• 한국잡초학회지
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• 제32권3호
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• pp.180-187
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• 2012

Panicum maximum, Panicum repens, Imperata cylindrica, Pennisetum polystachion and Cyperus rotundus are five rhizomatous grass weeds that exist and are problematic in the coconut lands of Sri Lanka. Rhizomatous weeds are the most difficult to control because of their vegetative reproduction by underground propagules. Therefore chemical and biological techniques have failed to control these weeds to acceptable levels. Experiments were conducted to investigate the impact of depth of burial, duration of sun drying or duration of air drying rhizomes of the selected weed species on germination. Depth of burial reduced germination of all species. Burying rhizomes at depths less than 10 cm inhibited germination of P. maximum and P. polystachion. Burying depths below 30~40 cm inhibited germination of all species. Sun drying or air drying rhizomes for durations less than 5 days inhibited germination of P. maximum and P. polystachion. Germinations of all species were inhibited by sun drying rhizomes for 15 days or air drying rhizomes for 20 days. Sun drying of rhizomes of all species for five days reduced the moisture content to a greater extent than air drying. The results indicated that burying rhizomes at the depthsbelow 30~40 cm, sun drying rhizomes for durations beyond 15 days or air drying rhizomes for durations beyond 20 days would be effective in controlling germination of these species. The investigations of the experiments also suggest that keeping rhizomes on the soil surface without burying, sun drying rhizomes or air drying rhizomes for durations of 5~15 days would produce weak plants.

• 대한본초학회지
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• 제34권5호
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• pp.39-47
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• 2019

Objectives : When herbal medicines are extracted together, they may interact with each other, leading to change of chemical characteristics. This study aimed to evaluate the influence of Atractylodes rhizomes (Atractylodes japonica, A. macrocephala, and A. chinensis) on the chemical features of the roots and rhizomes of Glycyrrhiza uralensis, which is are commonly combined with herbal medicines in many herbal formulae, when they are co-decocted. Methods : Liquiritin apioside, liquiritin, ononin, and glycyrrhizin levels of G. uralensis in hot-water extracts prepared by the combination of Atractylodes rhizomes with various weight ratios (G. uralensis : Atractylodes rhizomes = 10:0, 10:5, 10:10, and 10:20) and extraction times (60, 90, and 120 min) were quantified using a HPLC-diode array detector and compared by statistical analysis. Results : The concentrations of liquiritin apioside, liquiritin, ononin, and glycyrrhizin from G. uralensis roots and rhizomes mostly reduced when co-extracted with Atractylodes rhizomes, and the addition of A. chinensis most reduced their contents between Atractylodes combination groups. A. japonica and A. macrocephala rhizomes also showed differences of liquiritin and glycyrrhizin levels at 10 g and 20 g groups of Atractylodes rhizomes. Extraction times also affected the concentrations of liquiritin, ononin, and glycyrrhizin mostly during 60 and 90 min. Conclusions : Atractylodes rhizomes might alter the chemical characteristics of G. uralensis when these herbs are co-decocted. This study provides the understanding of the chemical interactions of herbal medicines during the extraction in hot water.

• 한국약용작물학회지
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• 제26권5호
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• pp.354-361
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• 2018

Background: This study was conducted to investigate the growth characteristics, root yield and curcuminoid content of turmeric (Curcuma longa L.), cultivated using mother seed rhizomes (MR) and finger seed rhizome (FR) of different sizes. MR are attached to the stem, and FR are connected to the MR, and are used as a general seed rhizome. Methods and Results: Seed rhizomes of different types and sizes were used: large, medium and small for FR, and large, half-sized, and small for MR. These were assigned to the experimental groups and cultivated under greenhouse conditions. The growth characteristics, root yield, and curcuminoid content did not show clear difference between MR and FR, but suggest that the larger seed rhizomes (above 30 g) could have higher root yields. On average, harvested mother rhizomes (HMR) contained more curcuminoid than harvested finger rhizomes (HFR), while the yield of HFR was higher than that of HMR. The higher weight of harvested roots correlated significantly with elevated curcuminoid content. Conclusions: The two seed rhizomes, MR and FR, did not differ in root yield and curcuminoid contents, but larger seed rhizomes may produce better root yields. This suggest that the optimum seed rhizome is larger FR, to produce higher yields and quality in turmeric root production.

• 대한본초학회지
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• 제31권5호
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• pp.25-39
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• 2016

Objectives : Atractylodes rhizomes, which have been widely used to treat gastrointestinal disorders, consist of numerous chemical compounds. Polyacetylenes are the parts of characteristic compounds of importance required to understand the therapeutic properties of Atractylodes rhizomes. It is necessary to understand the physicochemical and pharmacological properties of polyacetylenes in the Atractylodes rhizomes.Methods : The literatures from 1970 to January 2016 were searched using Korean and international electronic databases. The chemical structures of polyacetylenes were drawn by structure-drawing software.Results : The reported polyacetylenes were classified by their chemical skeletons and original resources, and their physicochemical and pharmacological features were discussed. Polyacetylenes with skeletal moieties were reported, such as diene-diyne types (two double and two triple carbon-bonds), triene-diyne types (three double carbon bonds and two triple carbon bonds), and monoene-diyne types (one double carbon bonds and two double carbon bonds), with various functional groups. Atractylodin was most frequently reported from many Atractylodes species. Atractylodin-related polyacetylenes showed chemical instability in both high and freezing temperatures. Processing of the Atractylodes rhizomes by stir-frying with bran could affect the contents of polyacetylenes and their bioavailability in vivo. Several polyacetylenes showed structure-related anti-inflammatory activities and gastrointestinal activities.Conclusion : Polyacetylene compounds in Atractylodes rhizomes were based on three chemical backbones and showed diverse physicochemical and pharmacological features. The present study provides structural, physicochemical, and pharmacological information of polyacetylene from Atractylodes rhizomes. This information provides fundamental data for further research.

• 한국약용작물학회지
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• 제28권1호
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• pp.47-55
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• 2020

Background: Curcuma longa L., a perennial crop originating from tropical and subtropical region, including India, is noted for its important medicinal properties. However, C. longa plants are unable to endure the winter season in Korea, and its rhizomes were invariably succumb to fungal infection when stored in polyvinyl bags. In this study, we accordingly sought to develop a C. longa variety capable of producing high rhizome yields and to identify stable conditions under which rhizomes can be stored in Korea. Methods and Results: We evaluated the agronomic characteristics of nine C. longa germplasms and examined the effects of storing rhizomes at different temperatures (4℃ to 24℃) in paper bags or plastic baskets. We found that the finger rhizomes was higher in CUR02, CUR03, and CUR06 germplasms than those of other groups. Furthermore, in terms of yield per 1 ㎡, the weights of the finger rhizomes and tuberous roots were significantly higher in CUR09 (3.4 ㎏/㎡) and CUR04 (678.7 g/㎡) than those of other groups. Therefore, we consider that these C. longa germplasms might be useful as breeding material. Although the fresh weights of the rhizomes were slightly reduced when stored in paper bags and a plastic baskets at 10℃ to 15℃, there was no evidence of fungal decomposition or sprouting, which is observed when using a conventional storage method. Conclusions: The results of this study indicate that the selected C. longa germplasms can provide a useful source of breeding material for the development of high yielding varieties and that a temperature ranging from 10℃ to 15℃ and the use of paper bags or plastic baskets provide stable post-harvest storage conditions for C. longa rhizomes.

• The Korean Journal of Ecology
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• 제24권2호
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• pp.81-85
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• 2001

Decomposition of Zizania latifolia was investigated with litterbag method for 13 months from November in 1998 to December in 1999, at the fringe of stream at Boryeong, Chungnam Province in Korea. After 13 months, remaining mass of leaves, culms and rhizomes was 16.9, 14.7, and 10.1%, respectively. Mass loss of the rhizomes was higher than those of the leaves and culms. The decay rate of leaves, culms and rhizomes was 1.86, 2.00 and 2.36 per year, respectively. Initial concentration of N, P, K, Ca, and Mg of leaves, culms and rhizomes was 18.0, 14.5, 44.0 mg/g for N,0.18, 0.12, 0.67 mg/g for P, 14.1 ,14.2, 14.8 mg/g for K,3.6, 1.5, 0.3 mg/g for Ca, 1.1, 0.5, 0.5 mg/g for Mg, respectively. Concentrations of N and P in rhizomes were higher than those in leaves and culms. Except for Mg in rhizomes, there was no immobilization period during the decomposition. Most of the N, P and Mg were lost during the first 3 months. In case of K and Ca, most were lost within 1 month.

• The Korean Journal of Ecology
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• 제23권2호
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• pp.157-161
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• 2000

Mass loss and changes of mineral nutrients during decomposition of Phragmites communis for 13 months from November 1998 to December 1999, were investigated at the fringe of stream at Boryeong, Chungnam Province in Korea. Plant materials, which were collected in November 1998. were divided into leaves, culms and rhizomes. Litterbags, 15${\times}$15 cm, were made of nylon mesh with 2-mm$^2$ holes. At 13 months after installation, remaining mass of leaves, culms and rhizomes was 29.0%, 57.4%, 20.6%, respectively. Mass loss rate of the culms was significantly lower than those of the leaves and rhizomes. The decay rate of leaves, culms and rhizomes was 1.21. 0.42 and 1.48 per year, respectively. Initial concentration of N, P, K, Ca and Mg of leaves. culms and rhizomes was 22.5, 9.0, 15.5 mg/g for N, 0.34. 0.10, 0.33 mg/g for P, 15.0, 12.5. 12.3 mg/g for K, 2.84. 0.80, 0.03 mg/g for Ca. 1.94. 0.97, 0.40 mg/g for Mg, respectively. Concentrations of nutrients were higher in leaves than in culms and rhizomes. Except for N and Mg in rhizomes, there was no immobilization period during the decomposition. In the case of remaining K and Ca, most are lost during the first 3 months. Without any suitable method for removal of dead part, eutrophication of freshwater may be accelerated by dead macrophytes.

• 식물조직배양학회지
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• 제25권3호
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• pp.183-187
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• 1998

한란(Cymbidium kanran)의 근경에 돌연변이 물질인 EMS를 처리하여 엽록소변이 식물체를 유도하였다. 한란종자를 발아시켜서 근경을 유도할 때 종자에 40분간 초음파 처리한 것이 무처리구에 비하여 발아율이 5.5배 정도 높았다. EMS 0.2%를 포함한 근경증식용 액체배지에서 3주간 배양하였을 경우에 50-60%정도의 근경이 갈변되었다. 갈변된 근경을 근경증식용 고체배지에서 배양하였을 경우에 근경조직의 일부분으로부터 새로운 근경의 증식의 관찰되었고, 이러한 근경조직을 절단하여 1년간 계단배양하던중에 분열조직의 일부분에서 색소변이 근경이 관찰되었다. 색소변이 근경은 나선형의 줄무늬가 가장 많았고, 그 외 엷은 노란색과 흰색의 근경이 분리되었다. 나선형의 근경에서 유도된 식물체는 잎에 무늬가 전혀 없는 녹색의 식물체 또는 줄무늬가 들어 있는 식물체가 유도되었다. 그러나 엷은 노란색 근경에서 유도된 식물체는 노란색 줄무늬가 있는 식물체로, 흰색 근경으로부터 유도된 식물체는 백화된 식물체로만 분화하였다. 이상의 결과 한란근경을 이용한 색소변이식물체의 유도에 있어서 배양중인 근경에 EMS처리가 효과적인 것을 보여주었다. 이러한 방법을 다른 식물에 적용하면 색소변이 식물체를 개발할 수 있으리라 생각된다.

• Journal of Plant Biotechnology
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• 제45권3호
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• pp.273-278
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• 2018

We performed an analysis of the effect of postharvest treatment of rhizomes of Aconitum septentrionale Koelle with the biological preparation of Bacispecin based on a cytokinin producing strain of Paenibacillus ehimensis IB-739 on the content of alkaloid lappaconitine in rhizomes. The total alkaloid content was assayed based on exhaustive extraction method. The lappaconitine content was measured by HPLC. The efficiency of the preparation was dependant on either the concentration or intensity of the growth processes in rhizomes. Both parameters varied depending on the stage of development of plants and the amount of precipitation. In the years with normal precipitation, concentrations of Bacispecin ranging from 5 to 10 g/l were found to be most effective for increasing lappaconitine content in rhizomes of A. septentrionale. Whereas, under local drought conditions, the concentration of preparation should be less than 2 g/l.

• 식물조직배양학회지
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• 제25권6호
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• pp.515-518
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• 1998

Ethylene이 옥화란 rhizome의 증식과 유식물체의 분화에 미치는 영향을 조사하였다. Ethylene 발생량은 rhizome 치상 8일째 가장 많았고 배양기간 동안의 총 ethylene 발생량은 rhizome이 증식될 때보다 유식물체가 분화될 때 많았다. 배양 rhizome에 ethephon을 처리한 결과 ethephon은 rhizome의 증식과 유식물체의 길이생장은 억제하였으나 유식물체의 분화에는 효과적이었다. Aminoethoxyvinylglycine(AVG) 1mg/L 처리에서는 rhizome의 증식과 유식물체의 분화에는 효과적이었으나 10mg/L에서는 rhizome의 증식이 억제되었고 유식물체의 길이생장은 촉진되었다. $\textrm{AgNO}_{3}$처리는 rhizome의 증식과 유식물체의 분화를 억제하였다.