• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.1
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• pp.67-75
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• 2022

Roots play important roles in water and nutrient uptake and in response to various environmental stresses. Investigating diversification of cultivars through root phenotyping is important for crop improvement in adzuki beans. Therefore, we analyzed the morphological and architectural root traits of 22 adzuki bean cultivars using 2-dimensional (2D) root imaging. Plants were grown in plastic tubes [6 cm (diameter) × 40 cm (height)] in a greenhouse from July 25^th to August 28^th. When the plants reached the 2^nd or 3^rd trifoliate leaf stage, the roots were removed and washed with tap water to remove soil particles. Clean root samples were scanned, and the scanned images were analyzed using the WinRHIZO Pro software. The cultivars were analyzed based on six root phenotypes [total root length (TRL), surface area (SA), average diameter (AD), and number of tips (NT) were included as root morphological traits (RMT); and link average length (LAL) and link average diameter (LAD) were included as root architectural traits (RAT)]. According to the analysis of variance (ANOVA), a significant difference was observed between the cultivars for all root morphological traits. Distribution analysis demonstrated that all root traits except LAL followed a normally distributed curve. In the correlation test, the most important morphological trait, TRL, showed a strong positive correlation with SA (r = 0.97^***) and NT (r = 0.94^***). In comparison, between RMT and RAT, TRL showed a significantly negative correlation with LAL (r = -0.50^***); however, TRL did not show a correlation with LAD. Based on RMT and RAT, we identified the cultivars that ranked 5% from the top and bottom. In particular, the cultivar "IT 236657" showed the highest TRL, SA, and NT, while the cultivar "IT 236169" showed the lowest values for TRL, SA, and NT. In addition, the coefficient of variance for the six tested root traits ranged from (14.26-40%) which suggested statistical variability in root phenotypes among the 22 adzuki bean varieties. Thus, this study will help to select target root traits for the adzuki bean breeding program in the future, generating climate-resilient adzuki beans, especially for drought stress, and may be useful for developing biotic and abiotic stress-tolerant cultivars based on better root trait attributes.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.4
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• pp.280-291
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• 2007

Much research has been conducted in the field of phytoremediation since the discovery of the range of plants known as hyperaccumulators. Research has focused simultaneously on elucidating the mechanism of metal(loid) accumulation and development of practical techniques to enhance accumulation efficiency. To date, it is generally understood that there are five specific mechanisms employed by hyperaccumulating plant species that are either not or under utilized by non-hyperaccumulators. These include 1) enhanced metal(loid)s uptake through the root cell, 2) enhanced translocation in plant tissue, 3) detoxification and sequestration, 4) enhanced metal availability in soil:root interface, and 5) active root foraging toward metal(loid) enriched soils. Among these mechanisms, understanding of the plant-root effect on metal(loid) dynamics and subsequent plant uptake is vital to overcome the inherit limitation of phytoremediation caused by low metal(loid) solubility in soils. Plant roots can influence the soil chemistry in the rhizosphere through changes in pH and exudation of organic compounds such as low-molecular-weight organic acids (LMWOAs) which consequently change metal(loid) solubility. The decrease in soil pH by plant release of $H^+$ results in increased metal solubility. Elevated levels of organic compounds in response to high metal soil concentrations by plant exudation may also increases metal concentration in soil solution through formation of organometallic complexes.

• Korean Journal of Organic Agriculture
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• v.11 no.2
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• pp.103-113
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• 2003

The chemical properties of oak tree wood vinegar and the effect of wood vinegar on the tomato seedling were investigated to apply wood vinegar efficiently to the organic - and natural farming system. On the basis of the results from chemical properties of the oak tree wood vinegar, mineral nutrient contents of wood vinegar was low. Therefore, wood vinegar could not be a suitable nutrient source for the plant growth at 500∼1000 times dilution level. which commonly used in the farming, if only wood vinegar is supplied for the nutrient source for the plant growth. The application of wood vinegar increased root growth up the 500 times dilution level while decreased shoot growth. Furthermore. the anion concentrations such as nitrate and phosphate of the plant were decreased by the application of wood vinegar while cation concentrations such as K. Ca. and Mg were increased. Phenolic compounds of wood vinegar such as chlorogenic acid and ferulic acid enhanced the root growth. Interestingly the application of ferulic acid increased both root and shoot growth at the level of 10$^{-4}$ M concentration. It indicated that the effect of wood vinegar on the production of healthy plant seedling may be due to the beneficial root growth by phenolic compounds such as chlorogenic acid and/or ferulic acid of the wood vinegar. However. the effect of the wood vinegar on the plant growth could be influenced by synergism or antagonism of different phenolic compounds in wood vinegar used. In addition. drench in the soil of wood vinegar may be more beneficial compared to foliar application for the improvement of root activity and plant growth.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.31-37
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• 2000

Proton extrusion by hairy roots of Panax ginseng C.A. Meyer was enhanced by iron-deficiency or single salt solutions of nitrate or ammonium salts. The enhancement effect of iron-deficiency was confined to the apical zones, whereas that of nitrate and ammonium was confined to the basal root zones. tinder iron-deficiency medium, the proton extrusion of ginseng hairy roots was higher at two times than that grown in the medium of iron-sufficiency. According to pH in the medium, uptake of inorganic elements showed various patterns for the kind of inorganic elements. $Ca^{2＋}$, $Mg^{2＋}$ and Fe$^{2＋}$ were highest uptake at the pH 4.8 and Mn$^{2＋}$, Zn$^{2＋}$ and Cu$^{2＋}$ were highest uptake at the pH 3.8. Free sugars and ginsenosides were analyzed by HPLC. Five kinds of free sugars were indentified in ginseng hairy roots. Glucose, fructose and sucrose were a major sugar in ginseng hairy roots and contents of free sugars were highest at pH 6.8 and pH 4.8, respectively. The contents of ginsenosides and phenolic compounds were highest at pH 5.8. This physiological responses in ginseng hairy root indicated that it may well be a good model system for the study of mechanism of mobilization and uptake of inorganic elements related with the red-colored phenomenon in ginseng roots.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.2
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• pp.178-183
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• 1997

The effect of different relative humidity(RH) of atmosphere on the uptake of calcium by tomato plant was investigated through an experiment in the protected cultivation system. The RH regime was imposed by humidifiying by humidifier in one plot, and by mulching the ridge to cut down the evaporation of water to lower the RH, in another plot. During the course of plant growth, RH in the humidified plot, at noon, was about 70%, while in non-humidufied plot, RH was about 50%. The humidification also resulted in the lowering of temperature significantly(by about, $3.1^{\circ}C$). This higher RH of atmosphere under humidifying treatment, resulted in the increase in the water efflux rate of root significantly(greater by 0.24g/g dry root/h than that under mulching treatment). Relatively severe occurrence of blossom end rot(23%) was observed in the humidifying treatment, while no such symptom occurred in mulching plot. The efflux rates of Ca, K and Mg were found to be higher in the humidifying plot. It was also observed that the concentrations of Ca, Mg and K in the xylem solution was 2-4 times higher than that of gydroponic solution. This suggested that the occurrence of blossom end rot, under high RH of atmosphere, would not be due to the decrease in the uptake of Ca per se, by tomato plant, but due to anomalies in the distribution of it within the plant.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.2
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• pp.103-110
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• 2009

Rape plants, especially forage type, are known as one of high S-demanding plants. Their productivity and quality have often limited under S-deficient condition. To investigate the effect of S-deficiency on N uptake and its assimilation, $NO_3^-$ absorption, nitrate reducatse (NR) and glutamine synthetase (GS) activity in leaf and root tissues as affected by different S-supplied level was determined. $NO_3^-$ uptake was not significant between control and S-deficient treated plants, while significantly depressed in S-deprived plants for the early 8 h. NR activity decreased as S-availability decreased, especially in young and middle leaves, representing more than 35% of decrease in S-deficient and 70% in S-deprived plants when compared with control. In roots, a significant decrease (-29%) in NR was observed only in S-deprived plants. Relatively higher GS activity was found in young leaves for three all treatments. As a whole leaf tissue, S-limited conditions resulted in a reduction of GS activity. In root which showed the lowest activity, a significant decrease (-30%) was observed only in S-deprived plants.

• Korean Journal of Soil Science and Fertilizer
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• v.13 no.4
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• pp.115-118
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• 1981

Effect of water potential of culture solution on photosynthesis, transpiration and water uptake was investigated using polyethylene glycol 6000. 1. Even at -0.5 bar of culture solution phothosynthesis was decreased by 20% within 1 hour. Plant in control showed 3.26% loss of initial water for 13 hours suggesting very sensitive in water uptake. 2. Relation between water potential of culture solution (${\psi}$) and water uptake amount (W) 2-year root was ${\psi}=-2.890/e^{2.796W}$ indicating that permanent wilting point will be greater than -2.89 bar. 3. Transpiration considerably decreased with the decrease of water potential and thus by 23.9% at -0.5 bar after 4 hours. 4. From the above results ginseng plant appears to have high root water potential at permanent wilting point and thus very week to water stress due to drought or high salt content in soils.

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.10b
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• pp.326-327
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• 2005

• Journal of Forest and Environmental Science
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• v.35 no.4
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• pp.240-247
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• 2019

This study quantified carbon reduction services through direct harvesting of Ilex rotunda and Machilus thunbergii, which are the typical urban landscape tree species in southern Korea. A total of 20 open-grown tree specimens (10 specimens for each species) were selected reflecting various sizes of stem diameter at breast height of 1.2 m (DBH) at a regular interval. The study measured biomass for each part of the tree specimens including roots to compute total carbon storage per tree. Annual carbon uptake per tree was also calculated analyzing the DBH growth rate of stem disk specimens. Quantitative models were developed using DBH as an independent variable to easily estimate storage and annual uptake of carbon by tree growth for each species. All the models had a high goodness-of-fit with R²=0.95-0.99. The difference in carbon reduction services between DBH sizes increased with increasing DBH. The storage and annual uptake of carbon from a tree with DBH of 10 cm were 13.5 kg and 2.4 kg/yr for I. rotunda, and 19.1 kg and 3.6 kg/yr for M. thunbergii, respectively. The tree of this size stored the amount of carbon equivalent to that emitted from a gasoline use of approximately 24 L for I. rotunda and 34 L for M. thunbergii, respectively. The study provides actual measurement data to quantify carbon reduction services of urban open-grown landscape trees for the warm-temperate species that have been little known until now.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.2
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• pp.118-122
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• 2006

This experiment was carried out on plastic pots ($40cm{\times}25cm{\times}30cm$) filled with sand soil at greenhouse using two soybean cultivars with small seed; one was Pungsannamulkong (PSNK) recognized as a tolerant cultivar against excessive water stress and the other one was Sobaeknamulkong (SBNK) recognized as a susceptible cultivar. Seed was sown with 30 plants of 2 hills, and the amount of applied fertilizer was N; 3.0 g, P; 3.0 g, and K; 3.4 g per $m^2$ with all basal fertilizations. Plants were grown under photoperiod of natural light with day temperature of $31{\pm}5^{\circ}C$ and night temperature of $22{\pm}1^{\circ}C$. The flooding treatment was done for 3, 5, 7 and 10 days by filling pots with tap water up to 1 cm above the level of the soil surface when plants were 2 days after emerging. Nitrogen uptake by leaves of soybeans decreased significantly by the flooding after 6 days. This significant reduction of N uptake by flooding was evidently recognized from the chlorosis of leaves. The dry matter of flooded soybean seedlings significantly decreased compared to non-flooded soybean seedlings at 10 days. The dry matter of roots also showed similar result of the shoot. Shoots had more N reduction than roots under the flooding. This N reduction was more pronounce in SBNK than in PSNK. Chlorophyll content of flooded soybeans showed decreasing or non-increasing tendency, and the reduction of chlorophyll content was more in SBNK than in PSNK from the flooding stress. Nitrate content of soybean seedlings with flooding stress showed decreasing tendency in shoot and root parts. Ammonium content, however, was higher in flooding stress compared to the non-flooding. Flooding caused a remarkable change in the AA (amino acid) composition and TAA (total amino acid) concentration in the leaves of soybean seedlings.