Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Sulfur Deficiency Effects on Nitrate Uptake and Assimilatory Enzyme Activities in Rape Plants

유채에서 황 결핍이 질산염의 흡수 및 동화관련 효소활력에 미치는 영향

  • Li, Lu-Shen (Chonnam National University) ;
  • Jin, Yu-Lan (BK21 Research Team for the Control of Animal Hazards using Biotechnology, College of Agriculture & Life Science, Chonnam National University) ;
  • Lee, Bok-Rye (BK21 Research Team for the Control of Animal Hazards using Biotechnology, College of Agriculture & Life Science, Chonnam National University) ;
  • Kim, Tae-Hwan (Chonnam National University)
  • Published : 2009.06.30
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Abstract

Rape plants, especially forage type, are known as one of high S-demanding plants. Their productivity and quality have often limited under S-deficient condition. To investigate the effect of S-deficiency on N uptake and its assimilation, $NO_3^-$ absorption, nitrate reducatse (NR) and glutamine synthetase (GS) activity in leaf and root tissues as affected by different S-supplied level was determined. $NO_3^-$ uptake was not significant between control and S-deficient treated plants, while significantly depressed in S-deprived plants for the early 8 h. NR activity decreased as S-availability decreased, especially in young and middle leaves, representing more than 35% of decrease in S-deficient and 70% in S-deprived plants when compared with control. In roots, a significant decrease (-29%) in NR was observed only in S-deprived plants. Relatively higher GS activity was found in young leaves for three all treatments. As a whole leaf tissue, S-limited conditions resulted in a reduction of GS activity. In root which showed the lowest activity, a significant decrease (-30%) was observed only in S-deprived plants.

유채(Brassica napus L.)에서 황 결핍이 $NO_3^-$ 흡수와 동화에 대한 영향을 알아보고자 $SO_4^{2-}$ 농도를 세가지 수준(1 mM $SO_4^{2-}$, 대조구; 0.1mM $SO_4^{2-}$, 결핍; 0 mM $SO_4^{2-}$, 무공급)으로 25시간 처리한 후 $NO_3^-$ 흡수량, 식물조직내의 nitrate reductase (NR) 및 glutamine syntheetase (GS) 활성을 분석하였다. 25시간 처리과정에서 황결핍 조건하에서의 $NO_3^-$의 흡수는 대조구와 큰 차이를 나타내지 않은 반면, 황 무공급구에서는 $NO_3^-$의 흡수는 유의적으로 감소하였다. NR 활성은 잎과 뿌리 조직에서 공히 $SO_4^{2-}$ 농도가 감소됨에 따라 감소하였다. 특히 어린잎과 중간 잎의 황 결핍 처리구에서 각각 35% 및 황 무공급구에서 70%의 뚜렸한 감소를 보였다. 뿌리에서는 오직 황 무공급구에서 유의적인 감소 (-29%)를 보였다. $SO_4^{2-}$ 농도에 따른 잎 조직에서 GS 활성은 잎의 성숙 정도에 따라 차이를 보였으며, 전반적으로 황 결핍과 황 무공급구에서 감소하는 경향을 보였다. 뿌리조직에서 GS 활성은 황 무공급구에서만 유의적인 감소를 보였다. 이러한 결과들은 황 공급이 제한을 받는 조건에서 질산염의 흡수가 감소되며, 식물조직체내의 질산염환원과 아미노산 합성관련 효소의 활성이 제한을 받았다는 것을 보여준다.

Keywords

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