• Mycobiology
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• v.41 no.2
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• pp.86-93
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• 2013

Sequence characterized amplified region (SCAR) markers are one of the most effective and accurate tools for microbial identification. In this study, we applied SCAR markers for the rapid and accurate detection of Phytophthora katsurae, the casual agent of chestnut ink disease in Korea. In this study, we developed seven SCAR markers specific to P. katsurae using random amplified polymorphic DNA (RAPD), and assessed the potential of the SCAR markers to serve as tools for identifying P. katsurae. Seven primer pairs (SOPC 1F/SOPC 1R, SOPC 1-1F/SOPC 1-1R, SOPC 3F/SOPC 3R, SOPC 4F/SOPC 4R, SOPC 4F/SOPC 4-1R, SOPD 9F/SOPD 9R, and SOPD 10F/SOPD 10R) from a sequence derived from RAPD fragments were designed for the analysis of the SCAR markers. To evaluate the specificity and sensitivity of the SCAR markers, the genomic DNA of P. katsurae was serially diluted 10-fold to final concentrations from 1 mg/mL to 1 pg/mL. The limit of detection using the SCAR markers ranged from $100{\mu}g/mL$ to 100 ng/mL. To identify the limit for detecting P. katsurae zoospores, each suspension of zoospores was serially diluted 10-fold to final concentrations from $10{\times}10^5$ to $10{\times}10^1$ zoospores/mL, and then extracted. The limit of detection by SCAR markers was approximately $10{\times}10^1$ zoospores/mL. PCR detection with SCAR markers was specific for P. katsurae, and did not produce any P. katsurae-specific PCR amplicons from 16 other Phytophthora species used as controls. This study shows that SCAR markers are a useful tool for the rapid and effective detection of P. katsurae.

• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.971-974
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• 2005

In this paper, we describe photonic sensor interface in SOPC(System on a programmable chip) platform. This platform uses device that has ARM922T processor and APEX FPGA area on a chip. We use two development kits. The one is embedded kit that using Intel's Xscale device, the another is SOPC kit that using Altera's Excalibur device. We implement some device logic that DMAC, ADCC, etc. and application.

• Journal of the Institute of Convergence Signal Processing
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• v.15 no.4
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• pp.169-175
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• 2014

NG-SDH system is connected with networks throughout optical fibers. Network synchronization controller is a necessary for the data synchronization in each optical transmission system. In this paper, we have design and implementation the network synchronization controller using SOPC(system on a programmable chip) design technic. For this network synchronization controller we use FPGA in Altera. FPGA includes 32bit CPU, DPRAM(dual port ram), digital input/output port, transmitter and receiver framer, phase difference detector. We also confirm that designed network synchronization controller satisfies the ITU-T G.813 timing requirements.

• Proceedings of the IEEK Conference
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• 2006.06a
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• pp.747-748
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• 2006

In this paper, we describe photonic sensor interface and driver program based on SOPC(System on a programmable chip) platform. This platform uses device that has ARM922T processor and APEX FPGA area on a chip. As for driver program development, three different methods are tried such as simple firmware, real-time OS based program and embedded Linux based program, and results are compared for SoC implementation.

• Journal of Institute of Control, Robotics and Systems
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• v.10 no.3
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• pp.261-266
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• 2004

Recently, a variety of intelligent robots are developed for the personal purpose beyond the industrial application. These intelligent robots have ranges of sensors, actuators, and control algorithms to their application. In this paper we propose a reconfigurable robot controller, $SR^2$c (The SOPC-based Reconfigurable Robot Controller), based on SOPC (System on a Programmable Chip), that can be reconfigurable easily by software. The proposed robot controller contains not only a processing module but also robot-specific IP's. To show a feasibility of the proposed robot controller, a small entertainment robot, Wizard-4 is implemented with a single chip controller as proposed in this paper.

• Proceedings of the KIEE Conference
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• 2005.07d
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• pp.2843-2845
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• 2005

This paper deals with the design of real-time embedded system using RTOS (real-time operating system) and SOPC (system on a programmable chip). It is, in general, known that RTOS has the problem of time delay caused by the multiple tasks and task management approach. Since the increase in time delay in real-time embedded system makes the overall system have the poor performance or the critical behavior of instability and unreliability, the method employed RTOS and SOPC is proposed to attack the above problems. The proposed system is implemented on the RC-motor controller and is verified by real experiment.

• Journal of the Korean Institute of Intelligent Systems
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• v.20 no.4
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• pp.580-585
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• 2010

In this paper, The object Tracking System is designed by SOPC based Nios II embedded processor and C2H compiler. And this system using single PTZ camera can effectively control IPs in the platform of SOPC based Nios II Embedded Processor and creating IP by C2H(C-To-Hardware) compiler for image-in/output, image-processing and devices of communication that can supply various monitoring information to network or serial. Accordingly, Special quality and processing speed of object tracking using high-quality algorism in the system is improved by hardware/software programming methods.

• Research in Plant Disease
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• v.18 no.2
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• pp.73-79
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• 2012

Phytophthora katsurae is a fungal pathogen responsible for chestnut ink disease. We designed two duplex primer sets (SOPC 1F/1R+KatI 3F/5R, SOPC 1-1F/1-1R+KatI 3F/5R) to detect P. katsurae. SOPC 1F/1R and SOPC 1-1F/1-1R primer pairs were designed for sequence characteristic amplification regions (SCAR) marker, and KatI 3F/5R primer pair was used for P. katsurae-specific primer designed from internal transcribed spacer (ITS) region. To assess the sensitivity of duplex PCR, genomic DNA was serially diluted 10-fold to make the final concentrations from 1 mg/ml to 1 ng/ml. The sensitivity for two primer sets were 1 ${\mu}g/ml$ and 100 ng/ml, respectively. To find detection limits for zoospores of P. katsurae, each zoospore suspension was serially diluted 10-fold to make the final concentrations from $1{\times}10^6$ to $1{\times}10^2$ cells/ml, and then DNA was extracted. The limits of detection for all of two primer sets were $1{\times}10^5$ cells/ml. All of two primer sets were specific to P. katsurae in PCR detection and did not produce any P. katsurae-specific PCR amplicons from other 16 Phytophthora species used as the control. This study shows that duplex PCR using two primer sets might be a useful tool for rapid and efficient detection of P. katsurae.

• Proceedings of the IEEK Conference
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• 2006.06a
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• pp.687-688
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• 2006

This paper presents implementation of the lifting based DWT processor interface which the process of JPEG2000. The proposed architecture uses Excalibur device produced Altera. This study describes CIS(CMOS Image Sensor), DMA(Direct Memory Access) and DWT control logic

• Journal of the Institute of Convergence Signal Processing
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• v.12 no.1
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• pp.13-19
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• 2011

In this paper, a visual surveillance system using SOPC based NIOS II embedded processor and C2H compiler was implemented. In this system, the IP is constructed by C2H compiler for the output of the camera images, image processing, serial communication and network communication, then, it is implemented to effectively control each IP based on the SOPC and the NIOS II embedded processor. And, an algorithm which updates the background images for high speed and robust detection of the moving objects is proposed using the Adaptive Gaussian Mixture Model(AGMM). In results, it can detecte the moving objects(pedestrians and vehicles) under day-time and night-time. It is confirmed that the proposed AGMM algorithm has better performance than the Adaptive Threshold Method(ATM) and the Gaussian Mixture Model(GMM) from our experiments.