmetry of the doublet appears to be caused by the preferred orientation of the crystallites. The M$\"{o}$ssbauer spectra below room temperature show various complicated patterns, which can be explained by the freezing of the superparamagnetic clusters. On cooling, the magnetic states of the sample were various and multi critical.

• Journal of the Korean Magnetics Society
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• v.19 no.4
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• pp.126-132
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• 2009

We have studied physical properties of MnAs thin films grown by Molecular-Beam Epitaxy as well as their post-growth annealing effects. The samples grown at $600^{\circ}C$ show the preferred crystal orientation of type-B independent of substrate whereas type-A is observed for the samples grown at below $200^{\circ}C$. The sample grown at $600^{\circ}C$ on GaAs(001) substrate is magnetized to only one direction even on the easy axis of magnetization. The magnetic properties are vastly enhanced after post-growth annealing for both MnAs/Si(001) sample with no ferromagnetism and ferromagnetic MnAs/GaAs(001) grown at $200^{\circ}C$.