초록
Ethylene이 옥화란 rhizome의 증식과 유식물체의 분화에 미치는 영향을 조사하였다. Ethylene 발생량은 rhizome 치상 8일째 가장 많았고 배양기간 동안의 총 ethylene 발생량은 rhizome이 증식될 때보다 유식물체가 분화될 때 많았다. 배양 rhizome에 ethephon을 처리한 결과 ethephon은 rhizome의 증식과 유식물체의 길이생장은 억제하였으나 유식물체의 분화에는 효과적이었다. Aminoethoxyvinylglycine(AVG) 1mg/L 처리에서는 rhizome의 증식과 유식물체의 분화에는 효과적이었으나 10mg/L에서는 rhizome의 증식이 억제되었고 유식물체의 길이생장은 촉진되었다. $\textrm{AgNO}_{3}$처리는 rhizome의 증식과 유식물체의 분화를 억제하였다.
The effect of ethylene on the proliferation of rhizomes and plant regeneration were investigated from rhizome segment culture of Cymbidium niveo-marginatum. Ethylene levels in the rhizome culture vessels were reached a maximum after 8 days of culture; total amount of ethylene evolution was much on the initiation of shoot induction than of rhizome proliferation. The treatment with ethephon on rhizomes was inhibited in the proliferation of rhizome and the growth of shoot length; however, the treatment was effective on shoot induction from rhizomes. Aminoethoxyvinylglycine(AVG) 1mg/L was effective on the proliferation of rhizomes and shoot induction from them; however, the proliferation of them was inhibited, and the growth of shoot length was significantly promoted at the concentration of 10mg/L AVG. The presence of $\textrm{AgNO}_{3}$ inhibited in the proliferation of rhizomes and shoot induction from them.
