• BMB Reports
• /
• 제28권2호
• /
• pp.100-106
• /
• 1995

S-adenosylmethionine (SAM) synthetase was purified to homogeneity from soybean (Glycine max) axes. The enzyme was purified 216-fold with a 1.5% yield by ammonium sulfate fractionation, acetone fractionation, ion exchange chromatography with DEAE-sephacel, gel filtration with Sephacryl S-300, and afffinity chromatography with ATP-agarose. The enzyme activity reached a maximum 3 days after germination. SAM synthetase had a subunit molecular weight of 57,000 daltons from a silver stained single band on SDS-PAGE. The molecular weight of the enzyme was 110,000 daltons from Sephacryl S-300 gel filtration. The enzyme was composed of two identical subunits. The $K_m$ values of the enzyme for L-methionine and ATP were 1.81 and 1.53 mM, respectively. The enzymatic activity was not affected by polyamines, agmatine, or SAM analogues, but was inhibited by SAM. The inhibition pattern was showed non-competitive for L-methionine and uncompetitive for ATP. The activity of SAM synthetase was inhibited by thiol-blocking reagents. The enzyme was induced by treatment with $10^{-3}$ M putrescine at germination. Experimental data revealed a possible novel regulation mechanism of polyamine biosynthesis through several endogenous intermediates.

• Journal of Microbiology and Biotechnology
• /
• 제16권7호
• /
• pp.1154-1157
• /
• 2006

It is known that overexpression of S-adenosyl-L-methionine (SAM) synthetase or exogenous addition of SAM enhances the production of actinorhodin, one of pigmented antibiotics found from Streptomyces coelicolor. In order to discover a novel compound as a signal molecule to produce actinorhodin instead of SAM, several compounds were synthesized based on the relationships between structures of the SAM analogues and their actinorhodin productivities. Of these, a few compounds showed better productivities of actinorhodin than SAM.

• 전기화학회지
• /
• 제16권4호
• /
• pp.217-224
• /
• 2013

본 연구에서는 금 나노입자로 전착된 전극위에 3-MPA로 개질된 자가조립 단층 전극의 순환전위법의 특성을 조사하였다. 또한 전극 주변에서 전자와 이온의 이동현상을 해석하였다. 반무한 확산모델을 채택하여, 물질전달 지배계에 대하여 지배방정식과 경계조건을 유도하였다. 자가조립단층 전극에 대한 순환전위법의 수치해를 구하기 위하여, 양함수 유한차분법을 적용하여 MATLAB 프로그램을 작성하여 해를 구현하였다. 자가조립으로 개질된 전극에 대한 본 CV 용 프로그램은 3-MPA으로 개질된 금 나노입자 SAM 전극의 순환전위법 실험결과를 잘 설명하고 있음을 확인할 수 있었다.

• 동국한의학연구소논문집
• /
• 제1권
• /
• pp.55-80
• /
• 1992

In order to examine that the effect of Sam Hwa San, circulating the vital energy of Sam Cho and controlling body fluid metabolism, gives any influence on renal function, changes in the urine flow, eletrolytes excretion, plasma aldosterone concentration and renin activity were observed after intravenous infusion of the Sam Hwa San extract in rabbit. Also in vitro effect of the herb extract on oxygen consumption in renal cortical slices and ATPase activity in kidney microsomes was measured. The following results were obtained : 1. The urine flow was markedly increased at 10 min after intravenous infusion of the Sam Hwa San extract($0.134{\pm}0.015$ vs. $0.433{\pm}0.046ml/min.kg$), but return ed to normal value after 40 min of infusion. 2. The glomerular filtration rate was significantly increased at 10 min after in travenous infusion of the Sam Hwa San extract, and the renal plasma flow at 10 and 20 min after infusion of the Sam Hwa San extract, following return to normal value. 3. $Na^+$ excretion was significantly increased during 10-40 min after intravenous infusion of the Sam Hwa San extract, although showed the maximal rate at 10-20 min. The fractional $Na^+$ excretion was also increased during 10-40 min. $K^+$ excretion was rapidly increased at 10 min after the intravenous Infusion of the Sam Hwa San extract and then gradually decreased to normal level at 40 min. The fractional $K^+$ excretion was significantly increased during 10-40 min after the intravenous infusion of the Sam Hwa San extract. 4. The plasma aldosterone concentration and renin activity were not altered by the infusion of the Sam Hwa San extract. 5. The ouabain-sensitive oxygen consumption of renal cortical slices was significantly reduced by the Sam Hwa San extract(0.5 and 1.0 vol.%). 6. The Na-K-ATPase activity of renal microsomes was strongly inhibited by the Sam Hwa San extract(0.5 and 1.0 vol.%). These results suggest that the Sam Hwa San causes a strong diuretic effect which results from reduction of Na reabsorption in renal tubule by a direct inhibition of Na-pump and, in part, from all increase in renal blood flow. In clinic, it is considered to obtain the therapeutic effect in body fluid metabolism disharmony to cause the circular disorder of vital energy.

• 한국도로학회:학술대회논문집
• /
• 한국도로학회 2005년도 춘계학술대회 논문집
• /
• pp.77-82
• /
• 2005

• 한국도로학회:학술대회논문집
• /
• 한국도로학회 2004년도 학술발표회 논문집
• /
• pp.347-352
• /
• 2004

• 한국도로학회:학술대회논문집
• /
• 한국도로학회 2004년도 춘계학술대회 논문집
• /
• pp.107-112
• /
• 2004

• Journal of Welding and Joining
• /
• 제29권1호
• /
• pp.14-19
• /
• 2011

• 한국의학물리학회:학술대회논문집
• /
• 한국의학물리학회 1999년도 Japanese Journal of Medical Physics
• /
• pp.399-400
• /
• 1999

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1998년도 추계학술발표회요약집
• /
• pp.49-49
• /
• 1998