• 한국염색가공학회:학술대회논문집
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• 한국염색가공학회 2011년도 제45차 학술발표회
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• pp.34-34
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• 2011

Recently, people have concerned about environmental pollution. This environmental pollution occur due to many reasons such as heavy metal ions and anions. In this regard, many researchers have studied organic materials to monitor above reasons to protect environmental pollution. One of the organic materials for this function is chemosensor. This chemosensor has been studied and reported about monitoring toxic heavy metal ions and anions. In this study, the dye sensor was designed and synthesized through reaction of Rhodamine 6G and 1,3-Indanedion. this dye sensor selective detected $Hg^{2+}$ metal ions while showing red color absorption and yellowish-green strong fluorescence emission compared to other heavy metal ions such as $Cu^{2+}$, $Hg^{2+}$, $Ag^{2+}$, $Zn^{2+}$, $Fe^{2+}$ and $Fe^{3+}$. In this regard, we anticipated that this dye senosr can provide an significant material for monitoring mercury which cause environmental pollution. Thus, We investigated detailed properties of this dye sesnor with using UV-Vis absorption and fluorescent spectrophotometer, Job's plot method for metal binding complex, computational simulated calculation named Material Studio 4.3 suite to approach for electron distribution and HOMO/LUMO.

• Ecology and Resilient Infrastructure
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• 제3권4호
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• pp.263-271
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• 2016

본 연구에서는 호소 수체에 산소를 포기시켜 주는 마이크로 버블 발생장치를 충남 아산에 위치하고 있는 죽산호에 설치하여 수체에 미치는 수리학적 영향반경과 용존산소 (DO) 농도 분포를 조사하였다. 수체에 미치는 수리학적 영향반경은 로다민 염료를 이용한 추적실험을 하였으며, 마이크로 버블 제트류의 수평방향으로 총 160 m 길이의 가이드라인을 설치하여 10 m 지점마다 수심 1 m, 2 m, 3 m에서 로다민 농도, 수온, DO 등을 측정하였다. 죽산호의 로다민 배경농도는 $0.3-0.5{\mu}g/L$이었고, 용액 주입 후 15분 이내에 20-40 m 거리까지 확산되었으며, 거리 50-120 m의 수심 2 m 지점에서 로다민 농도가 $3.1-12{\mu}g/L$를 나타냈다. 본 연구결과로부터 마이크로 버블 발생장치의 제트류에 의한 직접적인 영향 범위는 40-50 m이고, 그 이후에는 이류 및 확산에 의한 영향으로 120 m까지 이동하는 것으로 나타났다. 수중 DO 농도는 경과시간과 이격 거리에 상관없이 7.4-12.6 mg/L의 농도를 유지하였고, 마이크로 버블 적용 전의 호소 바닥층 DO 0.2 mg/L에서 운영 후 8.0 mg/L 이상으로 DO가 개선되는 것으로 나타났다. 본 연구 결과에 의하면 마이크로 버블 포기 기술은 농업용 호소의 수질 관리와 개선에 기여할 것으로 기대된다.

• 한국잡초학회지
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• 제18권4호
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• pp.304-313
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• 1998

유제(乳劑)를 살포하였을 때 벼와 피에 대한 경엽부착량을 다양한 조건에서 조사하고 그것을 약효 및 약해와 연관시켜 보았다. 경엽부착량의 측정은 형광물질인 rhodamine B를 이용하였으며 그 결과는 아래와 같았다. 1. 피에 대한 경엽부착량은 표준처리조건 (약량; 30g ai/ha, 처리물량; 1000 L/ha)에서 disk-type 노즐 (국내 수동배부식 분무기용)로 전착제 가용시, 비가용시, flat-fan 노즐 (구미 boom-sprayer용)로 전착제 가용시, 비가용시, 경엽 생체중 1g 당(當) 각각 2.3-2.7, 1.4-1.5, 0.6-0.7, $0.3-0.5{\mu}g$ 이 었다. 2 처리물량을 고정시키고 농도를 변화시킨 경우와 농도를 고정시키고 처리물량을 변화시킨 경우에 부착량 증감의 기울기는 각각 1.0-1.8, 1.6-2.4로 후자의 경우가 변동의 폭이 더 컸다. 3. 피에 대한 경엽부착량은 약효와 밀접한 연관이 있었으며, 90% 방제를 나타내는 부착량은 경엽 생체중 1g 당(當) 약 $0.8{\mu}g$이었고, 그 이하의 부착량에서는 약효가 거의 직선적으로 감소하였다. 4. 벼에 대한 경엽부착량은 표준처리조건에서 disk-type 노즐로 전착제 가용시, 비가용시, flat-fan 노즐로 전착제 가용시, 비가용시, 경엽 생체중 1g 당(當) 각각 1.9-2.3, 1.2-1.3, 0.6-0.9, $0.3{\mu}g$이었다. 5. 처리물량을 고정시키고 농도를 변화시킨 경우와 농도를 고정시키고 처리물량을 변화시킨 경우에 부착량 증감의 기울기는 각각 1.0-2.8, 1.3-4.4로 파에서와 마찬가지로 후자의 경우가 변동의 폭이 더 컸다. 6. 4배 이내의 약량(농도) 또는 살포량 증가 시에 벼에 대한 pyribenzoxim의 경엽부착량의 증가나 노즐, 전착제 유무에 의한 차이는 약해와 관련이 없었다. 7. 타(他)약제와 혼용시 벼에 대한 pyribenzoxim의 경엽부착량은 약제에 따라 약 30-50% 증가하는 경향이었다.

• 한국환경과학회지
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• 제19권9호
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• pp.1143-1152
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• 2010

The purpose of this study is to degradation of Rhodamine B (RhB, dye) and N, N-Dimethyl-4-nitrosoaniline (RNO, indicator of the electro-generation of OH radical) in solution using boron doped diamond (BDD) electrode. The effects of applied current (0.2~1.0 A), electrolyte type (NaCl, KCl, and $Na_2SO_4$) and electrolyte concentration (0.5~3.0 g/L), solution pH (3~11) and air flow rate (0~4 L/min) were evaluated. Experimental results showed that RhB and RNO removal tendencies appeared with the almost similar thing, except of current. Optimum current for RhB degradation was 0.6 A, however, RNO degradations was increased with increase of applied current. The RhB and RNO degradation of Cl type electrolyte were higher than that of the sulfate type. The RhB and RNO degradation were increased with increase of NaCl concentration and optimum NaCl dosage was 2.5 g/L. The RhB and RNO concentrations were not influenced by pH under pH 7. Optimum air flow rate for the oxidants generation and RhB and RNO degradation were 2 L/min. Initial removal rate of electrolysis process was expressed Langmuir - Hinshelwood equation, which is used to express the initial removal rate of UV/$TiO_$2 process.

• 한국분말재료학회지
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• 제21권6호
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• pp.441-446
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• 2014

In this study, we synthesize silica-core gold-satellite nanoparticles (SGNPs) for the surface-enhanced Raman scattering (SERS) based sensing applications. They consist of gold satellite nanoparticles (AuNPs) fixed on the silica core nanoparticles, which sizes of AuNPs can be tunned by varying the amount of reactants (growth solution and reducing agent). Their surface plasmon resonance (SPR) properties were characterized by using UV-vis spectroscopy, showing that the growth of AuNPs on silica cores leads to the light absorption in the longer wavelength region. Furthermore, the size increase of AuNPs exhibited the dramatic change in SERS activity due to the formation of hot spots. The optimized SGNPs showing enhancement factor ${\sim}3.8{\times}10^6$ exhibited a detection limit of rhodamine 6G (R6G) as low as $10^{-8}M$. These findings suggest the importance of size control of SGNPs and their SPR properties to develop highly efficient SERS sensors.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2015년도 추계학술대회 논문집
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• pp.251-251
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• 2015

최근 질병 조기진단에 대한 사회적 요구가 높아짐에 따라 이에 대한 기술에 관심이 집중되고 있다. 그 중 표면증강라만산란(surface enhanced Raman scattering(SERS))을 이용하여 인체 내 소량의 바이오마커를 검출하는 연구가 활발히 진행중이다. 본 연구에서는 바이오마커의 검지감도를 최대치로 증가시키기 위해 SERS 기판의 나노구조를 최적화 하였다. SERS 기판 표면의 나노구조, 크기, 형상, 밀도 등에 따라 검지감도가 변화되기 때문에 이를 제어하기 위해 증착공정 변수에 변화를 주어 표면의 나노구조를 형성하였다. 이를 분석하기 위해 SEM, XRD를 사용하였으며 최적화된 SERS 기판을 활용하여 Rhodamine 6G의 신호가 $1{\times}10^5$ 이상의 enhancing factor를 확인하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제15권6호
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• pp.353-361
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• 2011

In this study, cyanidin-3-glucoside (C3G) fraction extracted from the mulberry fruit (Morus alba L.) was investigated for its neuroprotective effects against oxygen-glucose deprivation (OGD) and glutamate-induced cell death in rat primary cortical neurons. Cell membrane damage and mitochondrial function were assessed by LDH release and MTT reduction assays, respectively. A time-course study of OGD-induced cell death of primary cortical neurons at 7 days in vitro (DIV) indicated that neuronal death was OGD duration-dependent. It was also demonstrated that OGD for 3.5 h resulted in approximately 50% cell death, as determined by the LDH release assay. Treatments with mulberry C3G fraction prevented membrane damage and preserved the mitochondrial function of the primary cortical neurons exposed to OGD for 3.5 h in a concentration-dependent manner. Glutamate-induced cell death was more pronounced in DIV-9 and DIV-11 cells than that in DIV-7 neurons, and an application of $50{\mu}M$ glutamate was shown to induce approximately 40% cell death in DIV-9 neurons. Interestingly, treatment with mulberry C3G fraction did not provide a protective effect against glutamate-induced cell death in primary cortical neurons. On the other hand, treatment with mulberry C3G fraction maintained the mitochondrial membrane potential (MMP) in primary cortical neurons exposed to OGD as assessed by the intensity of rhodamine-123 fluorescence. These results therefore suggest that the neuroprotective effects of mulberry C3G fraction are mediated by the maintenance of the MMP and mitochondrial function but not by attenuating glutamate-induced excitotoxicity in rat primary cortical neurons.

• 대한한의학방제학회지
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• 제15권1호
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• pp.213-228
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• 2007

Objectives : The purpose of this report was to investigate the chemotherapeutic effect of Bujeonghangamtang against cancer cells. Materials and Methods : Various cancer cell lines including PANC-1, C6 glioma, SH-SY5Y, HepG2, and MCF-7 cells, were used. Apoptosis was determined by DAPI nuclei staining and flow cytometry in PANC-1 cells treated with 1 mg/ml Bujeonghangamtang for 48 hr. Expression of cell cycle arrest mediators including, cdc2p34 and cyclin B1 proteins were measured by Western blot analysis. Mitochondrial membrane potential was measured by fluorescence staining with JC-1, rhodamine 123. Result : Bujeonghangamtang induced the apoptosis of PANC-1, which was characterized as nucleic acid and genomic DNA fragmentation, chromatin condensation, and sub-G0/G1 fraction of cell cycle increase. but not C6 glioma, SH-SY5Y, HepG2, and MCF-7 cells. PANC-1 cells were markedly sensitive to Bujeonghangamtang. Treatment with Bujeonghangamtang resulted in the decreased expression of cdc2p34 and cyclin B1. Treatment with Bujeonghangamtang also increased the ROS production and induced mitochondrial dysfunction. Conclusion : Bujeonghangamtang exerted cytotoxicity against human Pancreatic cancer cells via cell cycle arrest-mediated apoptotic signaling including ROS production and mitochondrial dysfunction. Our data suggest that Bujeonghangamtang may be an important modulator of chemosensitivity of cancer cells against anticancer chemotherapeutic agents.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1369-1376
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• 2006

Self-organized nanogels were prepared from pullulan/biotin conjugates (PU/Bio) for the development of an effective anticancer drug delivery system. The degree of biotin substitution was 11, 19, and 24 biotin groups per 100 anhydroglucose units of pullulan. The physicochemical properties of the nanogels (PU/Bio1, 2 and 3) in aqueous media were characterized by dynamic light scattering, transmission electron microscopy, and fluorescence spectroscopy. The mean diameter of all the samples was less than 300 nm with a unimodal size distribution. The critical aggregation concentrations (CACs) of the nanoparticles in distilled water were $2.8{\times}10^{-2},\;1.6{\times}10^{-2}$, and $0.7{\times}10^{-2}mg/ml$ for the PU/Bio1, 2, and 3, respectively. The aggregation behavior of the nanogels indicated that biotin can perform as a hydrophobic moiety. To observe the specific interaction with a hepatic carcinoma cell line (HepG2), the conjugates were labeled with rhodamine B isothiocyanate (RITC) and their intensities measured using a fluorescence microplate reader. The HepG2 cells treated with the fluorescence-labeled PU/Bio nanoparticles were strongly luminated compared with the control (pullulan). Confocal laser microscopy also confirmed internalization of the PU/Bio nanogels into the cancer cells. Such results demonstrated that the biotin in the conjugate acted as both a hydrophobic moiety for self-assembly and a tumor-targeting moiety for specific interaction with tumor cells. Consequently, PU/Bio nanogels would appear to be a useful drug carrier for the treatment of liver cancer.

• 한국물환경학회지
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• 제26권6호
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• pp.963-968
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• 2010

This study has carried out to evaluate the performance of direct and indirect oxidation electrode for the purpose of decolorization of Rhodamine B (RhB) in water. Four kinds of electrodes were used for comparison: Pt and JP202 (indirect oxidation electrode), Pb and boron doping diamond (BDD, direct oxidation electrode). The effect of applied current (0.5 ~ 2.5 A), electrolyte type (NaCl, KCl, HCl, $Na_2SO_4$ and $H_2SO_4$) and electrolyte concentration (0.5 ~ 2.5 g/L), solution pH (3 ~ 11) and initial RhB concentration (25 ~ 125 mg/L) were evaluated. Experimental results showed that RhB removal efficiency were increased with increase of current, NaCl dosage and decrease of the pH. However, the effect of operating parameter on the RhB removal were different with the electrode type. JP202 electrode was the best electrode from the point of view of performance and energy consumption. The order of removed RhB concentration per energy lie in: JP202>Pt>Pb>BDD.