• Journal of the Institute of Electronics Engineers of Korea SP
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• v.42 no.6
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• pp.19-26
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• 2005

Image interpolation in the wavelet domain usually takes advantage of the probabilistic models for the intrascale statistics and the interscale dependency. In this paper, we adopt the linear model for the absolute values of wavelet coefficients of interpolated image across scale to estimate the variances of extrapolated bands. The proposed algorithm uses randomly generated wavelet coefficients based on the estimated parameters for probabilistic model. Random number generation according to the estimated probabilistic model may induce the 'salt and pepper' noise in subbands. We reduce the noise power by Wiener filtering. We observe that the proposed method generates the histogram of the subband coefficients similar to the that of original image. Experimental results show that our method outperforms the previous wavelet-domain interpolation method as well as the conventional bicubic method.

• Journal of Microbiology and Biotechnology
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• v.21 no.7
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• pp.667-674
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• 2011

The gene for esterase (rEst1) was isolated from a new species of genus Rheinheimera by functional screening of E. coli cells transformed with the pSMART/HaeIII genomic library. E. coli cells harboring the esterase gene insert could grow and produce clear halo zones on tributyrin agar. The rEst1 ORF consisted of 1,029 bp, corresponding to 342 amino acid residues with a molecular mass of 37 kDa. The signal P program 3.0 revealed the presence of a signal peptide of 25 amino acids. Esterase activity, however, was associated with a homotrimeric form of molecular mass 95 kDa and not with the monomeric form. The deduced amino acid sequence showed only 54% sequence identity with the closest lipase from Cellvibrio japonicus strain Ueda 107. Conserved domain search and multiple sequence alignment revealed the presence of an esterase/ lipase conserved domain consisting of a GXSXG motif, HGGG motif (oxyanion hole) and HGF motif, typical of the class IV hormone sensitive lipase family. On the basis of the sequence comparison with known esterases/ lipases, REst1 represents a new esterase belonging to the class IV family. The purified enzyme worked optimally at $50^{\circ}C$ and pH 8, utilized pNP esters of short chain lengths, and showed best catalytic activity with p-nitrophenyl butyrate ($C_4$), indicating that it was an esterase. The enzyme was completely inhibited by PMSF and DEPC and showed moderate organotolerance.

• Journal of Life Science
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• v.23 no.12
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• pp.1486-1494
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• 2013

A high protease-producing strain was isolated and identified from the digestive tract of octopus vulgaris by detecting a hydrolysis circle of protease and its activity. The strain was identified by morphology observation, biochemical experiments, and 16S rRNA sequence analysis. The protease obtained from the strain was purified by a three-step process involving ammonium sulfate precipitation, carboxy methyl-cellulose (CM-52) cation-exchange chromatography, and DEAE-Sephadex A50 anion-exchange chromatography. The properties of protease were characterized as well. The strain Bacillus sp. QDV-3, which produced the highest activity of protease, was isolated. On the basis of the phenotypic and biochemical characterization and 16S rRNA gene-sequencing studies, the isolate was identified as follows: domain: Bacteria; phylum: Firmicutes; class: Bacilli; order: Bacillales; family: Bacillaceae; and genus: Bacillus. The isolate was shown to have a 99.2% similarity with Bacillus flexus. A high active protease designated as QDV-E, with a molecular weight of 61.6 kDa, was obtained. The enzyme was found to be active in the pH range of 9.0-9.5 and its optimum temperature was $40^{\circ}C$. The protease activity retained more than 96% at the temperature of $50^{\circ}C$ for 60 min. Phenylmethylsulfonyl fluoride (PMSF) inhibited the enzyme activity, thus confirming that this protease isolated from Bacillus sp. QDV-3 is an alkaline serine protease. Metal ions, $Mn^{2+}$ and $Mg^{2+}$, were determined to enhance the protease activity, whereas $Ba^{2+}$, $Zn^{2+}$, and $Cu^{2+}$ were found to inactivate the enzyme.

• Microbiology and Biotechnology Letters
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• v.49 no.3
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• pp.458-465
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• 2021

Tubulysins are a group of bioactive secondary metabolites from myxobacteria exhibiting strong anticancer activity against various cancer cell lines. In this study, we describe the identification of putative tubulysin biosynthetic gene clusters (tubA~tubF) in the genome sequences of two tubulysin-producing myxobacterial strains, Archangium gephyra MEHO_002 and MEHO_004. The inactivation of the putative tubulysin biosynthetic genes resulted in a tubulysin-production defect. The DNA sequences of the A. gephyra MEHO_002 and MEHO_004 tubulysin biosynthetic genes were 97% identical, and the amino acid sequences of the encoded proteins shared a similarity of 97-100%. The nucleotide sequences of the tubulysin biosynthetic gene clusters in MEHO_002 and MEHO_004 were 86% identical to that in Cystobacter sp. SBCb004 known as a tubulysin-producing myxobacterium, and the organization of the clusters was identical except for the lack of a tubZ gene in the clusters in MEHO_002 and MEHO_004. The amino acid sequences of the proteins encoded by each gene were 88-97% similar to those encoded by SBCb004, and the domain compositions of the proteins were also identical.

• Journal of the korean society of oceanography
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• v.39 no.3
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• pp.163-171
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• 2004

Sequences of the large subunit ribosomal (LSD) rDNA D1-D2 region of Alexandrium catenella(=A. sp. cf. catenella) and A. tamarense isolates, which were collected along the Korea coasts, were analyzed to understand their phylogenetic relationships and geographical distributions. All A. catenella and A. tamarense isolates belonged to the A. tamarense/catenella/fundyense complex and were grouped with the North American and temperate Asian ribotypes, respectively, regardless of the presence or absence of a ventral pore in the first apical plate. A consistent and peculiar characteristic that differentiated the Alexandrium isolates was amplification of a second PCR product with a lower molecular weight in addition to the predicted one; ten A. catenella isolates belonging to the temperate Asian ribotype yielded this additional PCR product. Sequence alignment revealed that the shorter PCR product resulted from an unusual large deletion of 87 bp in the LSD rDNA D1 domain. The North American and temperate Asian ribotypes were prevalent along the Korean coasts without geographical separation. Given the high genetic homogeneity among widely distributed Alexandrium populations, each ribotype appeared to be pandemic rather than to constitute a distinct regional population.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.4
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• pp.112-118
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• 2010

This paper describes a noise suppression system based on a normalization method using a time-delay neural network and line spectrum pair having a parameter of frequency domain. First, a time-delay neural network is trained using line spectrum pair values of noisy speech signals obtained by linear prediction analysis. After trained the time-delay neural network, the proposed system enhances speech signals that are degraded by a background noise. Accordingly, the proposed time-delay neural network restores from the line spectrum pair values of noisy speech signals to the line spectrum pair values of clean speech signals. It is confirmed that this system is effective for speech signals degraded by a background noise, judging from spectral distortion measurement.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.39 no.3
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• pp.11-22
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• 2002

The design of optimal filter yielding optimal texture feature separation is a most effective technique in many torture analyzing areas, such as perception of surface, object, shape and depth. But, most optimal filter design approaches are restricted to the issue of computational complexity and supervised problems. In this paper, Our proposed method yields new insight into the design of optimal Gabor filters for segmenting multiple texture images. The optimal frequency of Gator filter is turned to the optimal frequency of the distinct texture in frequency domain. In order to show the performance of the designed filters, we have attempted to build a various texture images. Our experimental results show that the performance of the system is very successful.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.3
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• pp.70-80
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• 2010

This study proposes the algorithm to automate image alignment and detect marginal bone destructions, based on subtraction radiography for dental radiographic images necessary for dental PACS, which hasn't been covered by related literatures. The proposed algorithm enables a quick and precise detection of marginal bone destructions around teeth including implant through the knowlege-based multi-template matching in reference to ROI(Region Of Interest) obtained from applicable teeth using information about their geometric forms to solve problems single-template matching is exposed to. Actually, the test showed that it was possible not only to quickly and precisely detect marginal bone destructions around teeth, but also to get more objective and quantitative results through the algorithm.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.44 no.3
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• pp.105-112
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• 2007

In general, the earlier methods for the design of MAXFLAT FIR filters have existent problems due to the approximation algorithms used to approach MAXFLAT(maximally flat) response in the passband and the stopband.. The proposed approach advanced by using of MAXSHCUT(maximally sharp cutoff) condition in this paper clearly overcomes these problems. In this approach, we use a key parameter represented with filter-order and cutoff-frequency parameters for obtaining the lowpass filters with the MAXFLAT and MAXSHCUT characteristics in the frequency domain. Consequently, this design technique leads to new MAXFLAT and MAXSHCUT FIR digital filter, which can achieve sharp-cutoff responses with the stopband attenuation exceeding 100 dB almost everywhere.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.46 no.1
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• pp.87-94
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• 2009

Noise estimation and suppression are a crucial factor of many speech communication and recognition systems. In this paper, proposed algorithm is based on the ratio of variance normalized of noisy power spectrum in time-frequency domain. Our proposed algorithm tracks the threshold and controls the trade-off between residual noise and distortion. This algorithm is evaluated by the ITU-T P.835 signal distortion (SIG) and segment signal to noise ratio (SNR), and is superior to the conventional methods.