• Title/Summary/Keyword: SEX IDENTIFICATION

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A Study on Care-Oriented Morality and Justice-Oriented Morality in Middle-Aged Adults (중년기의 도덕성에 관한 연구 - 보살핌 지향의 도덕성과 정의 지향의 도덕성을 중심으로 -)

  • 정옥분;곽경화
    • Journal of Families and Better Life
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    • v.20 no.3
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    • pp.13-25
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    • 2002
  • This is a study of care-oriented morality and justice-oriented morality among middle-aged adults. Thirty seven males and forty six females between the ages of 45 and 59 years old participated in this study Care-oriented morality was measured by the Ethic of Care Interview(ECI) while the justice-oriented morality was assessed using the Korean Defining Issues Test(DIT). Sex role identification was interpreted by using the Korean Sex Role Inventory(KSRI). In terms of care-oriented morality, the majority of the subjects were at level 2. The P(%) score of justice-oriented morality was found to be a little low. We found a significant difference between genders in terms of care-oriented morality, with females exhibiting a higher care-oriented morality. However, there was no significant difference in sex role identity types in terms of care-oriented morality. Nor did we find a significant interaction effect between gender and sex role identity. There was no gender difference in justice-oriented morality, but there was a significant difference among sex role identity types. Masculine males Possess the highest justice-oriented morality. A significant interaction effect was found to exist between sex role identity and gender.

A Relationship among Sexual contact, Sex Role Identity, and Self Esteem of Girls' High School Students (여고생의 성 접촉과 성역할 정체감 및 자아존중감에 대한 연구)

  • Sohn, Jung-Nam
    • Research in Community and Public Health Nursing
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    • v.14 no.3
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    • pp.468-478
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    • 2003
  • Purpose: The purpose of this study is to identify the relationship among sexual contact, sex role identification, and self esteem in Korean girls' high school students. Methods: The data were collected from 522 girls, who were stratified samples from a target population of 63,375 11th grade students from 200 regular high schools and 70 vocational high schools in Seoul, Korea from June 22 to July 18, 2002. Data were processed with the SAS program. Results: It was found that 42.51% of girls haven't experienced any sexual contact at all. The highest frequency in the final level of sexual contact was 'kiss', which was 22.03%. The 'androgyny' type among sex role identities of girls was most common, 32.57%, being followed by the 'undifferentiated' type 31.61%, the 'masculinity' type 18.97%, and the 'femininity' type 16.86, in that order. The sexual contacts such as 'holding hands', 'arms around', 'embracing', and 'kiss' were significantly different depending on sex role identity types. The score for self-esteem was significantly different depending on sex role identity types. The score for self- esteem was not significantly different depending on the final level of sexual contact. Conclusion: Based on the findings of this study, sex education programs should be developed in order to delay the sexual contacts of girls.

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Identification of Sex-Specific DNA Sequences in the Chicken (닭의 성특이적 DNA 분리)

  • Song, K.D.;Shin, Y.S.;Han, Jae Y.
    • Korean Journal of Poultry Science
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    • v.20 no.4
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    • pp.177-188
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    • 1993
  • This study was performed to find out the reasonable sexing methods In the chicken, obtain the basic information for the mechanisms related to chicken sexual differentiation and identify the genes which known to involved in chicken sex differentiation. The chromosome analysis of chicken embryonic fibroblast was a simple method to determine sex of chicken by means of Z and W chromosome identification. The bands of female chicken genomic DNA digested with Xho Ⅰ and Eco RI restriction endonuclease showed to be useful in direct sex determination and these repetitive sequences of Xho Ⅰ and Eco RI families were proposed to be very homologous in their sequences by colony hybridization analysis. Seven of 150 random primers were selected to amplify the W chromosome-specific band by using arbitrary primed PCR and three of them were useful to identify the sex of chicken. To identify the sex differentiation genes in the chicken, PCR for the amplification of ZFY and SRY sequences was performed. ZFY and SRY sequences were amplified successfully in the chicken genome, implying that chicken genome might have the sex-related conserved sequences similar to mammalian ones. The PCR products of ZFY amplification were the same in both sexes, suggesting that these sequences may be located on autosome or Z chromosome. The profile of PCR amplification for SRY sequences showed variation between sexes, but this result was not enough to specify whether the SRY gene in chicken is on the autosome or sex chromosome.

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MALDI Mass Spectrometric Analysis of Nonderivatized Steroids Using Cyclodextrin-supported 2,5-Dihydroxybenzoic Acid as Matrix

  • Son, Jeongjin;Cha, Sangwon
    • Bulletin of the Korean Chemical Society
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    • v.35 no.5
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    • pp.1409-1412
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    • 2014
  • Sex hormones are important metabolites in vertebrates' development and reproduction. For rapid screening sex hormones, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is one of the promising analytical platforms, but MALDI MS faces many challenges in detecting steroids such as low ionization efficiency and matrix background interference. One potential strategy to overcome matrix interference in the low m/z region is using a cyclodextrin (CD)-supported matrix for steroid analysis since CD-supported matrixes are known to effectively suppress matrix-related ion signals. In this study, we aimed to find the optimal CD-supported matrix for the analysis of the nonderivatized sex steroids. Our results showed that the ${\alpha}CD$-supported 2,5-dihydroxybenzoic acid (DHB) matrix efficiently ionized all three major classes of sex hormones, estrogens, androgens, and progestagens, with low or no matrix background and also with high sensitivity. In addition, the ${\alpha}CD$-supported DHB matrix mainly generated molecular ions or protonated ions of sex hormones, and this enabled us to obtain information-rich tandem mass spectra which potentially lead to unambiguous identification of steroid species from complex metabolite mixtures.

Identification of Sperm mRNA Biomarkers Associated with Sex-Determination in Korean Native Cows

  • Min, Kwan-Sik;Byambaragchaa, Munkhzaya;Kim, Hyun;Park, Myung-Hum
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.2
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    • pp.111-116
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    • 2019
  • This study was conducted to analyze the specific genes associated with sex-determination in Korean native cow. The highly organized spermatogenesis requires accurate spatial and temporal regulation of gene expression, which is governed by transcriptional, post-transcriptional, and epigenetic processes. Recently, farmers have been interested in determining the sexual identity of the calves in their farm. We analyzed the sperm of Korean native and Holstein cows, which were supplied from Hanwoo Improvement Center. We evaluated sperm motility and expression of sperm-specific genes after treating semen with both male- and female reagents. Sperm motility in Korean native cows decreased by approximately 10% in the first 30 minutes after treatment with sex-determination reagent. However, sperm motility of Holstein cows decreased to 60-70% after 15 minutes and to 20-30% after 30 minutes. We selected six specific genes expressing in the spermatozoa to analysis the gene expression level. The Real-time PCR results suggest that the selected genes (Gimap4, Tmeff1, Rac2, Abi2, Rac1, and Clu) were highly expressed in the group treated with the male reagent compared to the group treated the female reagent and to the untreated-group (control). In the present study, we suggest that the selected genes play a pivotal role in sex-determination.

The Study of Human Body Expression and Fashion Design Appeared in Popular Culture -Focused on Movies in Korea and American- (대중문화에서 보여지는 신체표현과 복식디자인 연구 - 미국과 국내 영화를 중심으로 -)

  • 권기영
    • The Research Journal of the Costume Culture
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    • v.12 no.4
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    • pp.676-690
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    • 2004
  • The purpose of this study is to investigate the symbolic meaning in body image and fashion style portrayed in Korea and American movies in aspects of sex, race, nature, and technology. The researcher analyzed movies in Korea and USA, and the results were as follows. In aspects of sex, movies portrayed new masculinity and femininity escaped from previous gender stereotypes, and the clothing styles characterized sex identity and sex roles. In aspects of race, more various races appeared in America movies than Korea's, and the appearance and stereotypes related with race were blurred by the globalization and the change of film market environments. In aspects of nature, Korea movie portrayed the human being as lyrical appearance in nature, and America movie expressed the human as a strong men conquering the nature in adventure movie or super human image combined with nature thing which endowed nature's superior characteristics to human body in fantasy and science fiction movies. In aspects of technology, the human bodies were described as cyborg, alien, or super human, which symbolized the do-identification in digital world, de-boundarization between human and machines, and the human's uncertainty.

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Sex Identification of Bovine Meat Using Male Specific SRY and ZFY Genes (웅성 특이적 SRY 및 ZFY 유전자를 이용한 쇠고기 성(性) 판별)

  • Shin, Sung-Chul;Chung, Ku-Young;Chung, Eui-Ryong
    • Food Science of Animal Resources
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    • v.27 no.3
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    • pp.351-356
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    • 2007
  • The objective of this study was to develop a rapid and reliable method for the sex determination of beef using the PCR(polymerase chain reaction) technique. We have used two bovine sex determining genes, SRY and ZFY, on the Y-chromosome to identify the sex of Hanwoo and Holstein beet. We attempted to amplify 1,348 bp and 979 bp fragments from male and female genomic DNA corresponding to the SRY and ZFY genes, respectively, using male specific primers. The amplified PCR products were separated by electrophoresis in a 1.5% agarose gel to detect a male specific DNA band. When DNA from male beef was amplified with primers specific for the SRY gene, a DNA band of 1,348 bp was present in all of the male samples, but absent from all of the female samples. Also, when DNA from male beef was amplified with primers specific for the ZFY gene, a DNA band of 979 bp was observed in all of the male samples, but absent from all female samples. In conclusion, the bovine SRY and ZFY genes are typically found only in male beef. For the practical application of this method for the sexing of commercial beef at the processing and marketing stages after slaughter. a total of 350 beef samples collected randomly from local markets were analyzed for sex determination. The proportions of male and female samples were 252 (72%) and 98 (28%), respectively. Therefore. the SRY and ZFY genes. which are specific for the Y-chromosome, may be useful sex-diagnostic DNA markers to distinguish male meat from female meat.

Identification of RAPD markers linked to sex determination in guggal [Commiphora wightii (Arnott.)] Bhandari

  • Samantaray, Sanghamitra;Geetha, K.A.;Hidayath, K.P.;Maiti, Satyabrata
    • Plant Biotechnology Reports
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    • v.4 no.1
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    • pp.95-99
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    • 2010
  • Decamer RAPD primers were tested on dioeceious and hermaphrodite plants of Commiphora wightii to identify sex-specific molecular markers. Sixty different random decamer primers were screened out of which only three primers were found to be associated with sex expression. A ~1,280-bp fragment from the primer OPN06 was found to be present in all the female individuals. Another primer OPN 16 produced a unique ~400-bp amplification product in only hermaphrodite individuals. The third marker, OPA20 amplified a ~1,140-bp fragment from female and hermaphrodite DNAs, but failed to do so from the male plant DNAs.

Morphometric analysis of the inter-mastoid triangle for sex determination: Application of statistical shape analysis

  • Sobhani, Farshad;Salemi, Fatemeh;Miresmaeili, Amirfarhang;Farhadian, Maryam
    • Imaging Science in Dentistry
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    • v.51 no.2
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    • pp.167-174
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    • 2021
  • Purpose: Sex determination can be done by morphological analysis of different parts of the body. The mastoid region, with its anatomical location at the skull base, is ideal for sex identification. Statistical shape analysis provides a simultaneous comparison of geometric information on different shapes in terms of size and shape features. This study aimed to investigate the geometric morphometry of the inter-mastoid triangle as a tool for sex determination in the Iranian population. Materials and Methods: The coordinates of 5 landmarks on the mastoid process on the 80 cone-beam computed tomographic images(from individuals aged 17-70 years, 52.5% female) were registered and digitalized. The Cartesian x-y coordinates were acquired for all landmarks, and the shape information was extracted from the principal component scores of generalized Procrustes fit. The t-test was used to compare centroid size. Cross-validated discriminant analysis was used for sex determination. The significance level for all tests was set at 0.05. Results: There was a significant difference in the mastoid size and shape between males and females(P<0.05). The first 2 components of the Procrustes shape coordinates explained 91.3% of the shape variation between the sexes. The accuracy of the discriminant model for sex determination was 88.8%. Conclusion: The application of morphometric geometric techniques will significantly impact forensic studies by providing a comprehensive analysis of differences in biological forms. The results demonstrated that statistical shape analysis can be used as a powerful tool for sex determination based on a morphometric analysis of the inter-mastoid triangle.