Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of RAPD markers linked to sex determination in guggal [Commiphora wightii (Arnott.)] Bhandari

  • Samantaray, Sanghamitra (Directorate of Medicinal and Aromatic Plants Research (DMAPR)) ;
  • Geetha, K.A. (Directorate of Medicinal and Aromatic Plants Research (DMAPR)) ;
  • Hidayath, K.P. (Directorate of Medicinal and Aromatic Plants Research (DMAPR)) ;
  • Maiti, Satyabrata (Directorate of Medicinal and Aromatic Plants Research (DMAPR))
  • Received : 2009.05.13
  • Accepted : 2009.09.03
  • Published : 2010.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Decamer RAPD primers were tested on dioeceious and hermaphrodite plants of Commiphora wightii to identify sex-specific molecular markers. Sixty different random decamer primers were screened out of which only three primers were found to be associated with sex expression. A ~1,280-bp fragment from the primer OPN06 was found to be present in all the female individuals. Another primer OPN 16 produced a unique ~400-bp amplification product in only hermaphrodite individuals. The third marker, OPA20 amplified a ~1,140-bp fragment from female and hermaphrodite DNAs, but failed to do so from the male plant DNAs.

Keywords

References

  1. Agrawal V, Sharma K, Gupta S, Kumar R, Prasad M (2007) Identification of sex in Simmondsia chinensis (Jojoba) using RAPD markers. Plant Biotech Rep 1:207-210 https://doi.org/10.1007/s11816-007-0031-6
  2. Ainsworth C, Crossley S, Buchanan-Wollaston V, Thangavelu M, Parker J (1995) Male and female flowers of the dioecious plant sorrel show different patterns of MADS box gene expression. Plant Cell 7:1583-1598 https://doi.org/10.1105/tpc.7.10.1583
  3. Alstrom-Rapaport C, Lascoux M, Wang YC, Roberts G, Tuskan G (1998) Identification of RAPD marker linked to sex determination in the basket willow (Salix viminalis L.). J Hered 89:44-49 https://doi.org/10.1093/jhered/89.1.44
  4. Atal CK, Gupta OP, Abag SH (1975) Commiphora mukul: source of medicine. Econ Bot 29:208-218
  5. Banerjee NS, Manoj P, Das MR (1999) Male sex associated RAPD markers in Piper longum L. Curr Sci 77:693-695
  6. Billore KV (1989) Some threatened medicinal plants of Rajasthan and their conservation. Indian For 115:595-599
  7. Bracale M, Caporali E, Galli MG, Longo C, Marziani-Longo G, Tassi F (1991) Sex expression and determination in Asparagus officinalis. Plant Sci 80:67-77 https://doi.org/10.1016/0168-9452(91)90273-B
  8. Claudete RF, Fairbanks DJ, Evans RP, Stutz HC, Andersen RW, Ruas PM (1998) Male-specific DNA in the dioecious species Atriplex garrettii (Chenopodiaceae). Am J Bot 85:162-167 https://doi.org/10.2307/2446304
  9. Dalal KC, Patel MA (1995) Guggal. In: Chadha KL, Gupta R (eds) Advances in horticulture: medicinal and aromatic plants. Malhotra, New Delhi, pp 491-501
  10. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from freshtissue. Focus 12:13-15
  11. Gangopadhaya G, Roy SK, Ghose K, Poddar R, Bandyopadhyay T, Basu D, Mukherjee KK (2007) Sex detection of Carica papaya and Cycas circinalis in pre-flowering stage by ISSR and RAPD. Curr Sci 92(4):524-526
  12. George J, Karun A, Manimekalai R, Rajesh MK, Remya P (2007) Identification of RAPD markers linked to sex determination in palmyrah (Borassus flabellifer L.). Curr Sci 93(8):1075-1077
  13. Hormaza JI, Dollo L, Polito VS (1994) Identification of a RAPD marker linked to sex determination in Pistacia vera using bulked segregant analysis. Theor Appl Genet 89:9-13
  14. Kafkas S, Cetiner S, Perl-Treves R (2001) Development of sexassociated RAPD markers in wild Pistacia species. J Hort Sci Biotechnol 76:242-246 https://doi.org/10.1080/14620316.2001.11511358
  15. Kakrani HK (1981) Guggul-a review. Indian Drugs 9:417-421
  16. Kapoor NK, Sukh Dev, Nityanand S (1979) Process for obtaining hypolipidemic and antiplatelet fractions from guggul resin. Indian patent no. 148265 dt. 6-4-1979
  17. Khadka DK, Nejidat A, Tal M, Golan-Goldhirsh A (2002) DNA marker for sex: molecular evidence for gender dimorphism in dioecious Mercurialis annua L. Mol Breeding 9:251-257 https://doi.org/10.1023/A:1020361424758
  18. Kumar S, Shankar V (1982) Medicinal plants of the Indian desert: Commiphora wightii (Arnott) Bhand. Arid Environ 5:1-11
  19. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistant genes by bulked segregant analysis: a rapid method to detect markers in specific genome regions using segregating populations. Proc Natl Acad Sci USA 88:9828-9832 https://doi.org/10.1073/pnas.88.21.9828
  20. Ming R, Moore PH (2007) Genomics of sex chromosomes. Curr Opin Plant Biol 10:123-130 https://doi.org/10.1016/j.pbi.2007.01.013
  21. Mondolino G, Carboni A, Forapani S, Faeti V, Ranali P (1999) Identification of DNA markers linked to male sex in dioecious hemp (Cannabis sativa L.). Theor Appl Genet 98:86-92 https://doi.org/10.1007/s001220051043
  22. Mulcahy DL, Weeden NF, Kesseli RV (1992) DNA probes for the Y-chromosome of Silene latifolia, a dioecious angiosperm. Sex Plant Reprod 5:86-88 https://doi.org/10.1007/BF00714562
  23. Nityanand S, Kapoor NK (1971) Hypocholesteremic effects of Commiphora mukul resin. Indian J Exp Biol 9:376
  24. Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985-993
  25. Parrish TL, Koelewijn HP, VanDijk PJ (2004) Identification of a male-specific AFLP marker in a functionally dioecious fig, Ficus fulwa Reinw. Ex Bl. (Moraceae). Sex Plant Reprod 17:17-22 https://doi.org/10.1007/s00497-004-0208-x
  26. Rao KSS, Patel DH, Dalal KC (1984) Occurrence of Commiphora stocksiana (Mitha Guggal) in Kutch. Indian Drugs 21:541-543
  27. Samantaray S, Hidayath KP, Maiti S (2009) An isolation protocol of genomic DNA from Commiphora wightii (Arnott.) Bhandari: an endangered medicinal plant. Int J Integr Biol 6:127-131
  28. Schlotterer C (2004) The evolution of molecular markers-just a matter of fashion? Nat Rev Genet 5:63-69 https://doi.org/10.1038/nrg1249
  29. Singh M, Kumar S, Singh AK, Ram D, Kalloo G (2002) Female sexassociated RAPD marker in pointed gourd (Trichosanthes dioica Roxb.). Curr Sci 82(2):131-132
  30. Taj-ud-din Krishna A, Dhuhan SPS, Ram D, Thakur RS (1994) Indian bdellium, Commiphora wightii. CROMAP 16:75-86
  31. Tanurdzic M, Banks JA (2004) Sex-determining mechanisms in land plants. Plant Cell 16:S61-S71 https://doi.org/10.1105/tpc.016667
  32. Urasaki N, Tokumoto M, Torora K, Ban Y, Kayano T, Tanaka N, Oku H, Chinen I, Terauchi R (2002) A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.). Theor Appl Genet 104:281-285 https://doi.org/10.1007/s001220100693
  33. Westergaard M (1958) The mechanism of sex determination in dioecious flowering plants. Adv Genet 9:217-281 https://doi.org/10.1016/S0065-2660(08)60163-7
  34. Williams JGK, Kubelik AR, Livak KJ, Rfalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531-6535 https://doi.org/10.1093/nar/18.22.6531
  35. Xu W, Wang B, Cui K (2004) RAPD and SCAR marker linked to sex determination in Euccommmia ulmoides Oliv. Euphytica 136:233-238 https://doi.org/10.1023/B:EUPH.0000032741.99885.c4

Cited by

  1. Identification of RAPD markers linked to nut weight and plant stature in cashew vol.129, pp.4, 2010, https://doi.org/10.1016/j.scienta.2011.05.006
  2. Hormonal control of sex expression of cucumber (Cucumis sativus L.) with the identification of sex linked molecular marker vol.55, pp.2, 2012, https://doi.org/10.1007/s13237-012-0061-5
  3. Identification of sex-specific DNA markers in betel vine (Piper betle L.) vol.59, pp.5, 2010, https://doi.org/10.1007/s10722-011-9707-4
  4. Problems, progress and future prospects of improvement of Commiphora wightii (Arn.) Bhandari, an endangered herbal magic, through modern biotechnological tools: a review vol.59, pp.6, 2010, https://doi.org/10.1007/s10722-012-9854-2
  5. Study of Flowering Behavior and Sex Determination in Garcinia indica (Thomas-Du Pettite) Choisy by Means of Molecular Markers vol.11, pp.4, 2010, https://doi.org/10.3923/biotech.2012.232.237
  6. <i>Commiphora wightii</i> (Arnott) Bhandari—A Natural Source of Guggulsterone: Facing a High Risk of Extinction in Its Natural Habitat vol.4, pp.6, 2010, https://doi.org/10.4236/ajps.2013.46a009
  7. Genetics of dioecy and causal sex chromosomes in plants vol.93, pp.1, 2010, https://doi.org/10.1007/s12041-014-0326-7
  8. Identification and Validation of a New Male Sex-Specific ISSR Marker in Pointed Gourd ( Trichosanthes dioica Roxb.) vol.2014, pp.None, 2010, https://doi.org/10.1155/2014/216896
  9. SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha vol.2016, pp.None, 2016, https://doi.org/10.1155/2016/1482796
  10. Identification of molecular markers associated with sex in kartoli (Momordica dioica Roxb.) vol.1241, pp.None, 2010, https://doi.org/10.17660/actahortic.2019.1241.40
  11. Phylogenic position and marker studies using cpDNA of C. wightii: A critically endangered and medicinally important plant in India vol.34, pp.2, 2010, https://doi.org/10.1007/s42535-021-00199-w